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[Description of methods used for collection/generation of data] ABR DATA: ABR recordings were performed on a TDT RZ6 evoked potential workstation (Tucker‐Davis Technologies, Alachua, FL, USA), as reported by Cediel et al. (2006). ABR test were performed before and after exposure to noise in G6PD transgenic and wild type mice. In brief, mice were anesthetized with ketamine (100 mg/kg; Imalgene 1000; Merial, Lyon, France) and xylazine (10 mg/kg; Rompun 2%; Bayer, Leverkusen, Germany) by intraperitoneal injection and the ABR tests were performed in a sound‐attenuating chamber. Two different sound stimuli, clicks and tone bursts, were generated with SigGenRP software (Tucker‐Davis Technologies). Stimuli were calibrated using SigCal software and an ACO Pacific 1⁄4‐inch microphone. Click stimuli were 0.1 ms and toneburst (4, 8, 16, 28, and 40 kHz) stimuli were 5‐ms duration (2.5 ms each for rise and decay, without plateau). The response was analyzed with BioSigRP software (Tucker‐Davis Technologies). Stainless steel needle electrodes were placed at the vertex and ventrolateral to the left and right ears for recording and a tweeter in open field configuration to deliver acoustic stimuli. Hearing thresholds were established at the lowest SPL level that produced a noticeable ABR five peaks wave and evoked a peak‐to‐peak voltage 2 SD above the mean background activity. Wave amplitudes, latencies, and inter‐wave latencies were determined at 70 dB SPL click stimulation. qPCR DATA: obtained by Real-Time PCR and analyzed by QuantStudio™ Real-Time PCR software 1.3. Cochleae were isolated from vestibules and frozen in RNAlater ® solution. Cochlear RNA extraction from pooled cochlea (n = 3 mice per experimental group), quality determination, and cDNA generation were performed as reported by Celaya et al. (2019). Quantitative amplification was performed in triplicate on an Applied Biosystems 7900HT Real‐Time PCR System using either commercial TaqMan probes or gene‐specific primers (Tables ​(Tables11 and ​and2).2). Data were collected after each amplification step and analyzed with SDS 2.2.2 software (Applied Biosystems, Foster City, CA, USA). The 18s gene was used as a housekeeping gene and the n‐fold differences were calculated using the 2–ΔΔCt method. Total G6PD expression levels are represented as the sum of the 2–ΔCt for the murine and human primers. WB data: Whole cochleae protein extracts were prepared from 3 mice as described (Sanchez‐Calderon et al., 2010). An equal volume of extracts was resolved using SDS‐PAGE, followed by transfer to PVDF membranes (0.2 μm; Bio‐Rad Laboratories, Hercules, CA, USA) using the Bio‐Rad Trans Blot TURBO apparatus. Membranes were blocked with 5% BSA or non‐fat dried milk in 0.075% Tween, 1 mM TBS, and incubated overnight with the following antibodies: HO-1 1:1000 Conejo Pc Millipore/ #AB1284 SOD2 1:1000 Conejo Pc Millipore/ #06-984 NQO1 1:1000 Cabra Pc Abcam/ #ab2346 NRF2 1:1000 Conejo Pc No comercial p38 1:2000 Conejo Pc Santa Cruz/ #sc-535 p-p38 1:1000 Conejo Pc Cell Signaling/ #9211 Vinculina 1:15000 Ratón Mc Santa Cruz/ #sc-73614 IgG de cabra 1:5000 Conejo Bio-Rad Laboratories/ #1721034 IgG de conejo 1:3000 Cabra Bio-Rad Laboratories/ #1706515 IgG de ratón 1:3000 Cabra Bio-Rad Laboratories/ #1706516 Membranes were then incubated with a peroxidase‐conjugated secondary antibody for 1 h at room temperature, and bands were visualized using Clarity™ Western ECL Substrate (Bio‐Rad) using an ImageQuant LAS4000 mini digital camera (GE Healthcare Bio‐Sciences, Pittsburgh, PA, USA) and densities were quantified using Image Quant TL software. ENZYMATIC ACTIVITY DATA: The measurement of the enzyme activity of G6PD, 6PGD and SOD was performed in mitochondrial or cytosolic extracts. Samples were manually homogenized in a buffer extraction. The lysates were centrifuged to obtain the nuclear fraction (precipitated). The supernatants were centrifuged to obtain the cytosolic (supernatant) fraction. The mitochondrial fraction was obtained by homogenizing the precipitated. The homogenized precipitate is Incubated in the extraction buffer for 30 minutes on ice and centrifuged at 13,000 rpm for 10 minutes at 4ºC. Concentration protein of each of the cell fractions was determined using the DCTM Protein kit Assay Kit II (Bio-Rad Laboratories). The activity of the enzymes G6PD and 6PGD was determined in the cytosolic fractions, as