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Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257586
Dataset. 2020

CAVEATS ON THE USE OF ROTENONE TO ESTIMATE MIXOTROPHIC GRAZING IN THE OCEANS [DATASET]

  • Duarte Ferreira, Guilherme
  • Calbet, Albert
Phagotrophic mixotrophs (mixoplankton) are now widely recognised as important members of food webs, but their role in the functioning of food webs is not yet fully understood. This is because of the lack of a well-established technique to estimate mixotrophy. An immediate step in this direction would be the development of a method that separates mixotrophic from heterotrophic grazing that can be routinely incorporated into the common techniques used to measure microplankton herbivory (e.g., the dilution technique). This idea was explored by the addition of rotenone, an inhibitor of the respiratory electron chain that has been widely used to selectively eliminate metazoans, both in the field and in the laboratory. Accordingly, rotenone was added to auto-, mixo-, and heterotrophic protist cultures in increasing concentrations (ca. 24 h). The results showed that mixotrophs survived better than heterotrophs at low concentrations of rotenone. Nevertheless, their predation was more affected, rendering rotenone unusable as a heterotrophic grazing deterrent. Additionally, it was found that rotenone had a differential effect depending on the growth phase of an autotrophic culture. Altogether, these results suggest that previous uses of rotenone in the field may have disrupted the planktonic food web, Marie Skłodowska-Curie grant agreement No 766327

Proyecto: EC/H2020/766327
DOI: http://hdl.handle.net/10261/257586
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257586
HANDLE: http://hdl.handle.net/10261/257586
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257586
PMID: http://hdl.handle.net/10261/257586
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257586
Ver en: http://hdl.handle.net/10261/257586
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257586

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257589
Dataset. 2021

MIXOPLANKTON INTERFERENCES IN DILUTION GRAZING EXPERIMENTS [DATASET]

  • Duarte Ferreira, Guilherme
  • Romano, Filomena
  • Medić, Nikola
  • Pitta, Paraskevi
  • Hansen, Per Juel
  • Flynn, Kevin J.
  • Mitra, Aditee
  • Calbet, Albert
It remains unclear as to how mixoplankton (coupled phototrophy and phagotrophy in one cell) affects the estimation of grazing rates obtained from the widely used dilution grazing technique. To address this issue, we prepared laboratory-controlled dilution experiments with known mixtures of phyto-, protozoo-, and mixoplankton, operated under different light regimes and species combinations. Our results evidenced that chlorophyll is an inadequate proxy for phytoplankton when mixoplankton are present. Conversely, species-specific cellular counts could assist (although not fully solve) in the integration of mixoplanktonic activity in a dilution experiment. Moreover, cell counts can expose prey selectivity patterns and intraguild interactions among grazers. Our results also demonstrated that whole community approaches mimic reality better than single-species laboratory experiments. We also confirmed that light is required for protozoo- and mixoplankton to correctly express their feeding activity, and that overall diurnal grazing is higher than nocturnal. Thus, we recommend that a detailed examination of initial and final plankton communities should become routine in dilution experiments, and that incubations should preferably be started at the beginning of both day and night periods. Finally, we hypothesize that in silico approaches may help disentangle the contribution of mixoplankton to the community grazing of a given system, Marie Skłodowska-Curie grant agreement No 766327. [...] With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)

Proyecto: EC/H2020/766327
DOI: http://hdl.handle.net/10261/257589
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257589
HANDLE: http://hdl.handle.net/10261/257589
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257589
PMID: http://hdl.handle.net/10261/257589
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257589
Ver en: http://hdl.handle.net/10261/257589
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257589

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257594
Dataset. 2022

THE STRENGTHS AND WEAKNESSES OF LIVE FLUORESCENTLY LABELLED ALGAE (LFLA) TO ESTIMATE HERBIVORY IN PROTOZOOPLANKTON AND MIXOPLANKTON [DATASET]

