Buscador | Buscador

Background: Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors of internationally important arboviruses. To understand the role of Culicoides in the transmission of these viruses, it is essential to correctly identify the species involved. Within the western Palaearctic region, the main suspected vector species, C. obsoletus, C. scoticus, C. dewulfi and C. chiopterus, have similar wing patterns, which makes it difficult to separate and identify them correctly. Methods: In this study, designed as an inter-laboratory ring trial with twelve partners from Europe and North Africa, we assess four PCR-based assays which are used routinely to differentiate the four species of Culicoides listed above. The assays based on mitochondrial or ribosomal DNA or microarray hybridisation were tested using aliquots of Culicoides DNA (extracted using commercial kits), crude lysates of ground specimens and whole Culicoides (265 individuals), and non-Culicoides Ceratopogonidae (13 individuals) collected from across Europe. Results: A total of 800 molecular assays were implemented. The in-house assays functioned effectively, although specificity and sensitivity varied according to the molecular marker and DNA extraction method used. The Obsoletus group specificity was overall high (95-99%) while the sensitivity varied greatly (59.6-100%). DNA extraction methods impacted the sensitivity of the assays as well as the type of sample used as template for the DNA extraction. Conclusions: The results are discussed in terms of current use of species diagnostic assays and the future development of molecular tools for the rapid differentiation of cryptic Culicoides species., Published

Proyecto:

Ver en: http://hdl.handle.net/10532/2579

Evaluación comparativa de la alfalfa y la esparceta en condiciones de secano y regadío de Aragón, Comparative evaluation of lucerne and sainfoin in rainfed conditions and under irrigation in Aragon

Delgado Enguita, Ignacio
Muñoz Pérez, Fernando
Andueza Urra, Jesús Donato

Published

Proyecto:

Ver en: http://hdl.handle.net/10532/2580

Pautas de aprovechamiento espacio-temporal de los pastos por el ganado en una estación de esquí del Pirineo Oscense: recomendaciones para optimizar el uso de los recursos, Patterns of pasture use by cattle in a Pyrenean ski station : recommendations for optimal use of resources

Casasús Pueyo, Isabel
Rodríguez Sánchez, José Antonio
Sanz Pascua, Albina
Ferrer Benimeli, Carlos
Reiné Viñales, Ramón
Barrantes Díaz, Olivia

Published

Proyecto:

Ver en: http://hdl.handle.net/10532/2581

Validación de sistemas automáticos de control de la ingestión de forraje y concentrado en ganado vacuno, Validation of two automatic systems for monitoring forage and concentrate intake of beef cows

Villalba Mata, Daniel
Cortés Lacruz, X.
Blanco Alibés, Mireia
Ferrer Achiaga, Javier
Casasús Pueyo, Isabel

Published

Proyecto:

Ver en: http://hdl.handle.net/10532/2582

Draft genome sequence of Xanthomonas arboricola pv. pruni strain Xap33, causal agent of bacterial spot disease on almond

Garita Cambronero, J.
Sena Vélez, M.
Palacio Bielsa, Ana
Cubero, J.

We report the annotated genome sequence of Xanthomonas arboricola pv. pruni strain Xap33, isolated from almond leaves showing bacterial spot disease symptoms in Spain. The availability of this genome sequence will aid our understanding of the infection mechanism of this bacterium as well as its relationship to other species of the same genus., Published

Proyecto:

Ver en: http://hdl.handle.net/10532/2583

Buscador avanzado