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Schematic representation of synteny conservation for p38 and p38/δ and plexin genes in animals, Peer reviewed

Supplementary Tables 1-7, Peer reviewed

The item contains Supplementary Files 1-4 for the manuscript "Evolutionary analysis of p38 stress activated kinases in unicellular relatives of animals suggest an ancestral function in osmotic stress", Peer reviewed

[Description of methods used for collection/generation of data] European wildcat scats were collected both systematically in fixed transects and opportunistically, between Dec 2018 and Feb 2020 in western Cantabrian Mountains and between Nov 2014 and April 2020 in Montaña Palentina Natural Park. For each sample we recorded the date and GPS location. Very old-looking scats were never collected. Scats were collected by experienced European wildcat researchers and assigned to European wildcats based on their morphology and smell. Although the certainty of assigning scats to carnivore species has been questioned (Monterroso et al., 2013), experimented researchers can accurately identify European wildcat scats. To assess the researchers’ success in scat identification, a subsample of 135 scats was genetically analysed (Ruiz-Villar et al. 2022). From 123 scats that we could genetically identify, 107 (87%) were from the Felis genus, 15 from Vulpes vulpes and one from Canis sp. The proportion of misidentified fox scats was the same in both study areas. Thus we were confident that Felis scats belong to wildcats considering that (1) wildcats and domestic cats segregate in the Cantabrian Mountains (Rodríguez et al., 2020), and we consequently avoided scat collection in areas used by domestic cats; and (2) no hybrids have been detected in the area so far (Tiesmeyer et al., 2020). To determine the prey species consumed by wildcats we used macroscopic identification of bones, teeth, scales, feathers and other identifiable prey remains extracted from washed scats. We determined the minimum number of individuals for each prey item in each scat based on the repetition of identifiable remains. We used the identification guide by Román (2019) to identify Spanish rodents from bone and teeth remains, and compared reptile and bird remains with specimens preserved in Doñana Biological Station. The study was undertaken with the correspondent permissions of the regional government of Castilla y León (EP/P/128/2019; AUTO/19/34, AUTO/2020/593) and Asturias (AUTO/2019/3267 for Asturias)., [EN] This dataset provides the raw results of wildcat, Felis silvestris, diet, by analyses of scats collected in the Cantabriam Mountains (mainly in Montaña Palentina Natural Park, Palencia, and in Western Cantabrian Mountains between the provinces of Asturias and León; Spain) between years 2014 and 2020. The dataset provides information of 683 scats and 3120 prey items, most them rodent species., [ES] Este conjunto de datos proporciona los resultados brutos de la dieta de gatos monteses, Felis silvestris, a través del análisis de excrementos recolectados en la Cordillera Cantábrica (principalmente en el Parque Natural Montaña Palentina, Palencia, y en el oeste de la Cordillera Cantábrica en las provincias de Asturias y León; España) entre los años 2014 y 2020. El conjunto de datos proporciona información de 683 excrementos y 3120 presas, la mayoría especies de roedores., This study was partially supported by M.Torres Diseños Industriales SAU and Jaguar Land Rover España., WildcatDiet_Cantabrian_Mountains.xlsx, Peer reviewed

Data and software for data analysis and to generate the respective figures., Peer reviewed

El libro de códigos está disponible en español y en traducción al inglés., [ES] La Noche Europea de los Investigadores es un evento de divulgación científica de alcance europeo que muestra de forma divertida e inspiradora la diversidad de la ciencia y su repercusión en la vida cotidiana de los ciudadanos. El evento es una iniciativa de la Comisión Europea y se financia a través de las Acciones Marie Sklodowska Curie (MSCA) del Programa Marco de Investigación e Innovación de la UE. La décima edición se celebró en 371 ciudades europeas, entre ellas las ocho capitales andaluzas el 24 de septiembre de 2021. En Andalucía, más de 80.000 personas participaron en 800 actividades desarrolladas por 2.700 investigadores. Este informe presenta datos significativos para medir el impacto de la Noche Europea de los Investigadores en Andalucía, así como la evaluación realizada por sus participantes: asistentes e investigadores., [EN] The European Researchers' Night is a Europe-wide scientific outreach event that displays the diversity of science and its impact on citizens' daily lives in fun, inspiring ways. The event is an initiative of the European Commission and is funded through the Marie Sklodowska Curie Actions (MSCA) of the EU’s Framework-Programme for Research and Innovation The tenth edition was celebrated in 371 European cities, including the eight Andalusian capitals on September 24, 2021. In Andalusia, over 80,000 people participated in 800 activities carried out by 2,700 researchers. This report presents significant data to measure the impact of the European Researchers' Night in Andalusia, as well as the evaluation conducted by its participants: attendees and researchers., Proyecto financiado por el Programa Marco de Investigación Horizon Europe de la Comisión Europea, en el marco de las acciones Marie Sktodowska-Curie., Peer reviewed

