BUSCADOR RECOLECTA

Quantification of the gene expression in a strain collection of Escherichia coli containing AIEC (Adherent Invasive Escherichia coli) and non-AIEC (non-Adherent Invasive Escherichia coli) strains by RT-qPCR. Each file contains the quantification of different genes included in this study, corresponding to the four Fluidigm chips used to quantify gene expression:

"Fluidigm1": Quantification of the genes gapA, 0163, 0408, 0410, 0413, 0414, 0415, 0420, 0422, 0577, 0938, 1003, 1063, 1154, 1184, 1196, 1688, 1689, 1690, 1728, 1971, 2009, 2033, 2505, 2523, 2896, 2902, 2903, 2904, 3227

"Fluidigm2": Quantification of the genes chiA, fimH, 0036, 0512, 0794, 1058, 1255, 1256, 1257, 1395, 1396, 1397, 1815, 2831, 2857, 3046, 3163, 0354, 0418, 0511, 0912, 1071, 1198, 1838, 1935, 1983, 2730, 3029

"Fluidigm3": Quantification of the genes aidA, adiC, gapA, potE, 0410b, 0410c, 0410d, 0410e, 0410f, 0601, 3029a, 3029b, 3046a, 3046b, 3172a, 3172b in the SN fraction

"Fluidigm4": Quantification of the genesaidA, adiC, gapA, potE, 0410b, 0410c, 0410d, 0410e, 0410f, 0601, 3029a, 3029b, 3046a, 3046b, 3172a, 3172b in the A/I fraction

Dataset of comparative transcriptomics between Adherent Invasive Escherichia coli (AIEC)/non-Adherent Invasive Escherichia coli (non-AIEC) strain pairs.

The dataset contains six files: four of them contain the comparisons made between pairs of AIEC and non-AIEC strains. In addition, there are two additional files comparing comparisons made with the same pairs of strains but different conditions.

In these files, you can find the gene expression values in each sample analyzed. Moreover, the log2 fold change, p-value, q-value and test_stat are displayed for each comparison. The four comparisons performed are:

- Comparison 1 corresponds to comparative transcriptomics between AIEC17 vs ECG28 strains of the supernatant fraction of the infected cell culture.

- Comparison 2 corresponds to comparative transcriptomics between AIEC17 vs ECG28 strains of the eukaryotic fraction, containing bacteria adhered and invading intestinal epithelial cells.

- Comparison 5 corresponds to comparative transcriptomics between AIEC07 vs ECG04 strains of the supernatant fraction of the infected cell culture.

- Comparison 6 corresponds to comparative transcriptomics between AIEC07 vs ECG04 strains of the eukaryotic fraction, containing bacteria adhered and invading intestinal epithelial cells.

In each file, statistically significant differences of gene expression are selected and displayed in another sheet.