BUSCADOR RECOLECTA

The global and local photometry and derived environmental properties for the 675 SNe presented in this analysis., DES Collaboration: Kelsey, L.; Sullivan, M.; Wiseman, P.; Armstrong, P.; Chen, R.; Brout, D.; Davis, T.M.; Dixon, M.; Frohmaier, C.; Galbany, L.; Graur, O.; Kessler, R.; Lidman, C.; Möller, A.; Popovic, B.; Rose, B.; Scolnic, D.; Smith, M.; Vincenzi, M.; Abbott, T.M.C.; Aguena, M.; Allam, S.; Alves, O.; Annis, J.; Bacon, D.; Bertin, E.; Bocquet, S.; Brooks, D.; Burke, D.L.; Rosell, A.C.; Kind, M.C.; Carretero, J.; Costanzi, M.; Da, L.N.C.; Pereira, M.E.S.; Desai, S.; Diehl, H.T.; Everett, S.; Ferrero, I.; Frieman, J.; García-Bellido, J.; Gruen, D.; Gruendl, R.A.; Gschwend, J.; Gutierrez, G.; Hinton, S.R.; Hollowood, D.L.; Honscheid, K.; James, D.J.; Mena-Fernández, J.; Miquel, R.; Rodriguez-Monroy, M.; Sanchez, E.; Sevilla-Noarbe, I., Peer reviewed

Description of the files: 2_PHASED_ALIGNMENTS: Individual phased alignments used as input to produce gene trees in IQ-TREE. In fasta format.-- 3_UNPHASED_ALIGNMENTS: Individual alignments used to produce the full ML tree. In fasta format.-- 4_CONCATENATED_ALIGNMENT: The full concatenated alignment in phylip format and a .nex file with the models and partition scheme.-- 5_DATING: Concatenated reduced alignment in phylip format and the fixed input tree in Newick format used for dating. Taxon names were renamed since MCMCtree cannot handle taxon names longer than 49 characters. The file Rename.txt can be used to rename files back to the original names using sed: sed -f Rename.txt file > renamed_file. Convergence_plots.pdf contains plots of the mean age and upper and lower 95% highest posterior density (HPD) credibility intervals of two and two MCMCtree runs against each other to assure convergence of MCMC chains.-- 6_GENETREES: Genetrees used for input in ASTRAL. In Newick format.-- 7_FINAL_TREES: The ASTRAL species tree (Fig. S2), full ML tree (Fig. S1) and reduced, dated ML tree (Fig. 1, Fig. S4), with only on specimen included per species. The dated tree is in nexus format, and the others in Newick format., Africa has undergone a progressive aridification during the last 20 My that presumably impacted organisms and fostered the evolution of life history adaptations. We test the hypothesis that shift to living in ant nests and feeding on ant brood by larvae of phyto-predaceous Lepidochrysops butterflies was an adaptive response to the aridification of Africa that facilitated the subsequent radiation of butterflies in this genus. Using anchored hybrid enrichment we constructed a time-calibrated phylogeny for Lepidochrysops and its closest, non-parasitic relatives in the Euchrysops section (Poloyommatini). We estimated ancestral areas across the phylogeny with process-based biogeographical models and diversification rates relying on time-variable and clade-heterogeneous birth-death models. The Euchrysops section originated with the emerging Miombo woodlands about 22 million years ago (Mya), and spread to drier biomes as they became available in the late Miocene. The diversification of the non-parasitic lineages decreased as aridification intensified around 10 Mya, culminating in diversity decline. In contrast, the diversification of the phyto-predaceous Lepidochrysops lineage proceeded rapidly from about 6.5 Mya when this unusual life history likely first evolved. The Miombo woodlands were the cradle for diversification of the Euchrysops section, and our findings are consistent with the hypothesis that aridification during the Miocene selected for a phyto-predaceous life history in species of Lepidochrysops, with ant nests likely providing caterpillars a safe refuge from fire and a source of food when vegetation was scarce., Peer reviewed

Supplementary Materials for the manuscript "Taxonomic composition, community structure and molecular novelty of microeukaryotes in a temperate oligomesotrophic lake as revealed by metabarcoding"., This work was supported by Grants BFU2017-90114-P from Ministerio de Economía y Competitividad (MINECO), Agencia Estatal de Investigación (AEI), and Fondo Europeo de Desarrollo Regional (FEDER) and PID2020-120609GB-I00 by MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe” by the “European Union” to IR-T. It has also received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. H2020-MSCA-ITN-2015-675752 (SINGEK Project, http://www.singek.eu/). This project has also received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant Agreement No. 949745) and the support of a fellowship from ”la Caixa” Foundation (ID 100010434) with fellowship code LCF/BQ/PI19/11690008, Peer reviewed

