BUSCADOR RECOLECTA

Soft tissue defects or pathologies frequently necessitate the use of biomaterials that provide the volume required for subsequent vascularization and tissue formation as autrografts are not always a feasible alternative. Supramolecular hydrogels represent promising candidates because of their 3D structure, which resembles the native extracellular matrix, and their capacity to entrap and sustain living cells. Guanosine-based hydrogels have emerged as prime candidates in recent years since the nucleoside self-assembles into well-ordered structures like G-quadruplexes by coordinating K+ ions and π–π stacking, ultimately forming an extensive nanofibrillar network. However, such compositions were frequently inappropriate for 3D printing due to material spreading and low shape stability over time. Thus, the present work aimed to develop a binary cell-laden hydrogel capable of ensuring cell survival while providing enough stability to ensure scaffold biointegration during soft tissue reconstruction. For that purpose, a binary hydrogel made of guanosine and guanosine 5'-monophosphate was optimized, rat mesenchymal stem cells were entrapped, and the composition was bioprinted. To further increase stability, the printed structure was coated with hyperbranched polyethylenimine. Scanning electron microscopic studies demonstrated an extensive nanofibrillar network, indicating excellent G-quadruplex formation, and rheological analysis confirmed good printing and thixotropic qualities. Additionally, diffusion tests using fluorescein isothiocyanate labeled-dextran (70, 500, and 2000 kDa) showed that nutrients of various molecular weights may diffuse through the hydrogel scaffold. Finally, cells were evenly distributed throughout the printed scaffold, cell survival was 85% after 21 days, and lipid droplet formation was observed after 7 days under adipogenic conditions, indicating successful differentiation and proper cell functioning. To conclude, such hydrogels may enable the 3D bioprinting of customized scaffolds perfectly matching the respective soft tissue defect, thereby potentially improving the outcome of the tissue reconstruction intervention., Peer reviewed

17 Pág., Forest biomass is an essential resource in relation to the green transition and its assessment is key for the sustainable management of forest resources. Here, we present a forest biomass dataset for Europe based on the best available inventory and satellite data, with a higher level of harmonisation and spatial resolution than other existing data. This database provides statistics and maps of the forest area, biomass stock and their share available for wood supply in the year 2020, and statistics on gross and net volume increment in 2010-2020, for 38 European countries. The statistics of most countries are available at a sub-national scale and are derived from National Forest Inventory data, harmonised using common reference definitions and estimation methodology, and updated to a common year using a modelling approach. For those counties without harmonised statistics, data were derived from the State of Europe's Forest 2020 Report at the national scale. The maps are coherent with the statistics and depict the spatial distribution of the forest variables at 100 m resolution., We thank the ENFIN and the NFI organisations and national correspondents of 26 European countries who participated in the data harmonisation study supported by the JRC Specific Contracts n. 13, 17, 18, 19, 20 and 21 for the production of data harmonised for definition and estimation method regarding forest biomass, forest area and biomass not available for wood supply, and forest increment. The study was partly funded by the Framework contract for the provision of forest data and services in support to the European Forest Data Centre, Contract numbers 388432 (2012–2017) & 934340 (2017–2021). The views expressed are purely those of the writers and may in no circumstance be regarded as stating an official position of the European Commission., Peer reviewed

N-heterocyclic carbene (NHC) iridium (III) complexes are promising for the use as blue emitters in organic light-emitting diodes. Exciton transfer between such organometallic complexes is investigated using time-dependent density functional theory calculations. Casida's equation is solved to study absorption and emission of the neutral and charged complexes using the ORCA package. The Sternheimer equation implemented in the Octopus code is extended to take into account spin-orbit coupling and is applied to investigate triplet excitations. Real-time propagation as implemented in the Octopus code is used to simulate exciton dynamics in an emitter dimer and to extract the exciton coupling via explicit integration of transition densities., The version of the Octopus code used for the solution of the Sterhneimer equation with account of spin-orbit coupling is enclosed. The authors thank the financial support from the EU-H2020 project ``MOSTOPHOS" (n. 646259). JJS gratefully acknowledges the funding from the European Union Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 795246-StrongLights., Peer reviewed

Carbon storage is widely acknowledged as one of the most valuable forest ecosystem services. Deforestation, logging, fragmentation and climate change have significant impacts on tropical carbon stocks, however an elusive and yet undetected decrease in carbon storage may be due to defaunation of large seed dispersers. Many large tropical trees with sizeable contributions to carbon stock rely on large vertebrates for seed dispersal and regeneration, yet many of these frugivores are threatened by hunting, illegal trade and habitat loss. We used a large dataset on tree species composition and abundance, seed, fruit and carbon related traits, and plant-animal interactions to estimate the loss of carbon storage capacity of tropical forests in defaunated scenarios. By simulating the local extinction of trees that depend on large frugivores at 31 Atlantic Forest communities, we found that defaunation has the potential to significantly erode carbon storage even when only a small proportion (10%) of large-seeded trees are extirpated., Peer reviewed

