BUSCADOR RECOLECTA

An exposure assessment model for industrial use has been developed by using kinetic data from inactivation and growth of Bacillus cereus spores. It can provide a valuable tool for estimating the concentration of B. cereus after a storage period of 24 h at a specified temperature (20 °C) and for an estimation of the percentage of contaminated portions according to the input data of the model. This model considers a rice-derived product that has undergone a standard cooking process at 95 °C for 20 min. According to the results, the presence of chitosan affects the final microbial load after storage, potentially serving as an additional control measure in the event of cold chain abuse or break. Chitosan’s antimicrobial properties likely play a role in reducing microbial growth during storage, thereby contributing to enhanced food safety. In practical terms, this suggests that incorporating chitosan into food products, especially those susceptible to microbial contamination like rice derivatives, could help mitigate risks associated with temperature abuse or cold chain disruptions. By acting as a protective barrier against microbial proliferation, chitosan offers a preventive measure to maintain product quality and safety throughout the supply chain. Considering two scenarios, 104 or 107 as initial contamination the model estimated that the 55 and 100% of portions would be respectively contaminated, according to a Performance Criteria of 4 log reductions., MCIN/AEI/10.13039/501100011033, “ERDF A way of making Europe” and by the “European Union’’ [grants PID-2020-11631RB-C31 and PRE-2021-098627] and Ministerio de Ciencia e Innovación, Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana [grant ACIF/2021/424]., Peer reviewed

S1 Fig. Analysis of the Fas2 GOF condition in adult flies and FLP-OUT clones. (A) Over-expression of Fas2TRM (UAS-fas2TRM) under the control of the Tub-GAL4/+ driver (Tub-GAL4>fas2GOF) caused a general reduction of body size. Compare the two females, with over-expression of Fas2TRM (top) and a normal CyO sibling (Tub-GAL4) control (bottom). (B) A/P compartment border in an en-GAL4 UAS-CD8GFP/UAS-fas2TRM UAS-fas2GPI pupal wing. The expression of Fas2GPI and Fas2TRM in the Posterior compartment of the wing (labelled with GFP) did not modify the size or shape of the cells. Bar: 50 μm. (C) FLP-OUT fas2 GOF clones in a puc-LacZ/+ genetic background did not show increased JNK activity nor apoptosis. At left, a wing disc with several Fas2 GOF clones (labeled with GFP) which cover most of the disc surface. The lack of ectopic expression from the reporter puc-LacZ reveals that the JNK pathway was not de-repressed in these clones. Staining with anti-cleaved Caspase 3 (Blue channel) did not reveal obvious signs of apoptosis in the wing discs either. Lower panels, note the presence of just three cells expressing cleaved Caspase 3 in one of the normal regions engulfed by the Fas2 GOF clones. Bar: 50 μm. (D) FLP-OUT y w hs-FLP; ActinFRTy+FRT-GAL4 UAS-GFP/UAS-fas2TRM UAS-fas2GPI; UAS-λEgfr/dpp-LacZ clones (middle panels) display the same phenotype than FLP-OUT y w hs-FLP; ActinFRTy+FRT-GAL4 UAS-GFP/+; UAS-λEgfr/dpp-LacZ clones (bottom panels). In both types of clones there is precocious non-cell autonomous retinal differentiation (arrows) and ectopic dpp expression inside the clone. Bar: 50 μm., S2 Fig. Fipi and Elff are expressed at the cell membrane and partially colocalize with Fas2 during late 3rd instar larva. (A) Top: the protein trap line fipi-EGFP-FLAG (RRID: BDSC_60532) shows protein colocalization with the membrane marker CD8-RFP. 3rd instar wing imaginal disc stained with anti-Flag (green) and expressing UAS-CD8-RFP under the control of the en-GAL4/+ driver. ImageJ Colocalization and Colocalization Finder plugins, ratio: 50.0; threshold red: 100.0; threshold green: 100.0; Pearson’s correlation: + 0,349. Bottom: the expression of fipi-EGFP-FLAG (RRID:BDSC_60532) (anti-Flag, green) partially colocalizes with Fas2 (Mab 1D4, red) in the wing imaginal disc during late 3rd instar larva. Expression of both proteins in different domains is better seen in the lateral views of cells at the imaginal disc folds. Note that the partial colocalization corresponds to the interface between Fas2 (red) and Fipi (green). ImageJ Colocalization and Colocalization Finder plugins, ratio: 50.0; threshold red: 100.0; threshold green: 100.0; Pearson’s correlation: + 0,332. Bar: 50 μm. (B) Top: the protein trap line elff-EGFP-FLAG (RRID:BDSC_60531) also shows colocalization with the membrane marker CD8-RFP. 3rd