BUSCADOR RECOLECTA

5 pages. -- Table S1. Summary table with description of sites and fish species studied at four locations across the Mediterranean Sea. -- Fig. S1 Trend in minimum (1st percentile of daily temperatures) and maximum (99th percentile of daily temperatures) sea surface temperatures from 1981 to 2019. -- Fig. S2 Ivlev’s electivity index for herbivorous fishes across the Mediterranean Sea. Ivlev’s index standardizes food consumed by food availability within the habitat and scales from -1 (extreme selection against food source) to 1 (extreme selection for food source) (Jones & Norman 1986), Peer reviewed

Climate-driven species redistributions are reshuffling the composition of marine ecosystems. How these changes alter ecosystem functions, however, remains poorly understood. Here we examine how the impacts of herbivory change across a gradient of tropicalization in the Mediterranean Sea, which includes a steep climatic gradient and marked changes in plant nutritional quality and fish herbivore composition. We quantified individual feeding rates and behaviour of 755 fishes of the native Sarpa salpa, and non-native Siganus rivulatus and Siganus luridus. We measured herbivore and benthic assemblage composition across 20 sites along the gradient, spanning 30º of longitude and 8º of latitude. We coupled patterns in behaviour and composition with temperature measurements and nutrient concentrations to assess changes in herbivory under tropicalization. We found a transition in ecological impacts by fish herbivory across the Mediterranean from a predominance of seagrass herbivory in the west to a dominance of macroalgal herbivory in the east. Underlying this shift were changes in both individual feeding behaviour (i.e., food choice) and fish assemblage composition. The shift in feeding selectivity was consistent among temperate and warm-affiliated herbivores. Our findings suggest herbivory can contribute to the increased vulnerability of seaweed communities and reduced vulnerability of seagrass meadows in tropicalized ecosystems., Funding: Fundación BBVA, Award: Interbioclima; Ministry of Economy, Industry and Competitiveness, Award: CGL2015-71809-P, Fig._1_reconsructed_temperatures_by_location.csv, Fig._2_benthic_data_RAW.csv, Fig._2_herbivore_abundance_biomass.csv, Fig._3_Herbivore_bite_rates.csv, Fig._4b_C_and_N_Posidonia_Padina_turf.csv, Peer reviewed

6 pages. -- Figure S1. Schematic circulation. -- Figure S2. Wind stress variability. -- Figure S3. Seasonal shelf circulation anomaly. -- Figure S4. AAO & SOI. -- Figure S5. Antarctic Dipole., Peer reviewed

14 pages. -- A1. Synthetic approach. -- A1.1. General description. -- A1.2. Numerical approach. -- A1.3. GWHP scenarios. -- A1.4. Results. -- A1.4.1. Concentration and groundwater temperature across the aquifer. -- A1.4.2. Evolution of concentration and groundwater temperature on the downgradient boundary. -- Appendix B. -- B1. Sensitivity analysis. -- B1.1. Hydraulic conductivity. -- Appendix C. Concentration of OCECs reported at the study site is shown in the two plots of Figure C1., Peer reviewed

[Description of methods used for collection/generation of data] HEI-193 vestibular schwannoma cell lines, healthy human Great Auricular Nerve and vestibular schwannoma tumor samples, drug treatments, cell viability assay, In vitro senescence associated B-galactosidase activity assay, cell cycle analysis, western blotting, RNA extraction and expression analysis, annexin V-FITC and propidium iodide dual staining, immunofluorescence assays., [Background] Vestibular schwannomas (VS) are complex and heterogeneous human tumors arising from the Schwann cell compartment of the vestibulocochlear nerve. VS cause significant neurological deficit such as hearing loss and vestibular impairment, and in some cases death due to brainstem compression. There is an urgent need to find pharmacotherapies for VS since surgical removal and stereotactic radiosurgery are the only effective treatments. Cancer therapy based in the combination of drug-induced senescence and senolytics may provide an innovative pharmacological alternative for VS management., [Methods] Senescence-associated β-galactosidase (SA-β-GAL) activity detection assay, real-time polymerase chain reaction (RT-PCR), western blotting and immunofluorescence, together with viability assays were used to analyze the response to different chemotherapy drugs of the human VS HEI-193 cell line. Human VS tumor paraffin sections were also studied for SA-β-GAL-stained cells., [Results] We found that chemotherapy compounds induced genotoxic stress and cellular senescence in HEI-193 VS cells, as characterized by increased SA-β-GAL activity, growth arrest, increased levels of the cyclin-dependent kinase inhibitor p21 and the accumulation of DNA damage. These cellular senescence markers were also accompanied by an increase of senescence-associated secretory phenotype (SASP): IL6, IL8, IL1B and MMP1. Induction of senescence by chemotherapy rendered HEI-193 VS cells as druggable targets for senolytic compounds, as navitoclax. Thus, treatment with navitoclax selectively eliminated bleomycin-induced senescent HEI193 VS cells by activating the extrinsic and intrinsic apoptosis pathways. Our data also show the presence of senescent cells, SA-β-GAL-positive stain, in human VS tumors, which are not present in healthy great auricular nerve sections., [Conclusions] These findings suggest that a one-two punch strategy of pro-senescence therapy induced by chemotherapy treatment followed by senolytic therapy represents a new paradigm for the pharmacological treatment of VS., This research was funded by Spanish MCIN/ AEI/ 10.13039/ 501100011033THEARPY-PID2020-115274RB-I00,MINA-CM Madrid Innovative Neurotech Alliance P2022/BMD-7236, CA20113 COST Action EU-ProteoCure, CA23119 COST Action EU-SENESCENCE2030: Targeting Cell Senescence to Prevent Age-Related Diseases and the Spanish Senescence Network, Senestherapy (RED2018-102698-T) FEDER/MICIN grants to Isabel Varela Nieto., Figure_1_ Figure_2, Figure_3, Figure_4, Figure_5, Figure_6., Peer reviewed

