Resultados de investigación

Supplementary Material 7: Fig. 7. A. Expression levels of total and active EGFR pancreatic cancer cell lines. One milligram of total protein from each cell line was immunoprecipitated with an anti-EGFR antibody to assess both the total amount and the phosphorylation status of EGFR. Calnexin was used as a loading control. B. Relationship between TGFA and EGFR expression in pancreatic cancer. Data was obtained from the TNMplot online tool., Predoctoral contract Cancer Research Foundation of the Salamanca University (FICUS) Instituto de Salud Carlos III through a Miguel Servet program Ministry of Economy and Competitiveness of Spain Instituto de Salud Carlos III through CIBERONC Junta de Castilla y León ALMOM ACMUMA UCCTA CRIS Cancer Foundation and the Regional Development Funding Program (FEDER) “A way to make Europe”, Peer reviewed

Predoctoral contract Cancer Research Foundation of the Salamanca University (FICUS) Instituto de Salud Carlos III through a Miguel Servet program Ministry of Economy and Competitiveness of Spain Instituto de Salud Carlos III through CIBERONC Junta de Castilla y León ALMOM ACMUMA UCCTA CRIS Cancer Foundation and the Regional Development Funding Program (FEDER) “A way to make Europe”, Peer reviewed

Peer reviewed

Understanding the trophic ecology of herbivorous and detritivorous fishes is essential for evaluating their ecological roles in coral reef ecosystems. In this study, we combined bulk stable isotope (δ15N and δ13C) and fatty acid analyses to investigate trophic partitioning and dietary resource use among herbivorous and detritivorous fishes from the Great Barrier Reef, Australia. Isotopic niches and fatty acid profiles confirmed significant trophic partitioning among algivores, detritivorous surgeonfishes, and parrotfishes. We also applied mixing models based on these ecological tracers to quantify the contributions of basal dietary sources to the fish. Our findings further support previous dietary knowledge for several species, including algivorous acanthurids, kyphosid chubs, and the rabbitfish Siganus doliatus. However, they also highlight trophic niche specializations within these groups, particularly in Naso unicornis, which assimilates substantial dietary protein from epiphytic cyanobacteria despite a macroalgal diet, and in the detritivorous Ctenochaetus striatus, which exhibited isotopic similarities to parrotfishes but differed in fatty acid composition, likely due to a higher intake of diatoms. Additionally, our analyses reinforce the distinctive dietary composition of parrotfishes, emphasizing the complexity of their feeding biology, in which microscopic photoautotrophs such as cyanobacteria and dinoflagellates play a key dietary role that has often been overlooked in previous studies on their nutritional ecology. Furthermore, these findings underscore the usefulness of multi-tracer approaches in refining our understanding of coral reef fish trophic ecology., Peer reviewed

[References:] Byrne, Maria (1990): Annual reproductive cycles of the commercial sea urchin Paracentrotus lividus from an exposed intertidal and a sheltered subtidal habitat on the west coast of Ireland. Marine Biology, 104(2), 275-289, https://doi.org/10.1007/BF01313269 Lozano, J; Galera, J; López,, S; Turon, X; Palacin, C (1995): Biological cycles and recruitment of Paracentrotus lividus (Echinodermata: Echinoidea) in two contrasting habitats. Marine Ecology Progress Series, 122, 179-191, [Coverage:] Median Latitude: 27.980300 * Median Longitude: -15.656640 * South-bound Latitude: 27.760200 * West-bound Longitude: -15.829200 * North-bound Latitude: 28.150400 * East-bound Longitude: -15.414600, Biometric and reproductive data of three sea urchin common species, Paracentrotus lividus, Arbacia lixula, and Sphaerechinus granularis, in the Central-Eastern Atlantic's rocky shores were collected over a one-year sampling period, from June 2020 to May 2021, in different localities of Gran Canaria Island. The datasets include biometric measurements such as test diameter, height, total wet weight, gut weight, and gonad weight, along with reproductive data including sex and maturity stage for each individual sampled at the different localities. In addition, oocytes from mature females of each species were measured to compare the size between species. The collected specimens were identified in the laboratory to the lowest possible taxonomic level, and those selected for sex and sexual maturity analysis were examined following a methodology based on macroscopic observation of the gonads and histological analysis (Byrne, 1990; Lozano et al., 1995). Biometric measurements, such as test diameter, height, total wet weight, gut weight, and gonad weight, along with reproductive data, including sex and maturity stage for each individual sampled at the different localities, were collected. The collected specimens were identified in the laboratory to the lowest possible taxonomic level, and those selected for sex and sexual maturity analysis were examined following a methodology based on macroscopic observation of the gonads and histological analysis (Lozano et al., 1995)., Xunta de Galicia, grant/award no. IN606B-2024/018: Interacciones tróficas y procesos ecosistémicos en peces mesopelágicos (TROF-MP), Peer reviewed

