Resultados de investigación

Los resultados de la encuesta se comentarán en el artículo "Spracherwerbsmotivation unter den spanischen Emigranten der neuen Generation" que se publicará próximamente., Encuesta perteneciente a un estudio que se realizó en la Universidad de Granada, en el marco de un proyecto de investigación sobre "crisis, emigración y aprendizaje del alemán", que tenía como fin presentar propuestas de mejora. La encuesta de 29 preguntas se dirijió a todos los jóvenes españoles en situación de emigración relativamente reciente, que viven, trabajan o buscan trabajo en un país de habla alemana. La encuesta se realizó de julio a noviembre de 2013. Se recopilaron las respuestas de 107 informantes., Survey belonging to a study that was conducted at the University of Granada, within the framework of a research project on "crisis, migration and learning German", whose aim was to present proposals to improve the situation. The survey consists of 29 questions and addresses all young Spaniards in situations of relatively recent immigration who are living, working or looking for work in a German-speaking country. The survey was conducted from July to November 2013. Responses of 107 respondents were collected.

[Organism] Rattus norvegicus, [Experiment type] Expression profiling by array, [Overall design] Breast tumors were induced with a single oral dosage of 7,12-dimethylbenz(alpha)anthracene (100 mg/kg body weight) in female Sprague-Dawley rats and subsequently treated with adriamycin (1 mg/kg body weight/week for 6 weeks) intravenously through lateral tail vein. Gene expression analysis was performed in paired samples as follows: ADR final trucut tumor vs initial trucut tumor (ADR final vs basal). For this assay, 5 samples were chosen according to histopathologic criteria (Bloom-Richardson grade II). Gene expression profiling was carried out using Affymetrix’s GeneChip technology, using the Rat Genome 230 2.0 array from this provider. All the protocols and apparatus were recommended by Affymetrix. Total RNA from frozen mammary tumors was extracted by RNeasy Mini kit and homogenized by QIAshredder columns according to manufacturer’s instructions. The quality and quantity of the obtained RNA was checked out through agarose electrophoresis and later spectrophotometry at 260/280 nm. Biotinylated cRNA was synthesized following the IVT labeling kit from Affymetrix and purified by the GeneChip Sample Cleanup Module from Affymetrix. The quality and quantity of the obtained cRNA was again checked out through agarose electrophoresis and posterior spectrophotometry at 260/280 nm. After hybridization, slides were washed and scanned following the manufacturer’s standard protocol. Intensity values were normalized by Robust Multichip Average method and subsequently these were filtered to remove the control sequences and those with a hybridization signal close to background. The spike controls were: BioB, BioC, BioD and Cre; because BioB was the least abundant in the samples, it was used to estimate the sensitivity of the experiment. The housekeeping control was GAPDH. After non-supervised clustering using Pearson correlation coefficient, statistical significance of gene expression was estimated by Student’s T test for paired samples, using GeneSpring GX 7.3 software (Agilent)., 1. GSM399913 Breast tumor biopsy_adriamycin_07SE125. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with adriamycin. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 09I1 (basal), 2. GSM399914 Total breast tumor_ adriamycin _07SE140. [Characteristics strain: ]Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: adriamycin (1 mg/kg weight/week, for 6 weeks) through the lateral tail vein after trucut biopsy. sacrifice: 7th week post-biopsy sample: 09I1 (final), 3. GSM399915 Breast tumor biopsy_adriamycin_07SE126. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with adriamycin. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 11D2 (basal), 4. GSM399916 Total breast tumor_ adriamycin _07SE141. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: adriamycin (1 mg/kg weight/week, for 6 weeks) through the lateral tail vein after trucut biopsy. sacrifice: 7th week post-biopsy sample: 11D2 (final), 5. GSM399949 Breast tumor biopsy_adriamycin_07SE127. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with adriamycin. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 12I1 (basal), 6. GSM399959 Total breast tumor_ adriamycin _07SE142. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: adriamycin (1 mg/kg weight/week, for 6 weeks) through the lateral tail vein after trucut biopsy. sacrifice: 7th week post-biopsy sample: 12I1 (final), 7. GSM399960 Breast tumor biopsy_adriamycin_07SE128. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with adriamycin. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 29I1 (basal), 8. GSM399961 Total breast tumor_ adriamycin _07SE143. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: adriamycin (1 mg/kg weight/week, for 6 weeks) through the lateral tail vein after trucut biopsy. sacrifice: 7th week post-biopsy sample: 29I1 (final), 9. GSM399980 Breast tumor biopsy_adriamycin_07SE129. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with adriamycin. Such biopsy was taken when tumor had 2cm3 volume. biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 43D1 (basal), 10. GSM399981 Total breast tumor_ adriamycin _07SE144. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: adriamycin (1 mg/kg weight/week, for 6 weeks) through the lateral tail vein after trucut biopsy. sacrifice: 7th week post-biopsy sample: 43D1 (final), The aim of this study was to compare the gene expression profile changes of DMBA-induced rat breast tumors after treatment with adriamycin. To this end, a cDNA microarray was performed (Affymetrix’s Rat Genome 230 2.0 array). This gene expression study was carried out on the tumor biopsy samples prior to adriamycin treatment, and compared with matched tumor biopsy samples after completion of the adriamycin treatment schedule.