reported (White et al., 2017). The enzymatic activity of SOD was determined in the cytosolic and using the EpiQuikTM Superoxide Dismutase Activity/Inhibition Assay Kit (EpiGentek). Optical density was measured with the VERSAmaxTM spectrophotometer Tunable Microplate Reader using SOFTmax® Pro 3.0 Software, THEARPY: bases genéticas y moleculares de la sordera neurosensorial y del daño auditivo: exploración de nuevas dianas y estrategias terapéuticas”. Convocatoria 2020 Proyectos de I+D+i - RTI Tipo B (PID2020-115274RB-I00). FEDER/MICIN 266.200€, 2021-2024. IPs: Isabel Varela Nieto (IVN) & Silvia Murillo Cuesta, CSIC., 1) ABR DATA RAW DATA (.arf files obtained along the experiment with the Tucker Davis Tecnologies (TDT) Auditory potential workstation). RESULTS (txt, excel and SPSS files with the data obtained after ABR register analysis). 2) qPCR DATA RAW DATA (excel and jpg files obtained from the qPCR equipment) RESULTS (Analysis SYBR Cloud, Analysis TQ Cloud, Dissociation curves) 3) WB DATA RAW DATA (TIFF files of HO1, NQO1, NRF2, P22PHOX, p38, p-p38 p-JNK and SOD2 Western blotting detection). WB REPORTS (PDF files with the reports obtained with ImageQuantTM TL (GE Healthcare) software. RESULTS (Excel file with WB quantification, SPSS files with statistic analysis). 4) ENZYMATIC ACTIVITY DATA RAW DATA (Excel file with results of G6PD, 6PGD and SOD enzymatic activity). ANALYSIS (SPSS and PDF files with statistic analysis), Peer reviewed

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Este conjunto de datos está sujeto a una licencia CC BY-SA 4.0, [EN] Dataset obtained from the CSIC's Library & Archive Network, which includes the digitalized documents from the collection “Manuscritos” (“Manuscripts”) preserved at the Tomás Navarro Tomás library belonging to the Centro de Ciencias Humanas y Sociales (CCHS) of the CSIC. The now disappeared Centro de Estudios Históricos (CEH) compiled a collection of miscellaneous documents. The complete collection contains 195 documents from private archives that were bound and gathered in codices and dossiers. It comprises mainly notary documents, testaments, property titles, papers related to administration and property management and accounting books, but it also includes work of a historical and literary character. This V1 of the dataset contains the 135 documents that have been digitalized to date., [ES] Dataset procedente del catálogo de la Red de Bibliotecas y Archivos del CSIC que recoge los documentos digitalizados del fondo “Manuscritos” conservados en el Archivo de la biblioteca Tomás Navarro Tomás del Centro de Ciencias Humanas y Sociales (CCHS) del CSIC. Se trata de una colección de documentos misceláneos reunidos durante la etapa del Centro de Estudios Históricos (CEH). La colección al completo está formada por 195 documentos agrupados en legajos y códices encuadernados procedentes de archivos privados. Predomina la documentación de carácter notarial, testamentos y títulos de propiedad, de administración y gestión de propiedades, los libros de cuentas pero también hay obras de carácter histórico y literario. Este dataset contiene los 135 documentos digitalizados hasta el momento., No

4 pages. -- Supplemental Figure S1: ER localization of B117L upon expression in HeLa cells. -- Supplemental Figure S2: OSER formation in cells expressing the B117L gene. -- Supplemental Figure S3: Effects of N-terminal deletions., Peer reviewed

This Electronic Supporting Material contains details about the Experimental section as well as some additional comments about Results and Discussion section. The Experimental section describes the reagents employed for the synthesis and characterization of the IrNCs and IrNCs-immunoprobes, the culture and incubation of ARPE-19 cells, the immunocytochemistry (ICC) assays for the detection of target proteins by MS and fluorescence, the cellular preparation for sc-ICP-MS and LA-ICP-MS, as well as the ELISA analyses. Furthermore, protocols for the synthesis and characterization of IrNCs and IrNCs-immunoprobes are also included. In the Results and Discussion section, results regarding the optimization of the glucose-induced oxidative stress treatment, the determination of proteins in individual ARPE-19 cells by sc-ICP-MS, as well as the bioimaging of APOE and claudin-1 in individual ARPE-19 cells by LA-ICP-MS are also collected., Peer reviewed

