BUSCADOR RECOLECTA

21 páginas, 16 figuras, Peer reviewed

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The pharyngeal gene architectures are shown for presumed last common ancestors at key phylogenetic nodes (a) and selected living metazoan species (b) (see S26 Fig for the complete dataset). Genes that are commonly linked together are shown in the same color; homeobox-containing genes, including nkx2.1, nkx2.2 and msx, are in green, mipol1 and foxa genes are in blue, and pax1/9 and slc25a21 are in red. The gray circles indicate genes that are located within the pharyngeal gene cluster. Double slashes are introduced when more than 3 genes are located in between 2 pharyngeal genes. Because pax genes of cnidarians and sponges do not show one-to-one correspondence with those of bilaterians, we surveyed the locations of all potential pax genes and found that none is linked with the other pharyngeal-related genes in cnidarians and sponges., Peer reviewed

GO enrichment analysis of genes located in the sea star POC9 (a), sea urchin SPU1 (b), hemichordate PFL18 (c), and PFL9 (d). The echinoderm chromosomes (POC12 and SPU3) corresponding to deuterostome DALG R were also analyzed to understand the chordate-specific chromosomal dispersion (e). The enriched GO terms (adjusted p-value <0.1) are clustered and divided into different modules, and selected terms are underlined. The top 3 enriched BP GO terms of each module are shown in S17–S19 Figs. The data underlying this figure can be found in S2, S3, and S4 Data. BP, biological process; GO, gene ontology., Peer reviewed

(a) Densities of all TEs (DNA + LTR + LINE + SINE) within the Hox cluster region and non-Hox region of the Hox-bearing chromosome/scaffold of each species. The percent differences in normalized TEs counts between the non-Hox region and the Hox cluster region are illustrated (dashed bars and red values). (b) Distributions of all TEs around the Hox gene cluster of each species. The bin size for each histogram is 10,000 bp. Dotted lines indicate the averaged TE densities of the Hox-bearing chromosomes/scaffolds. Color coding denotes division of Hox genes in “anterior” (dark blue), “group 3” (yellow), “middle” (green), and “posterior” (red) groups. The light blue crosses represent missing Hox genes. Double arrows in light blue indicate inversion events of Hox genes. The data underlying this figure can be found in S1 Data. LINE, long interspersed nuclear elements; LTR, long terminal repeats; SINE, short interspersed nuclear elements; TE, transposable element., Peer reviewed

(a, b) A schematic representation of chromosome evolution in deuterostome lineages. The chromosomal architectures of presumed LCAs (bottom in a and left in b) and the chromosomal architectures of living deuterostome species (top in a and right in b). Each box denotes an individual chromosome. Haploid number (1N) and increase (+) or decrease (-) in quantity of chromosomes are indicated. The color code of boxes is taken from the previous study on vertebrate ancestral chromosomes, except for the 9 one-to-one corresponding chromosomes (a, light gray boxes). Chromosomal architecture of the LCA of vertebrates was based on the previous study [20]. In cases where chromosomal fusion events were deduced, types of changes are indicated below color boxes with symbols defined previously [19]; end-end translocation (●), centric insertion (↘), and fusion-with-mixing (⊗). Box sizes do not reflect the actual sizes of chromosomes. (c) Chromosomal positions of the orthologous gene pairs among 5 deuterostome species. Horizontal bars with numbers on top represent chromosomes of each species. In total, 3,668 orthologous gene pairs are illustrated. For ease of comparison, the chromosome sizes are scaled proportionally such that the 5 genome assemblies reach equal sizes. Except for the genes that spread into multiple chromosomes in amphioxus (BFL), gene pairs that are not located on the corresponding chromosomal pairs or cannot be found in all 5 species are not shown. The data underlying this figure can be found in S1 Data. BFL, Branchiostoma floridae; LCA, last common ancestor., Peer reviewed

A custom Python script for calculating Fisher’s exact test., Peer reviewed

The target of rapamycin (TOR) signalling pathway plays a key role in the coordination between cellular growth and the cell cycle machinery in eukaryotes. The underlying molecular mechanisms by which TOR might regulate events after anaphase remain unknown. We show for the first time that one of the 2 TOR complexes in budding yeast, TORC1, blocks the separation of cells following cytokinesis by phosphorylation of a member of the NDR (nuclear Dbf2-related) protein-kinase family, the protein Cbk1. We observe that TORC1 alters the phosphorylation pattern of Cbk1 and we identify a residue within Cbk1 activation loop, T574, for which a phosphomimetic substitution makes Cbk1 catalytically inactive and, indeed, reproduces TORC1 control over cell separation. In addition, we identify the exocyst component Sec3 as a key substrate of Cbk1, since Sec3 activates the SNARE complex to promote membrane fusion. TORC1 activity ultimately compromises the interaction between Sec3 and a t-SNARE component. Our data indicate that TORC1 negatively regulates cell separation in budding yeast by participating in Cbk1 phosphorylation, which in turn controls the fusion of secretory vesicles transporting hydrolase at the site of division., Peer reviewed

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