Supporting Information includes: Supporting Methods.-- Supporting Results.-- Figures S1 to S7.-- Tables S1, S2.-- SI References, Copyright © 2023 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND)., Peer reviewed

[Description of methods used for collection/generation of data] Data were acquired through an RGB camera mounted on a UAV at an altitude of 11m. From the images obtained by the UAV, geomatic products were systematically generated for the creation of orthomosaics. This task was performed by incorporating information extracted from the RGB channels using Agisoft PhotoScan software (Agisoft LLC, St. Louis, MO). The resulting orthomosaics were subdivided into smaller sections. To identify and label weed species, malherbology experts carried out the task manually. This involved drawing bounding boxes around each plant and annotating various visible objects present in each divided image. This process was executed using the graphical tool labelImg open source software. The final result consists of a collection of individual images, each corresponding to a specific label, i.e., to each plant of each identified species., The dataset, titled 'Unmanned Aerial Vehicle Imagery for Early Stage Weed Classification and Detection in Maize and Tomato Crops,' comprises RGB images captured from an unmanned aerial vehicle flying at an altitude of 11 meters. These images were taken during the early growing season of two summer crops: Maize (BBCH14) at the CSIC experimental farm La Poveda in Arganda del Rey, Madrid, Spain, and Tomato (BBCH501) in commercial plots located in Santa Amalia, Badajoz, Spain. The dataset includes 33,467 labels representing weeds (Atriplex patula, Chenopodium album, Convolvulus arvensis, Cyperus rotundus, Lolium rigidum, Portulaca oleracea, Salsola kali, Solanum nigrum) and crops (maize, tomato). All images are saved in *.jpg format and have been labeled using partitions extracted from an orthomosaic. The final result consists of a collection of individual images, each corresponding to a specific label, i.e., to each plant of each identified species., This work was supported by the Spanish Research State Agency (AEI) through the Project PID2020-113229RB-C41/AEI/10.13039/501100011033. The lead author, G.A. Mesías-Ruiz has been a beneficiary of a FPI fellowship by the Spanish Ministry of Education and Professional Training (PRE2018-083227)., Peer reviewed

S1 Video. Correlation between cibarial pump movement and related EPG waveform (Xi). (MP4), The meadow spittlebug Philaenus spumarius plays a key role in the transmission of the bacterium Xylella fastidiosa to olive in Apulia (South Italy). Currently, available data on P. spumarius feeding behavior is limited, and a real-time observation of the different steps involved in stylet insertion, exploratory probes, and ingestion, has never been carried out. Therefore, we performed an EPG-assisted characterization of P. spumarius female feeding behavior on olive, in order to detect and analyze the main EPG waveforms describing their amplitude, frequency, voltage level, and electrical origin of the traces during stylet penetration in plant tissues. Thereafter, each of the main waveforms was correlated with specific biological activities, through video recording and analysis of excretion by adults and excretion/secretion by nymphs. Furthermore, the specific stylet tips position within the plant tissues during each of the waveforms observed was assessed by microcomputer tomography (micro-CT). Additional EPG-recordings were carried out with males of P. spumarius on olive, in order to assess possible sex-related differences. P. spumarius feeding behavior can be described by five main distinct waveforms: C (pathway), Xc (xylem contact/pre-ingestion), Xi (xylem sap ingestion), R (resting), N (interruption within xylem phase). Compared to males, females require shorter time to begin the first probe, and their Xi phase is significantly longer. Furthermore, considering the single waveform events, males on olive exhibit longer np and R compared to females., Peer reviewed