instar wing imaginal disc stained with anti-Flag (green) and expressing UAS-CD8-RFP under the control of the en-GAL/+4 driver. ImageJ Colocalization and Colocalization Finder plugins, ratio: 50.0; threshold red: 100.0; threshold green: 100.0; Pearson’s correlation: + 0,419. Bottom: elff-EGFP-FLAG (RRID:BDSC_60531) (anti-Flag, green) partially colocalizes with Fas2 (Mab 1D4, red) during late 3rd instar larva. The panel shows the cell profiles at folds near the prospective wing pouch. Note the colocalization at the apposition of red (Fas2) and green (Elff) signals. ImageJ Colocalization and Colocalization Finder plugins, ratio: 50.0; threshold red: 100.0; threshold green: 100.0; Pearson’s correlation: + 0,392., S3 Fig. fipi and elff RNAis efficiently suppress gene function. The results for fipi and elff RNAi inhibition presented in Fig 5C are here compared with the same combinations over a deficiency that removes the corresponding endogenous gene. The phenotype caused by UAS-fipi or UAS-elff RNAi expression driven by MS1096/+ is not significantly enhanced by reducing 50% the dose of the corresponding gene using Df(2L)BSC225, fipi−(RRID:BDSC_9702) and Df(2L)Exel7008, elff−(RRID:BDSC_7780). The results show that these RNAis block most fipi and elff function causing at least a strong hypomorphic condition. Wing size is area in μm2/103., S4 Fig. Over-expression of Fas2 enhances the EGFR activation caused by Vn over-expression. (A) The gain of function for EGFR (EgfrGOF, MS1096-GAL4/+; Egfrλtop/+; bottom left) during imaginal wing disc growth produces adult wings smaller than normal (WT, MS1096/+, top left), and with a pronounced differentiation of extra-vein territory. A similar situation is attained by the over-expression of the EGF-like ligand Vein (vnGOF; top right). Both, the reduction in wing size and the extra-vein phenotype caused by the over- expression of UAS-vn (under the control of the MS1096-GAL4/+ driver, vnGOF) is enhanced by the simultaneous over-expression of fas2 (vnGOF fas2GOF, MS1096/+; UAS-vn/UAS- fas2GPI UAS-fas2TRM; bottom right). (B) Quantification of wing area in over-expression conditions for Vn (vnGOF) and Vn plus Fas2GPI Fas2TRM (vnGOF G+T) under the control of the MS1096-GAL4/+ driver. All pictures heterozygous MS1096-GAL4/+ females raised at 25°C. Wing size is area in μm2/103, S5 Fig. fipi and elff LOF conditions are epistatic to the fas2 LOF. (A) Inhibition of fas2 (RNAi RRID: BDSC_28990) in combination with fipi (RNAi RRID:BDSC_42589, Ri2) or elff (RNAi RRID:VDRC_32576, Ri3) in MS1096-GAL4/+ females causes a normalized adult wing size. The double inhibition of fipi and elff expression in the fas2 LOF background shows a phenotype slightly suppressed. The combinations fipi elff are the same as in Fig 5C. All individuals are heterozygous MS1096-GAL4/+ females raised at 25°C. Wing size is area in μm2/103. (B) The inhibition of fas2 by the expression of RNAi (RRID:BDSC_28990) in MS1096-GAL4/+ females (fas2LOF) raised at 29°C does not produce alterations in the wing vein pattern (with the exception of missing cross-veins in some individuals). Simultaneous inhibition of fipi and elff (RNAi RRID:BDSC_42589 and RNAi RRID:VDRC_32576) in MS1096-GAL4/+ females (fipiRi2 elffRi3) raised at 29°C causes the differentiation of extra-vein tissue (arrowheads), while the simultaneous inhibition of fas2 fipi and elff expression (fipiRi2 elffRi3 fas2LOF) does not eliminate the formation of extra-veins (arrowheads). All pictures heterozygous MS1096-GAL4/+ females raised at 29°C., S1 File. Materials and methods. Strains used for MARCM and coupled-MARCM analysis., S2 File. Data and statistics., Peer reviewed

The study aimed to determine the degree of interaction by isobolographic analysis of co-administration in an OGD/R cell model using human SH-SY5Y neuroblastoma cells. To improve the therapeutic effect of both drugs when administered in combination. Highlights: 1. The glucose and oxygen deprivation paradigm with reoxygenation was established in the cell line SH-SY5Y. 2. Cells were tested for dapsone and cannabidiol cytotoxicity and neuroprotection concentrations. 3. Isobolographic analysis showed that dapsone and cannabidiol synergistically protect the SH-SY5Y cells. 