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This publication contains the original data supporting our results concerning the application of biphasic pulsed electrical stimulation on MG-63 cells. We aim to discriminate between effects of the electric field and electrochemically conditioned medium. The dataset consists of the original output files, excel sheets to consolidate the information for the experiments as well as Readme.txt files with most important information concerning the data. For additional information on the material and methods as well as results see the corresponding research article., This research was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), grant number SFB ELAINE, 1270/1,2-299150580. We also acknowledge the funding from Atracción de Talento Programme, Modalidad-1 Ref. 2019-T1/IND-1335 and the grant PID2021-128611OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe., Peer reviewed

12 páginas, 8 figuras de información suplementaria., Peer reviewed

Peer reviewed

Supplemental Figure S1. Correlation between branch length and diameter for diploid (purple), triploid (brown), and tetraploid (blue) trees grown on their own roots in the field. Supplemental Figure S2. Leaf phenology of diploid (pink), triploid (brown), and tetraploid (blue) grafted atemoya genotypes grown in the greenhouse. Supplemental Figure S3. Aboveground organ measurement in grafted scions of diploid (purple), triploid (brown), and tetraploid (blue) atemoya trees grown in the greenhouse. Supplemental Figure S4. Correlation between SI and leaf area in diploid (pink), triploid (brown), and tetraploid (blue) grafted atemoya polyploids. Supplemental Figure S5. Vessel element radii (logarithmic scale) in the continuum formed by stems of decreasing diameters (circles), leaf veins of increasing order (triangles), and flower pedicels (ribbons) of diploid (pink), triploid (brown), and tetraploid (blue). Supplemental Figure S6. Relative humidity (blue) and temperature (red) in the greenhouse during 2019 and 2020. Supplemental Figure S7. Total leaf number per branch in diploid (pink), triploid (brown), and tetraploid (blue) atemoya scions at the end of the growth season (June 2020), as a function of treatment. Supplemental Figure S8. Stomatal conductance of diploid (pink), triploid (brown), and tetraploid (blue) grafted atemoya genotypes grown in the greenhouse under three irrigation regimes. Supplemental Table S1. Gas exchange variables., J.M.L. was financed by a ComFuturo Project from the Fundación General CSIC (FGCSIC), 2 RTI Projects (100-900-0000/AEI/10.13039/501100011033 and PID2021-129074OB-I00) from the Agencia Estatal de Investigación (AEI)-Ministerio de Ciencia e Innovación (MCINN), Spain, and a LINKB20067 from Consejo Superior de Investigaciones Científicas (CSIC). J.I.H. was supported by PID2019-109566RB-I00 funded by AEI-MICINN and ERDF A way to make Europe (MCIN/AEI/10.13039/501100011033). Open access was partially funded by CSIC and the Project QUAL21 012 IHSM from Consejería de Universidad, Investigación e Innovación, Junta de Andalucía., Peer reviewed

Table S1: Response of the Lens germplasm accessions to U. viciae-fabae ex L. culinaris studied. DS = Disease Severity (%); IT = Infection Type according Stakman et al. (1962) [29], 6 where IT 0 = no symptoms, IT ; = necrotic flecks, IT 1 = minute pustules barely sporulating, IT 2 = necrotic halo surrounding small pustules, IT 3 = chlorotic halo and IT 4 = well-formed pustules with 7 no associated chlorosis or necrosis. 8 Ns = not studied, This research was funded by the Spanish Agencia Estatal de Investigación (AEI) grant PID2020-114668RB-100., Peer reviewed