  • Duarte Ferreira, Guilherme
  • Figueira, Joana
  • Marques, Sónia Cotrim
  • Hansen, Per Juel
  • Calbet, Albert
The Live Fluorescently Labelled Algae (LFLA) technique has been used numerous times to estimate micro-zooplankton herbivory. Yet, it is unknown how mixoplankton (i.e., single-cell organisms that can combine phototrophy and phagotrophy) affect the outcome of this technique. Hence, we conducted a broad-spectrum assessment of the strengths and weaknesses of the LFLA technique, using several mixoplanktonic and proto-zooplanktonic grazers. Species from different taxonomic groups and different feeding mechanisms were tested in short-term experiments (ca. 5 h) in the laboratory, at different prey concentrations and during light and dark periods of the day. Overall, our findings suggest that the LFLA technique, due to its short-term nature, is an effective tracker of diel ingestion and digestion rates, and can detect new mixoplanktonic predators. We recommend that, irrespective of the prey concentration, incubations to measure grazing rates with this technique should generally be concluded within 1 h (adaptable to the environmental temperature). Nevertheless, our results also call for caution whenever using LFLA in the field: feeding mechanisms other than direct engulfment (like peduncle feeding) may provide severely biased ingestion rates. Furthermore, size and species selectivity are very hard to circumvent. To reduce the effects of selectivity, we propose the combined use of two distinctly coloured fluorochromes (i.e., distinct emission spectra). With this modification, one could either label different size ranges of prey or account for species-specific interactions in the food web, Marie Skłodowska-Curie grant agreement No 766327. [...] With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)

Proyecto: EC/H2020/766327
DOI: http://hdl.handle.net/10261/257594
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257594
HANDLE: http://hdl.handle.net/10261/257594
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257594
PMID: http://hdl.handle.net/10261/257594
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257594
Ver en: http://hdl.handle.net/10261/257594
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257594

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257598
Dataset. 2020

EFFECTS OF PREY TROPHIC MODE ON THE GROSS-GROWTH EFFICIENCY OF MARINE COPEPODS: THE CASE OF MIXOPLANKTON [DATASET]

  • Traboni, Claudia
  • Calbet, Albert
  • Saiz, Enric
Feeding rates, fecal production rates, egg production rates and gross-growth efficiencies of the copepod Paracartia grani offered a variety of phytoplankton, protozooplankton and mixoplankton in monodiet. See Excel file for details, This research was supported by EC MSCA-ITN 2019 funding to the project MixITiN (Grant Number 766327), Peer reviewed

Proyecto: EC/H2020/766327
DOI: http://hdl.handle.net/10261/257598
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257598
HANDLE: http://hdl.handle.net/10261/257598
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257598
PMID: http://hdl.handle.net/10261/257598
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257598
Ver en: http://hdl.handle.net/10261/257598
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257598

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257600
Dataset. 2021

FEEDING AND EGG PRODUCTION RATES OF THE COPEPODS PARACARTIA GRANI AND CENTROPAGES TYPICUS FEEDING ON THE DINOFLAGELLATE KARLODINIUM VENEFICUM UNDER DIFFERENT NUTRIENT AND TROPHIC CONDITIONS [DATASET]

  • Traboni, Claudia
  • Calbet, Albert
  • Saiz, Enric
Feeding and egg production rates of the copepods Paracartia grani and Centropages typicus feeding on the dinoflagellate Karlodinium veneficum under different nutrient and trophic conditions. See more details in the Excel file, This research was supported by EC MSCA-ITN 2019 funding to the project MixITiN (Grant Number 766327), Peer reviewed

Proyecto: EC/H2020/766327
DOI: http://hdl.handle.net/10261/257600
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257600
HANDLE: http://hdl.handle.net/10261/257600
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257600
PMID: http://hdl.handle.net/10261/257600
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257600
Ver en: http://hdl.handle.net/10261/257600
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257600