ESM contains details about the Results and Discussion section. Transmission electron microscopy (TEM) images of extracellular vesicles (EVs) isolated are shown. Furthermore, a graph with the Fe, Cu and Zn concentrations determined by ICP-MS in exosomes from control and treated HRPEsv cells culture medium are also depicted, Peer reviewed

Features of Enterococcus faecium bacteriophage vB_EfaH_163. The orfs, gene number and gene position in the vB_EfaH_163 genome are shown, as are the predicted functions, molecular weights and isoelectric points of the encoded products. The predicted functions were assessed using RAST and PATRIC software. The top BLAST hit and E-values are also indicated., Peer reviewed

Dataset are structured following well-established data formats. Two files are provided and they are related to each other with the variable eventID. The first file (icts-rbd-GenettaTracks_ev_20230915) contains the information of each event (time of occurrence, geographical coordinates or sampling effort); the second file (icts-rbd-GenettaTracks_occ_20230915) contains the count of tracks for each species recorded in each site, numbers of tracks recorded and taxonomic classification., [Description of methods used for collection/generation of data] The long-term monitoring of carnivore tracks in Doñana is part of a harmonised protocol for the Long-term Ecological Monitoring Program of Natural Resources and Processes targeting mammals' populations. The general aim of this protocol is to study the temporal evolution of the relative density of the main species of carnivores in the main habitats of the Doñana National Park. Tracks surveys were done annually after the first rains of the hydrological year, i.e. the first autumn rains, usually in October. Due to climate change, in recent years the rainy season has been delayed until the beginning of the year. This protocol has stablished in 2007 and it has done annually until the present (2022), except in 2021 when due to logistical problems no census was made. Censuses are carried out through 12 prefixed transect, with sand substrate, in Doñana National Park. Each transect consists of a 2 km of length and 1.5 m of width that is done by a car at a constant speed between 10 and 15 km/h. Transects are cleaned the day before of the census with a metal beam to facilitate the read of the tracks and to ensure that the foot prints were from the previous day. Each transect is repeated in three consecutive days, and during the transect the sand is cleaned for the next day. In the census an expert in mammals’ tracks identifies all the tracks, i.e. groups of carnivore foot prints, and he/she records them in Cybertracker. That way, tracks' information like coordinates, hour, species identification and observation was recorded; and also the information of each transect was recorded: sampler, drivers, date, start and end (hour and coordinates). This method enables to calculate Kilometric Abundance Indexes (KAI) for each species and transect. In order to clarify all carnivore datasets, the data was separated by species, this allows concrete analysis by species. In this dataset common genet ´s (Genetta genetta) data is presented., [Methods for processing the data] The data was recorded in CyberTracker sequence. The protocol used has been supervised by researchers and the data have been validated by the members who performed the sampling. The raw data was processed with Excel., The long-term monitoring of carnivore tracks in Doñana is part of a harmonised protocol for the Long-term Ecological Monitoring Program of Natural Resources and Processes targeting mammals' populations. The general aim of this protocol is to study the temporal evolution of the relative density of the main species of carnivores in the main habitats of the Doñana National Park. Tracks surveys were done annually after the first rains of the hydrological year, i.e. the first autumn rains, usually in October. Due to climate change, in recent years the rainy season has been delayed until the beginning of the year. This protocol has stablished in 2007 and it has done annually until the present (2022), except in 2021 when due to logistical problems no census was made. Censuses are carried out through 12 prefixed transect, with sand substrate, in Doñana National Park. Each transect consists of a 2 km of length and 1.5 m of width that is done by a car at a constant speed between 10 and 15 km/h. Transects are cleaned the day before of the census with a metal beam to facilitate