[References:] Byrne, Maria (1990): Annual reproductive cycles of the commercial sea urchin Paracentrotus lividus from an exposed intertidal and a sheltered subtidal habitat on the west coast of Ireland. Marine Biology, 104(2), 275-289, https://doi.org/10.1007/BF01313269 Lozano, J; Galera, J; López,, S; Turon, X; Palacin, C (1995): Biological cycles and recruitment of Paracentrotus lividus (Echinodermata: Echinoidea) in two contrasting habitats. Marine Ecology Progress Series, 122, 179-191 Rueden, Curtis T; Schindelin, Johannes; Hiner, Mark C; DeZonia, Barry E; Walter, Alison E; Arena, Ellen T; Eliceiri, Kevin W (2017): ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics, 18(1), 529, https://doi.org/10.1186/s12859-017-1934-z, [Coverage:] Median Latitude: 27.980300 * Median Longitude: -15.656640 * South-bound Latitude: 27.760200 * West-bound Longitude: -15.829200 * North-bound Latitude: 28.150400 * East-bound Longitude: -15.414600, Biometric and reproductive data of three sea urchin common species, Paracentrotus lividus, Arbacia lixula, and Sphaerechinus granularis, in the Central-Eastern Atlantic's rocky shores were collected over a one-year sampling period, from June 2020 to May 2021, in different localities of Gran Canaria Island. The datasets include biometric measurements such as test diameter, height, total wet weight, gut weight, and gonad weight, along with reproductive data including sex and maturity stage for each individual sampled at the different localities. In addition, oocytes from mature females of each species were measured to compare the size between species. The collected specimens were identified in the laboratory to the lowest possible taxonomic level, and those selected for sex and sexual maturity analysis were examined following a methodology based on macroscopic observation of the gonads and histological analysis (Byrne, 1990; Lozano et al., 1995). After histological analysis, the ovaries of mature females of three sea urchin common species, Paracentrotus lividus, Arbacia lixula, and Sphaerechinus granularis, on the Central-Eastern Atlantic's rocky shores were photographed. The oocytes were measured using ImageJ software (Rueden 2017) to compare the sizes of these cells between species. The dataset includes the diameter of each oocyte measured and the code of each individual., Xunta de Galicia, grant/award no. IN606B-2024/018: Interacciones tróficas y procesos ecosistémicos en peces mesopelágicos (TROF-MP), Peer reviewed

[Related to:] Bode, Antonio; Álvarez, Marta; Ruiz-Villarreal, Manuel; Varela, Marta M (2019): Changes in phytoplankton production and upwelling intensity off A Coruña (NW Spain) for the last 28 years. Ocean Dynamics, 69(7), 861-873, https://doi.org/10.1007/s10236-019-01278-y Bode, Antonio; Alvarez-Ossorio, María Teresa; Cabanas, J; Miranda, Ana; Varela, M (2009): Recent trends in plankton and upwelling intensity off Galicia (NW Spain). Progress in Oceanography, 83(1-4), 342-350, https://doi.org/10.1016/j.pocean.2009.07.025 Bode, Antonio; Alvarez-Ossorio, María Teresa; Miranda, Ana; Ruiz-Villarreal, Manuel (2013): Shifts between gelatinous and crustacean plankton in a coastal upwelling region. ICES Journal of Marine Science, 1-9, https://doi.org/10.1093/icesjms/fss193 Bode, Antonio; Estévez, M Graciela; Varela, Manuel; Vilar, José A (2015): Annual trend patterns of phytoplankton species abundance belie homogeneous taxonomical group responses to climate in the NE Atlantic upwelling. Marine Environmental Research, 110, 81-91, https://doi.org/10.1016/j.marenvres.2015.07.017, [Project(s):] Adaptação Costeira às alterações climáticas: conhecer os riscos e aumentar a resiliência (MarRisk) seRies temporAles De oceanografIA en eL norte de ESpaña (RADIALES), [Coverage:] Latitude: 43.421660 * Longitude: -8.436660, Monthly series of hydrographic (temperature, salinity) and biogeochemical variables (nutrients, dissolved oxygen, particulate organic matter, chlorophyll, primary production), and zooplankton taxa abundance and biomass (dry weight) collected at one shelf station for various periods between 1990 and 2018 at A Coruña (NW Spain). These series are part of the long-term observational project RADIALES (Instituto Español de Oceanografía, IEO, Spain)., Peer reviewed

