BUSCADOR RECOLECTA

Peer reviewed

El dataset se puede consultar y descargar en el siguiente enlace https://doi.org/10.5281/zenodo.10934410, Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remainsmostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes inGA levels and perception during development.Weused this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM., Peer reviewed

Terrestrial ecosystems affect climate by reflecting solar irradiation, evaporative cooling, and carbon sequestration. Yet, little is known about how plant traits affect climate regulation processes in different habitat types. To study the links between the plot-level composition of plant communities and the satellite-based observations of climate regulation processes, we compiled the climate-adjusted proportion of reflected solar irradiation, evapotranspiration, and net primary productivity across 36,630 grid cells at the European extent, classified into ten types of forest, shrubland and grassland habitats and appended with the bioclimatic variables from the CHELSA Climatologies., Peer reviewed

Figure S1: Shear stress (Pa) vs. Shear rate (1/s) plot. Characterized shear-thinning behavior and determined viscosity parameters for all inks. (A) ALG5-GEL5; (B) ALG5-GEL5-OHAp1; (C) ALG5-GEL5-OHAp5; (D) ALG5-GEL5-OHAp10. ALG: Alginate, GEL: Gelatin, OHAp: Hydroxyapatite.; Figure S2: Determination of the pressure at the tip and the cartridge in bioinks. (A,B) ALG5-GEL5; (C,D) ALG5-GEL5-OHAp1; (E,F) ALG5-GEL5OHAp5; (G,H) ALG5-GEL5-OHAp10. ALG: Alginate, GEL: Gelatin, OHAp: Hydroxyapatite., Peer reviewed

Wildfires directly emit 2.1 Pg carbon (C) to the atmosphere annually. The net effect of wildfires on the C cycle, however, involves many interacting source and sink processes beyond these emissions from combustion. Among those, the role of post-fire enhanced soil organic carbon (SOC) erosion as a C sink mechanism remains essentially unquantified. Wildfires can greatly enhance soil erosion due to the loss of protective vegetation cover and changes to soil structure and wettability. Post-fire SOC erosion acts as a C sink when off-site burial and stabilization of C eroded after a fire, together with the on-site recovery of SOC content, exceed the C losses during its post-fire transport. Here we synthesize published data on post-fire SOC erosion and evaluate its overall potential to act as longer-term C sink. To explore its quantitative importance, we also model its magnitude at continental scale using the 2017 wildfire season in Europe. Our estimations show that the C sink ability of SOC water erosion during the first post-fire year could account for around 13% of the C emissions produced by wildland fires. This indicates that post-fire SOC erosion is a quantitatively important process in the overall C balance of fires, and highlights the need for more field data to further validate this initial assessment. Here we provide the post-fire SOC erosion dataset ("Post-fire SOC erosion rates" file) used for calculating the SOC ratio of eroded sediments implemented in the RUSLE modelling; as well as the list of data sources ("List of data sources" file)., Ministerio de Ciencia, Innovación y Universidades, Ramón y Cajal 2021 RYC2021-031262-I; European Union, Ramón y Cajal 2021 RYC2021-031262-I; Consejo Superior de Investigaciones Científicas, Proyecto Intramural Especial20208AT007; Natural Environment Research Council, UK-FDRS (NE/T003553/1) NE/T003553/1; European Union, FirEURisk101003890, Peer reviewed

Table S1: Supplementary data. Gene expression of genes related to the endocannabinoid system one week after treatment with saline (n = 3–6) or a single dose of cisplatin (i.p., 5 mg/kg; n = 2–6) in gastrointestinal tissue (antrum, fundus, ileum, and distal colon). The mean percentage of the saline-treated group (2−ΔCT) for saline is expressed as Mean ± SEM of saline- vs. cisplatin-treated animals. t-test: * significant difference; Table S2. Supplementary data. Gene expression of genes related to the endocannabinoid system one week after treatment with saline (n = 4–6) or a single dose of cisplatin (i.p., 5 mg/kg; n = 3–6) in nervous tissue (dorsal root ganglia L4 and L5, prefrontal cortex, periaqueductal grey and amygdala). The mean percentage of the saline-treated group (2−ΔCT) for saline is expressed as Mean ± SEM of saline- vs. cisplatin-treated animals. t-test: * significant difference., Peer reviewed

