Resultados de investigación

Example for the determination of the enyzmatic activity of CalB, self-degradation results for PBS_10% films, visual aspect of degraded films, SEM images of the cross-section of PBAT_5% and PBS_5% films, FTIR spectra of nondegraded and degraded PLA films, molecular weight distribution through GPC for the blank experiments, FTIR spectra of nondegraded and degraded films obtained from the three blends, and changes in the degree of crystallinity of the degraded samples from the three blends., Peer reviewed

[High-Performance Liquid Chromatography (HPLC) of Fmoc-FFpY]; [Mass spectrometry of Fmoc-FFpY], Peer reviewed

Dataset from experimets developed in the article "FUNCTIONAL DISTANCE MEDIATES PLANT INTERACTIONS". The outcome of plant interactions is strongly dependent on the set of physiological, morphological, and reproductive traits harboured by the interacting species. Two trait-based mechanisms are behind these plant interactions: a) trait dissimilarities, when species with similar traits compete more due to niche overlap, and b) trait hierarchies, when species with more efficient traits outcompete species with inefficient traits. Trait dissimilarities present a negative relationship between plant functional distance and interaction strength, and a positive relationship for trait hierarchies. We hypothesised that functional distance between species affect the outcome of plant interactions through both mechanisms. We established a manipulative experiment with ten species growing in hetero- and conspecific pairwise combinations and evaluated thirteen above- and belowground traits. We estimated neighbour effect by measuring the relative change (RC) in trait values of a focal species (RCfocal) caused by the presence of a heterospecific neighbour (RCneighbour), contrasted with the changes induced by a conspecific neighbour. We explored i) which traits were more affected by neighbours, ii) which species were more affected as focal or neighbour, and iii) how the neighbour effect varied with the functional distance between pairs. Neighbour effects on focal traits showed a variable intensity, contingent upon the specific identities of both plant species within the pair. Despite the high variability in neighbour effects, species that grew with a functionally similar neighbour exhibited larger traits than those growing with a dissimilar one due to the prevalence of trait hierarchies over trait dissimilarities. The heterogeneity found on the neighbour effects responded mainly to differences between above- and belowground traits, reinforcing the need to consider them both for a mechanistic comprehension of community dynamics. Trait hierarchies prevailed but trait dissimilarities were also detected on specific root traits, indicating that both mechanisms simultaneously determine the outcome of plant interactions., Peer reviewed

[Figure S1. Lamp spectrum]; [Figure S2. FTIR spectra of the material extracted (EXT) from PP using CH2Cl2. The spectrum of PP is included as reference.]; [Figure S3. DLS size distribution of 50 mg/L PPd-NPs in D. magna freshwater medium.] [Table S1. Fitting parameters b and EC10 (± standard deviation), mg/L.], Peer reviewed

[S1. Recipes of the reinforced ionic elastomers (in phr).] [S2. Curing curves of the reinforced ionic elastomers.] [S3. DSC and TGA curves of the reinforced ionic elastomers.] [S4. Stress-strain curves of the reinforced ionic elastomers.], Peer reviewed

Cytochrome-c oxidase (COX) is part of the mitochondrial complex IV (CIV). COX deficiency is usually associated with tRNA variants, and less frequently with variants in COX assembly factors. Mutations in COX subunits encoded by mitochondrial DNA and nuclear DNA are rare, likely because most of them are associated to very severe phenotypes with early lethality. COX18, an assembly factor of CIV, has long been analyzed as a potential cause of mitochondrial disease. To date, only one patient has been identified carrying a homozygous missense variant in COX18, associated with neonatal encephalo-cardiomyopathy and axonal sensory neuropathy. Here, we describe a 40-year-old patient, asymptomatic until 7 months of age, who presented with progressive muscle weakness resembling spinal muscle atrophy type-2, associated with oculofacial apraxia and dysarthric speech. Electrophysiology analysis highlighted a severe sensory-motor neuropathy. Muscle biopsy showed striking and diffuse decreases of COX staining and a substantial reduction of CIV activity. Muscle biopsy showed no ragged-red fibers, although ultrastructural mitochondrial alterations were evident. A novel homozygous variant (c.598G>A), located in the last nucleotide of exon 3, was detected in COX18 by whole-exome sequencing, which affected the splicing donor site, as demonstrated by cDNA-seq. The patient fibroblasts express a truncated form of COX18 (COX18Δ112-240) capable of assembling CIV and CIV-involving supercomplexes. However, CIV activity was decreased. COX18 full-length (COX18-fl) overexpression partially rescued CIV activity in the patient fibroblasts. The rescue of the null CIV activity in COX18-KO-HEK293 cells by overexpressing of COX18Δ112-240 was significantly lower than in cells with COX18-fl. In addition, cox-18 downregulation in C. elegans resulted in slow growth and, diminished reduced motility phenotypes and as well as severe fragmentation of the mitochondrial network. Our case expands the phenotypes associated with COX18 variants and supports the pathogenic role of COX18 as the cause of a severe encephaloneuropathy syndrome, The authors acknowledge the patient and his family, as well as the patient association ASENSE for all the support to this work. We also acknowledge the facilities and scientific and technical assistance of CITIUS (University of Seville) and the Biomolecular Mass Spectrometry Service of the Pablo de Olavide University. This work was supported in part by the Instituto de Salud Carlos III (FIS PI19-01497 to C. Paradas), Consejería de Salud y Familia, Junta de Andalucía and Programa Operativo Fondo Social Europeo de Andalucía 2014–2020 (RH0046-2020 to G.C) and Consejería de Salud y Consumo, Junta de Andalucía and Programa Nicolas Monarde (C1-0003-2022 to E.S-M). We also acknowledge the FP7 WeNMR (project# 261572), H2020 West-Life (project# 675858), the EOSC-hub (project# 777536) and the EGI-ACE (project# 101017567) European e-Infrastructure projects for the use of their web portals, which make use of the EGI infrastructure with the dedicated support of CESNET-MCC, INFN-LNL-2, NCG-INGRID-PT, TW-NCHC, CESGA, IFCA-LCG2, UA-BITP, TR-FC1-ULAKBIM, CSTCLOUD-EGI, IN2P3-CPPM, CIRMMP, SURFsara and NIKHEF, and the additional support of the national GRID Initiatives of Belgium, France, Italy, Germany, the Netherlands, Poland, Portugal, Spain, UK, Taiwan and the US Open Science Grid. This work was supported by the Spanish Government (PGC2018-096049-B-I00, FEDER/Ministerio de Ciencia e Innovación — Agencia Estatal de Investigación Grant PID2021-126663NB-I00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe), European Regional Development Fund (FEDER), Andalusian Government (BIO-198, US/JUNTA/FEDER) and the Ramón Areces Foundation (2021–2024 to I.D.-M.). Some of the authors are part of the European Research Network in Neuromuscular Diseases (EURO-NMD)., Peer reviewed