the read of the tracks and to ensure that the foot prints were from the previous day. Each transect is repeated in three consecutive days, and during the transect the sand is cleaned for the next day. In the census an expert in mammals’ tracks identifies all the tracks, i.e. groups of carnivore foot prints, and he/she records them in Cybertracker. That way, tracks' information like coordinates, hour, species identification and observation was recorded; and also the information of each transect was recorded: sampler, drivers, date, start and end (hour and coordinates). This method enables to calculate Kilometric Abundance Indexes (KAI) for each species and transect. In order to clarify all carnivore datasets, the data was separated by species, this allows concrete analysis by species. In this dataset common genet´s (Genetta genetta) data is presented., We acknowledge financial support from National Parks Autonomous Agency (OAPN) in 2007; the Singular Scientific and Technical Infrastructures from the Spanish Science and Innovation Ministry (ICTS-MICINN); the Ministry of Agriculture, Livestock, Fisheries and Sustainable Development from the Regional Government of Andalusia (CAGPDES-JA) since 2011; the Doñana Biological Station from the Spanish National Research Council (EBD-CSIC) since all the study period (2011); the Ministry of Environmetal sustainability and blue economy from the Regional Goverment of Andalusia since 2017 with the LIFE-ADAPTAMED project; Ministry of Science and Innovation (Recovery, Transformation and Resilence Plan); and the European Comision with the Long-term Ecosystem Research in Europe (eLTER) (a HORIZON funding coordination of the European funding programme for research and innovation) and NextGenerationEU funding., 1. icts-rbd-GenettaTracks_ev_20230915: eventID, institutionCode, institutionID, datasetName, eventDate, year, month, day, verbatimEventDate, eventTime, country, continent, countryCode, stateProvince, county, municipality, locality, verbatimLocality, verbatimCoordinates, geodeticDatum, samplingProtocol, SampleSizeValue, sampleSizeUnit, samplingEffort and eventRemarks. 2. icts-rbd-GenettaTracks_occ_20230915: eventID, occurrenceID, collectionCode, decimalLatitude, decimalLongitude, dynamicProperties, basisOfRecord, recordedBy, occurrenceStatus, individualCount, identifiedBy, scientificName, kingdom, phylum, class, order, family, genus, specificEpithet, scientificNameAuthorship, taxonRank, organismQuantity, organismQuantityType and occurrenceRemarks., Peer reviewed

Dataset are structured following well-established data formats. Two files are provided and they are related to each other with the variable eventID. The first file (icts-rbd-MelesTracks_ev_20230915) contains the information of each event (time of occurrence, geographical coordinates or sampling effort); the second file (icts-rbd-MelesTracks_occ_20230915) contains the count of tracks for each species recorded in each site, numbers of tracks recorded and taxonomic classification., [Description of methods used for collection/generation of data] The long-term monitoring of carnivore tracks in Doñana is part of a harmonised protocol for the Long-term Ecological Monitoring Program of Natural Resources and Processes targeting mammals' populations. The general aim of this protocol is to study the temporal evolution of the relative density of the main species of carnivores in the main habitats of the Doñana National Park. Tracks surveys were done annually after the first rains of the hydrological year, i.e. the first autumn rains, usually in October. Due to climate change, in recent years the rainy season has been delayed until the beginning of the year. This protocol has stablished in 2007 and it has done annually until the present (2022), except in 2021 when due to logistical problems no census was made. Censuses are carried out through 12 prefixed transect, with sand substrate, in Doñana National Park. Each transect consists of a 2 km of length and 1.5 m of width that is done by a car at a constant speed between 10 and 15 km/h. Transects are cleaned the day before of the census with a metal beam to facilitate the read of the tracks and to ensure that the foot prints were from the previous day. Each transect is repeated in three consecutive days, and during the transect the sand is cleaned for the next day. In the census an expert in mammals’ tracks identifies all the tracks, i.e. groups of