Excel file with a list of 116 spanish journalists Twitter accounts. All of them are director of mass media (newspaper, radio, TV, et.al). We include: director name, media name, account name, numbers of tweets, number of followings, number of followers, ratio, et.al. This data is both for directors and media accounts. Extracted from Twitter on June 2013.

Recently, several sets of standardized food pictures have been created, supplying both food images and their subjective evaluations. However, to date only the OLAF (Open Library of Affective Foods), a set of food images and ratings we developed in adolescents, has the specific purpose of studying emotions toward food. Moreover, some researchers have argued that food evaluations are not valid across individuals and groups, unless feelings toward food cues are compared with feelings toward intense experiences unrelated to food, that serve as benchmarks. Therefore the OLAF presented here, comprising a set of original food images and a group of standardized highly emotional pictures, is intended to provide valid between-group judgments in adults. Emotional images (erotica, mutilations, and neutrals from the International Affective Picture System/IAPS) additionally ensure that the affective ratings are consistent with emotion research. The OLAF depicts high-calorie sweet and savory foods and low-calorie fruits and vegetables, portraying foods within natural scenes matching the IAPS features. An adult sample evaluated both food and affective pictures in terms of pleasure, arousal, dominance, and food craving, following standardized affective rating procedures. The affective ratings for the emotional pictures corroborated previous findings, thus confirming the reliability of evaluations for the food images. Among the OLAF images, high-calorie sweet and savory foods elicited the greatest pleasure, although they elicited, as expected, less arousal than erotica. The observed patterns were consistent with research on emotions and confirmed the reliability of OLAF evaluations. The OLAF and affective pictures constitute a sound methodology to investigate emotions toward food within a wider motivational framework., Hum-388 Research Group/Grupo de Investigación "Psicofisiología Humana y Salud"

In this dataset we show the total account of news for each university of Andalusia in Google News from 2011.

Datos complementarios del artículo: “Acquisition of Uropygial Gland Microbiome by Hoopoe Nestlings” publicado en Microbial Ecology (ISSN:0095-3628). DOI: https://doi.org/10.1007/s00248-017-1125-5