[EN] The electrochemical behaviour of each layer deposited on CoCr surfaces was examined by electrochemical impedance spectroscopy (EIS) for 7 days in 3 g/L hyaluronic acid (HA) aqueous solution. Impedance tests were performed in a three-electrode electrochemical cell with the modified CoCr surfaces acting as the working electrode, a Pt wire acting as counter electrode, and Ag/AgCl (3 M KCl) as a reference electrode. The testing area of the working electrode for all electrochemical tests was 0.39 cm2. An Autolab32 potentiostat/galvanostat was used to generate a sinusoidal wave of 10 mV amplitude in the frequency range 100 kHz to 1 mHz, logarithmically spaced by 5 points/decade, which was applied at the corrosion potential Ecorr. Nova software was used for the data acquisition. All the tests were performed in duplicate. The dataset includes only the experimental data used in the paper. The following files for each surface modification and each immersion time are included: CoCr, CoCr-OH, CoCr-OH-Si45, CoCr-OH-Si45-GO12h, CoCr-OH-Si45-GO24h, CoCr-OH-Si75, CoCr-OH-Si75-GO12h, CoCr-OH-Si75-GO24h. Each file is composed of 5 columns: frequency, real impedance (Z'), imaginary impedance (Z''), Impedance modulus (Z) and phase angle (Phase). All files are in open extension such as .txt and are protected by a Creative Commons Attribution 4.0 International (CC BY 4.0) licence. [ES] El comportamiento electroquímico de cada capa depositada en superficies de CoCr fue examinado mediante espectroscopía de impedancia electroquímica (EIS) durante 7 días sumergidas en una solución acuosa de ácido hialurónico (HA) al 3 g/L. Las pruebas de impedancia se realizaron en una celda electroquímica de tres electrodos con las superficies modificadas de CoCr actuando como electrodo de trabajo, un alambre de Pt actuando como electrodo auxiliar y Ag/AgCl (3 M KCl) como electrodo de referencia. El área de ensayo del electrodo de trabajo para todas las pruebas electroquímicas fue de 0.39 cm2. Se utilizó un potenciostato/galvanostato Autolab32 para generar una onda sinusoidal de amplitud de 10 mV en el rango de frecuencia de 100 kHz a 1 mHz, espaciada logarítmicamente por 5 puntos/década, que se aplicó en el potencial de corrosión Ecorr. Se utilizó el software Nova para la adquisición de datos. Todas las pruebas se realizaron por duplicado. El conjunto de datos incluye solo los datos experimentales utilizados en el artículo. Los siguientes archivos para cada modificación de superficie y cada tiempo de inmersión están incluidos: CoCr, CoCr-OH, CoCr-OH-Si45, CoCr-OH-Si45-GO12h, CoCr-OH-Si45-GO24h, CoCr-OH-Si75, CoCr-OH-Si75-GO12h, CoCr-OH-Si75-GO24h. Cada archivo está compuesto por 5 columnas: frecuencia, impedancia real (Z'), impedancia imaginaria (Z''), módulo de impedancia (Z) y ángulo de fase (Fase). Todos los archivos están en extensión abierta como .txt y están protegidos por una licencia Creative Commons Attribution 4.0 International (CC BY 4.0)., [EN] This is the experimental dataset used in the paper “Barrier Graphene Oxide on a CoCr Alloy via Silane/GO Covalent Bonding and Its Electrochemical Behavior in a Simulated Synovial Fluid Electrolyte". The article includes the experimental procedure carried out for the deposition of graphene oxide on CoCr surfaces: CoCr was hydroxylated through alkalization in NaOH 5 M for 2 h, (sample named CoCr-OH). Subsequently, silanization was carried out using APTES. APTES was pre-mixed at 2% vol in isopropanol-water (200:1 v/v) and stirred for 1 h. After this, CoCr-OH samples were immersed in the APTES solution at room temperature for 1 min. The silane-coated samples were kept under curing conditions at 45 °C (CoCr-OH-Si45 sample) and 75 °C (CoCr-OH-Si75 sample) for 24 h and 30 min, respectively. Finally, CoCr-OH-Si45 and CoCr-OH-Si75 surfaces were immersed in 4 g/L GO aqueous suspension at 60 °C for 12 h and 24 h to obtain CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h and CoCr-OH-Si45-GO24h, and CoCr-OH-Si75-GO24h. This data set includes the results of the electrochemical impedance data obtained for each CoCr surface: CoCr (CoCr, CoCr-OH, CoCr-OHSi45, CoCr-OH-Si75 CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h, immersed in 3 g/L Hyaluronic acid solution for 7 days., [ES] Este es el conjunto de datos experimentales utilizado en el artículo "Barrera de Óxido de Grafeno en una Aleación CoCr mediante enlace covalente de Silano/GO y su comportamiento electroquímico en un electrolito de fluido sinovial simulado". El artículo incluye el procedimiento experimental llevado a cabo para la deposición de óxido de grafeno sobre superficies de CoCr: CoCr fue hidroxilado mediante alcalinización en NaOH 5 M durante 2 horas (muestra denominada CoCr-OH). Posteriormente, se llevó a cabo la silanización utilizando APTES. APTES fue premezclado al 2% en volumen en isopropanol-agua (200:1 v/v) y agitado durante 1 hora. Después, las muestras de CoCr-OH fueron sumergidas en la solución de APTES a temperatura ambiente durante 1 minuto. Las muestras recubiertas con silano se mantuvieron bajo condiciones de curado a 45 °C (muestra CoCr-OH-Si45) y 75 °C (muestra CoCr-OH-Si75) durante 24 horas y 30 minutos, respectivamente. Finalmente, las superficies CoCr-OH-Si45 y CoCr-OH-Si75 fueron sumergidas en suspensión acuosa de 4 g/L GO a 60 °C durante 12 y 24 horas obteniendo las superficies CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h. Este conjunto de datos incluye los resultados de los datos de impedancia electroquímica obtenidos para cada superficie de CoCr (CoCr, CoCr-OH, CoCr-OHSi45, CoCr-OH-Si75 CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h sumergidas en una solución de 3 g/L ácido hialurónico durante 7 días., We acknowledge financial support from the Spanish Ministerio de Ciencia, Innovación y Univeesidades, “ERDF A way of making Europe” and “ESF Investing in your future” through projects RTI2018-101506-B-C31 and RTI2018-101506-B-C33, and PRE2019-090122., Peer reviewed