4. In an OGD/R model in SH-SY5Y cells, DDS-CBD reduced LDH, ROS, and caspase 3. Instructions: All experiments were performed in triplicate, each repeated at least three times. In all cases, an exploratory data analysis was performed to determine whether or not there was a normal distribution, applying the Kolmogorov-Smirnov test and homogeneity of standard error, applying the Levene test. Once this was determined, parametric statistical tests such as one-way ANOVA, followed by Dunnett post hoc test and mortality in SH-SY5Y cells analysis, LC50 and EC50 values (with 95% confidence limits) were determined by Probit regression analyses. Data was analyzed using SPSS, version 25.0 software. The results of this study were performed in triplicate, with three repetitions, and were expressed as means ± SEM with A significance level of *p < 0.05, **p < 0.01, ***p < 0.001., -Figure 1A Mortality in SH-SY5Y cells after the administration of DDS and evaluated at different concentrations for 24 h. -Figure 1B Mortality in SH-SY5Y cells after CBD was evaluated at different concentrations for 24 h. -Figure 2A The effect of dapsone evaluated in an oxygen-glucose deprivation and reoxygenation OGD R model in SH-SY5Y cells. -Figure 2B the effect of cannabidiol evaluated in an oxygen-glucose deprivation and reoxygenation OGD R model in SH-SY5Y cells. -Figure 3A Effect of the combination of dapsone and cannabidiol at three different ratios cell viability. -Figure 3B Effect of the combination of DDS-CBD in a 1 4 ratio at different ratios. -Figure 4A Effect of DDS and CBD alone or combined which corresponds to the effective concentration 90 upon cell viability-PUUwD7. -Figure 4B Effect of CBD alone or combined which corresponds to the effective concentration 90 upon cell viability LDH release. -Figure 5A Effect of treatment with the DDS-CBD combination upon reactive oxygen species (ROS) production (Panel A) on SH SY5Y cells subjected to OGD R. -Figure 5B Effect of treatment with the DDS-CBD combination reduced glutathione levels on SH SY5Y cells subjected to OGD R. -Figure 6 Caspase-3 activity induced by OGD R on SH-SY5Y cells., Steps to reproduce: Data was analyzed using SPSS, version 25.0 software. The results of this study were performed in triplicate, with three repetitions, and were expressed as means ± SEM. Figure 1A, 1B, 2A, 2B and 3B. Viability percentage. The following formula was used to calculate cell viability: Cell viability (%) = cells in the experimental group divided by cells in the control group = 100%. LC50 and EC50 values (with 95% confidence limits) were determined by Probit regression analyses. Figure 3A, 4A, 4B, 5A, 5B and & Viability percentage. The following formula was used to calculate cell viability: Cell viability (%) = cells in the experimental group divided by cells in the control group = 100%. One Way ANOVA test., Peer reviewed

Cold plasma (CP) technology is a promising alternative to thermal treatments for the microbial decontamination of foods with low-water activity. The aim of this work is study the application of low-pressure CP (0.35 mbar) for the inactivation of Bacillus cereus in a soybean powder matrix using O₂ and synthetic air as ionizing gases. The parameters tested were an input power of 100, 200 and 300 W and an exposure time of 10 to 30 min. The excited reactive species formed were monitored by optical emission spectroscopy, and survival data were analyzed using the Weibull mathematical model. Treatments with both gases were effective in inactivating B. cereus. Air plasma resulted in a maximum 3.71-log reduction in bacterial counts at 300 W and 30 min, while O2 plasma showed the strongest inactivation ability, achieving levels higher than 5 log cycles at 300 W and > 25 min. This is likely due to the strong antimicrobial activity of oxygen-derived radicals together with carbon monoxide as an oxidation by-product. In addition, the Weibull distribution function accurately modeled the inactivation of B. cereus. Cold plasma technology is a promising approach for the decontamination of bacteria in low-water activity foods., This work was supported by MCIN/AEI/10.13039/501100011033, “ERDF A way of making Europe” and by the “European Union’’ [grants PID-2020-11631RB-C31, PID2023-149211OB-C32 and PRE-2021-098627] and by the Ministerio de Ciencia e Innovación, Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana [grant ACIF/2021/424]. The Instituto de Agroquímica y Tecnología de Alimentos-CSIC is an accredited Center of Excellence Severo Ochoa [CEX2021-001189-S], funded by MCIU/AEI / 10.13039/501100011033., With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S), Peer reviewed

