BUSCADOR RECOLECTA

Table S1: Structures and activity of disulfiram and dithiocarbamate derivatives., Peer reviewed

9 figures, 3 tables, 1 video.-- 2. X-ray diffraction and scanning electron microscopy: The change in the wettability properties of the pristine felt is evidenced in the following video sequence, where some drops of water were poured on top of the felt (both pristine and rGO-PEG modified felt). When the drop is deposited on the surface of the pristine felt (Figure S5, left) it remains intact on its surface indicating some degree of hydrophobicity. Whereas, when water drops are deposited on the rGO-modified felt (Figure S5, right), they permeate into the interior of the felt. This shows that the impregnation of the felt with rGO-PEG increases its hydrophilicity, favoring the water/felt interphase contact and the diffusion of the aqueous electrolyte inside the felt. Hydrophilicity can also help to prevent the retention of air bubbles during the assembly of the flow cell and improve electrolyte flow distribution through the electrode.-- 5. Randles-Sevčik Analysis: To estimate the comparative surface area of the pristine and modified felts, a Randles-Sevčik was performed on the voltammograms with the knowledge of the reported diffusion coefficient.-- Under a Creative Commons Licenses CC-BY 4.0., 1. XPS analysis.-- 2. X-ray diffraction and scanning electron microscopy.-- 3. Peak Current Analysis.-- 4. Cyclic Voltammetry in Acid Solution.-- 5. Randles-Sevčik Analysis.-- 6. Cell Voltage vs. Time Plots, Peer reviewed

We analyzed 479 sampled communities from 299 sites around the planet from the Reef Life Survey (RLS) database (Edgar et al, 2020) comprising population data from more than 1,500 non-benthic marine species with individual body size information. Body size was measured as biomass and data were aggregated by year. We included only sampling sites in our analysis, which were surveyed more than once per year. This decision is based on a prior rarefaction analysis that we conducted to assess the impacts of sampling effort from the RLS database due to the noisiness of one sampling event in species richness. We collected weekly sea surface temperature (SST) from NOAA's (National Oceanic and Atmospheric Administration) remote sensing database. In our analysis, we used the sum of thermal stress anomalies (TSA), calculated as the number of events when the average difference between weekly SST and the maximum weekly climatological SST was above 1 degree Celsius between 1982 and 2019. The distribution of warm-water coral reef was obtained from UNEP-WCMC World Fish Centre database. The average trophic level of species was obtained from Fishbase database. Finally, the information on marine protected areas was obtained from UNEP-WCMC and IUCN Protected Planet database. The information on human population density was obtained from Gridded Population of the World. The human population density was quantified as humans/Km2 in a 25-km radius around the sampling site. Lastly, we used the regression coefficient between log biomass and log of average body sizes as a measure of community structure. The "data.csv" file contains all raw data used to calculate the final results in the "results.csv" file. The variables and their definitions in "results.csv" are the following:, The sustainability of marine communities is critical for supporting many biophysical processes that provide ecosystem services that promote human well-being. It is expected that anthropogenic disturbances such as climate change and human activities will tend to create less energetically-efficient ecosystems that support less biomass per unit energy flow. It is debated, however, whether this expected development should translate into bottom-heavy (with small basal species being the most abundant) or top-heavy communities (where more biomass is supported at higher trophic levels with species having larger body sizes). Here, we combine ecological theory and empirical data to demonstrate that full marine protection promotes shifts towards top-heavy energetically-efficient structures in marine communities. First, we use metabolic scaling theory to show that protected communities are expected to display stronger top-heavy structures than disturbed communities. Similarly, we show theoretically that communities with high energy transfer efficiency display stronger top-heavy structures than communities with low transfer efficiency. Next, we use empirical structures observed within fully protected marine areas compared to disturbed areas that vary in stress from thermal events and adjacent human activity. Using a nonparametric causal-inference analysis, we find a strong, positive, causal effect between full marine protection and stronger top-heavy structures. Our work corroborates ecological theory on community development and provides a quantitative framework to study the potential restorative effects of different candidate strategies on protected areas., Peer reviewed

Peer reviewed

This is a dataset of our research realized recently, which contains tested results (json files) by F-UJI tool and FAIR assesment reports of tested repositories., Peer reviewed

Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir–Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers., Peer reviewed

