Resultados de investigación

Table S1 Concentrations (ng/g) of organochlorine compounds in tissue (blood and adipose) of caracals in the Greater Cape Town area, South Africa in terms of wet weight (w.w.) and lipid weight (l.w.). Table S2 Spatial covariates considered for models of organochlorine exposure in caracal in the Greater Cape Town area, South Africa. Fig. S3 Boxplots (diamonds = means) of organochlorine compound concentrations (ng/g, logged) in whole blood and adipose of caracals in the Greater Cape Town area, South Africa. Table S4 Estimates (SE) of top linear mixed effects models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and spatial predictors in caracal in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S5 Estimates (SE) of top linear mixed effects models (based on AICc) of adipose organochlorine levels (ng/g, logged) and spatial predictors in caracal in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S6 Diet proportions in several prey groupings for caracals around Cape Town, South Africa as determined by through integrating diet datasets from scat and prey remains found at GPS clusters. Table S7 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (biomass) for broad taxonomic groups for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S8 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (FO) for broad taxonomic groups for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S9 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (biomass) for prey functional groups for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S10 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (FO) for prey functional groups for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S11 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (biomass) for prey foraging habitat for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S12 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (FO) for prey foraging habitat for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S13 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (biomass) for exotic prey groups for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S14 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and diet (FO) for exotic prey groups for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S15 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and blood cell count for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S16 Estimates (SE) for top models (based on AICc) of whole blood organochlorine levels (ng/g, logged) and serum biochemistry measures for caracals in the Greater Cape Town area, South Africa (*P < 0.1; **P < 0.05; ***P < 0.01). Table S17 Comparison of DDT concentrations (ng/g) ± SE in wild terrestrial mammalian predators from published studies. Where possible, like tissue was compared with like, but where not, serum/plasma was compared with caracal whole blood and liver/kidney was compared with caracal adipose. Table S18 Comparison of PCB concentrations (ng/g) ± SE in wild terrestrial mammalian predators from published studies. Where possible, like tissue was compared with like, but where not, serum/plasma was compared with caracal whole blood and liver/kidney was compared with caracal adipose., Peer reviewed

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13 pages. -- The file includes: Chemical analysis of pesticides. -- Table S1. Mass spectrometer parameters for 2,4-D and fipronil. -- Quality assurance/Quality control. -- Table S2. Linear range, limit of quantification (LOQ) and determination coefficient (R2) for 2,4-D and fipronil using external calibration method. -- Table S2. Linear range, limit of quantification (LOQ) and determination coefficient (R2) for 2,4-D and fipronil using external calibration method. -- Figure S1. Overview of system (Heterogeneous Multi-Habitat Test System - HeMHAS) used in the present study, featuring the compartment, the piece that connect the compartments and the gyratory door. -- Table S4: Values (mean ± standard deviation) of fipronil and 2,4-D determined at each concentration tested in the avoidance experiments to insecticide and herbicide, respectively, in micrograms per liter (μg/L). -- Table S5: Values (mean ± standard deviation) of fipronil and 2,4-D, determined at each concentration tested in the avoidance experiment to herbicide and insecticide, respectively, in micrograms per liter (μg/L). -- Table S6a: Mixed-design ANOVA for avoidance test with D. rerio exposed to fipronil at 20 °C. -- Table S6b:4Within-subjects effects with Greenhouse-Geisser correction for degrees of freedom. -- Table S6c:5Between-subjects effects. -- Table S7a:6Mixed-design ANOVA for avoidance test with D. rerio exposed to fipronil at 24 °C. -- Table S7b:7Within-subjects effects. -- Table S7c:8Between-subjects effects. -- Table S8a:9Mixed-design ANOVA for avoidance test with D. rerio exposed to fipronil at 28 °C. Table S8b:10Within-subjects effects. -- Table S8c:11Between-subjects effects. -- Table S9a:12Mixed-design ANOVA for avoidance test with D. rerio exposed to 2,4-D at 20 °C. -- Table S9b:13Within-subjects effects. -- Table S9c:14Between-subjects effects. -- Table S10a:15Mixed-design ANOVA for avoidance test with D. rerio exposed to 2,4-D at 24 °C. -- Table S10b:16Within-subjects effects. -- Table S10c:17Between-subjects effects. -- Table S11a:18Mixed-design ANOVA for avoidance test with D. rerio exposed to 2,4-D at 28 °C. -- Table S11b:19Within-subjects effects with Greenhouse-Geisser correction for degrees of freedom. -- Table S11c:20Between-subjects effects. -- Table S12:21Final results (mean ± standard error of the percentages of fish) for the avoidance experiments per zones - pesticide mixtures at different air temperatures (20, 24 and 28 degrees)., Peer reviewed

