BUSCADOR RECOLECTA

(A) Above: Sequence motif enriched in C. elegans HE cluster HMT promoters, relative to other HMTs promoters. Below: previously reported EFL-2 binding motif. (B) Binding of the C. elegans Retinoblastoma orthologue LIN-35 upstream of the TSS of the HE cluster, other HMT genes, and random genes; p-values from Wilcoxon test. (C) Enrichment for transcription factor binding, from ENCODE ChIP-seq experiments, upstream of human HMT genes. Odds ratios and p-value derived from Fisher’s exact test. (D) Boxplots show median total HMT or NNMT expression percentile drawn from 1,000 iterations of pan-cancer sampling of tumours with wild-type RB1 or potentially deleterious RB1 mutations; p-value derived from t test. (E) Total HMT expression in small cell lung cancer cell lines from the CCLE with wild-type RB1 or deleterious RB1 mutations; p-value derived from t test. (F) Estimated E2F1 activity vs. total HMT expression (both corrected for confounders) in breast cancer primary tumours from the TCGA. (G) Potential architectures of the GRN linking RB1, NNMT, and HMTs. (H) Linear model t-values explaining total HMT and NNMT expression for RB1 mutation status as the sole explanatory variable or jointly considered with NNMT/HMT expression respectively; p-value derived from t test. Underlying data for all panels can be found in https://zenodo.org/record/8383542. CCLE, Cancer Cell Line Encyclopedia; GRN, gene regulatory network; HMT, histone methyltransferase; NNMT, nicotinamide N-methyltransferase; Rb, Retinoblastoma; TCGA, The Cancer Genome Atlas; TSS, transcription start site., Peer reviewed

Individual HMT levels in Rb-mutant tumours in TCGA., Peer reviewed

The N-terminal tails of eukaryotic histones are frequently posttranslationally modified. The role of these modifications in transcriptional regulation is well-documented. However, the extent to which the enzymatic processes of histone posttranslational modification might affect metabolic regulation is less clear. Here, we investigated how histone methylation might affect metabolism using metabolomics, proteomics, and RNA-seq data from cancer cell lines, primary tumour samples and healthy tissue samples. In cancer, the expression of histone methyltransferases (HMTs) was inversely correlated to the activity of NNMT, an enzyme previously characterised as a methyl sink that disposes of excess methyl groups carried by the universal methyl donor S-adenosyl methionine (SAM or AdoMet). In healthy tissues, histone methylation was inversely correlated to the levels of an alternative methyl sink, PEMT. These associations affected the levels of multiple histone marks on chromatin genome-wide but had no detectable impact on transcriptional regulation. We show that HMTs with a variety of different associations to transcription are co-regulated by the Retinoblastoma (Rb) tumour suppressor in human cells. Rb-mutant cancers show increased total HMT activity and down-regulation of NNMT. Together, our results suggest that the total activity of HMTs affects SAM metabolism, independent of transcriptional regulation., Peer reviewed

1 file, The Cape Verde Frontal Zone (CVFZ) is a highly dynamic region located in the southern boundary of the Canary Current Eastern Boundary Upwelling Ecosystem. Due to the interaction of the Cape Verde Front with the Mauritanian coastal upwelling, the area features large vertical and horizontal export fluxes of organic matter. Full-depth profiles were recorded during FLUXES I cruise, with four consecutive transects defining a box embracing the giant filament of Cape Blanc and the Cape Verde front. Fifteen levels were sampled in medium and long stations (down to 4000 dbar) and 10 levels in short stations (down to 2000 dbar) where inorganic nutrients (NO3, NO2, Si(OH)4 and PO4), dissolved organic carbon/total dissolved nitrogen (DOC/TDN), and suspended particulate organic carbon and nitrogen (POC and PON) were sampled. Micromolar concentrations of nutrient salts were determined simultaneously by segmented flow analysis in an Alliance Futura autoanalyser. The determination of suspended POC and PON was carried out by high temperature catalytic oxidation at 900 °C in a Perkin Elmer 2400 elemental analyser. DOC and TDN were analysed by high temperature catalytic oxidation at 680 °C with a Shimadzu TOC-V analyser connected in line with a TNM1 measuring unit. Alongside with water samples conductivity, temperature and depth (CTD; SeaBird SBE911 plus), and dissolved oxygen (SeaBird SBE43), fluorescence of chlorophyll (SeaPoint SCF), and turbidity (SeaPoint STM) were measured. CTD conductivity was calibrated with water samples taken from the rosette and analysed on board with a Guildline 8410-A Portasal salinometer. Samples for dissolved oxygen (O2) determination were analysed on board by the Winkler potentiometric method. The chlorophyll (Chl) fluorescence sensor was calibrated with water samples taken at 4 depths in the photic layer which were estimated fluorometrically by means of a Turner Designs bench fluorometer 10-AU, Horizon 2020 (H2020), grant/award no. 817806: Sustainable management of mesopelagic resources; Ministerio de Ciencia e Innovación (MICINN), grant/award no. CTM2015-69392-C3: Constraining organic carbon fluxes in an eastern boundary upwelling ecosystem (NW Africa): the role of non-sinking carbon in the context of the biological pump; Ministerio de Ciencia e Innovación (MICINN), grant/award no. PID2019-109084RB-C21: Biogeochemical impact of mesoscale and sub-mesoscale processes along the life history of cyclonic and anticyclonic eddies: plankton variability and productivity, Peer reviewed

GTEx NNMT-HMT correlation all tissues full labelled plots., Peer reviewed

TCGA NNMT-HMT correlation all cancers full labelled plots., Peer reviewed

CCLE NNMT-HMT correlation all cancers full labelled plots., Peer reviewed

Subcellular localisations of transsulphuration and glutathione synthesis genes., Peer reviewed

HMT correlations to transcription factor activity in TCGA., Peer reviewed

1 file, To evaluate changes in terrestrial and aquatic ecosystems in the Azores, we developed paleoecological and paleoclimate records from Lake Funda on Flores Island that span the last millennium. Vegetation composition (n-alkane average chain length) indicate when human activities began in the catchment area and biogenic silica tracks changes in diatom productivity. In addition, sterol hydrogenation and archaeal lipids (isoprenoid glycerol dialkyl glycerol tetraethers) trace changes in redox conditions and biogeochemical cycles, respectively. Finally, a high-resolution reconstruction using leaf wax hydrogen isotopes records changes in precipitation amount over the last millennium, Fundação para a Ciência e Tecnologia (FCT), grant/award no. PTDC/CTA-AMB/28511/2017: WHEN WERE THE AZORES ARCHIPELAGO REALLY COLONIZED? A HIGH-RESOLUTION PALEOLIMNOLOGICAL APPROACH, Peer reviewed