The dataset contains four volumes: two ExM image volumes and two 3D instance segmentation volumes., We curated the saturated nuclei segmentation annotation for two expansion microscopy (ExM) volumes by two neuroscience experts from a day 7 post-fertilization (dpf) zebrafish brain, imaged with confocal microscopy. These volumes have an anisotropic resolution of 0.325×0.325×2.5 μm in (x, y, z) order, with an approximate tissue expansion factor of 7.0. Thus the effective resolution becomes 0.046 × 0.046 × 0.357 μm. The two volumes are of size 2048×2048×255 voxels with 9.6K and 8.8K nuclei, respectively. This dataset is named the NucExM dataset, which serves as a testbed for 3D microscopy instance segmentation systems., National Science Foundation [1835231; 2124179], Peer reviewed

Three arguments support the involvement of actors in the design and implementation of the Monitoring and Evaluation (M&E) for Responsible Research and Innovation (RRI). First, it strengthens the evaluation; second, it allows taking advantage of the performative function; third, it is aligned with the concept of RRI. In Europe, the trend to institutionalize RRI triggered an interest in developing specific M&E methods and tools, but how actors participate in these processes is still being determined. This paper investigates the extent to which the participation of actors occurs or is expected to occur by using the three stages of translation proposed by Callon and colleagues – problematization, development of the research, and transfer to a real setting. Through a systematic review of 25 approaches developing M&E mechanisms, our findings show that participation occurs or is foreseen mainly in the later stages and is specially linked with learning and trust-related purposes., This work was supported by the Ministry of Economy Industry and Competitiveness (Spain) under Grants CSO2016-76828-R and BES-2017-081141., Peer reviewed

Supplementary Note 1: Bioinformatic analysis of Tse5 homologues (3).-- Supplementary Tables: Supplementary Table 1. Cryo-EM data collection processing and model refinement statistics (5).-- Supplementary Table 2. Descriptive graph describing the type of protein-protein interactions between Tse5-NT and Tse5-Shell calculated using Mapiya online server [5] (6).-- Supplementary Table 3. Summary of Small-angle X-ray scattering data analysis for Tse5 and Tse5 variants (7).-- Supplementary Table 4. Strains and Plasmids used in this study (8).-- Supplementary Table 5. Residues lining the interior of the Tse5-Shell that diverge from the consensus sequence found in Tse5 homologues containing C-terminal toxic fragments of putative/known enzymatic functions (10).-- Supplementary Table 6. Residues lining the exterior of the Tse5-Shell that diverge from the consensus sequence found in Tse5 homologues containing C-terminal toxic fragments of putative/known enzymatic functions (11).-- Supplementary Figures: Supplementary Figure 1. Workflow for cryo-EM structure determination (13).-- Supplementary Figure 2. Cryo-EM Structural determination of Tse5 (14).-- Supplementary Figure 3. Tse5-Shell encapsulates the pore-forming Tse5-CT toxin (16).-- Supplementary Figure 4. Residues Ser32-Lys47 anchor Tse5-NT fragment to Tse5-Rhs fragment through protein-protein interactions (17).-- Supplementary Figure 5. Tse5 is sensible to the membrane potential directionality (18).-- Supplementary Figure 6. Phylogenetic analysis of C-terminal fragments found in Tse5 homologues (19).-- Supplementary Figure 7. Alignment of Tse5 with homologues containing C-terminal toxic fragments of putative/known enzymatic functions (20).-- Supplementary Figure 8. Electrostatic surface potential of Tse5, Rhs1 and RhsA (21). Supplementary Figure 9. Protein-membrane interactions and protein stability during MD simulation (22). Supplementary Figure 10. Tse5 sequence derived for structural and biophysical studies (23).-- Supplementary Figure 11. Uncropped and unedited SDS-gels (24).-- Supplementary Note 2: LC-ESI-MS report for Tse5 (25).-- Supplementary Note 3: N-terminal sequencing report Tse5-NT (29).-- Supplementary Note 4: N-terminal sequencing report Tse5-Shell (37).-- Supplementary Note 5: N-terminal sequencing report Tse5-CT (45).-- Supplementary references (53)., Peer reviewed

Fig. S1.1 Adaptive inference protocol of the Doñana long-term social-ecological research program (based on Holling and Allen 2002, Peer reviewed