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Abiotic factors modulate post-drought growth resilience of Scots pine plantations and rear-edge Scots pine and oak forests

  • Rubio-Cuadrado, Á and Camarero, J. J.
  • Aspizua, R.
  • Sánchez-González, M.
  • Gil, L.
  • Montes, F.
Proyecto:


Physio-metabolic response of rainbow trout during prolonged food deprivation before slaughter

  • Bermejo-Poza, R.
  • Fernández-Muela, M.
  • De la Fuente, J.
  • Pérez, C.
  • González de Chavarri, E.
  • Díaz, M. T.
  • Torrent, F.
  • Villarroel, M.
Proyecto:


Plasma proteomic analysis of zebrafish following spring viremia of carp virus infection

  • Medina-Gali, R.
  • Belló-Pérez, M.
  • Ciordia, S.
  • Mena, M. C.
  • Coll, J.
  • Novoa, B.
  • Ortega-Villaizán, M. D. M.
  • Perez, L.
Proyecto:


Racial verification of Iberian ham and shoulders commercialized in Spanish supermarkets

  • Caraballo, C.
  • Muñoz, M.
  • Rodríguez, C.
  • Silió, L.
  • García-Casco, J. M.
Proyecto:


Nucleotide sequence and variability of the inverted terminal repetitions of African swine fever virus DNA

  • de la Vega, I.
  • González, A.
  • Blasco, R.
  • Calvo, V.
  • Viñuela, E.
African Swine fever virus (ASFV) genome is a large (170-190 kb) double-stranded DNA molecule with structural features similar to those of Poxviruses. Prominent among those features are the presence of a hairpin loop structure at the end of the DNA molecule and terminal-inverted repeats (TIR). The TIRs have been previously demonstrated by electron microscopy and cross-hybridization of terminal restriction fragments. We have determined the sequence of both left and right DNA ends from the BA71V virus strain. The TIR is composed of 2134 bp and was identical at both genome ends. As much as 82% of the TIR sequence is made up of short (27-35 bp) sequences repeated within the TIR, in five different sets of repeats. The central portion of the TIR is occupied by a 34-bp sequence which is repeated in tandem 33 times. Length differences were found within TIRs of virus clones isolated from a single infected animal. The ASFV TIR resembles in structure the TIR of the related Poxviruses. © 1994 Academic Press. All rights reserved.
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Transcriptional profiles of arabidopsis stomataless mutants reveal developmental and physiological features of life in the absence of stomata

  • de Marcos, A.
  • Triviño, M.
  • Pérez-Bueno, M. L.
  • Ballesteros, I.
  • Barón, M.
  • Mena, M.
  • Fenoll, C.
Loss of function of the positive stomata development regulators SPCH or MUTE in Arabidopsis thaliana renders stomataless plants; spch-3 and mute-3 mutants are extreme dwarfs, but produce cotyledons and tiny leaves, providing a system to interrogate plant life in the absence of stomata. To this end, we compared their cotyledon transcriptomes with that of wild-type plants. K-means clustering of differentially expressed genes generated four clusters clusters 1 and 2 grouped genes commonly regulated in the mutants, while clusters 3 and 4 contained genes distinctively regulated in mute-3. Classification in functional categories and metabolic pathways of genes in clusters 1 and 2 suggested that both mutants had depressed secondary, nitrogen and sulfur metabolisms, while only a few photosynthesis-related genes were down-regulated. In situ quenching analysis of chlorophyll fluorescence revealed limited inhibition of photosynthesis. This and other fluorescence measurements matched the mutant transcriptomic features. Differential transcriptomes of both mutants were enriched in growth-related genes, including known stomata development regulators, which paralleled their epidermal phenotypes. Analysis of cluster 3 was not informative for developmental aspects of mute-3. Cluster 4 comprised genes differentially up-regulated in mute-3, 35% of which were direct targets for SPCH and may relate to the unique cell types of mute-3. A screen of T-DNA insertion lines in genes differentially expressed in the mutants identified a gene putatively involved in stomata development. A collection of lines for conditional over expression of transcription factors differentially expressed in the mutants rendered distinct epidermal phenotypes, suggesting that these proteins may be novel stomatal development regulators. Thus, our transcriptome analysis represents a useful source of new genes for the study of stomata development and for characterizing physiology and growth in the absence of stomata. © 2015 de Marcos
Proyecto:


Evidence of intense chromosomal shuffling during conifer evolution

  • de Miguel, M.
  • Bartholomé, J.
  • Ehrenmann, F.
  • Murat, F.
  • Moriguchi, Y.
  • Uchiyama, K.
  • Ueno, S.
  • Tsumura, Y.
  • Lagraulet, H.
  • De Maria, N.
  • Cabezas, J. A.
  • Cervera, M. T.
  • Gion, J. M.
  • Salse, J.
  • Plomion, C.
Although recent advances have been gained on genome evolution in angiosperm lineages, virtually nothing is known about karyotype evolution in the other group of seed plants, the gymnosperms. Here, we used high-density gene-based linkagemapping to compare the karyotype structure of two families of conifers (the most abundant group of gymnosperms) separated around 290 Ma Pinaceae and Cupressaceae.We propose for the first time amodel based on the fusion of 20 ancestral chromosomal blocks that may have shaped the modern karyotpes of Pinaceae (with n=12) and Cupressaceae (with n=11). The considerable difference in modern genome organization between these two lineages contrasts strongly with the remarkable level of synteny already reported within the Pinaceae. It also suggests a convergent evolutionary mechanism of chromosomal block shuffling that has shaped the genomes of the spermatophytes. ©The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
Proyecto:


Immunization with DNA vaccines containing porcine reproductive and respiratory syndrome virus open reading frames 5, 6, and 7 may be related to the exacerbation of clinical disease after an experimental challenge

  • Díaz, I.
  • Ganges, L.
  • Galindo-Cardiel, I.
  • Tarradas, J.
  • Álvarez, B.
  • Lorca-Oró, C.
  • Pujols, J.
  • Gimeno, M.
  • Darwich, L.
  • Domingo, M.
  • Domínguez, J.
  • Mateu, E.
Pigs were immunized with DNA plasmids containing different open reading frames (ORFs) of a porcine reproductive and respiratory syndrome virus (PRRSV) genotype I strain. One group was injected with three inoculations of ORF7, a second group was immunized with three inoculations of plasmids containing ORF5 and ORF6, and a third group was kept as controls. Later, +21 days after the last inoculation, animals were challenged with the homologous strain. After the challenge, PRRSV-specific interferon (IFN)-γ-secreting cells and anti-PRRSV IgG antibodies developed faster in DNA vaccinated pigs (p<0.05). However, DNA-immunized pigs showed an exacerbation of the disease compared to the unvaccinated challenged pigs. The data suggest that previous immunization with DNA vaccines against glycoprotein 5 and/or matrix protein of PRRSV, as well as nucleoprotein but to a lesser degree, could result in an exacerbation of the clinical course in terms of fever upon challenge. © Mary Ann Liebert, Inc. 2013.
Proyecto:


Use of concentrate or pasture for fattening lambs and its effect on carcass and meat quality

  • Díaz, M. T.
  • Velasco, S.
  • Cañeque, V.
  • Lauzurica, S.
  • Ruiz De Huidobro, F.
  • Pérez, C.
  • González, J.
  • Manzanares, C.
Two production systems, pasture and sheepfold based on lambs fed concentrates ad libitum was evaluated for carcass and meat quality of lambs slaughtered at body weights of 24 and 28 kg. There was no effect of production system on growth rate of lambs. In sheepfold, commercial carcass dressing values of slaughter lambs were heavier than in those raised at pasture with more dorsal fat thickness (1.6 mm vs. 1.1 mm), kidney knob and channel fat (1.6% vs. 1.2%) and a greater percentage of leg fat (8.5% vs. 6.6%). Lambs at heavier weights had more fat. Meat quality, pH, water-holding capacity and collagen content parameters were not affected by the production systems. The color of the longissimus dorsi muscle was somewhat darker in lambs fattened at pasture, but no color differences were seen in the rectus abdominis muscle. In both subcutaneous and intramuscular fat of the two cuts studied (leg and loin), sheepfold lambs presented a lower percentage of stearic acid (C180) and a higher proportion of palmitic (C160) and linoleic (C182) acids, and thus a lower percentage of SFA and a higher one of TUFA, while a better (n - 6)/(n - 3) ratio was observed in lambs raised at pasture. Lambs at heavier weights displayed a lower percentage of PUFA and PUFA/SFA ratio in the intramuscular fat of the longissimus dorsi muscle and higher levels of oleic (C181) and palmitic (C160) fatty acids and a lower one of myristic acid (C140) in both depots. © 2002 Elsevier Science B.V. All rights reserved.
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