[Description of methods used for collection/generation of data] Magnetisation data were collected on MPMS-XL and MPMS3 SQUID magnetometers equipped with 5 and 7 T magnets, respectively. Diamagnetic corrections were applied using Pascal's constants. Heat capacity measurements were carried out on a PPMS equipped with a 9 T magnet and a 3He cryostat, using a thin pressed pellet (ca. 1 mg) of a polycrystalline sample of 2, thermalized by ca. 0.2 mg of Apiezon N grease, whose contribution was subtracted using a phenomenological expression., [Methods for processing the data] An indirect estimation of DS and DT was performed in accordance with standard thermodynamic formulations., The solvothermal reaction of NiCl2.6H2O with 1,3,5-tri(2-hydroxyethyl)-1,3,5-triazacyclohexane, LH3, and Ln(OAc)3.4H2O in the presence of salicylaldehyde in MeOH leads to the formation of the dodecanuclear isostructural species [NiII8Ln4(L’)8(OAc)4(OH)8(H2O)4].xH2O (Ln= Dy, x=6 (1); Gd, x= 24 (2); Y, x= 10 (3); L’ = the dianion of the Schiff-base resulting from the reaction of salicylaldehyde and ethanolamine). The metallic core of the clusters describes a ring a of corner-sharing {Ni2Ln2} cubanes assembled on a central, planar rectangular {Ln4} unit. Magnetization studies conducted on complexes 1 - 3 demonstrate the presence of dominant ferromagnetic exchange at low temperatures, which is largely attributed to the Ni⋯Ni interactions. The significant magnetocaloric effect observed in the Gd analogue has been assessed through heat-capacity and magnetization measurements., This work has received support from EU (MSCA-DN MolCal, 101119865), MICIU/AEI/10.13039/501100011033/ and ERDF/EU (PID2021-124734OB-C21, CEX2023-001286-S), Gobierno de Aragón (E11-23R, E12-23R). CJM and TGT would like to thank the National Recovery and Resilience Plan Greece 2.0 (Award Number TAEDR-0535821), funded by the European Union − NextGenerationEU., With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2023-001286-S)., Peer reviewed

Esta es la versión 1 del dataset que se puede encontrar en este mismo repositorio http://hdl.handle.net/10261/410317, El conjunto de datos del proyecto The European Qur’an permite reconstruir un sistema reticular de datos curados por más de 50 investigadores. Este sistema reticular está formado por un conjunto de nodos y de relaciones entre nodos. Cada nodo se adscribe a una tipología formada por: manuscritos, textos, ediciones impresas y actores y cada nodo tiene codificado una serie de propiedades (datos) específicos descriptivos. Los nodos están enlazados por relaciones también llevan codificado su propia tipología. Aquí publicamos cuatro tablas uno por cada tipo de nodo, una tabla de relaciones entre nodos y un esquema de la base de datos. Las tablas de los nodos incluyen las siguientes propiedades: textual_composition, text_alif_maqsura_dotted, hands, writing_support, origin_of_bookbinding, language, text_hamza_colour, institution, watermark_position, quranic_reading, text_ductus_colour, number_columns, state_of_preservation, bibliographical_references, heading_ductus_colour, link_catalogue, text_alif_elongation_colour, text_vowels_colour, other_places, presence_of_numeration, number_of_lines, released_date, script, explicit, typology, text_tashid_colour, incipit, heading_alif_maqsura_dotted, colophon_alif_maqsura_dotted, colophon_calligraphic_style, headings_vocalisation, covering_material, text_calligraphic_style, first_writing_line, catchwords, headings_calligraphic_style, material_composition, folio_measures, colophon_ductus_colour, writing_baseline_orientation, text_vocalisation, colophon_vocalisation, writing_support_colour, text_sukun_colour, bookbinding_type, total_pages, writing_frame_measures, former_location, present_shelfmark, location, category, former_institution, name, city, state, year, title, original_name, education_place, birthdate, deathdate, name_variations, activity_institution, activity_start_date, main_activity, birthplace, activity_place, activity_end_date, secondary_activity, deathplace, education_institution, date, full_title, pages, quran, place_of_publication, quran_structure, title_manuscript, textual_interval, link_library_catalogue, former_shelfmark, title_position, colophon_position, attributed_title, current_location ,explicit_position, preservation_state, collection, other_dates, codicological_unit, released_place, incipit_position, Status, quran_quotations, original, formal_expression, section, source, creation_date, title_in_english, content, title_variations, content_table, genre, use_macro, use_micro, format, suras, further_notes, publication_place, author, originalname, eduaction_place, short_title, type, illustrations, colophon, quranic_structure_decoration, general_decoration, marginalia, entry_author, pshelfmark, fshelfmark, tvariations, author_entry, arabic_type, sources, printer, individuals, From, lon, lat, fol, initial_formula, origin_numeration, numeration_system, presence_numeration, headings_alif_maqsura_dotted, line_number, headings_ductus_colour, text_alif_elongation, colophon_tashid_colour, colophon_vowels_colour, colophon_sukun_colour, calligraphic_style, colour, hands_marginalia, transcription, about_the_text, position, fifth_ayats_division, surah_headings_decoration, sayda_deco_calligraphic_style, hizb_division_deco, hizb_subdivision_location, surah_headings_colour, hizb_location, hizb_subdivision_colour, sayda_decoration, sayda_location, surah_headings_remarks, hizb_decoration_colours, sayda_deco_colours, hizb_subdivision_calligraphic, tenth_ayats_division, first_ayat_division, hizb_decoration, bookbinding_measures, origin_bookbinding, decorations, decoration_colour, decoration_position, writing_area_measures, fifth_ayat_colour, numbered, fifth_ayat_markers, first_ayat_markers, tenth_ayat_markers, first_ayat_colour, dbId, sources2, rplace, stitle, quotations, atype, latitude, longitude, path La tabla de las relaciones entre nodos está compuesta por 55 tipos de relaciones: owner_of, preparatory_of, editor_of, commisioner_of, copy_of, translator_of, translation_of, author_of, protector_of, publisher_of, part_of, mentioned_in, kinship_of, copyist_of, supplement_of, supplement_to, other_relation_with, exemplum_of, printer_of, protege_of, apograph_of, reader_of, quoted, commentary_of, included_in, includes, polemic_on, quotes, uncertain_author_of, edited_in, edition_of, contribution, waqif_of, glossator_of, decorator_of, disciple_of, master_of, predecessor_of, addresse_of, same_exlibris, seller_of, transliterator_of, revisor_of, annotated_copy_of, dedicatee_of, mawquf_of, same_hand, part_of_same_manuscript, written_for, translated_in, same_shelfmark_of, borrower_of, lender_of., [EN] The European Qur’an. Islamic Scripture in European Cultures and Religion 1150-1850 (EuQu) is a research project (2019-2025 that aims to explore the ways in which the Islamic Holy Book is embedded in the intellectual, religious and cultural history of Medieval and Early Modern Europe. This research studies how the Qur’an has been translated, interpreted, adapted and used by Christians, European Jews, freethinkers, atheists and European Muslims in order to understand how the Holy Book has influenced both culture and religion in Europe. EuQu is an ERC Synergy project formed by a consortium led by the Spanish National Research Council (CSIC); the University of Naples L’Orientale (UNO); the University of Copenhagen and the University of Nantes (UN)., [ES] The European Qur’an. Islamic Scripture in European Cultures and Religion 1150-1850 (EuQu) es un proyecto de investigación (2019-2025) que tiene como objetivo explorar las formas en que el Libro Sagrado islámico está incrustado en la historia intelectual, religiosa y cultural de la Europa medieval y moderna temprana. Esta investigación estudia cómo el Corán ha sido traducido, interpretado, adaptado y utilizado por cristianos, judíos europeos, librepensadores, ateos y musulmanes europeos con el fin de comprender cómo el Libro Sagrado ha influido tanto en la cultura como en la religión en Europa. EuQu es un proyecto ERC Synergy formado por un consorcio liderado por el Consejo Superior de Investigaciones Científicas (CSIC); la Universidad de Nápoles, L'Orientale (UNO); la Universidad de Copenhague y la Universidad de Nantes (UN)., No

[Descripción de los métodos utilizados para la recopilación/generación de datos] Para evaluar el impacto del evento y analizar la percepción pública sobre investigación e investigadores/as se han tenido en cuenta indicadores tanto cualitativos como cuantitativos. Para ello cada institución ha recogido datos en las distintas actividades que se celebraron la “Noche de los Investigador@s”. Los datos fueron recogidos in situ a través de la observación y un pequeño sondeo que se entregó a los participantes en forma de tarjeta. En la tarjeta se preguntaba sobre el sexo, la edad, la forma de acceso (sólo, acompañado, etc.), la satisfacción con la actividad a la que se asiste y el aprendizaje que ha aportado al asistente. En la tarjeta también se demandaba una dirección web al objeto de enviar a los participantes un cuestionario posterior. Se ha usado un instrumento de medida en forma de cuestionario con el fin de mejorar la planificación y control del impacto general de la “Noche de los Investigador@s”. Hemos utilizado tres medios de captación de cuestionarios: - Envío de un cuestionario digitalizado a los asistentes que nos aportaron su