carnivore foot prints, and he/she records them in Cybertracker. That way, tracks' information like coordinates, hour, species identification and observation was recorded; and also the information of each transect was recorded: sampler, drivers, date, start and end (hour and coordinates). This method enables to calculate Kilometric Abundance Indexes (KAI) for each species and transect. In order to clarify all carnivore datasets, the data was separated by species, this allows concrete analysis by species. In this dataset European badger ´s (Meles meles)data is presented., Methods for processing the data: The data was recorded in CyberTracker sequence. The protocol used has been supervised by researchers and the data have been validated by the members who performed the sampling. The raw data was processed with Excel., The long-term monitoring of carnivore tracks in Doñana is part of a harmonised protocol for the Long-term Ecological Monitoring Program of Natural Resources and Processes targeting mammals' populations. The general aim of this protocol is to study the temporal evolution of the relative density of the main species of carnivores in the main habitats of the Doñana National Park. Tracks surveys were done annually after the first rains of the hydrological year, i.e. the first autumn rains, usually in October. Due to climate change, in recent years the rainy season has been delayed until the beginning of the year. This protocol has stablished in 2007 and it has done annually until the present (2022), except in 2021 when due to logistical problems no census was made. Censuses are carried out through 12 prefixed transect, with sand substrate, in Doñana National Park. Each transect consists of a 2 km of length and 1.5 m of width that is done by a car at a constant speed between 10 and 15 km/h. Transects are cleaned the day before of the census with a metal beam to facilitate the read of the tracks and to ensure that the foot prints were from the previous day. Each transect is repeated in three consecutive days, and during the transect the sand is cleaned for the next day. In the census an expert in mammals’ tracks identifies all the tracks, i.e. groups of carnivore foot prints, and he/she records them in Cybertracker. That way, tracks' information like coordinates, hour, species identification and observation was recorded; and also the information of each transect was recorded: sampler, drivers, date, start and end (hour and coordinates). This method enables to calculate Kilometric Abundance Indexes (KAI) for each species and transect. In order to clarify all carnivore datasets, the data was separated by species, this allows concrete analysis by species. In this dataset European badger´s (Meles meles) data is presented., We acknowledge financial support from National Parks Autonomous Agency (OAPN) in 2007; the Singular Scientific and Technical Infrastructures from the Spanish Science and Innovation Ministry (ICTS-MICINN); the Ministry of Agriculture, Livestock, Fisheries and Sustainable Development from the Regional Government of Andalusia (CAGPDES-JA) since 2011; the Doñana Biological Station from the Spanish National Research Council (EBD-CSIC) since all the study period (2011); the Ministry of Environmetal sustainability and blue economy from the Regional Goverment of Andalusia since 2017 with the LIFE-ADAPTAMED project; Ministry of Science and Innovation (Recovery, Transformation and Resilence Plan); and the European Comision. with the Long-term Ecosystem Research in Europe (eLTER) (a HORIZON funding coordination of the European funding programme for research and innovation) and NextGenerationEU funding., 1. icts-rbd-MelesTracks_ev_20230915: eventID, institutionCode, institutionID, datasetName, eventDate, year, month, day, verbatimEventDate, eventTime, country, continent, countryCode, stateProvince, county, municipality, locality, verbatimLocality, verbatimCoordinates, geodeticDatum, samplingProtocol, SampleSizeValue, sampleSizeUnit, samplingEffort and eventRemarks. 2. icts-rbd-MelesTracks_occ_20230915: eventID, occurrenceID, collectionCode, decimalLatitude, decimalLongitude, dynamicProperties, basisOfRecord, recordedBy, occurrenceStatus, individualCount, identifiedBy, scientificName, verbatimScientificName, kingdom, phylum, class, order, family, genus, specificEpithet, scientificNameAuthorship, taxonRank, organismQuantity, organismQuantityType and occurrenceRemarks., Peer reviewed