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257691
Dataset. 2021

LABORATORY GUIDELINES FOR THE DETECTION AND QUANTIFICATION OF PLASTICS PARTICLES FROM FRESHWATER ENVIRONMENTAL SAMPLES

  • Margenat, Henar
  • Cornejo, Delfina
  • Butler Margalef, Michael
  • Vila-Gispert, Anna
  • Guasch, Helena
The present document develops the laboratory protocols to analyse plastic pollution in freshwater environmental samples, although the methods followed can be extrapolated to other ecosystems. These protocols are developed in the framework of the PLASTICØPYR project, which aims to tackle plastic pollution associated with mountain tourism activities by developing transversal actions involving several stakeholders. These guidelines are developed in order to quantify plastic pollution in different river habitats, from large items (macro- and mesoplastics) to tiny plastic particles (microplastics). The protocols are developed for the analysis of microplastics (MicP) up to 63 µm, from samples from the water column, the fluvial sediments, the fluvial biota (i.e., brown trout and biofilm), as well as plastic particles present in the atmosphere (either wet or dry deposition). The MicP analysis is done following the Nile Red method, a fluorescent dye that facilitates the observation of small particles. Although Nile Red cannot provide chemical characterisation, it can be compatible with MicP identification techniques, such as FTIR (Fourier-transform infrared spectroscopy). The samples procedure includes: the extraction of MicP from the inorganic matrix by density separation (using ZnCl2), a digestion step with hydrogen peroxide and catalysed with Fenton's reagent to eliminate the organic remains and finally, the staining of the sample with Nile Red., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/257691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257691
HANDLE: http://hdl.handle.net/10261/257691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257691
PMID: http://hdl.handle.net/10261/257691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257691
Ver en: http://hdl.handle.net/10261/257691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257691

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257751
Dataset. 2022

PROPOSAL OF A LATE ASBIAN (MISSISSIPPIAN) STRATOTYPE FOR ENGLAND: TROWBARROW QUARRY, S. CUMBRIA, UK

  • Cózar, Pedro
  • Ian D. Somerville
  • Mark W. Hounslow
  • Tereza Kamenikova
Abstract: A detailed biostratigraphy is established, using the high foraminiferal abundance and diversity at Trowbarrow Quarry, which is also proposed as a stratotype section for the late Asbian (late Viséan), a substage not previously formally subdivided. The foraminiferal assemblages suggest that the early Asbian has to be revised, which has implications for the chronostratigraphy of many late Viséan outcrops in Britain, which are probably erroneously interpreted. From the upper part of the Park Limestone Formation at Trowbarrow Quarry, near the base of the section, the transition between foraminifers assigned to the early and late Asbian (Cf6α-β to Cf6γ subzones) is identified. The Urswick Limestone Formation contains excellent biostratigraphic records of many important taxa, allowing the subdivision of the formation into five foraminiferal assemblage zones for the late Asbian, namely Cf6γ1a, Cf6γ1b, Cf6γ2a, Cf6γ2b and Cf6γ2c. The last assemblage is currently only recognised in the North Pennines and at Trowbarrow. These assemblages allow revision of the stratigraphic range of many taxa used as biostratigraphic markers in Britain and Ireland. Owing to its excellent foraminiferal record, the late Asbian subdivisions will significantly facilitate the reinterpretation of other well-known Asbian platform carbonate successions in Britain. Although with some faunal differences, the base of the late Asbian can be confidently correlated to other European zonal schemes, with its base equivalent to the base of the former V3bγ or more recent MFZ14 foraminiferal zones of Belgium, and the base of the Mikhailovian Substage in Russia., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/257751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257751
HANDLE: http://hdl.handle.net/10261/257751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257751
PMID: http://hdl.handle.net/10261/257751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257751
Ver en: http://hdl.handle.net/10261/257751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/257751

