BUSCADOR RECOLECTA

[Measurement details] In static measurements, the whole batch dispersion, having well-defined density and deflocculant ratio, was gently stirred with a spoon for approximately 1 min, and a fraction of it was transferred to a 50-mL vial. The measurement probe was inserted in the fluid, following instructions from the respective providers. In dynamic measurements, 3 batches of the clay dispersion (ρ = 1.55 g·cm-3, deflocculant ratio 0.44 wt.%) were added together into a 2 L rectangular hermetic container. Prior to measurements, the dispersion was gently mixed with a spoon. Both the viscometer probe and the stirring rotor, operating at 1,000 rpm, were simultaneously located at opposite sides of the container. In the casting experiments, two different products were used: i) the Euroarce sanitary slip (ρ = 1.96 g·cm-3); and ii) an aqueous dilution of that sanitary slip, resulting in a density of ρ = 1.81 g·cm-3. The slip was hand stirred with a spoon and transferred into a plaster mould through a filling hole. The plaster mould, used to conform bar specimens measuring 12 x 3 x 1.5 cm3, consists of two symmetrical components joined together with adhesive tape. For measurement purposes, the VRV probe was introduced in the filled mould through the filling hole.-- Under a Creative Commons license BY-NC-ND 4.0., [Materials and equipment] Industrias Químicas del Ebro (Zaragoza, Spain) supplied the deflocculant solution of sodium silicate (3.3 SiO2·Na2O, 43% dry mass, NASIL 3.35). Elemental analysis was acquired from Energy-dispersive X-ray spectroscopy (EDX) in a SEM-EDX Hitachi S-3400 N, equipped with a Si EDX analyser Röntec XFlash. Crystal structure was assessed by powder X-ray diffraction (XRD) in a Bruker AXS D8 Advance diffractometer. Electrolytic conductivity was measured using an Orion Star A112, with a 2-electrode probe, reference 011050MD, which had a nominal cell constant of 1.0 cm-1 and a measurement range from 1 μS·cm-1 to 20 mS·cm-1. The flow curve was determined in a Rotovisco Rheometer from Thermo Scientific. The rotatory viscometer was a Brookfield DV-E (Brookfield Engineering, Middleboro, Massachusetts, USA), using LV3 and LV4 spindles, and operating at 30 rpm.-- [Preparation of clay dispersions] Dispersions were prepared from the raw clay at two concentrations, resulting in different final densities, and various ratios of deflocculant sodium silicate.-- [Chemical composition and mineralogical characterization] Both elemental analysis by EDX and the XRD pattern confirmed that the raw clay consisted of a mixture of alumiosilicate minerals.-- [Flow curve] The general flow characteristics of a control Euroarce clay dispersion, performed with a rotational rheometer, revealed the characteristic non-Newtonian shear-thinning behavior, where viscosity (η) strongly decreased with stirring shear rate (𝛾̇) (Figure S2) (Amorós et al., 2002; Amorós et al., 2010). The flow curve (η vs. 𝛾̇) was well fitted to the Sisko equation (Sisko, 1958): 𝜂=𝜂∞+𝐾𝛾̇𝑛−1 (Equation S1) where η∞, K and n are parameters accounting for the viscosity of the Newtonian flow unit, the consistency factor and the power law index, respectively (Blissett and Rowson, 2013).-- [Electrolytic conductivity] To evaluate the applicability of the VRV in monitoring clay viscosity, dispersions with well-defined densities (either 1.55 or 1.39 g·cm-3) and quantities of sodium silicate (< 0.7 wt.%) were examined.-- [Thixotropy assessment and the effect of density] A further assessment of thixotropy with the VRV is shown in Figure S5. Viscometer curves η vs. t were measured on four samples of a clay dispersion batch, each subjected to different initial rest periods. After the rest time, the dispersion was put in contact with the vibrational probe, and η value was monitored for a time of 30 min.-- Experimental; Chemical composition and mineralogical characterization; Flow curve; Electrolytic conductivity; Deflocculation curve; Thixotropy assessment and the effect of density., Peer reviewed

