Resultados de investigación

NNMT and HMT expression and relationship in cancer vs. normal samples., Peer reviewed

Relationship of other groups of methyltransferases to NNMT in cancers., Peer reviewed

2 tables, 2 figures, Supporting information for the article https://doi.org/10.3390/su14116472, Table S1 – Relative abundance of molecular species of fatty acids (expressed as % of total pool of fatty acids) of Hermetia illucens larvae fed with different substrates retrieved from the literature surveyed.-- Table S2: Dataset used on MetaboAnalist to perform the statistical analysis.-- Table S3 - Top 10 peer-reviewed scientific journals publishing scientific research addressing the fatty acid profile of Hermetia illucens retrieved from WoS™ and Scopus. (Journals publishing 5 or less articles on this topic were grouped as Others).-- Figure S1: Box plots and kernel density plots before and after normalization. The boxplots show at most 50 features due to space limitations. The density plots are based on all samples. Data transformation: Log Normalization; Data scaling: Autoscaling.-- Figure S2: Hierarchical clustering of substrates shown as dendrogram (distance measures using Euclidean, and clustering algorithm using Ward’s Distance).-- References, Peer reviewed

In melanoma NNMT expression is strongly anticorrelated with the histone methyltransferase-encoding gene SETDB1, a known driver of melanoma., Peer reviewed

Infiltration of specific immune cell types into primary tumours correlates positively with NNMT expression but does not strongly confound the HMT-NNMT relationship., Peer reviewed

Total histone methyltransferase expression is strongly anticorrelated with the activity of NNMT in cancers (related to Fig 1)., Peer reviewed

1MNA is an outlier when clustering metabolite levels across CCLE cell lines., Peer reviewed

1 zip file, containing: -Morphological data.xlsx: Mean values of each species for the four quantitative traits (LA, LW, ILB and LMA) and the five qualitative traits (Leaf Habit, Pubescence, Shape, Apex, Margin) LA: Leaf Area, expressed in cm2. LW: Lenght-Width ratio, dimensionless. ILB: Index of Lobulation, expressed as (Perimeter/square root of Area), dimensionless. LMA: dry Leaf Mass per Area, expresed as (dry weight/leaf area), expressed in g/m2. Leaf Habit: Deciduous or Evergreen Pubescence: Glabrous, Pubescent or Densely Pubescent Shape: Elliptic, Obovate, Oblong, Lanceolate, Ovate or Circular. Apex: Acute, Acuminate, Rounded or Straight Margin: Entire, Lobated, Serrate, Dentate, Undulate, Spinose or Crenate. -Climatic data.csv: Average Worldclim variables for each species used in the climatic PCA. See https://www.worldclim.org/data/bioclim.html for further details. "bio20" was calculated ex profeso for the paper as a result of BIO16 minus BIO17. -Supplementary Tables.xlsx: Supplementary material containing the five supplementary tables for the main manuscript., Files in this folder include raw data as well as supplementary files corresponding to the paper: "Oak leaf morphology may be more strongly shaped by climate than by phylogeny" Rubén Martín-Sánchez, Domingo Sancho-Knapik, David Alonso-Forn, Ana López-Ballesteros, Juan Pedro Ferrio, Andrew L. Hipp, José Javier Peguero-Pina, Eustaquio Gil-Pelegrín. Annals of Forest Science. 2024. https://doi.org/10.1186/s13595-024-01232-z In addition, you can also find the five supplementary tables generated for the same paper. Corresponding author: Rubén Martín Sánchez (rmartin@cita-aragon.es), Peer reviewed