Email a través de la tarjeta entregada en las distintas actividades. - Cuestionarios en papel entregados a los asistentes el día de celebración de la actividad. - Cuestionarios digitalizados para los asistentes que se habían inscrito en algunas actividades, cuyo envío se realizó a través de los Email aportados en las inscripciones. Además del cuestionario dirigido a los asistentes, se estimó la necesidad de construir también un cuestionario para los investigadores participantes en la Noche. Nos interesa conocer su punto de vista de cara a mejorar la planificación del evento en futuras ediciones. Por tanto, se han registrado datos procedentes de tres fuentes diferenciadas que se detallan a continuación: - Tarjeta “Cuéntanos tu Noche”. Un total de 2.252 asistentes auto cumplimentaron la tarjeta. Los datos aportados en las tarjetas nos permiten inferir el perfil de los asistentes a las distintas actividades, así como disponer de un contacto para enviar el cuestionario de evaluación. - Cuestionario de “Evaluación de Actividades”. Se han recogido 1.314 cuestionarios de los que 485 han sido captados en papel el día de la actividad, 484 a través del cuestionario digital enviado a los asistentes que se inscribieron a las distintas actividades y, finalmente, 345 cuestionarios digitales procedentes de los asistentes que nos aportaron su Email en las tarjetas entregadas el día de la actividad. - Cuestionario de “Evaluación de Investigador@s”. Un total de 232 investigador@s participantes en el evento han cumplimentado el cuestionario., [Modalidades de tratamiento de los datos] La grabación de datos es automática. En la depuración de los datos se han eliminado los casos que no habían cumplimentado el 75% del cuestionario o la tarjeta, los casos con una duración de cumplimentación inferior a 2 minutos del cuestionario y los casos cumplimentados fuera Andalucía han sido eliminados., El libro de códigos está disponible en español y en traducción al inglés., [ES] La Noche Europea de los Investigadores es un proyecto de divulgación científica en el que los investigadores/as muestran su trabajo a los ciudadanos de una forma lúdica así como su repercusión en la vida cotidiana. Se trata de una acción Marie Sklodowska Curie (MSCA), dentro del Programa Marco de Investigación e Innovación de la UE Horizonte Europa 2020. Este evento se celebra simultáneamente en más de 350 ciudades europeas de 30 países desde 2005. La evaluación de la Noche Europea de los Investigadores en Andalucía se ha realizado a través de una encuesta dirigida tanto a investigadores como al público en general. En esta edición se han recogido datos de tres fuentes diferenciadas: cuestionarios de evaluación de actividades 1.314 cuestionarios, tarjetas de ‘Cuentame tu noche’ 2.252 asistentes y cuestionarios de evaluación de los investigadores/as 232 registros., [EN] The European Researchers' Night is a scientific outreach project where researchers showcase their work to citizens in an engaging way, highlighting its impact on daily life. It is part of the Marie Sklodowska-Curie Actions (MSCA) within the EU Horizon Europe 2020 Research and Innovation Framework Programme. This event has been held simultaneously in over 350 European cities across 30 countries since 2005. The evaluation of the European Researchers' Night in Andalusia was conducted through a survey targeting both researchers and the general public. In this edition, data was collected from three distinct sources: 1.314 activity evaluation questionnaires, 2.252 "Tell me about your night" feedback cards, and 232 researcher evaluation questionnaires., Proyecto financiado por el Programa Marco de Investigación Horizon Europe de la Comisión Europea, en el marco de las acciones Marie Sktodowska-Curie., 1. Lista de archivos: - THE NIGHT 2014 Readme_EN.pdf - THE NIGHT 2014 Readme_SP.pdf A) Archivos para el dataset de Asistentes_Evaluadores: - THE NIGHT 2014 (Attendees_evaluator)_Data file.csv - THE NIGHT 2014 (Attendees_evaluator)_Data file.sav - THE NIGHT 2014 (Attendees_evaluator)_Codebook_EN.pdf - THE NIGHT 2014 (Attendees_evaluator)_Codebook_SP.pdf - THE NIGHT 2014 (Attendees_evaluator)_Questionnaire.pdf B) Archivos para el dataset de Asistentes_Perfiles: - THE NIGHT 2014 (Attendees_profile)_Data file.csv - THE NIGHT 2014 (Attendees_profile)_Data file.sav - THE NIGHT 2014 (Attendees_profile)_Codebook_EN.pdf - THE NIGHT 2014 (Attendees_profile)_Codebook_SP.pdf - THE NIGHT 2014 (Attendees_profile)_Questionnaire.pdf C) Archivos para el dataset de Investigadores - THE NIGHT 2014 (Researchers)_Data file.csv - THE NIGHT 2014 (Researchers)_Data file.sav - THE NIGHT 2014 (Researchers)_Codebook_EN.pdf - THE NIGHT 2014 (Researchers)_Codebook_SP.pdf - THE NIGHT 2014 (Researchers)_Questionnaire.pdf, Peer reviewed

