BUSCADOR RECOLECTA

Analysis of pheromone content in T2 generation of SPv1.0 plants., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

Dataset are structured following well-established data formats. Three files are provided., The first file (Don_biom_red-swamp-crayfish_ev_20230111) contains the information of each event (eventID, event date, geographical coordinates, sample effort, etc…); the second file (Don_ biom_red-swamp-crayfish _occ_20230111) contains the information of the occurrences of individuals recorded in each station and its taxonomic classification; and the third file (Don_ biom_red-swamp-crayfish _mof_20230111) provide information of biometric variables of individual crayfish recorded (total body length, cephalothorax length, cephalothorax width and weight)., The monitoring of biometric parameters (total body length, cephalothorax length, cephalothorax width and weight) of red-swamp crayfish (Procambarus clarkii) in Doñana wetlands was initiated in 2004 as part of the Monitoring Program of Natural Resources and Processes. The aim was to obtain a temporal and continuous series of data in the biometry of the species to analyze the evolution in these variables. Data were recorded annually between 2004-2022 by more than 2 members of the monitoring team which performed samplings in different locations twice per year in winter-spring and summer seasons when the study sites are flooded. The individuals were sampled at the 64 stations classified according to their location (on either aeolian sands or marshland). Modified commercial traps, similar to funnel traps, with 4 mm mesh size were used as sampling method, following the method by Bravo et. al., 1994. Between 5-9 traps were randomly distributed (until 50 cm of depth) in each location, depending of the flooded area and depth. The traps were left for 24 hours and emptied the content into white sorting pans. Individuals were counted and identified until the maximun taxonomic level in the field and realease. Each individual captured was weigthed using an electronic balance within 0.2 g accuracy. Cephalothorax length (from the tip of the rostrum to the tip of the cephalatorax) and cephalothorax width (maximum width of the cephalothorax) were measured using a slide caliper within 0.1 mm accuracy. Total body length (from the tip of the rostrum to the tip of the telson) were recorded using a ruler within 0.5 mm accuracy. Data recorded during the surveys also included sex and maturation stage (inmature, mature) of the organisms when possible, as well as the time and georreferenced data of the capture. Between 2004-2007 data was registered in Excel file and since 2008 data was recorded in CyberTracker sequence. The protocol used has been supervised by researchers and the data have been validated by the members who performed the sampling. Bravo, M. A.; Duarte, C. M. & Montes, C. (1994) Environmental factors controlling the life history of Procambarus clarkii (Decapoda, Cambaridae) in a temporary marsh of the Doñana National Park (SW Spain). January 1994. Limnology 25(4):2450-2453. Romaire, R. P. & Lutz, C. G., 1989 Population dynamics of Procambarus clarkii (Girard) and Procambarus acutus acutus (Girard) (Decapoda: Cambaridae) in commercial ponds. Aquaculture 81(3-4):253-274., National Parks Autonomous Agency (OAPN) between 2002-2007; Singular Scientific and Technical Infrastructures from the Spanish Science and Innovation Ministry (ICTS-MICINN); Ministry of Agriculture, Livestock, Fisheries and Sustainable Development from the Regional Government of Andalusia (CAGPDES-JA) since 2007; and Doñana Biological Station from the Spanish National Research Council (EBD-CSIC) since all the study period (2004)., 1. Don_biom_red-swamp-crayfish_ev_20230111: eventID, intitutionCode, institutionID, datasetName, eventDate, eventTime, continent, country, stateProvince, location, localityID, locality, decimalLatitude, decimalLongitude, habitat, sampleSizeValue, sampleSizeUnit, sampleSizeEffort. 2. Don_ biom_red-swamp-crayfish _occ_20230111: eventID, occurrenceID, occurenceTime, individualCount, basisOfRecord, sex lifestage, kingdom, phylum, class, family, genus, specificEpithet, scientificName, scientificNameAuthorship. 3. Don_ biom_red-swamp-crayfish _mof_20230111: ocurrenceID, measurementID, measurementValue, measurementUnit, measurementType, measurementAccuracy, measurementMethod., Peer reviewed

The purpose of this assay is to define the volatilome of SxPv1.0, v1.2 and WT Nicotiana benthamiana plant roots, focusing on the differences between them, by GC-MS., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

The purpose of this assay is to define the volatilome of SxPv1.0, v1.2 and WT Nicotiana benthamiana plant leaves, focusing on the differences between them, by GC-MS., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

Analysis of pheromone content in T1 generation of SPv1.0 plants., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

The purpose of this study is to screen the population of T2 transgenic generation of SxPv1.2, to analyze the production of moth sex pheromones in these plants and be able to choose from them the most interesting ones in terms of production for further RNASeq analysis., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

The effect of EaDAct in pheromone production was studied by agroinfiltrating in Nicotiana benthamiana leaves the first two enzymes (AtrD11 and HarFAR) with or without the last enzyme EaDAct. P19 was also agroinfiltrated as negative control., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

Analysis of pheromone content in T3 generation of SPv1.0 plants, European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

Stable transformation of Nicotiana benthamiana plants with the three enzymes for moth pheromone production (AtrD11, HarFAR, EaDAct) expressed constitutively., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed

The constructs for SxPv1.1 and SxPv1.2 were tested transiently in Nicotiana benthamiana leaves. Pheromone content was analysed via GC-MS., European Commission: ERA CoBioTech - Cofund on Biotechnologies (722361)., Peer reviewed