Tipo de dataset: Bases de datos, Bruxism is a repetitive activity in the masticatory muscles characterised by clenching or grinding of the teeth and/or stiffening or thrusting of the mandible, associated with dysregulation of autonomic nervous system activity and with changes in muscle tone and pressure pain threshold. Visceral manual therapy (VMT) has shown effects on vagal tone and autonomic nervous system modulation; however, its impact on patients with bruxism has not been studied. This study evaluated the effects of VMT on the autonomic nervous system in individuals with bruxism and whether these effects are accompanied by changes in muscle tone and pressure pain threshold of the masticatory muscles., [Methods:] A single-blind randomized clinical trial was conducted with 24 subjects diagnosed with “probable bruxism.” Participants were divided into an experimental group, which received VMT applied to the epigastric region, and a control group, which received a placebo technique. Variables measured included heart rate variability (HRV), normal-to-normal interval (SDNN), root mean square differences of successive normal-to-normal intervals (RMSSD), myotonometry of the masseter muscles and pressure pain threshold (PPT) measured at C4, masseter, and temporalis muscles. A repeated-measures ANOVA was used to analyze the effects of the intervention., [Results:] A significant time × group interaction was observed in HRV-SDNN (p = 0.04, η² = 0.12), suggesting a positive effect of VMT on autonomic regulation. Although HRV-RMSSD did not show long-term changes, significant variations were observed during the first session. No relevant changes were found in myotonometry, while PPT results showed hypoalgesic effects at C4 in the second measurement (p = 0.049, η² = 0.16) and at the left temporalis muscle in the first measurement (p = 0.01, η² = 0.07)., [Conclusion:] VMT appears to modulate vagal tone and improve sympathovagal balance in patients with bruxism, although no significant changes were observed in muscle tone or in most PPT variables. However, the localized hypoalgesic response at C4 and the left temporalis muscle suggests a potential pain-modulating effect. Further studies are needed to clarify the clinical relevance of these effects and their potential application in the management of bruxism., Peer reviewed

The dataset included 1 compressed folder: “2006_Habitat_Saladas_Alcañiz-Calanda.zip”. The folder comprises one GIS file generated in ArcGIS v.10.8 (“2006_Habitats_Saladas_Alcañiz-Calanda”). The shape is based on the information generated in the 2006 fieldwork and on the CORINE legend of the List of Habitats of Aragon (LHA) (Benito Alonso, 2011). Also, we included the natural habitat types of community interest (HIC) listed in Annex I of the Habitats Directive (92/43/EEC). The explanation of the attribute table is included in the document ‘Explanation.docx’ with the translation of the habitats described in Spanish and English of LHA and HIC. This shape presents two legend layers according to the presence of LHA (“2006_Habitats_Saladas_Alcañiz-Calanda_LHA.lyr”) and HIC (“2006_Habitats_Saladas_Alcañiz-Calanda_HIC.lyr”) in the majority habitat of each polygon. The abovementioned geographic layers are map projected in the European Datum ETRS89/UTM zone 30N., We presented the information of mapping habitat generated in the 2006 fieldwork. The studied wetland is the Saladas of Alcañiz-Calanda, catalogued in the Natura 2000 European Network, under the European Directives Birds (2009/147/EC) and Habitats (92/43/EEC)., The data presented here are a result of the research projects ISABEL, funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” and Instituto de Estudios Turolenses (IET) through the call for I+D+I Projects - EIT 2025., Peer reviewed

Time-Lapse Fluorescence Microphotography Data: This dataset contains raw files in .lif format from time-lapse fluorescence microphotography experiments, as detailed in Sanchez et al. (2024). Each dataset includes multiple images, and to facilitate interpretation, an additional Excel file titled "Overview of Time-Lapse Fluorescence Microphotography Datasets" is provided. This file includes key information such as plant genotypes and treatment of the plants in each image/position. In most experiments, a control plant (35S:D14-GFP; Col-0) was used for quality control purposes but was excluded from the data analysis. LUCIFERASE activity assays: This dataset contains raw .txt files from LUCIFERASE activity assays as described in Sanchez et al. (2024). The files are organized by figure and include the counts per second (cps) values as measured by the luminometer for each experiment. In addition, each file also provides a brief description including the plant genotypes, treatment details, and the time of treatment application. Phenotypic analysis of adult plants: A .txt file containing measurements of plant height (cm) and the number of branches (RI/RL) for plants analyzed in Sanchez et al. (2024)., El dataset se puede consultar y descargar en https://doi.org/10.5281/zenodo.13830644, Peer reviewed