Resultados de investigación

Additional file 1: Fig. S1. Validation of the pMLKL immunoreactivity by DAB staining. Representative micrographs of hippocampal brain areas of AD patients (n = 3) immunostained with anti-pMLKL antibody. Orange arrows indicate pMLKL-positive neurons, and green arrows show pMLKL-positive microglia. Magnification, 20X., Fondecyt, FONDAP, ISCIII, CIBERNED, Junta de Andalucia Consejería de Economía y Conocimiento, MICIN Ramon y Cajal Program National Institutes of Health., Peer reviewed

Additional file 2: Fig. S2. Coefficient of variation of the dot-blot assays. The coefficient of variation (CV) was calculated using triplicates of Braak V-VI samples. A CV of 11.63% and 15.06% was determined for OC and A11, respectively., Fondecyt FONDAP ISCIII CIBERNED Junta de Andalucia Consejería de Economía y Conocimiento MICIN Ramon y Cajal Program National Institutes of Health., Peer reviewed

Additional file 3: Fig. S3. Validation of the pMLKL antibody and assessment of cell specificity. Representative micrographs of (A) the dentate gyrus (DG) and (B) CA1 brain regions from wild-type and MLKL knockout mice subjected to intracerebral injection of Aβo, immunostained with anti-pMLKL antibody (scale bar, DG: 100 μm, CA1: 150 μm). (C, D) Representative images of the hilus of wild-type mice treated with Aβo, labeled with the indicated antibodies (scale bar, 50 μm and 25 μm for magnifications)., Fondecyt FONDAP ISCIII CIBERNED Junta de Andalucia Consejería de Economía y Conocimiento MICIN Ramon y Cajal Program National Institutes of Health., Peer reviewed

Additional file 4: Fig. S4. Intracerebral administration of Aβo elicits neurodegeneration in wild-type mice. Representative micrographs of brain sections from wild-type mice treated as indicated in the images, stained with Fluoro-Jade C (scale bar, 150 μm)., Fondecyt FONDAP ISCIII CIBERNED Junta de Andalucia Consejería de Economía y Conocimiento MICIN Ramon y Cajal Program National Institutes of Health., Peer reviewed

Additional file 5: Fig. S5. MLKL inhibition attenuates Aβo-induced microgliosis in mice. (A, B) Representative micrographs and quantitative analysis of brain sections from wild-type and Mlkl knockout mice treated as indicated in the images, immunostained with anti-Iba1 antibody (scale bar, 200 μm). The data are presented as mean ± S.E.M. and were analyzed by one-way ANOVA followed by Bonferroni post-test. *p < 0.05., Fondecyt FONDAP ISCIII CIBERNED Junta de Andalucia Consejería de Economía y Conocimiento MICIN Ramon y Cajal Program National Institutes of Health., Peer reviewed

Supplementary material to DESI's publication Constraining galaxy-halo connection with high-order statistics to comply with the data management plan. Including: Tabulated 2 point and 3 point counts of AbacusSummit highbase box at redshift 0.8, 1.1, and 1.4. HOD mock catalogs for LRGs at z=0.8, ELGs at z=0.8,1.1, QSOs at z=1.4 Jackknife Covariance from HOD mock catalogs MCMC chains., Peer reviewed

Data from figures 1 and 3, and a juypter notebook for plotting this data., Peer reviewed

Table_1_Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling. Table_2_Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling. Table_3_Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling. Table_4_Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling. Table_5_Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling. Table_6_Enhancing control systems of higher plant culture chambers via multilevel structural mechanistic modelling., Peer reviewed

Additional file 6: Fig. S6. RIPK3 inhibition does not alter Aβo-induced microgliosis in wild-type mice. (A, B) Representative micrographs and quantitative analysis of brain sections from wild-type mice treated as indicated in the images, immunostained with anti-Iba1 antibody (scale bar, 200 μm). The data are presented as mean ± S.E.M. and were analyzed by one-way ANOVA followed by Bonferroni post-test. *p < 0.05; **p < 0.01., Fondecyt FONDAP ISCIII CIBERNED Junta de Andalucia Consejería de Economía y Conocimiento MICIN Ramon y Cajal Program National Institutes of Health., Peer reviewed

Additional file 7: Fig. S7. Aβo neurotoxicity is worsened when microglial cells are present. (A) To characterize Aβo, a negative stain with 1% uranyl acetate was performed followed by electron microscopy analysis. Arrows indicate small oligomeric structures (scale bar, 200 nm). (B, C) Representative images and quantitative analysis of neurons treated as indicated, immunostained with anti-acetylated tubulin antibody (scale bar, 100 μm). (D, E) Representative micrographs and quantitative measurement of neurons and neuron/microglia cocultures treated as indicated, immunolabeled with anti-acetylated tubulin and anti-Iba1 antibodies as specified in the images (scale bar, 100 μm in the middle panel; 50 μm in the right panel). Each experiment was performed at least three independent times, with three replicates per condition each time. The data are presented as mean ± S.E.M. and were analyzed by one-way ANOVA followed by Bonferroni post-test. *p < 0.05; **p < 0.01; ****p < 0.0001., Fondecyt FONDAP ISCIII CIBERNED Junta de Andalucia Consejería de Economía y Conocimiento MICIN Ramon y Cajal Program National Institutes of Health., Peer reviewed