7646220.zip contains:, 1) TIP_data folder: INDICA_teinsertions_5_2_2_matrix_sorted_ID_MAF003.txt: TIP matrix used for TIP-eQTL mapping (binary form, where 0 = absence, 1= presence) // INDICA_teinsertions_5_2_2_matrix_sorted_ID_MAF003.info: TIP information (chrom,start,superfamily,family,maf) // JAPONICA_teinsertions_5_2_2_matrix_sorted_ID_MAF003.txt: TIP matrix used for TIP-eQTL mapping (binary form, where 0 = absence, 1= presence) // JAPONICA_teinsertions_5_2_2_matrix_sorted_ID_MAF003.info:TIP information (chrom,start,superfamily,family,maf), 2) SNP_data folder: SNP_matrix_final_IRGC_Indica_MAF003.lfmm: SNP binary matrix used for TIP-eQTL mapping // SNP_matrix_final_IRGC_Indica_MAF003.info: SNP information // SNP_matrix_final_IRGC_japonica_MAF003.lfmm: SNP binary matrix used for TIP-eQTL mapping // SNP_matrix_final_IRGC_japonica_MAF003.info:SNP information, 3) Expression data: Normalized expression data and accessory files used for TIP-eQTL analysis (as described in TIP-eQTL_matrix.R), Code: PopoolationTE2.sh: Bash script to reproduce PopoolationTE2 analysis (TIP detection) // TIP-eQTL_matrix.R: R code to reproduce eQTL analyses, Peer reviewed

Data used in the experimental study carried out in early life stages of the seahorse Hippocampus reidi to assess the influence of diet shift on changes and turnover rates and discrimination factors for carbon (δ13C) and nitrogen (δ15N) stable isotopes in juveniles. Newborn seahorses were fed for 60 days following two feeding schedules (A6 and A11) based initially on copepods Acartia tonsa and subsequently on Artemia nauplii (from days 6 and 11 onwards, respectively). Isotopic data correspond to raw data normalized for lipids, No

Data used in "Avian sibling cannibalism: Hoopoe mothers regularly use their last hatched nestlings to feed older siblings", Peer reviewed

Additional file 1: Table S1. Sample size of avian nests used to estimate prevalence of extra-pair paternity of the 60 bird species used in the study., Spanish Ministerio de Ciencia, Innovación y Universidades and European (FEDER), Peer reviewed