[Description of methods used for collection/generation of data] We deployed 12 trail cameras in Montaña Palentina (Acorn LTL5310 (Shenzhen, China) =9; Bushnell Trophy Cam HD Black Led (Kansas, USA) = 3) and 12 in Alto Sil (Acorn LTL5310 (Shenzhen, China) =9; Bushnell Trophy Cam HD Black Led (Kansas, USA) = 2, and Browning Dark OPS (Herstal, Belgium) = 1), between July and September 2023 (ca. 60 days per camera) aiming at detecting European wildcats. Inside each location, cameras were distributed within an area of roughly 2000 Has at distances of ca. 1500 m from each other, as this procedure is reliable to estimate European wildcat densities (Gil-Sánchez et al., 2015). Cameras were placed 30-50 cm above ground pointing perpendicularly at paths naturally used by wild animals and from which they were 2-3 m away. An open Eppendorf tube filled with Iberian lynx (Lynx pardinus) urine was buried in front of each camera, aiming at causing European wildcats to stop in front of the camera to optimize identification of individuals based on pelage patterns. Cameras were checked ca. every 20 days to download pictures and replace the batteries and urine (i.e. 2 reviews after deployment and finally camera retrieval). All cameras were programmed to take a burst of three pictures per trigger. Sensitivity was set to low to minimize the excess of triggers caused by sun glares or moving branches. Cameras used natural and infrared light during day and night triggers respectively., [Methods for processing the data] Pictures were sorted following the methodology of R package CamtrapR (Niedballa et al. 2016) in order to extract metadata (date and time of the picture). Humans and birds were not included., [EN] This dataset provides the raw results of a mammalian survey campaign using trail cameras conducted between July and September 2023 (1495 trap-nights) simultaneously in Montaña Palentina Natural Park (Palencia, Spain) and Alto Sil (León, Spain). The main objective of the survey was to detect and estimate population numbers for European wildcats (Felis silvestris), but several small and large mammal species (Carnivora, Artiodactyla, Rodentia and Lagomorpha) were detected., [ES] Este conjunto de datos proporciona los resultados brutos de la campaña de fototrampeo desarrollada entre Julio y Septiembre de 2023 (1495 noches-trampa) de forma simultánea en el Parque Natural de Montaña Palentina (MNPN, Palencia, España) y el Alto Sil (León, Spain). El principal objetivo del muestreo era detectar y estimar números poblacionales en el gato montés europeo (Felis silvestris), pero otras especies de mamíferos (Carnivora, Artiodactyla, Rodentia and Lagomorpha) también fueron detectadas., This study was funded by a contract of collaboration between Fundación Reina Sofia and CSIC, the MICINN through the European Regional Development Fund [SUMHAL, LIFEWATCH-2019-09-CSIC-04, POPE 2014-2020], and Land Rover España., Peer reviewed

5 pages. -- Generating the simulated datasets. -- Testing the function. -- Additional tests. -- Fig. S1. Simulated datasets for 10 individuals., Peer reviewed

24 pages. -- Table S1: R functions developed specifically for this paper to test the effects of individual site fidelity, temporal and spatial variability in tracking data. The use of the functions and their arguments are described in detail. -- Table S2: summary of logger and deployment details for each colony, prior publication of the data used for this study, and permits and licences granted for the corresponding fieldwork. -- Table S3: number of individuals tracked repeatedly for each colony. -- Table S4: number of tracks per year and colony in the dataset used to run the year effect test (no repeated individuals). -- Table S5: population estimates from all breeding colonies of Cory’s, Scopoli’s and Cape Verde shearwaters available in the publisher literature. n refers to the number of tracks obtained from each colony. -- Figure S1: significant relationship between species inclusiveness values and longitude (top) and population size (breeding pairs) in the log scale (bottom) for Scopoli’s shearwater. -- Figure S2: schematic image of the main currents exploited by Cory’s and Scopoli’s shearwaters around the African continent., Peer reviewed

A tabular spreadsheet with the species, colony, unique identifiers for each bird and trip, latitude, longitude, year of tracking (1st year, 2nd year, etc.), and an ordering column that allows the positions to be ordered to form a track., on-breeding locations of Cory’s shearwaters (Calonectris borealis), Scopoli’s shearwaters (C. diomedea), and Cabo Verde shearwaters (C. edwardsii) tracked from the colonies of Berlenga, Chafarinas, Corvo, Faial, Graciosa, Montaña Clara, Pico, Selvagem, Sisargas, Terreros, Timanfaya, Veneguera, and Vila for Cory’s shearwaters; Cala Morell, Chafarinas, Filfla, Frioul, Giraglia, Gozo, Lavezzi, Linosa, Malta, Na Foradada, Na Pobra, Palomas, Pantaleu, Paximada, Porquerolles, Riou, Strofades, Tremiti, and Zembra for Scopoli’s shearwaters; and Curral Velho and Raso for Cabo Verde’s shearwaters. Animals were tracked between the years of 2006 and 2016, and data includes species, colony, unique identifiers for each bird and trip, latitude, longitude, year of tracking (1st year, 2nd year, etc.), and an ordering column that allows the positions to be ordered to form a track., Peer reviewed

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