BUSCADOR RECOLECTA

Peer reviewed

Peer reviewed

[Description of methods used for collection/generation of data: ] X-ray absorption spectroscopy and X-ray magnetic circular dichroism in photoemission electron microscopy., [Methods for processing the data] Intensity extracted from sequence of images at different photon energies, after manual drift correction. Vector magnetization extracted using the protocol described in https://surfmoss.iqf.csic.es/en/2013-04-02-10-34-02/manuals-howtos/90-vector-xcmd-peem-spleem, We present the in situ modification of the magnetocrystalline anisotropy of iron-rich cobalt-ferrite epitaxial islands by changing their composition. Crystalline cobalt-ferrite spinel islands have been grown by oxygen-assisted molecular beam epitaxy on a Ru(0001) single crystal. The micro-crystals are several micrometers wide with heights of tens of nanometers. Their spatially-resolved vector magnetization is mapped by x-ray magnetic circular dichroism in photoemission microscopy at the L3 absorption edges of Co and Fe. Thick islands present a closure-like magnetic domain structure with the magnetization directions predominantly aligned along the projections of the easy magnetic axes onto the (111) surface plane. Thinner islands are more affected by growth defects and present a more complex domain structure. Upon deposition of additional Fe in an oxygen background pressure, Co is found to segregate out of the spinel islands, while their Fe content increases. This produces a reduction in the magnetocrystalline anisotropy, which manifests as a reorientation of the magnetization directions towards the edges of the islands, reflecting an increased contribution of the shape anisotropy, AMADEUS PID2021-124585NB-C31, PID2021-124585NB-C32), Nanobond (TED2021-130196B-C22, TED2021-130957B-C54), PID2020-117024GB-C43, TED2021-130957B-C54., File List: - Characterization of as-grown sample - XAS PEEM Images: Fe, Co, O folders - XAS XMCD Images at 0, 60, and 120 degrees azimuthal rotation: Fe - Growth sequence of LEEM images - Characterization of modified sample - XAS PEEM Images: Fe, Co - XAS XMCD Images at 0, 60, 120 degrees azimuthal rotation: Fe, Co - Micromagnetic simulations - Input files - Auxiliary data - Calibration of counts vs. Channel plates voltage - Logbook of experiments, Peer reviewed

Dispersal is essential for all organisms to enable colonization of new areas. Like many other aquatic invertebrates, Anostraca (Crustacea) disperse passively as dormant fertilized eggs. Here, we first study the genetic diversity of Branchinecta fairy shrimps (identified morphologically as B. readingi) inhabiting lakes in Saskatchewan and British Columbia, Canada. Secondly, we attempt to reconstruct the phylogeny and dispersal events that explain their genetic distribution. Finally, we compare genetic diversity and distribution of Branchinecta sp. and Artemia franciscana, the two anostracans inhabiting the Saskatchewan lakes but with different salinity niches. To this aim, we sequenced CO1 and 16S gene fragments of the Branchinecta individuals collected from eight Saskatchewan lakes. We found a relatively high number of haplotypes for both markers (79 in CO1 and 76 in 16S) in Branchinecta from Saskatchewan. Three genetic clades at the CO1 gene region were found in Saskatchewan Branchinecta, with one of these clades being widely recorded previously in the USA (but described as B. mackini). In contrast, 54 CO1 haplotypes were previously recorded in A. franciscana in Saskatchewan for which all CO1 sequences belonged to one genetic clade. This suggests that Branchinecta sequences might be originating from several distinct Ice Age refugia, contrary to A. franciscana which might be originating from a single refuge. High density of suitable habitats (>100,000 lakes in Saskatchewan), weak priority effects and frequent dispersal events could explain high genetic diversity found in the studied Branchinecta. However, we found no genetic support for a distinction between B. readingi and B. mackini., Peer reviewed