Abundance table, auxiliary data, phylogeny and code for the particle-attached and free-living prokaryotes detected in 30 globally distributed stations from the Malaspina 2010 Expedition. Corresponds to 16SrDNA amplicons Illumina-based sequencing., Peer reviewed

Diffraction images of a native crystals of a fragment of human plectin that includes the first and second spectrin repeats (SR1-SR2) of the plakin domain. The two Cys in the wild type sequence were replaced by Ala. Images correspond to the dataset used to refine the pdb entry 2ODV (http://www.rcsb.org/pdb/explore/explore.do?structureId=2ODV). Data was collected at the BM14 beamline of the European Synchrotron Radiation Facility (ESRF, Grenoble, France) using radiation of 0.9785 Å wavelength and a Mar CCD detector. The dataset consists of 360 images (1 degree oscillation per image). Data extend to ~1.85 Å resolution., Peer reviewed

Diffraction images of native crystals of the Calx-β domain of the human integrin β4 subunit. Images correspond to the dataset used to solve and refine the pdb entry 3FQ4 (http://www.rcsb.org/pdb/explore/explore.do?structureId=3FQ4). Data was collected using a rotating anode generator (MicrostarH, Bruker AXS) and a mar345dtb image plate detector (marXperts GmbH). The dataset consists of 360 images (1 degree oscillation per image). Data extend to 1.48 Å resolution., Peer reviewed

Supplementary material for the journal article "De novo MYC addiction as an adaptive response of cancer cells to CDK4/6 inhibition". 13C Metabolic flux analyses of CDK4/6 knockdown HCT116 cells and control HCT116 cells performed using INCA v1.5 (Young, J. D., 2014, INCA: a computational platform for isotopically non-stationary metabolic flux analysis. Bioinformatics 30, 1333-1335; http://mfa.vueinnovations.com.) running with MATLAB R2012b. The two files were generated by INCA with the estimated flux map distributions, network model, and tracer simulations., Peer reviewed

Diffraction images of crystals of a fragment of the plakin domain of human plectin that includes the spectrin repeats 7 to 9 (SR7-SR9). Images correspond to the dataset used to solve and refine the pdb entry 5J1I (http://www.rcsb.org/pdb/explore/explore.do?structureId=5J1I). Data were collected on a single crystal at the beamline 14.2 of the European Synchrotron Radiation Facility (ESRF, Grenoble, France) using radiation of 0.9330 Å wavelength and an ADSC Q4 CCD detector. The dataset consists of 360 images (1 degree oscillation per image). Diffraction data is highly anisotropic. Based on analysis with the STARANISO server (http://staraniso.globalphasing.org/) data extend approximately to 5.0, 3.8, and 2.6 Å resolution along the three principal directions of anisotropy, which are 0.555 a*+ 0.832 c*, b*, and -0.361 a* + 0.932 c*, respectively., This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund, Grants BFU2012-32847 and BFU2015-69499-P. We thank the ESRF for providing access to synchrotron radiation facility., Peer reviewed

1. Computational details 2. Phonon dispersion curves of the fourteen skutterudite-like MP3 compounds 3. Band structures of the skutterudite-like YP3 and LaP3 4. The PDOS of the stable skutterudite-like MP3 compounds 5. The crystal structures of skutterudite-like CeP3 and hypothetical skutterudite-like Ce0P3 model 6. The electronic band structures of the hypothetical skutterudite-like Ce0P3 and Ce0S3 at 0 GPa 7. The Fermi surfaces of skutterudite-like CeP3 at 0 GPa 8. The projected electronic band structures of the three bands crossing the Fermi Level of skutterudite-like CeP3 at 0 GPa 9. The convex hull of Ce-P compounds at 25 GPa 10. The transition barrier required to revert the skutterudite-like CeP3 to the conventional skutterudite-type CeP3 at 0 GPa 11. The relative enthalpies of the proposed skutterudite-like CeP3 with respect to the binary Ce-P compounds and elemental solids Ce and P at 0 GPa 12. Structural information of the proposed skutterudite-like CeP3 phase 13. Superconducting parameters of the proposed skutterudite-like CeP3 phase at 0 and 25 GPa 14. References, Peer reviewed