(A–D, G–J’) S9 egg chambers of the designated genotypes stained with anti-pSqh (red) and the DNA marker Hoechst (blue) showing pSqh levels in the NCs (A–D) and the BCs (G–J’). (E, F, K, L) Quantification of pSqh levels in the NCs (E, F) and in the BCs (K, L) of egg chambers of the specified genotypes. G’–J’ Magnifications of the white boxes in G–J. The statistical significance of differences was assessed with a t test, * P value < 0.05, ** P value < 0.01, and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bar in A, 20 μm. The raw data underlying panels E, F, K, and L are available in S1 Data., Peer reviewed

A–D) Schematic drawings of early S9 (A), middle S9 (B), late S9 (C), and S10 egg chamber illustrating the BCs (yellow), NCs (gray), FCs (purple), BM (green), and the point of ablation in the NCs (blue bar). (A’–D’) Images of life control egg chambers of the indicated developmental stages expressing Resille-GFP before and after NC bonds are ablated. Blue bars indicate points of ablation. (E) Schematic representation of an egg chamber and the point of ablation in NCs. (F, G) Quantification of the initial velocity of vertex displacement of the indicated ablated bonds. The statistical significance of differences was assessed with a t test, * P value < 0.05 and ** P value < 0.01. Horizontal and vertical lines indicate mean and SD, respectively. Scale bar in A’–D’, 10 μm. The raw data underlying panels F and G are available in S1 Data., Peer reviewed

Experimental and computational details, characterization data, rate constants measured, Cartesian coordinates and energies of the stationary points located, and crystallographic data for (±)-6 and (SP)-12., Peer reviewed

[Background] Diffuse large B cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma worldwide. DLBCL is an aggressive disease that can be cured with upfront standard chemoimmunotherapy schedules. However, in approximately 35-40% of the patients DLBCL relapses, and therefore, especially in this setting, the search for new prognostic and predictive biomarkers is an urgent need. Natural killer (NK) are effector cells characterized by playing an important role in antitumor immunity due to their cytotoxic capacity and a subset of circulating NK that express CD8 have a higher cytotoxic function. In this substudy of the R2-GDP-GOTEL trial, we have evaluated blood CD8+ NK cells as a predictor of treatment response and survival in relapsed/refractory (R/R) DLBCL patients., [Methods] 78 patients received the R2-GDP schedule in the phase II trial. Blood samples were analyzed by flow cytometry. Statistical analyses were carried out in order to identify the prognostic potential of CD8+ NKs at baseline in R/R DLBCL patients., [Results] Our results showed that the number of circulating CD8+ NKs in R/R DLBCL patients were lower than in healthy donors, and it did not change during and after treatment. Nevertheless, the level of blood CD8+ NKs at baseline was associated with complete responses in patients with R/R DLBCL. In addition, we also demonstrated that CD8+ NKs levels have potential prognostic value in terms of overall survival in R/R DLBCL patients., [Conclusion] CD8+ NKs represent a new biomarker with prediction and prognosis potential to be considered in the clinical management of patients with R/R DLBCL., [Clinical trial registration] https://www.clinicaltrialsregister.eu/ctr-search/search?query=2014-001620-29 EudraCT, ID:2014-001620-29., Peer reviewed

(A–B’) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. Scale bar in A’ and B’, 20 μm., Peer reviewed

TG/DTG/SDTA-MS and DSC curves for the series of compounds S1 and S2, emission and excitation spectra of yttrium-based compounds Za1 and Za2, section of synthesis and structural characterization for DZa7, and X-ray crystallographic information (CIF files) for the series of compounds S1 and S2, and compound DZa7., Peer reviewed