[Description of methods used for collection/generation of data] Polarimetric Radio Occultation measurement technique., The Polarimetric Radio Ocultation (PRO) Research Dataset (resPrf) provides the PRO observable Delta Phi as a function of height along with the corresponding standard RO wet thermodynamic profiles. The Delta Phi observable contained in this dataset is created from the processing of the H and V ports of the polarimetric antenna aboard PAZ, that provide information about precipitation and clouds encountered along the ray-paths. The thermodynamics is obtained from the merge of UCAR's atmPrf and wetPf2 level2 retrievals. The aim of the dataset is to foster the scientfic use of the PRO data, which provide unique simultaneous measurements of precipitation and the thermodynamics of the associated environment. In addition, the dataset provides detailed collocated information from independent precipitation sources, as well as radiances from the radiometers aboard the satellites in the Global Precipitation Mission (GPM) constellation. These collocated data are used for validation but also to provide the user with additional context on meteorological events., The creation of this dataset has been supported by the project PID2021-1264436OB-C22 funded by the MCIN/AEI (10.13039/501100011033) and the European Union “ERDF A way of making Europe”. Work performed at the ICE-CSIC was also partially supported by the program Unidad de Excelencia María de Maeztu CEX2020-001058-M., With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2020-001058-M)., One file per observation. Approximately between 100-200 observations per day. The individual files are packed in daily zipped tar files. Upon unpacking, the individual files are placed in a folder named after the date., Peer reviewed

[Description of methods used for collection/generation of data] Pooled-fecal microbiota from 5-7 years old chlidren was inoculated into the three colon reactors (R1, R2 and R3) of the BFBL gut simulator. The stabilized microbiota were inoculated (1%) with the child pooled fecal sample. After the microbiota stabilization stage, xylitol was added to the system at increasing concentrations (1 g/L, 3 g/L and 5 g/L), three times per day for 7 days for each concentration (samples X). The experiment without addition of xylitol to the BFBL gut simulator was carried out for the same period of time (Control, samples C). During the experimental set up, samples were collected every 24 h from the three colon reactors and centrifuged (11,200 ×g during 10 min at 4 °C), and the pellet was used for bacterial genomic DNA extraction (EZNA® Bacterial DNA Kit, Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). DNA samples were sent to Novogene Europe (Cambridge, UK) for sequencing the V3-V4 region of the 16S rRNA gene amplified by using 341F (5′- CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGA CTACNNGGGTATCTAAT-3′) primers. The PCR products were sequenced on a paired-end Illumina platform (NovaSeq 6000, Illumina) to generate 250 bp paired-end raw reads., MCIN/ AEI /10.13039/501100011033 (project reference: PID2019- 382 106071RB-I00)., Peer reviewed

Sensor: High Frequency Radar: CODAR SeaSonde system, 13.5 MHz; Data acquisition: High Frequency Radar; Time coverage: From 2nd March 2023 at 11.00AM; Time resolution: Hourly; Maps frequency generation: Hourly; Spatial coverage: Latitude range: 39.5851ºN-43.0681ºN Longitude range: 0.06352ºE-4.26898ºE; Spatial resolution: Latitude: 0.027 degrees North - Longitude: 0.03534 degrees East; Spatial grid: WGS 84 / Regular longitude-latitude, ICATMAR HF Radar Network has been established with the support of the European Maritime and Fisheries Fund, the European Maritime, Fisheries and Aquaculture Fund and the fund provided by the Government of Catalonia. The network has been designed, implemented and managed through the efforts of the Direcció General de Política Marítima i Pesca Sostenible (Government of Catalonia) and the Insitut de Ciències del Mar (CSIC), Barcelona, With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), Peer reviewed

Supplementary Figure S1. 1H-NMR spectrum of ascochlorin (1) (CDCl3, 400 MHz); Supplementary Figure S2. ESI/MS (+) spectrum of ascochlorin (1); Supplementary Figure S3. 1H-NMR spectrum of ascofuranol (2) (CDCl3, 400 MHz); Supplementary Figure S4. ESI/MS (+) spectrum of ascofuranol (2); Supplementary Figure S5. 1H-NMR spectrum of (R)-mevalonolactone (3) (CDCl3, 400 MHz); Supplementary Figure S6. ESI/MS (+) spectrum of (R)-mevalonolactone (3); Supplementary Figure S7. 1H-NMR spectrum of ascosalipyrone (4) (CDCl3, 400 MHz); Supplementary Figure S8. ESI/MS (+) spectrum of ascosalipyrone (4); Supplementary Figure S9. 1H-NMR spectrum of benzoic acid (5) (CDCl3, 400 MHz); Supplementary Figure S10. ESI/MS (+) spectrum of benzoic acid (5); Supplementary Figure S11. 1H-NMR spectrum of tyrosol (6) (CDCl3, 400 MHz); Supplementary Figure S12. ESI/MS (+) spectrum of tyrosol (6); Supplementary Figure S13. 1H-NMR spectrum of ascosalitoxin (7) (CDCl3, 400 MHz); Supplementary Figure S14. ESI/MS (+) spectrum of ascosalitoxin (7); Supplementary Figure S15. Images of the symptoms of each of the compounds. Supplementary Table S1. Diseased area (mm2) measured on detached leaves of several legume crops with exudates from the fungus A. fabae growth in vitro on 3 different culture media (Czapek-Dox = CD, potato dextrose broth = PDB, and rice) at concentrations of 0.5, 1, and 2 mg/mL. Negative (blank untreated, water and MeOH 5%) controls were also included. The experiment was repeated four times. Supplementary Table S2. Diseased area (mm2) measured in leaves detached from various legume crops with metabolites produced by the exudate of the A. fabae fungus from the three-growth media at concentrations of 1, 10, and 100 µM. Negative controls (untreated blank, water, and MeOH 5%) were also included. p-value compared with value from MeOH 5% control., Peer reviewed