Resultados de investigación

6 pages. -- Table S1. Measured transition frequencies of 13C1 TFE isotopologue. -- Table S2. Measured transition frequencies of 13C2 TFE isotopologue. -- Table S3. Experimental Kraitchman’s coordinates (in Å) of g CF3CH2OH and PFPTg in their respective principal inertia axis systems. -- Table S4. The r0, rs and re of the C-C distance. The values of gauche conformer of ethanol are added for the comparison. -- Table S5. Measured transition frequencies of PFP-OD isotopologue., Peer reviewed

El presente trabajo ha sido financiado por Chiesi., Peer reviewed

The dataset includes additional data for the journal paper “Shock cooling of a red-supergiant supernova at redshift 3 in lensed images” by W. Chen et al., 2022. As described in the paper, these data include the MMT spectroscopic data, the HST coaddition and image differencing data, the GALFIT scripts and resulting models, the HST photometry of the SN host galaxy, the lens model, the SN light curve fitting script and resulting MCMC data, and plots of distributions of the model parameters and the best-fit light curves., Peer reviewed

Methods to induce analgesia and anesthesia have been used in 71 crustaceans for research pur-poses, handling, transport or stunning. A non-systematic literature search was carried out crus-tacean anesthetic methods. We prepared this on-line tool based on datasette (https://datasette.io/), a no-code open source solution for simple web-based database frontends that allows exploring and publishing data by method, analgesic/anesthetic, species, life stage or sex., as well as other data including environmental conditions (temperature, salinity, light), way of administration, dosage, and induction and recovery times. Use of this tool for selecting anesthetics in crustaceans is free but we ask that you please cite the following publication that explains the tool in any published outputs: Rotllant, G.; Llonch, P.; García del Arco, J.A.; Chic, Ò.; Flecknell, P.; Sneddon, L.U. Methods to Induce Analgesia and Anesthesia in Crustaceans: A Supportive Decision Tool. Biology 2023, 12, 387. https://doi.org/10.3390/biology12030387, With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), Peer reviewed

Table S1. Search strategy. Table S2. AMSTAR-2 Checklist., Peer reviewed

Figure S1. A) and B) TEM images of YP50F + 10wt% Bi2S3. C) and D) TEM images of YP50F + 10wt% Bi2S3 + MPA with 50 nm scale and 20 nm scale. Arrows have been included to indicate the location of Bi2S3 NCs. Figure S2. SEM images with of A) YP50F, B) YP50F + 5wt% Bi2S3 and C) YP50F + 10wt% Bi2S3 with secondary electrons (SE) mode and with back scattered diffraction mode (BSED) (D, E, F respectively) in which heavier elements appear brighter and in our case is related to the presence of Bi2S3 nanocrystals (scale is 5 µm). Magnification is x20000. Arrows have been included to indicate the location of Bi2S3 NCs. Figure S3. SEM images of A) YP50F + 5wt% Bi2S3 + MPA and B) YP50F + 10wt% Bi2S3 + MPA in SE and in BSED mode (C and D respectively). Arrows have been included to indicate the location of Bi2S3 NCs. Figure S4. SEM-EDS elemental mapping of (a) YP50F, (b) YP50F + 5wt% Bi2S3, (c) YP50F + 10wt% Bi2S3, and (d) as-synthesized Bi2S3 NCs. C in green, S in yellow and Bi in blue. Figure S5. FTIR spectra of YP50F (black), YP50F + 5wt% Bi2S3 (red), YP50F + 10wt% Bi2S3 (blue), YP50F + 5wt% Bi2S3 +MPA (green) and YP50F + 10wt% Bi2S3 + MPA (orange) after 250 scans, 4 cm-1 resolution, from 900 to 2000 cm-1. Figure S6. Nitrogen adsorption/desorption isotherm at 77K to determine the surface area. Figure S7 Electrodes operation voltammetry in YP50F + Bi2S3 in 1 M Li2SO4 at 5 mV·s-1. Cell voltage 1.5 V. Figure S8 Negative electrode operation voltammetry in YP50F + 10wt% Bi2S3 in 1 M Li2SO4 at 5 mV·s-1. Cell voltage 0.8V and 1.5 V. Figure S9 Metallic deposition of bismuth on glass fiber separator of the cell YP50F + Bi2S3 in 1 M Li2SO4 at 1.5 V. Figure S10 Scheme of the 3-mercaptopropionic acid (MPA) ligand exchange treatment where MPA molecules substitute the stabilizing oleic acid chains on the surface of the Bi2S3 nanocrystals. Most of MPA molecules bond to the NCs through the carboxylic acid end. Blue spheres represent carboxylic acid groups and green spheres, thiol groups. Figure S11 Comparison of the negative electrode responses in 5wt% Bi2S3 and 5wt% Bi2S3 + MPA. (cell polarized to 1.5 V) in 1 M Li2SO4 at 2.5 mV·s-1. Figure S12 Specific capacitances calculated from SPECS data of the negative electrode of YP50F (A), 5wt% Bi2S3 + MPA (B) and 5wt% Bi2S3 (C) in 1 mol L-1 NaI with 2:1 electrode mass ratio. Figure S13 Ragone plot for different negative-to-positive electrode mass ratios of the systems. Comparison with organic medium. Specific values expressed per mass of both electrodes. Figure S14 Bode plot (ac) and Ragone plot (dc) in wide discharge rate spectrum to analytically determine RC time constant. Figure S15 Cyclability of the system (1 A g-1) (-) YP50F + 10wt%Bi2S3 + MPA // YP50F (+) (1 mol L-1 NaI) m-:m+ = 2:1. Table S1 Elemental composition of pristine electrode containing 10wt% Bi2S3 and electrodes from the systems operating at different upper cell voltage limits 0.8 V and 1.5 V., Peer reviewed