S2 Video. Video obtained from micro-CT showing P. spumarius stylets in xylem vessel. (MKV), The meadow spittlebug Philaenus spumarius plays a key role in the transmission of the bacterium Xylella fastidiosa to olive in Apulia (South Italy). Currently, available data on P. spumarius feeding behavior is limited, and a real-time observation of the different steps involved in stylet insertion, exploratory probes, and ingestion, has never been carried out. Therefore, we performed an EPG-assisted characterization of P. spumarius female feeding behavior on olive, in order to detect and analyze the main EPG waveforms describing their amplitude, frequency, voltage level, and electrical origin of the traces during stylet penetration in plant tissues. Thereafter, each of the main waveforms was correlated with specific biological activities, through video recording and analysis of excretion by adults and excretion/secretion by nymphs. Furthermore, the specific stylet tips position within the plant tissues during each of the waveforms observed was assessed by microcomputer tomography (micro-CT). Additional EPG-recordings were carried out with males of P. spumarius on olive, in order to assess possible sex-related differences. P. spumarius feeding behavior can be described by five main distinct waveforms: C (pathway), Xc (xylem contact/pre-ingestion), Xi (xylem sap ingestion), R (resting), N (interruption within xylem phase). Compared to males, females require shorter time to begin the first probe, and their Xi phase is significantly longer. Furthermore, considering the single waveform events, males on olive exhibit longer np and R compared to females., Peer reviewed

This study presents an exceptional collection of 54 Late Pleistocene human remains that correspond to at least three Neanderthal individuals from Simanya Gran, the main gallery of Cova Simanya, located in the northeastern Iberian Peninsula. The collection comprised 53 unpublished remains that were unearthed during the 1970s and an additional tooth discovered during 2021 excavations. The specimens represent an adult with a small stature, a periadolescent aged approximately 11.5 years, and an immature individual aged approximately 7.7 years, thus offering a more complete demographic perspective. The collection encompasses diverse anatomical parts including upper and lower dentition, mandible, vertebrae, and limb bones from both the upper and lower extremities. Attempts to extract aDNA were unsuccessful. Renewed archaeological investigations at Cova Simanya have facilitated the reevaluation of the original stratigraphic context of these remains, leading to the discovery of the additional tooth, aligning with the periadolescent individual. This assemblage is currently the most extensive Neanderthal collection from the northeastern Mediterranean Iberia, offering invaluable insights into the morphology and evolutionary trajectory of Late Pleistocene hominins. Hence, Simanya Neanderthals will enhance our understanding of Neanderthal demographics and evolution, paving the way for an in-depth examination of the morphological diversity and evolutionary context of Iberian Neanderthals., Peer reviewed

© 2023 The Authors. Global Change Biology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited., This supplementary appendix contains: 1. Supplementary Methods.-- 2. Supplementary Figures.-- 3. Supplementary References, Peer reviewed