FIGURE 1: XPS raw data of the (a) C1s, (b) Ti2p, (c) Zr3d and (d) O1s-V2p lines, respectively, after an annealing and in situ oxygen plasma treatment (50 W for 15 min) and annealing at four different temperatures: 100 °C, 130 °C, 150 °C and 200 °C. FIGURE 2: XPS raw data of the (a) C1s, (b) Ti2p, (c) Zr3d and (d) O1s-V2p lines, respectively, of an as-received sample, after an in situ argon (80%)/oxygen (20%) plasma treatment (60 W for 2h) and after an annealing at 150 °C overnight. FIGURE 3: XPS raw data of the O1s-V2p lines corresponding to different sequential plasma and annealing cycles performed at 150 °C overnight in all cases. FIGURE 4: Normalized O1s area ratio as a function of annealing at 100, 130, 150 and 200 °C, respectively. FIGURE 5: XPS raw spectra corresponding to the O1s core level of Ti-Zr-V films undergoing plasma and 150 °C annealing cycles: (a) 50 W oxygen plasma for 15 min and (b) 60 W argon/oxygen plasma for 2 h. The intensity of the spectra corresponding after annealing have been multiplied by a factor of two. FIGURE 6: XPS raw data of the (a) C1s, (b) Ti2p, (c) Zr3d and (d) O1s-V2p lines, respectively, of a sample exposed to argon (80%)/oxygen (20%) plasma (60 W for 2 h) after an annealing at 150 °C overnight and the same sample stored in UHV (≈ 1×10-9 mbar) for 5 days. Spectra have been normalized to their maxima. FIGURE 7: XPS raw data of the Si2p lines corresponding to as-received samples and after different plasma treatments: oxygen plasma (50 W, 15 min), argon/oxygen (50 W, 2 h) and (60 W, 2 h). FIGURE S2: Experimental and simulated (using SESSA software) survey XPS spectra. FIGURE S3: XPS raw data of the C1s core level of an as-received Ti-Zr-V film, after an exposure to a first 50W oxygen plasma cycle for 15 min, after an overnight annealing at 150 °C and after a subsequent second 50W oxygen plasma cycle for 15 min. FIGURE S4: XPS raw data of the C1s core level of an as-received sample with the corresponding least-squares fits using the CasaXPS software after a Shirley-type background subtraction using a combination of Gaussian (70%) and Lorentzian (30%) functions under the constraint of identical full width at half maximum (FWHM) for all components. FIGURE S5: XPS raw data of the N1s core level of an as-received sample, after exposure to an argon/oxygen plasma (80%-20%) at 60 W for 2 and after annealing overnight at 150 °C. FIGURE S6: XPS raw data of the (a) C1s, (b) Ti2p, (c) Zr3d and (d) O1s-V2p lines, respectively, after an annealing at 130 °C for 24 h, oxygen plasma treatment (50 W for 15 min), annealing al 200 °C for 1 h and exposure at a base pressure of ≈ 5 × 10-7 mbar for 16 h., [Description of methods used for collection/generation of data] Data acquired with a SPECS PHOIBOS150 hemispherical analyser with a monochromatic X-ray source (1486.6 eV) operated at 300W. The overall experimental resolution, including the contribution from the hemispherical analyser (energy and angle) and the monochromatic X-ray source, is better than 0.6 eV for a pass energy of 20 eV. The reported binding energies have been determined with an error of ±0.1 eV and are referred to the Fermi level of the analyser, which is periodically determined by measuring the photoelectron energies from an atomically clean reference Au(111) sample. No correction to the measured binding energies has been applied to the spectra., [Methods for processing the data] CasaXPS software version 2.3.16 PR 1.6., Original raw data of XPS spectra obtained in ORIGIN format. Columns (X) correspond to binding energies and columns Y to XPS intensity (Y). The different plasma and annealing conditions are indicated in the corresponding columns (“in Comments”). Labels in “Long name” files correspond to the original CasaXPS files names. Photon energy is 1486.6 eV., - Spanish Ministry of Science and Innovation (MCIN) under Contract Nos. PGC2018-095032-B-100 and PID2021-124568NB-I00. - The ICN2 is funded by the CERCA program/Generalitat de Catalunya. - The ICN2 is supported by the Severo Ochoa Centres of Excellence programme, Grant CEX2021-001214-S, funded by MCIN/AEI/10.13039.501100011033., With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001214-S)., ile List: figure_01.opju; figure_02.opju; figure_03.opju; figure_04.opju; figure_05.opju; figure_06.opju; figure_07.opju; figure_S2.opju, figure_S3.opju, figure_S4.opju, figure_S5.opju, figure_S6.xls, Peer reviewed

Interaction energies for all calculated systems, energy decomposition analysis complete results, and Cartesian coordinates of the model system bromobenzene···methyl formate., Peer reviewed