Fig. S1: (a) Northern Hemisphere regions with mean annual temperature (MAT) ＜4.9℃ during the period of 1970-2000 (data from worldclim.org) and (b) Northern Hemisphere regions with CMIP5 projected MAT ＞4.9℃ during the period of 2061-2080.-- Fig. S2: Xylem growth rings of Norway spruce (Picea abies) in the middle of the growing season showing tracheids at different differentiation stages. Just below the cambium (CC) are cells in the enlargement stage (PC), followed by cells in the phase of secondary wall formation and wall-thickening (SW), and then mature cells (MT).-- Figure S3. Variable importance from the boosted regression tree (a) showing the marginal effect of the predictor on the cell-wall-thickening DOY probability. These predictors were ranked by their relative importance; (b-g) Partial dependence plots of cell-wall-thickening DOY (day of the year) on mean annual temperature (MAT), photoperiod, chilling, forcing, scPDSI, and spring temperature variation (Temp_variation).-- Figure S4: Variation in photoperiod (a), chilling (b), forcing (c), scPDSI (d), spring temperature variance (e), and frost frequency (f), according to the mean annual temperature (MAT) at the sites. Regression lines represent the predicted values estimated by natural cubic splines. Shadowed areas indicate 95% confidence interval. Biomes include boreal (B), temperate (T), Mediterranean (M), and subtropical (S).-- Figure S5: Boxplots, with jitter, displaying the distribution of selected tree species ranging across the mean annual temperature (MAT) gradients. Species are reported with the following acronyms and classified into early (JUPR, Juniperus przewalskii; JUTH, Juniperus thurifera; LADE, Larix decidua; PIHA, Pinus halepensis,; PIHE, Pinus heldreichii; PILE, Pinus leucodermis; PILO, Pinus longaeva; PIMA, Pinus massoniana; PIPE, Pinus peuce; PIPI, Pinus pinaster; PISY, Pinus sylvestris; PITA, Pinus tabulaeformis; and PIUN, Pinus uncina) and late (ABAL, Abies alba; ABBA, Abies balsamea; ABGE, Abies georgei; CELI, Cedrus libani; PCAB, Picea abies; PCMA, Picea mariana; PICE, Pinus cembra) successional species types (see Species classification Materials and Methods).-- Figure S6: Changes in the cell-wall-thickening DOY (day of the year) for Picea abies (PCAB) along the mean annual temperature (MAT) gradients of sites, as fitted by a linear mixed effect model with sites as the random effect.-- Figure S7: Changes in cell-wall-thickening DOY (day of the year) along the mean annual temperature (MAT) gradients of the sites (a) between the cold and warm sites classified by the transition temperature at MAT=4.9±1.1℃ across biomes: boreal sites excluded the few dots above 4.9 ℃ (intercept: 167.86, slope: -4.96), cold-temperate sites (MAT below 4.9 ℃; intercept: 178.68, slope: -5.24), warm-temperate sites (MAT above 4.9 ℃; intercept: 183.22, slope: -4.04); Mediterranean sites excluded the few dots below 4.9 ℃ (intercept: 147.60, slope: -0.515).-- Figure S8: Summary of the direction and magnitude of the effect of multiple predictors on cell-wall-thickening DOY (day of the year) in different biomes at cold or warm sites, as fitted by linear mixed effect models. The upper (a, b) and middle (c,d) panels were from cold and warm sites, respectively. Significant effects occur when no overlaps exist between the 95% error bars and zero; the blue and red colors denote the positive versus negative effects, respectively. Variance partitioning for each model indicating the relative importance of each predictor is also shown in e, and the sample sizes for each model are reported. B_Cold: boreal sites excluded the few dots above 4.9 ℃; T_Cold: temperate sites with MAT below 4.9 ℃; T_Warm: temperate sites with MAT above 4.9 ℃; M_Warm: Mediterranean sites excluded the few dots with MAT below 4.9 ℃.-- Figure S9: Variations (violin plots) and distributions (nested boxplots) of frost frequency and local spring temperature variation for early versus late successional species in the cold (a and c) and warm zones (b and d). Cold and warm sites were classified by the transition temperature at a mean annual temperature of MAT=4.9±1.1℃. E: early successional species; L: late successional species.-- Table S1: The sites, species, and years included in the analysis. The species were reported with the following acronyms and classified into early (E) and late (L) successional species type: ABAL, Abies alba, L; ABBA, Abies balsamea, L; ABGE, Abies georgei, L; CELI, Cedrus libani, L; JUPR, Juniperus przewalskii, E; JUTH, Juniperus thurifera, E; LADE, Larix decidua, E; PCAB, Picea abies, L; PCMA, Picea mariana, L; PICE, Pinus cembra, L; PIHA, Pinus halepensis, E; PIHE, Pinus heldreichii, E; PILE, Pinus leucodermis, E; PILO, Pinus longaeva, E; PIMA, Pinus massoniana, E; PIPE, Pinus peuce, E; PIPI, Pinus pinaster, E; PISY, Pinus sylvestris, E; PITA, Pinus tabulaeformis, E; and PIUN, Pinus uncinata, E. The entire study area was divided into subtropical (S), Mediterranean (M), temperate (T), and boreal (B) biomes. The site temperature for each site was computed as the average of the mean annual temperatures (MATs) across all years providing observations for the site. Sites with climate data obtained from nearby weather stations are indicated by *.-- Table S2. Descriptive summary statistics of cell-wall-thickening DOY (day of year) for all observations, for observations at cold sites, and for observations at warm sites.-- Table S3. Summary of linear mixed modeling of the effects of selected predictors on cell-wall-thickening DOY (day of year); CI: confidence interval.-- Table S4: Variance inflation factor (vif) of each predictor variable in the linear mixed models for all observations and subset modelings corresponding to the results shown in Fig. 3., Peer reviewed