Financial support was provided by the Andalusian Ministry of Economy, Science and Innovation in the framework of the Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020, programme 54ª “Investigación científica e innovación”, “POSTDOC_21_00594”), the Spanish Ministry of Science and Innovation (Ref: PID2020-119949RB-I00), the Andalusian Ministry of Economy, Science and Innovation cofinanced by the European Regional Development Fund (ERDF) from FEDER and the European Social Fund (ESF) (PY20_00882 and CV20-04221). The COST action CA-18132 “Functional Glyconanomaterials for the Development of Diagnostic and Targeted Therapeutic Probe” is also acknowledged. The COST action CA-22147 “European metal–organic framework network: combining research and development to promote technological solutions” (EU4MOFs) is also acknowledged., Peer reviewed

This work introduces NeoMag, a system designed to enhance cell mechanics assays in substrate deformation studies. NeoMag uses multidomain magneto-active materials to mechanically actuate the substrate, transmitting reversible mechanical cues to cells. The system boasts full flexibility in alternating loading substrate deformation modes, seamlessly adapting to both upright and inverted microscopes. The multidomain substrates facilitate mechanobiology assays on 2D and 3D cultures. The integration of the system with nanoindenters allows for precise evaluation of cellular mechanical properties under varying substrate deformation modes. The system is used to study the impact of substrate deformation on astrocytes, simulating mechanical conditions akin to traumatic brain injury and ischemic stroke. The results reveal local heterogeneous changes in astrocyte stiffness, influenced by the orientation of subcellular regions relative to substrate strain. These stiffness variations, exceeding 50% in stiffening and softening, and local deformations significantly alter calcium dynamics. Furthermore, sustained deformations induce actin network reorganization and activate Piezo1 channels, leading to an initial increase followed by a long-term inhibition of calcium events. Conversely, fast and dynamic deformations transiently activate Piezo1 channels and disrupt the actin network, causing long-term cell softening. These findings unveil mechanical and functional alterations in astrocytes during substrate deformation, illustrating the multiple opportunities this technology offers., C. G.-C. and M. F.-d.l.T. authors contributed equally to this work. The authors thank Lotte van Dasler for the relevant discussion. The authors acknowledged support from the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation Program (Grant agreement no. 947723, project: 4D-BIOMAP and Grant agreement no. 101081713, project: ISBIOMECH). G.P. acknowledged support from PID2019-106579RB-I00 funded by MCIN/AEI/10.13039/5011 00011033 and by “ERDF A way of making Europe”, and La Marató TV3 Foundation #225619. AMB acknowledged support from Ministerio de Ciencia, Innovación y Universidades, Agencia Estatal de Investigación Grant PID2019-109820RB-I00, MCIN/AEI/10.13039/501100011033/, co-financed by the European Regional Development Fund (ERDF), ‘A way of making Europe’. C.G.-C. acknowledged support from the Ministerio de Universidades, Spain (FPU20/01459) and A.M.B. from Universidad Carlos III de Madrid ('Ayudas para la recualificación del profesorado funcionario o contratado')., Peer reviewed

Peer reviewed

A) Gene ontology analysis with gProfiler for genes in cluster A. B) Similar analysis for genes in cluster D. C) Distribution of identified clusters and logistic regression analyses within the RIBI regulon and subcategories (upper section) and within the RP regulon and subcategories (lower section). D) Representation of total RNAP II (RNAp ChIP) and active RNAP II (GRO) in RiBi and RP genes. mRNA expression and mRNA decay data from Eser et al. [17] are also represented. E) Normalized values for all genes (grey), RiBi regulon (red), and RP regulon (green) for total RNAP II (RNAp ChIP) and active RNA Pol II (GRO). The proportion of active polymerases was calculated as described in Fig 1B., Peer reviewed