BUSCADOR RECOLECTA

Lateral views of adult eyes (a-c) and L3 eye discs, stained with Dcp-1 (and counterstained with rhodamine phalloidin, “actin”) (a’-c’) of the indicated genotypes. (d) Distribution of apoptosis intensity in L3 discs of the indicated genotypes. Blocking apoptosis driving the UAS-RHG-RNAi reduces the apoptosis caused by tkv attenuation (optix>tkvRI + RHGRI). The statistical analysis corresponding to these data can be found in S2 Table. (*): p < 0.05; (**): p < 0.01; (***): p < 0.001. The data underlying the graphs shown in the figure can be found in “Fig 2_data” in the Supporting information file S1 Raw Data., Peer reviewed

1 file, This dataset comprises input data from INTECMAR and the predicted outcomes, The authors gratefully acknowledge the financial support by the Programa de axudas á etapa predoutoral da Xunta de Galicia (Axencia Galega de Innovación) (Grant nº IN606A-2022/025). F.F.P. and A.V. were supported by REDEIRA (TED2021-132188B-I00) project, funded by MCIN/AEI/10.13039/501100011033, Peer reviewed

This file describes in detail the statistical methods used for data analysis., Peer reviewed

Each genotype effect on %Apop median, their p.values, and the predicted median for each of them are shown (see main text, Materials and methods section)., Peer reviewed

First block shows each genotype’s effect on the rE median, their p.values, and the predicted median for each of them. The second and third blocks show the standard deviation of each group for rE and sFAi, respectively, and the result of the comparison of variances of each group against the optix>+ reference group., Peer reviewed

(a, b) Heads from GMR>+ (a) and GMR>Upd (b) adult males; frontal view. While the median eye size (rE: c) of GMR>Upd flies is approximately 1.4 times that of control (GMR>+) ones (statistically significant), the fluctuating asymmetry index (“precision”) (FAi: d) is indistinguishable between the 2 genotypes. Female (F) and male (M) distributions are shown. (e, f) Eye primordia stained for the apoptotic marker Dcp-1. Apoptotic signal is observed in both GMR>Upd as well as in GMR>+ control primordia. The data used in the graphs shown in the figure can be found in “S1_Fig 4_data” in the Supporting information file S1 Raw Data., Peer reviewed

(a, b) Representative late L3 eye imaginal discs, stained for the mitotic marker phosphor-histone H3 (PH3), and counterstained with rhodamine phalloidin (“actin”). (c) Distribution of mitotic rate, measured as the rate of PH3-positive area relative to the % of available area (nonapoptotic) in the anterior domain (regions outlined in yellow) in the 2 genotypes (see S1 Statistical Methods). Bayes Factor (BF) value close to 0 allows accepting the null hypothesis: “There is no significant differences between genotypes.” BF = 0.37. The bar indicates an 80% power to detect differences. This analysis indicates that mitotic rate of progenitors does not change in optix>tkvRI relative to optix>+ controls (see S1 Statistical Methods). The data used in the graphs shown in the figure can be found in “S1_Fig 2_data_A” and “S1_Fig 2_data_B” in the Supporting information file S1 Raw Data., Peer reviewed

The intraindividual FA (FAi) is calculated using the left and right eyes from the same individual. The interindividual FA (FAn) is calculated as the difference between the left eye of an individual and the right eye randomly chosen from the population of measured eyes. The difference between FAn and FAi was computed for each fly (ΔFA). If there were no “individual” effect, ΔFA should be 0. The effect of each genotype on ΔFA median, their p.values, and the predicted median for each of them are shown (see S1 Statistical Methods). The ΔFA median is slightly, though significantly greater than 0 across all genotypes relative to the control (optix>+). This result indicates that eye size within an individual is slightly correlated irrespective of genotype. The data used in the graphs shown in the figure can be found in “Fig 1_S6Fig 1_data” in the Supporting information file S1 Raw Data., Peer reviewed

L3 dpp-Z discs (a, b), stained for apoptosis (dcp-1, green), dpp transcription (beta-galactosidase, red), and the retinal differentiation marker Elav (blue). (a) Control (“+”: Oregon-R) and (b) Bar mutant discs. The ellipse approximately outlines the eye region. “a” marks the antennal primordium., Peer reviewed

Results are shown for rE and sFAi of eyes from individuals of the following genotypes: “+”: Oregon-R (Or-R) wild-type strain; “optix>+”: Progeny from the cross optix-GAL4 to Or-R; “optix>UAS-CherryRI”: The optix-GAL4; UAS-Cherry_RNAi progeny obtained by crossing optix-GAL4 to UAS-Cherry_RNAi. This dataset was obtained independently of the datasets included in Fig 2 of the main text. The median rE of optix>CherryRI eyes is slightly higher than that of the optix>+ reference control group; however, it is not significantly higher than that of the wild-type Or-R group (a). There are no significant differences in sFAi between any of the groups compared (b). The table shows the statistical analysis of rE and sFAi among the different groups considered. This analysis concludes that there is no significant effect of driving a nonspecific, neutral RNAi on eye size precision and that the effect on final eye size is within the variability present among the alternative control lines used (An equivalent table is explained in S2 Table. Further details on the statistical analysis can be found in the main text Materials and methods section). The data underlying the graphs shown in the figure can be found in “S3_Fig 1_data” in the Supporting information file S1 Raw Data., Peer reviewed