Affinity study of PDA to different cationic species, viability tests on cancer and healthy cells, confocal analysis, intracellular copper levels, advanced electron microscopy characterization, western blot analysis, ROS tests, and in vivo studies., Peer reviewed

[Description of methods used for collection/generation of data] A full description of the methodology is available in the materials and methods section of the associated manuscript ("Transgenerational effects of heat shock on Drosophila melanogaster gene regulation and life history depend on adaptive environment"), with extra detail provided in the supplementary material of the manuscript. We provide a very brief summary here. Experimental design: Drosophila melanogaster from two populations (Manzanares in Spain and Akaa in Finland) were brought to the lab, and lab populations were set up, using the offspring of 10 F0 females from each population. In the F2, the critical thermal limits (CTMax) of female flies from each population were tested using heat ramping experiments. Then in the F3 we looked at heat shock responses by comparing female flies subject to a heat stress (one hour at 37 C) with unheated controls. Immediately following heat shock, female ovaries were dissected for RNA-seq and ATAC-seq analysis (pooled samples of 30 individuals, triplicated for each treatment) to investigate the effects on the transcriptome and epigenome. The bodies of flies prepared for ATAC-seq were prepared for DNA WGS reseq in order to characterise the presence of transposable elements (TEs) in these populations. We quantified the effect of heat shock on life history traits by measuring the number of eggs produced by 24 heat shocked and 24 control females from each population, the number that developed into pupae and adults, the viability of the eggs, and the time taken to pupate and eclose. These traits were measured in 4 separate F4 cohorts based on eggs laid in a 48 hour period: 0-2 days, 2-4 days, 4-6 days and 12-14 days. We then maintained 96 lines (24 females per treatment, 2 populations) for a further 2 generations, and in the F6 generation we repeated our RNA-seq and ATAC-seq experiments to see if there were any detectable transgenerational effects of heat shock (from the F3) on the transcriptomes or epigenomes of their great-grand-offspring. We also repeated the measurement of life history in the F7 generation (three generations after the previous phenotypic measurements) to check for transgenerational effects on these traits. Bioinformatics and statistics: RNA-seq data was analysed using kallisto, ATAC-seq data was analysed using the nextflow nf-core/atac pipeline, and the two data sets were integrated using the R package intepareto. Reference TEs were characterised using Tlex3, while non-reference TEs were characterised using the consensus insertions discovered by two packages: Temp2 and PoPoolationTE2. TE results were integrated using custom command line scripts. Differential expression and differential accessibility were assessed using DESEq2, with set analysis used to compare results between populations and across generations. Linear regression was used to assess the relationship between expression and accessibility in the F3 and between expression in the F3 and the F6 among key sets of genes. Chi-square tests were used to test for associations between TEs and specific patterns of expression and accessibility, and functional enrichment of GO terms was tested for various sets of genes specifically those that were i) both differentially expressed and differentially accessible in the F3; ii) DEG in the F3 and F6, and iii) associated with TEs and directional change in expression or accessibility (in most cases, sets of genes were population specific). The effect of heat shock or ancestral heat shock on phenotypic traits was tested using a variety of statistical models: linear mixed effects models (lmer) for ctmax and time to eclosion and pupation, generalised linear models (glm) with binomial error distribution for viability data, and glm with poisson error distributions for egg, pupae and adult counts. In most cases cohort was included as a random effect, so most models were run using glmer., Heat stress will increasingly affect populations, as climate change leads to higher temperatures and more extreme events such as heat waves. Adaptation is predicted to occur over long evolutionary