2 pages. -- Supplementary figure 1: Distribution of D. rerio juveniles along the gradients of Glyphosate, Chlorpyrifos and Chlorothalonil, throughout the three-hour period of exposure to the gradient of each substance individually. -- Supplementary figure 2. Distribution of D. rerio juveniles along the gradients of a mixture of Glyphosate-Chlorpyrifos, and Glyphosate-Chlorothalonil, throughout the three-hour period of exposure in the open gradient of each mixture., Peer reviewed

59 pages. -- Contents: Point S1. Gaussian keywords used in the calculations. -- Figure S1. Experimental spectra of PFPG+g+/G-g- and its isotopologues species. -- Table S1-S7. Transition frequencies of PFPG+g+/G-g- and its isotopologues and PFPTg+/Tg-. -- Figure S2. Experimental spectra of the PFPTg+/Tg- monomer. -- Figure S3. Conformational interconversion barrier of the PFP conformers. -- Table S8-S10. Kraitchamn and STRFIT results of PFPG+g+/G-g. -- Table S11-S13. The semi-experimental equilibrium structural parameters of PFPG+g+/G-g-. -- Table S14. Spectroscopic properties of the predicted binary PFP conformers. -- Table S15-S19. Rotational transition frequencies of the five binary PFP conformers. -- Figure S4. QTAIM and NCI analyses of the five PFP and PrOH conformers. -- Figure S5. QTAIM and NCI analyses of the five binary PFP conformers. -- Completion of reference 24., Peer reviewed

29 pages. -- PDF file includes: Strong-field coherence breaking (SFCB) details; Internal rotation methyl barrier of 4-methyl guaiacol (MG). -- Figure S1: Experimental and simulated variation of the internal rotational barrier of methyl group. -- Figure S2: Summary of bond changes of lignin relative to the average bond length rC-C. -- Figure S3: Optimized structure of guaiacol at B3LYP-D3BJ/def2tzvp level of theory. -- Table S1: Experimental and calculated constants derived from the broadband rotational spectrum of guaiacol. -- Table S2: List of spectroscopic parameters used to fit guaiacol isotopomers. -- Table S3: Experimental and calculated constants derived from the broadband rotational spectrum of syringol. -- Table S4: Molecular parameters of 4-methyl guaiacol. -- Table S5: Molecular parameters for Z- and E-4-vinyl guaiacol. -- Table S6: Line list of transitions fitted for guaiacol (G). -- Table S8: Line list of transitions fitted for 13C(2) guaiacol. -- Table S9: Line list of transitions fitted for 13C(3) guaiacol. -- Table S10: Line list of transitions fitted for 13C(4) guaiacol. -- Table S11: Line list of transitions fitted for 13C(5) guaiacol. -- Table S12: Line list of transitions fitted for 13C(6) guaiacol. -- Table S13: Line list of transitions fitted for 13C(7) guaiacol. -- Table S14: Line list of transitions fitted for syringol (S). -- Table S15: Line list of transitions fitted for methyl guaiacol (MG). -- Table S16: Line list of transitions fitted for vinyl guaiacol (Z-VG). -- Table S17: Line list of transitions fitted for vinyl guaiacol (E-VG)., Peer reviewed

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S1 Table: Top table of the differential expressed proteins between AF and no AF. Results from the discovery study., Results from the discovery study. Proteins selected to be verified in the whole Phase 1 are highlighted in grey. Proteins in bold but not highlighted were already tested in the whole phase 1 as part of a previous published work., Peer reviewed

13 pages. -- PDF file includes: 1. Supplementary Methods: 1.1. LDL Acetylation; 1.1. Quantitative and Qualitative Analysis of Foam Cell Formation; 1.2. Immunofluorescent Imaging of Co-culture Inserts; 1.4. Western Blot Analysis. -- Supplementary Figure 1. miRNA transfer capacity by DPPC:CE:LPC rHDL to foam cells cultured in monolayer. -- Supplementary Figure 2. Combination of TO901317 and antagomiR-33a-rHDL caused a synergistic upregulation of ABCA1 and ABCG1 protein levels in foam cells. -- Supplementary Figure 3. Intracellular Cholesterol Levels in Foam Cells After TO901317 Treatment, AntagomiR-33a-rHDL Delivery or Combined Treatment of TO901317 and AntagomiR-33a-rHDL in 1D Grown Foam Cells or 2D Atheroma Model Foam Cells. -- Supplementary Figure 4. Cholesterol efflux promoted in foam cells by sequential rHDL administration in a triple cell 2D atheroma model. -- Supplementary Figure 5. Agarose gel electrophoresis of acetylated LDL (LDLac). -- Supplementary Figure 6. Quantitative and Qualitative Analysis of Foam Cell Formation. -- Supplementary Figure 7. Development of triple-compartment cell culture 2D atheroma model. -- Supplementary Figure 8. Timeline showing cholesterol efflux experimental design., Peer reviewed

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