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258356
Dataset. 2022

RNA-SEQ RAW DATA OF MAIZE INBRED BULKS RESISTANT AND SUSCEPTIBLE TO FUSARIUM VERTICILLIOIDES

  • Cao Caamaño, Ana
  • Fuente Martínez, María de la
  • Gesteiro Portas, Noemí
  • Santiago Carabelos, Rogelio
  • Malvar Pintos, Rosa Ana
  • Butrón Gómez, Ana María
[Data] Sequences of 150 bp cDNA paired-end recorded in FASTQ format and contained in files fq.gz. Two files for each sample because Sample1_1.fq.gz and Sample1_2.fq.gz contain read1 and read2 for paired-end sequencing of Sample1. More information in file Rawdata.readme.pdf [Files names ] A1_1.fq.gz: resistant inbred bulk, biological replicate 1, read1 A1_2.fq.gz: resistant inbred bulk, biological replicate 1, read2 A2_1.fq.gz: resistant inbred bulk, biological replicate 2, read1 A2_2.fq.gz: resistant inbred bulk, biological replicate 2, read2 A3_1.fq.gz: resistant inbred bulk, biological replicate 3, read1 A3_2.fq.gz: resistant inbred bulk, biological replicate 3, read2 A4_1.fq.gz: susceptible inbred bulk, biological replicate 1, read1 A4_2.fq.gz: susceptible inbred bulk, biological replicate 1, read2 A5_1.fq.gz: susceptible inbred bulk, biological replicate 2, read1 A5_2.fq.gz: susceptible inbred bulk, biological replicate 2, read2 A6_1.fq.gz: susceptible inbred bulk, biological replicate 3, read1 A6_2.fq.gz: susceptible inbred bulk, biological replicate 3, read2 Rawdata.readme.pdf AVAILABLE SOON, Fusarium verticillioides has been known as a causing agent of maize ear rot for long time, but the discovery of fumonisins, mycotoxins produced by F. verticillioides, increased concerns on kernel infection by F. verticillioides. A bulk-segregant RNA-seq approach has been used to identify differentially expressed genes (DEG) between bulks of four resistant and four susceptible inbreds to Fusarium ear rot (FER) and kernel fumonisin contamination in order to uncover the pathways involved in resistance. All inbreds were originated from the same F2 population. Plants were selfcrossed and 15 days later the main ear of the plant was inoculated. RNA-seq analysis was done with RNA extracted from kernels collected 10 days after inoculation using three biological replicates for each bulk (different plants of each inbred were used in each replicate). Bulk samples were barcoded and prepared for sequencing at Novogene Co., Ltd, where 150 bp paired-end (PE) reads were obtained on an Illumina 1.9., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/258356
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258356
HANDLE: http://hdl.handle.net/10261/258356
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258356
PMID: http://hdl.handle.net/10261/258356
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258356
Ver en: http://hdl.handle.net/10261/258356
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258356

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258395
Dataset. 2022

SIMULATED SEA SURFACE TEMPERATURE AND DERIVED SINGULARITY EXPONENTS FOR THE CALIFORNIA CURRENT SYSTEM (V.1.0) [DATASET]

  • Isern-Fontanet, Jordi
  • Capet, Xavier
  • Turiel, Antonio
  • Olmedo, Estrella
  • González-Haro, Cristina
Fundación General CSIC (Programa ComFuturo); TURBOMIX project (CGL2015-73100-EXP). With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), • lat: latitude • lon: longitude • x, x coordinate • y: y coordinate • time: time • sst: Sea Surface Temperature • hsst: Singularity exponents of Sea Surface Temperature, Peer reviewed

DOI: http://hdl.handle.net/10261/258395
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258395
HANDLE: http://hdl.handle.net/10261/258395
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258395
PMID: http://hdl.handle.net/10261/258395
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258395
Ver en: http://hdl.handle.net/10261/258395
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/258395

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/259323
Dataset. 2022

SEABIRD EGG VOLUME

  • Real, Enric
  • Oro, Daniel
  • Bertolero, Daniel
  • Igual, José Manuel
  • Sanz-Aguilar, Ana
  • Genovart, Meritxell
  • Hidalgo, Manuel
  • Tavecchia, Giacomo
The datasets contain measures of length and width of the egg of three species of Seabirds., Measures of egg volume of three seabirds: the Scopoli's shearwater Calonectis diomedea, the Sandwich tern Thalasseus sandvicensis and the Audouin’s gull Ichthyaetus audouinii in three colonies of the Western Mediterranean., No

DOI: http://hdl.handle.net/10261/259323
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/259323
HANDLE: http://hdl.handle.net/10261/259323
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/259323
PMID: http://hdl.handle.net/10261/259323
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/259323
Ver en: http://hdl.handle.net/10261/259323
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/259323

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