Resultados de investigación

CCLE NNMT-HMT correlation all cancers full labelled plots., Peer reviewed

7 files, Knowledge of the three-dimensional movement patterns of elasmobranchs is vital to understanding their ecological roles and exposure to anthropogenic pressures. To date, comparative studies among species at global scales have mostly focused on horizontal movements. Our study addresses the knowledge gap of vertical movements by compiling the first global synthesis of vertical habitat use by elasmobranchs from data obtained by deployment of 989 biotelemetry tags on 38 elasmobranch species. Elasmobranchs displayed high intra- and interspecific variability in vertical movement patterns. Significant vertical overlap was observed for many epipelagic elasmobranchs, indicating an increased likelihood to display spatial overlap, biologically interact, and share similar risk to anthropogenic threats that vary on a vertical gradient. We highlight the critical next steps towards incorporating vertical movement into global management and monitoring strategies for elasmobranchs, emphasising the need to address geographic and taxonomic biases in deployments and to concurrently consider both horizontal and vertical movements, Peer reviewed

Subcellular localisations of transsulphuration and glutathione synthesis genes., Peer reviewed

Differential TF activity in Ahcy loss of function experiments., Peer reviewed

HMT correlations to transcription factor activity in GTEx., Peer reviewed

The N-terminal tails of eukaryotic histones are frequently posttranslationally modified. The role of these modifications in transcriptional regulation is well-documented. However, the extent to which the enzymatic processes of histone posttranslational modification might affect metabolic regulation is less clear. Here, we investigated how histone methylation might affect metabolism using metabolomics, proteomics, and RNA-seq data from cancer cell lines, primary tumour samples and healthy tissue samples. In cancer, the expression of histone methyltransferases (HMTs) was inversely correlated to the activity of NNMT, an enzyme previously characterised as a methyl sink that disposes of excess methyl groups carried by the universal methyl donor S-adenosyl methionine (SAM or AdoMet). In healthy tissues, histone methylation was inversely correlated to the levels of an alternative methyl sink, PEMT. These associations affected the levels of multiple histone marks on chromatin genome-wide but had no detectable impact on transcriptional regulation. We show that HMTs with a variety of different associations to transcription are co-regulated by the Retinoblastoma (Rb) tumour suppressor in human cells. Rb-mutant cancers show increased total HMT activity and down-regulation of NNMT. Together, our results suggest that the total activity of HMTs affects SAM metabolism, independent of transcriptional regulation., Peer reviewed

HMT correlations to transcription factor activity in TCGA., Peer reviewed

1 file, To evaluate changes in terrestrial and aquatic ecosystems in the Azores, we developed paleoecological and paleoclimate records from Lake Funda on Flores Island that span the last millennium. Vegetation composition (n-alkane average chain length) indicate when human activities began in the catchment area and biogenic silica tracks changes in diatom productivity. In addition, sterol hydrogenation and archaeal lipids (isoprenoid glycerol dialkyl glycerol tetraethers) trace changes in redox conditions and biogeochemical cycles, respectively. Finally, a high-resolution reconstruction using leaf wax hydrogen isotopes records changes in precipitation amount over the last millennium, Fundação para a Ciência e Tecnologia (FCT), grant/award no. PTDC/CTA-AMB/28511/2017: WHEN WERE THE AZORES ARCHIPELAGO REALLY COLONIZED? A HIGH-RESOLUTION PALEOLIMNOLOGICAL APPROACH, Peer reviewed

The N-terminal tails of eukaryotic histones are frequently posttranslationally modified. The role of these modifications in transcriptional regulation is well-documented. However, the extent to which the enzymatic processes of histone posttranslational modification might affect metabolic regulation is less clear. Here, we investigated how histone methylation might affect metabolism using metabolomics, proteomics, and RNA-seq data from cancer cell lines, primary tumour samples and healthy tissue samples. In cancer, the expression of histone methyltransferases (HMTs) was inversely correlated to the activity of NNMT, an enzyme previously characterised as a methyl sink that disposes of excess methyl groups carried by the universal methyl donor S-adenosyl methionine (SAM or AdoMet). In healthy tissues, histone methylation was inversely correlated to the levels of an alternative methyl sink, PEMT. These associations affected the levels of multiple histone marks on chromatin genome-wide but had no detectable impact on transcriptional regulation. We show that HMTs with a variety of different associations to transcription are co-regulated by the Retinoblastoma (Rb) tumour suppressor in human cells. Rb-mutant cancers show increased total HMT activity and down-regulation of NNMT. Together, our results suggest that the total activity of HMTs affects SAM metabolism, independent of transcriptional regulation., Peer reviewed

5 files.-- The CETUS dataset contains effort-based occurrence records collected during a cetacean monitoring programme in the Eastern North Atlantic, since 2012, The CETUS dataset contains data collected within the CETUS Project (www.cetusproject.com), a cetacean monitoring programme in the Eastern North Atlantic, running since 2012. The project is led by the Interdisciplinary Centre of Marine and Environmental Research (CIIMAR - University of Porto, Portugal), in partnership with TRANSINSULAR - Grupo ETE, a Portuguese company for maritime transport, that offers its cargo ships to be used as a platforms of opportunity to monitor cetaceans along routes between Continental Portugal and Madeira, Azores, Canary and Cape Verde islands. On-board trained marine mammal observers collect data on: survey effort, cetacean and other megafauna occurrences, as well as marine traffic and meteorological conditions. Data is provided in the recent OBIS-ENV-DATA format, and comprises 8913 georeferenced positions associated with 3195 occurrences of 44 marine taxa, This research was partially funded by the EU FEDER/FEMP and Portuguese Foundation for Science and Technology (FCT) under the Portugal2020 (Lisboa2020, Algarve2020 and MAR2020) Programme through project OBSERVA.PT (MAR-01.04.02-FEAMP-0002), Peer reviewed