Materials and methods: Diagnosis of VSP: Vasospasm diagnosis was performed via clinical symptoms, transcranial Doppler, or angiography between days 0 to 14 after the bleeding event. Symptomatic vasospasm was defined as worsening of the level of consciousness of ≥2 points on the Glasgow Coma Scale, or by the appearance of a focal defect (such as hemiparesis, aphasia, apraxia, hemianopia, or neglect) between days 4 and 14 after the bleeding event, not attributable to rebleeding, hematoma, hydrocephalia, metabolic alterations, infection, or oversedation. Transcranial Doppler exploration (Doppler Multidop-P, DWL) was performed with a 2-MHz ultrasound probe through the temporal window, in both hemispheres. The first study was carried out on the third day, and every day between days 3 and 14 after aSAH, and beyond this date if the transcranial Doppler recording was not normal. Transcranial Doppler vasospasm or sonographic vasospasm was defined as the mean flow velocity (MV) in any vessel of anterior cerebral circulation ≥120 cm/s. If the MV obtained reached 120 cm/s, the Lindegaard index was calculated by simultaneously recording the homolateral extracranial internal carotid artery at the submandibular site and the MV of the middle cerebral artery. The MV middle cerebral artery/MV extracranial internal carotid artery ratio of >3 was classified as sonographic vasospasm. Patients with MV >120 cm/s and Lindegaard index <3 were diagnosed with increased cerebral blood flow. Using digital subtraction angiography, angiographic vasospasm was defined as a reduction in the diameter of the cerebral artery by more than two-thirds of its baseline caliber., Supplemental Table 1. Statistical analysis of the expression of surface adhesion molecules in circulating and adherent lymphocytes and monocytes. n= 20 control subjects and 21 patients. *, **, *** P≤ 0.05, 0.01, 0.001., Supplemental Figure 1. Gating strategy for the immunocharacterization of PBMCs. (A) Identification of the cells of interest performed by SSC-A and FSC-A. (B) Singlets filter by FSC-A and FSC-H. (C) Alive cells by exclusion of 7-AAD. (D) Identification of PBMCs by SSC-A and expression of CD45. (E) Identification of lymphocytes and monocytes by SSC-A and expression of CD11b. (F) Identification of lymphocyte subpopulations by expression of CD3 and CD19., Supplemental Figure 2. Expression of surface adhesion molecules in circulating and adhered T lymphocytes and monocytes (these data are the same that are shown on Figure 4 of the manuscript, but shown patient by patient). Only statistically significant changes in the adhesion molecules are presented. Comparisons between experiments from the same patients were assessed by paired t test (* P≤ 0.05 vs. control; #P≤ 0.05 vs. P24h)., Peer reviewed

(A, A’) S9 triple>LanB1RNAi (B) egg chambers stained with anti-LanB1 antibody (green), the DNA marker Hoechst (blue) and the PC marker Fasciclin III (FasIII, red). (B–C’) Stills taken from live imaging of BCs from control (C306Gal4; slboGal4; tslGal4) and C306; slbo; tsl>LanB1RNAi egg chambers. (D) Quantification of the migration defects in egg chambers of the indicated genotypes. The statistical significance of differences was assessed with a t test. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A” and B”, 20 μm. The raw data underlying panel D are available in S1 Data., Peer reviewed

(A) Schematic drawing of an early S9 egg chamber illustrating the BCs (yellow), NCs (gray), FCs (purple), BM (green), and the position where AFM measurements were taken (arrow). (B) Comparison of the apparent elastic modulus K in egg chambers of the designated genotypes. (C) Schematic drawing of a middle S9 egg chamber illustrating the BCs (yellow), NCs (gray), FCs (purple), BM (green), the mirror region (pink square) and the positions where AFM measurements were taken, anterior to the mirr region (A, black arrow) and in the mirr region (C, pink arrow). (D, E) Comparison of the apparent elastic modulus K in the mirr region (C) and anterior to the mirr region (E), in 4 control mirrGal4 (D) and mirr>EHBP1mCh egg chambers. A minimum of 4 different readings (circles)/egg chamber were taken. Horizontal lines in B, D, and E represent mean values. The statistical significance of differences was assessed with a t test, * P value < 0.05, ** P value < 0.01, and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. The raw data underlying panels B, D, and E are available in S1 Data., Peer reviewed