This database consists of three main parts: Table S1, which records extinct or extirpated vascular plant species from Macaronesia; Table S2, which summarizes biogeographic and demographic variables characterizing each Macaronesian island where vascular plant extinction events have occurred; and Tables S3 and S4, which detail the entire native vascular flora of the Azores and Canary Islands, including both extinct (well-supported records) and extant species, categorized by nine biological traits. Additionally, the database includes several supplementary sheets containing all cited bibliographic references for each case., The creation of this database was supported by the Spanish Ministry of Science and Innovation (MICINN) projects (ASTERALIEN-PID2019-110538GA-I00 and DecodAdapt-PID2023-147122NB-I00) the Fundación BBVA project (INVASION-PR19_ECO_0046), and the Ministerio para la Transición Ecológica y el Reto Demográfico (JUNIPERADAPT, 2941/2022). ROR was supported by the University Teacher Training (FPU) program (FPU21/02585) funded by the Ministry of Universities and JP by the MICINN through the Ramón y Cajal Program (RYC-2016-20506). JM-L was funded by a Juan de la Cierva-Formación Fellowship (MICIIN; reference FJC2020-046353-I). AV research was supported by the Fundación Cajacanarias/La Caixa project (2022CLISA29)., Extinct_species_biogeographical_variables_&_biological_traits_Macaronesian_flora.xlsx, Peer reviewed

[Description of methods used for collection/generation of data] - Sanger sequencing - Fluorescent Fragment Analysis, This dataset contains fragment analysis and sequencing files of a comprehensive study of the alternatively-spliced exon 7 of the breast cancer susceptibility gene ATM. Regulation of alternative splicing is a multifactorial process that includes a wide array of regulatory sequences (SRE) such as splicing enhancers and silencers. We have bioinformatically and functionally mapped two SRE-rich regions within ATM exon 7. Then, we tested 2 control splice-site and 48 regulatory variants in minigene mgATM_4-9 with exons 4 to 9 that we had previously reported (Bueno-Martinez et al, 2022; PMID 35716007). A large fraction of SRE-variants (71%; 34/48) impaired exon 7 recognition, supporting suitability of the combined in silico and minigene approach. Nineteen variants showed strong impacts, producing higher expression levels of △(E7)-transcripts and <30% of the minigene full-length transcript. Finally, eight variants were classified as likely pathogenic according to the ACMG/AMP-based guidelines for the ATM gene., EAV-S lab is supported by grants from the Spanish Ministry of Science and Innovation, Instituto de Salud Carlos III (PI20/00225; PI23/00047) co-funded by FEDER from Regional Development European Funds (European Union)., Folder: 1.Sequences - Sub-folder cDNA. Transcript Sequencing: 94 *.ab1 files of transcripts generated by microdeletions and variants introduced into minigene mgATM_4-9. - Sub-folder: Minigenes. Sequence files of wild type and mutant constructs: 51 *.ab1 files. • Folder: 2.Fragment_Analysis. Fluorescent Fragment Analysis of plicing assays: 159 *.fsa files of fluorescent fragment analysis of RT-PCRs of microdeletions and variants., Peer reviewed

The metabotropic glutamate receptors (mGlus) are class C G protein-coupled receptors (GPCR) that form obligate dimers activated by the major excitatory neurotransmitter L-glutamate. The architecture of mGlu receptor comprises an extracellular Venus-Fly Trap domain (VFT) connected to the transmembrane domain (7TM) through a Cysteine-Rich Domain (CRD). The binding of L-glutamate in the VFTs and subsequent conformational change results in the signal being transmitted to the 7TM inducing G protein binding and activation. The mGlu receptors signal transduction can be allosterically potentiated by positive allosteric modulators (PAMs) binding to the 7TMs, which are of therapeutic interest in various neurological disorders. Here, we report the cryoEM structures of metabotropic glutamate receptor 5 (mGlu5) purified with three chemically and pharmacologically distinct PAMs. We find that the PAMs modulate the receptor equilibrium through their different binding modes, revealing how their interactions in the 7TMs impact, Peer reviewed

Symptoms of illness offer insights into an organism’s condition, altering social signals that affect others' behavior. Nestling birds employ begging signals to solicit parental care, but the extent to which begging reflects nestling health beyond hunger remains controversial. We investigated how experimentally induced changes in health affect begging signals in spotless starling (Sturnus unicolor) nestlings. To alter health status, we administered an immune challenge by injecting lipopolysaccharide (LPS) or a control substance (PBS) and observed begging behavior under controlled food deprivation conditions. LPS-treated nestlings exhibited delayed gaping, reduced stretching, and less begging time compared to control nestlings. Moreover, LPS-treated nestlings produced calls with higher spectral entropy, particularly when deprived of food for longer. Our findings indicate that begging signals are sensitive to induced sickness. However, addressing mechanistic issues is crucial for effectively testing whether begging reflects nestling conditions as a signal of quality in future experimental setups., Peer reviewed