Appendix 1 methods. Appendix 2 search and search results. Appendix 3_Effects of the interventions and certainty of evidence. Appendix 4 Forest plots. Appendix 5. Summary of Findings (SOF) tables., Peer reviewed

Details of X-ray crystallography data collection and structure solution, magnetometry, heat capacity data collection details, and additional figures., Peer reviewed

Appendix 1 methods. Appendix 2 search and search results. Appendix 3_Effects of the interventions and certainty of evidence. Appendix 4 Forest plots. Appendix 5. Summary of Findings (SOF) tables., Peer reviewed

9 pages. -- Figure S1. LC3 binding to liposomes is enhanced by membrane curvature and, in a dose-dependent manner, by CL. Interaction of LC3 proteins with vesicles of different radii and/or CL contents was measured by a vesicle flotation assay. -- Figure S2. Increasing ionic strength of the medium decreases the binding of LC3 to CL. Effect of the increased ionic strength on the interaction of LC3 proteins with CL-containing membranes, measured by a vesicle flotation assay. -- Figure S3. Changing a specific LC3C N-terminal residue has no effect on the protein binding to CL. -- Figure S4. Autophagy quantification with native GFP-LC3/GABARAP proteins and with non-conjugatable mutants confirms that most puncta in cells are autophagic vesicles and not aggregates. -- Figure S5. LC3A puncta and their colocalization with mitochondria increase with rotenone and CCCP treatment. Cells were co-transfected with DsRed2-Mito7 (DsRed) and with GFP-tagged WT or mutant LC3A. Vehicle (Veh) controls were treated with DMSO. -- Figure S6. The LC3B-AK double mutation that increases LC3B binding to CL in vitro does not have a comparable effect in cells. Cells were transfected with GFP-tagged WT or mutant LC3 protein. Mitochondria were labeled using MitoTracker Red, prior to the treatments. Vehicle (Veh) controls were treated with DMSO. -- Figure S7. mRNA and protein expression of LC3A, LC3B and LC3C in SH-SY5Y cells. -- Table S1. Primers used to perform site-directed mutagenesis. -- Table S2. Primers used to perform RT-qPCR. -- Table S3. siRNA synthesized by IDT to silence LC3A and LC3B proteins., Externalization of the phospholipid cardiolipin (CL) to the outer mitochondrial membrane has been proposed to act as a mitophagy trigger. CL would act as a signal for binding the LC3 macroautophagy/autophagy proteins. As yet, the behavior of the LC3-subfamily members has not been directly compared in a detailed way. In the present contribution, an analysis of LC3A, LC3B and LC3C interaction with CL-containing model membranes, and of their ability to translocate to mitochondria, is described. Binding of LC3A to CL was stronger than that of LC3B; both proteins showed a similar ability to colocalize with mitochondria upon induction of CL externalization in SH-SY5Y cells. Besides, the double silencing of LC3A and LC3B proteins was seen to decrease CCCP-induced mitophagy. Residues 14 and 18 located in the N-terminal region of LC3A were shown to be important for its recognition of damaged mitochondria during rotenone- or CCCP-induced mitophagy. Moreover, the in vitro results suggested a possible role of LC3A, but not of LC3B, in oxidized-CL recognition as a counterweight to excessive apoptosis activation. In the case of LC3C, even if this protein showed a stronger CL binding than LC3B or LC3A, the interaction was less specific, and colocalization of LC3C with mitochondria was not rotenone dependent. These results suggest that, at variance with LC3A, LC3C does not participate in cargo recognition during CL-mediated-mitophagy. The data support the notion that the various LC3-subfamily members might play different roles during autophagy initiation, identifying LC3A as a novel stakeholder in CL-mediated mitophagy., This work was supported in part by the Spanish Ministerio de Ciencia e Innovación (MCI), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) (grant No. PGC2018-099857-B-I00), by the Basque Government (grants No. IT1625-22 and IT1270-19), by Fundación Ramón Areces (CIVP20A6619), by Fundación Biofísica Bizkaia and by the Basque Excellence Research Centre (BERC) program of the Basque Government. MI and YV were recipients of predoctoral FPU fellowships from the Spanish Ministry of Science Innovation and Universities (FPU16/05873, FPU18/00799), UB thanks the University of the Basque Country for a predoctoral contract, JHH was supported by a Postdoctoral Fellowship from the Basque Government., Peer reviewed