Resultados de investigación

Peer reviewed

13 pages. -- File includes: Supplementary Text, Supplementary Figures and tables. -- Figure SI1. Illustrations by dbRDA of the relationships between the predictors modelled and the concentration of microplastics from the DistLM analysis of all sampling stations (n = 28) (the best model, see table 1). -- Figure SI2 SEM image (left) and Optic Microscopy image (right) of two stations sampled. -- Figure SI3. FTIR spectra of microplastic collected from the Gulf of Cadiz. FTIR spectra contain a mixture of all these plastics . -- Figure SI4. Rainfall and Dam freshwater discharge in the Guadalquivir estuary: Average monthly rainfall and dam discharges between March and June from 1997 to 2018. -- Figure SI5. The currents at the near‐surface level (cm/s) superimposed with sea surface temperature (SST in the rage 17-20ºC) and chlorophyll concentration (Chl-a, in the range 0-3.2 mg / m3) for 15th June 2019. -- Table SI1. Dataset with station name, concentration of microplastic, temperature (ºC), salinity, chlorophyll a fluorescence (mg m-3), oxygen (mg L-1), longitude and latitude in June 2019 between the days 14 and 19. -- Table SI2. Test statistics for the Distance-based Linear Model (DISTLM) analyses of the marginal and sequential tests based on ‘Step-wise’ procedure and AICc criteria of the microplastics at the 28 sites sampled. -- Table SI3. Information collected about MP surveys in environmental marine environments., Peer reviewed

2 pages. -- Supplementary Figure 1. Growth curve of cultured Symbiodiniaceae isolated from Cotylorhiza tuberculata. -- Supplementary Figure 2. Scanning electron microscopy (SEM) coupled with EDS (Energy Dispersive X-Rays spectroscopy) for chemical characterization of the calcifying matrix found in Symbiodiniaceae cultures. -- Supplementary Figure 3. Symbiodiniaceae living inside the jellyfish Cotylorhiza tuberculata in their typical coccoid stage in hospite., The photosymbiosis with host-specific dinoflagellates is a widespread relationship in marine organisms. Despite the evidenced biodiversity of this kind of mutualism, most research focuses on the study of scleractinian corals, and there is a lack of knowledge about other symbiotic cnidarians such as jellyfishes. The Mediterranean jellyfish Cotylorhiza tuberculata (Rhizostomae, Scyphozoa) harbors an endosymbiotic dinoflagellate of the family Symbiodiniaceae. In this study, we examine the algae distribution within the host body as well as, the pigment content and cell density of the symbiont. Furthermore, the size, morphology and fluorescence of cultured symbionts were studied under light microscopy, Imaging Flow Cytometry (IFC), and Scanning Electron Microscopy (SEM). The C:N composition and optical properties of the medusa tissue were measured to evaluate their role in the symbiosis. The medusae body was divided into two different sections to investigate the distribution of symbionts in hospite: oral arms (OA) and umbrella (UM). C:N composition of C. tuberculata was and symbiont density was significantly higher in the OA section. Mean chlorophyll a concentration of the algae was 1.33 (± 0.83) pg Chl a cell–1. The study of the pigment composition by HPLC (High Performance Liquid Chromatography), revealed the presence of 13 different pigments, being the most representative chlorophyll a, chlorophyll c2, and peridinin typical pigments of Symbiodiniaceae. Cell diameter of algae freshly isolated from the host was 8.71 ± 0.97 μm and cell growth rate was 0.52 (± 0.09) 106 cell ml–1 d–1. The presence of vegetative coccoid cells, doublet and motile mastigotes were revealed within the Symbiodiniaceae cultures. A calcifying matrix typical of Symbiodiniaceae and formed in partner with bacteria, was also observed most cultures. The umbrella tissue of the medusa absorbed at ultraviolet radiation (UVR) region, suggesting that medusae tissue protects photosymbionts from the negative effect of the high energetic UVR and attenuates the light intensity reaching algae inside the host. The presence of a dense Symbiodiniaceae population and the protection to UVR and elevated environmental irradiance provided by medusae tissue, maintain symbionts in optimal light conditions for photosynthesis and may be a reason added to explain the population success of Cotylorhiza tuberculata., Peer reviewed

Peer reviewed, This PDF file includes: Supplementary notes 1-4 Supplementary discussion Supplementary Figures 1-5 Supplementary Table 1 Supplementary References

Index of supplemental materials: Table S1. Species information and accession number for sequences retrieved from public databases included in phylogenetic analysis. Table S2. Uricase (urate oxidase) partial sequences for YLS obtained from C. brasiliensis and C. orchidearum, and four Fulgoroidea species samples in Costa Rica. Table S3. Ant species associated with colonies of Cerataphis spp. and plant host combinations observed in Costa Rica and/or reported worldwide., Peer reviewed

1 vídeo., Monitoring water systems under extreme conditions or in remote areas poses a risk to scientists and staff involved, and can also result in disturbances to the local flora and fauna. In order to overcome these limitations, new techniques are being developed with the aim to gain more insight into how geochemical pathways of trace elements regulate the functioning of the ocean. Here, we present the first trace metals and inorganic nutrients concentrations measured in dissolved (<0.22 µm) surface water samples collected in the Antarctic by using an Automatic Water Autosampler (AWA) system onboard an unmanned aerial vehicle (UAV). The AWA system has been used to chemically quantify the composition of the water masses (lake and coastal water) of Deception Island (South Shetland Islands, Antarctica), with, for example, Pb values ranging from 0.01 to 0.25 nM in seawater and 1.20 to 2.11 nM in lake water. The results demonstrate the effectiveness of the AWA system in environmental studies that require the sampling of trace metals and nutrients without sample contamination., Peer reviewed

Plasmid maps and full sequences of Lit2C-PK2 and HomA-PK2; and plate images of plaque assay comparing P2 infection in ΔwaaC and BW25113 bacterial strains (−3 to −8)., Peer reviewed

Consellería do Medio Rural (Xunta de Galicia) supported the establishment of the trial network. Planting was carried out by Grupo Tragsa.Tree measurements were done by the MBG-CSIC under the project “RESILPINE” (RTI2018-094691-B-C33) funded by the Spanish Government., 1. Number of variables: 9 2. Number of cases/rows: 8400 3. Variable List: S X Y G ID B H SUR Section, Peer reviewed

Supplementary Table 1 | List of (A) transcriptomics, (B) metabolomics, (C) proteomics, and (D) ionomics studies retrieved from the literature., Peer reviewed

Sup1-Meeting-Full-agenda. Sup2-Survey1-results.xlsx Sup3-Survey2-Results.xlsx, Peer reviewed