timescales, but recent work suggests that interactions between the epigenome and transposable elements (TEs) could link environmental acclimation with rapid adaptation. However, combining all these factors in a tractable model system is difficult; consequently little is known about how these processes interact in natural genetic backgrounds to shape evolutionarily relevant phenotypes. To investigate these interactions, we carried out laboratory experiments to measure gene expression and chromatin accessibility responses to heat shock in female D. melanogaster from arid and temperate climates and their consequences for offspring phenotypes, and associated molecular responses with population variation in TEs. We then measured the same molecular and phenotypic traits three generations later to explore transgenerational inheritance. Expression and accessibility responses to heat shock varied between populations and were influenced by the presence of TEs, with more upregulated responses in the arid population. Effects of heat shock on transcription were still detectable after three generations, especially in the arid population, with many epigenetic genes transgenerationally expressed, although this was not driven by chromatin accessibility. Heat shock had negative phenotypic consequences on the initial offspring cohort, but later arid population cohorts developed quicker than controls, indicating hormesis, an effect still present in the great-great-grandoffspring. These results demonstrate the transgenerational inheritance of a potentially beneficial hormetic phenotype and associated patterns of gene expression in a natural insect population., E.H. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 101030460 (project acronym: InterChromaTE), and J.G. was supported by Grant PID2020-115874GB-I00 funded by the Spanish Ministry of Sciences and Innovation MCIN/ AEI /10.13039/501100011033 and by grant 2021 SGR 00417 funded by Departament de Recerca i Universitats, Generalitat de Catalunya. The citizen science project was supported by grant FCT-20-15710 funded by FECYT-Ministerio de Ciencia, Innovación y Universidades., File List: This dataset consists of 11 main directories. Within each folder is a seperate ##_README.txt with more information about the files and subdirectories within. Below is a brief description of the 10 directories: 01_interchromate_kallisto - 24 subdirectories, one for each RNA-seq sample. Within each are the kallisto results, including the abundance.tsv file, which is used for DESeq2 analysis. 02_interchromate_nfcoreatac -1 subdirectory containing peaks files for postions of open chromatin. The .bam files used by interpareto were too large to be included in this data deposit. 03_interchromate_intepareto - 8 Rdata objects, containing matched and integrated RNA and ATAC results for all 8 pairwise comparisons considered in the study. 04_interchromate_TEresults - 3 subdirectories, one for Tlex3 results, one for Temp2 results, and one for PoPoolationTE2 results 05_interchromate_TEconsensus - 6 text files. Four .lst files, including reference (ref) and non-reference (den) insertions for P1 (population 1 = Akaa) and P2 (population 2 - Manzanares). Two .txt files, showing TE-gene associations for each population 06_interchromate_downstream - 3 Rdata objects and 7 summary .txt tables. Output from the InterChromaTE_downstream_analysis_final_250203.R script 07_interchromate_GOenrich - 10 summary. txt tables. Output from GO enrichment analyses in the InterChromaTE_downstream_analysis_final_250203.R script 08_interchromate_phenotypic - 7 text files containing ctmax measures from the F2, counts of eggs, pupae and adults from the offspring of F3 and F6 flies, and age-to-pupation and age-to-eclosion data for these same F3 and F6 cohorts. 09_interchromate_scripts - 13 bash scripts, 3 R scripts and an R function used in the analysis of the aforementioned data 10_interchromate_reference - 10 text files (.txt/.bed/.tsv/.fasta) that provide information about the iso1 TE references or the identity, position or orientation of genes and transcripts within the reference genome. 11_interchromate_meta - 5 text files (.txt/.csv) that were used as meta data in the analysis of this dataset., Peer reviewed

Álvarez Cobelas, Miguel. Methodology of Las Madres lake study. https://doi.org/10.20350/digitalCSIC/16357 . http://hdl.handle.net/10261/360220, This dataset encompasses field data on freshwater sites sampled for environmental data and algae in Madrid County in the 2017-2022 period., Madrid Field Data (2017-2022).txt, Peer reviewed