1. An ongoing restoration challenge is to recover native communities after the removal of invasive species. Because priority effects (i.e., the order and timing of species arrival) can strongly determine the trajectory of community assembly, their intentional manipulation is gaining attention to manage invasive plants and achieve restoration goals. Yet, ecologists and conservationists rarely consider how the order and timing of species removal inverse priority effect may impact future plant communities., 2. Here, we evaluated the dependence of community reassembly on inverse priority effects by experimentally removing the target invasives Sweetbriar rose (Rosa rubiginosa) and Scotch broom (Cytisus scoparius) in field and mesocosm communities. We manipulated removal order (rose-before-broom vs. broom-before-rose) and timing (simultaneously early vs. simultaneously late in the season). We performed a Hierarchical Modeling of Species Community to assess differences in community structure in response to order and timing of removal, and to evaluate whether species origin and leaf and seed traits were associated with species responses., 3. We found that the order of removal was as important as timing driving community reassembly. Simultaneous removal favoured nonnatives, more so when performed early. Sequential removals led to contrasting communities. Rose-before-broom removal also favoured nonnative grasses at expense of native species, whereas the inverse order produced small changes in communities. In general, species with high specific leaf area were boosted, regardless of their seed size., 4. Synthesis and applications. Inverse priority effects are neglected mechanisms that can drive variability in the reassembly of plant communities and can potentially upgrade invasive species management. These historical contingencies suggest the existence of an optimal order of removal that facilitates the recovery of the native community. We found that simultaneous removal promoted secondary invasions to a greater extent than sequential removals. Furthermore, removal order affected post-removal community structure. In our system, we suggest removing the rose before the broom to hinder nonnatives and pave the way for restoration of native communities. Our results show that manipulation of the order and timing of removal can help to achieve restoration goals., Peer reviewed

Peer reviewed

Knowing the location and movements of individuals at various temporal and spatial scales is an important facet of behavior and ecology. In threatened populations, movements that would ensure gene flow and population viability are often challenged by habitat fragmentation. Also in those endangered populations capturing and handling individuals to tag them, or to obtain tissue samples, can present additional challenges. DNA tagging, i.e. non-invasive individual identification of samples, can reveal movement patterns. We used fecal material genetically assigned to individuals to indirectly track movements of a large-bodied, endangered forest bird, Cantabrian capercaillie (Tetrao urogallus cantabricus). We wanted to know how the birds were using the fragmented forest landscape, and whether they showed fidelity to display areas. We used multi-event capture-recapture models to estimate fidelity to display areas among three consecutive mating seasons. We identified 127 individuals, and registered movements of 22 females and 48 males. Most observed movements were as expected relatively short, concentrated around display areas. We did not find differences in movement distances between females and males within mating seasons, or between them. Fidelity to display areas among seasons was 0.62 (± 0.12 SE) for females and 0.77 (± 0.07 SE) for males. The best CR model suggested no sex or season effects. Several longer movements, up to 9.9 km, linked distant display areas, demonstrating that Cantabrian capercaillies were able to move between different parts of the study area, complementing previous studies on gene flow. Those longer movements may be taking birds out of the study area, and into historical capercaillie territories, which still include substantial forest cover. The non-invasive DNA tagging approach provided a much larger sample size than would have been feasible with direct tracking. Lack of information on the social status of individuals and timing of movements are some disadvantages of DNA tagging., Ministerio de Ciencia e Innovación : CGL2010-15990; Foundation for the Promotion of Applied Scientific Research and Technology in Asturias : IB08-158; Foundation for the Promotion of Applied Scientific Research and Technology in Asturias : AYUD/2021/51261, Peer reviewed

Bacterial interactions are vital for adapting to changing environments, with quorum sensing (QS) systems playing a central role in coordinating behaviors through small signaling molecules. The RRNPPA family is the prevalent QS systems in Bacillota and mediating communication through secreted oligopeptides, which are processed into active pheromones by extracellular proteases. Notably, in several cases the propeptides show the presence of multiple putative pheromones within their sequences, which has been proposed as a mechanism to diversify peptide-receptor specificity and potentially facilitate new functions. However, neither the processes governing the maturation of propeptides containing multiple pheromones, nor their functional significance has been evaluated. Here, using 2 Rap systems from bacteriophages infecting Bacillus subtilis that exhibit different types of pheromone duplication in their propeptides, we investigate the maturation process and the molecular and functional activities of the produced pheromones. Our results reveal that distinct maturation processes generate multiple mature pheromones, which bind to receptors with varying affinities but produce identical structural and biological responses. These findings add additional layers in the complexity of QS communication and regulation, opening new possibilities for microbial social behaviors, highlighting the intricate nature of bacterial interactions and adaptation., pbio.3002744.s017.xlsx, Peer reviewed