Supplementary material table and figures, Table S1. Bacteria, oomycete, fungi, phages, plasmids and oligonucleotides used in this study. Figure S1. Halos of antibiosis of filter-sterilized supernatants of Pantoea agglomerans 9Rz4 grown in different culture media. Growth inhibition of the ascomycete yeast Schizosaccharomyces pombe (herbicolin A sensitive) with culture supernatants of P. agglomerans 9Rz4 and the herbicolin A deficient mutant NB1 grown overnight in LB, minimal medium (MM), YE and Strobel medium (Strobel et al., 1999) at 25 ºC. For the bioassays, a S. pombe top agar lawn was prepared in YE-agar and 300 µl of filter-sterilized supernatants were added to holes punched in the S. pombe bioassay plates. The size of the inhibition halos is indicative of the susceptibility of S. pombe to herbicolin A. The bioassays were repeated three times and representative pictures are shown. Picture were taken after 48 h of incubation at 30 ºC. The radius of the halos from three biological replicas is 7.0 ± 0.2 mm (LB), 4.1 ± 0.1 (MM), 6 ± 0.1 mm (YE) and 1.8 ± 0.05 mm (Strobel medium). Bars, 5 mm. Figure S2. Pantoea agglomerans 9Rz4 maize root colonization and its effect on plant growth. A, Maize plants 10 days after inoculation with 9RZ4. Non-inoculated plants were included as control. B, Root weight of maize plants shown in Fig. S2A. Shown are mean and standard deviation of six different plants. No statistically significant differences in root weight were observed between inoculated and non-inoculated plants. C, Maize root colonization assays of P. agglomerans 9Rz4 and its mutant strain NB1. Shown are mean and standard deviation of six different plants. In A-C, sterilization, germination and inoculation of maize seeds was carried out as described previously (Matilla et al., 2007), with minor modifications. Briefly, sterile maize seeds were incubated for 45 min at 30 ºC with a 107 CFU/mL of Pantoea agglomerans 9Rz4 strains. Thereafter, seeds were rinsed with sterile deionized water and planted in 50 mL tubes containing 40 g of sterile washed silica sand and 10% (v/w) plant nutrient solution supplemented with Fe-EDTA and micronutrients, as described previously (Matilla et al., 2007). Plants were maintained at 24 ºC with a daily light period of 16 h for 10 days. Figure S3. Effect of plasmid carriage on the antifungal properties of P. agglomerans 9Rz4. Bioactivity against Verticillium dahliae of 9Rz4 and a 9Rz4 variant (9Rz4-W) lacking plasmid p9Rz4_1. Pictures were taken after 96 h of incubation at 25 °C. Figure S4. Herbicolins A and B production is reduced in the quorum sensing mutant defective in the acyl-homoserine lactone synthase PagI. Abundance of herbicolins A and B relative to the wild type 9Rz4 in the supernatants of the P. agglomerans 9Rz4 strains. Data are the mean and standard deviations from three biological replicates and correspond to the intensity (area under the peak) of “extracted ion chromatograms” (EIC) shown in Fig. 6B. Note that the areas derived from the EICs are not comparable between compounds (e.g. herbicolin A vs herbicolin B) as these areas depend on the ionization efficiency of each compound. As shown in Fig. 1A, herbicolin B is found at trace levels in the 9Rz4 supernatants based on LC-HRMS analyses. Figure S5: Complementation of herbicolins A and B production in the quorum sensing mutant defective in the acyl-homoserine lactone synthase PagI. A, Growth inhibition of Schizosaccharomyces pombe with culture supernatants of Pantoea agglomerans strains. For the assays, P. agglomerans strains were inoculated at an initial OD660 of 0.05 in 10 mL of LB medium containing 1 mL supernatants of overnight cultures of P. agglomerans NB1 (herbicolin A defective; wild type in acyl-homoserine lactone production) or P. agglomerans PagI (defective in the synthesis of acyl-homoserine lactones) grown in LB medium. Then, bacterial cultures were grown overnight at 25 ºC, at which time the supernatants were collected, filter-sterilized and characterized chemically and biologically. For the bioassays, a Schizosaccharomyces pombe top agar lawn was prepared and 300 µL of filter-sterilized supernatants were added to holes punched in the S. pombe bioassay plates. Pictures were taken after 48 h of incubation at 30 ºC. Numerical values indicate the mean and standard deviation of the radius of the inhibition halo of three biological replicates. Bars, 5 mm. B, Extracted ion chromatograms (EIC) corresponding to an m/z of 659.385 ± 0.005 (theoretical value for [M+2H]2+ in herbicolin A) and to an m/z of 569.845 ± 0.005 (theoretical value for [M+2H]2+ in herbicolin B). C, Abundance of herbicolins A and B relative to the wild type strain 9Rz4 in the supernatants of the P. agglomerans 9Rz4 strains through the measurement of peak areas in the EICs shown in Fig. S5B. In B and C, note that the areas derived from the EICs are not comparable between compounds (e.g. herbicolin A vs herbicolin B) as these areas depend on the ionization efficiency of each compound. As shown in Fig. 1A, herbicolin B is found at trace levels in the 9Rz4 supernatants based on LC-HRMS analyses., Peer reviewed

20 files, Mussels (Mytilus galloprovincialis) were exposed during 24 hours to a waterborne infection with 10E8 CFU/ml Vibrio splendidus (reference strain LGP32) in the tank water. Five biological replicates were used for each infected and control conditions, APDAPD3401230109.mgf.-- APDAPD3401230109.raw.-- APDAPD3401230110.mgf.-- APDAPD3401230110.raw.-- APDAPD3401230111.mgf.-- APDAPD3401230111.raw.-- APDAPD3401230112.mgf.-- APDAPD3401230112.raw.-- APDAPD3401230113.mgf.-- APDAPD3401230113.raw.-- APDAPD3401230114.mgf.-- APDAPD3401230114.raw.-- APDAPD3401230115.mgf.-- APDAPD3401230115.raw.-- APDAPD3401230116.mgf.-- APDAPD3401230116.raw.-- README.txt.-- checksum.txt.-- peptides.pep.xml, Peer reviewed