[Organism] Rattus norvegicus, [Experiment type] Expression profiling by array, [Overall design] Breast tumors were induced with a single oral dose of 7,12-dimethylbenz(alpha)anthracene (100 mg/kg body weight) in female Sprague-Dawley rats to test the antitumor power of orally administrated hydroxytyrosol (0.5 mg/kg body weight 5 days/week for 6 weeks). Gene expression analysis was performed in paired samples as follows: HT final trucut tumor vs initial trucut tumor (HT final vs basal). For this assay, 5 samples were chosen according to histopathologic criteria (Bloom-Richardson grade II). Gene expression profiling was carried out using Affymetrix’s GeneChip technology, using the Rat Genome 230 v2.0 array from this provider. All the protocols and apparatus were recommended by Affymetrix. Total RNA from frozen mammary tumors was extracted by RNeasy Mini kit and homogenized by QIAshredder columns according to manufacturer’s instructions. The quality and quantity of the obtained RNA was checked out through agarose electrophoresis and later spectrophotometry at 260/280 nm. Biotinylated cRNA was synthesized following the IVT labeling kit from Affymetrix and purified by the GeneChip Sample Cleanup Module from Affymetrix. The quality and quantity of the obtained cRNA was again checked out through agarose electrophoresis and posterior spectrophotometry at 260/280 nm. After hybridization, slides were washed and scanned following the manufacturer’s standard protocol. Intensity values were normalized by Robust Multichip Average method and subsequently these were filtered to remove the control sequences and those with a hybridization signal close to background. The spike controls were: BioB, BioC, BioD and Cre; because BioB was the least abundant in the samples, it was used to estimate the sensitivity of the experiment. The housekeeping control was GAPDH. After non-supervised clustering using Pearson correlation coefficient, statistical significance of gene expression was estimated by Student’s T test for paired samples using GeneSpring GX 7.3 software (Agilent)., [Platforms (1)] GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array, [Relations] BioProject PRJNA116997, 1. GSM399469 Breast tumor biopsy_hydroxytyrosol_07SE120. [Source name] Breast tumor biopsy, hydroxytyrosol, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with hydroxytyrosol. Such biopsy was taken when tumor had 2cm3 volume biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 66D2 (basal), 2. GSM399474 Total breast tumor_hydroxytyrosol_07SE135. [Source name] Total breast tumor, hydroxytyrosol, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: hydroxytyrosol (0,5 mg/kg weight/day, 5 days/week, for 6 weeks) by gavage after trucut biopsy. sacrifice: 7th week post-biopsy sample: 66D2 (final), 3. GSM399475 Breast tumor biopsy_hydroxytyrosol_07SE121. [Source name] Breast tumor biopsy, hydroxytyrosol, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with hydroxytyrosol. Such biopsy was taken when tumor had 2cm3 volume biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 67D3(basal), 4. GSM399476 Total breast tumor_hydroxytyrosol_07SE136. [Source name] Total breast tumor, hydroxytyrosol, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: hydroxytyrosol (0,5 mg/kg weight/day, 5 days/week, for 6 weeks) by gavage after trucut biopsy. sacrifice: 7th week post-biopsy sample: 67D3 (final), 5. GSM399477 Breast tumor biopsy_hydroxytyrosol_07SE122. [Source name] Breast tumor biopsy, hydroxytyrosol, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with hydroxytyrosol. Such biopsy was taken when tumor had 2cm3 volume biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 45D3 (basal), 6. GSM399478 Total breast tumor_hydroxytyrosol_07SE137. [Source name] Total breast tumor, hydroxytyrosol, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: hydroxytyrosol (0,5 mg/kg weight/day, 5 days/week, for 6 weeks) by gavage after trucut biopsy. sacrifice: 7th week post-biopsy sample: 45D3 (final), 7. GSM399892 Breast tumor biopsy_hydroxytyrosol_07SE123. [Source name] Breast tumor biopsy, hydroxytyrosol, basal moment. [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with hydroxytyrosol. Such biopsy was taken when tumor had 2cm3 volume biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 49I1 (basal), 8. GSM399893 Total breast tumor_hydroxytyrosol_07SE138. [Source name] Total breast tumor, hydroxytyrosol, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: hydroxytyrosol (0,5 mg/kg weight/day, 5 days/week, for 6 weeks) by gavage after trucut biopsy. sacrifice: 7th week post-biopsy sample: 49I1 (final), 9. GSM399894 Breast tumor biopsy_hydroxytyrosol_07SE124. [Source name] Breast tumor biopsy, hydroxytyrosol, basal moment . [Characteristics] strain: Sprague-Dawley gender: female age: 12 weeks tissue: Breast tumor. Trucut Biopsy of breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene before treatment with hydroxytyrosol. Such biopsy was taken when tumor had 2cm3 volume biopsy dimensions: 0.7 cm x 2.5 mm treatment: none sample: 51I1 (basal), 10. GSM399912 Total breast tumor_hydroxytyrosol_07SE139. [Source name] Total breast tumor, hydroxytyrosol, final moment. [Characteristics] strain: Sprague-Dawley gender: female age: 19 weeks tissue: Breast tumor. Breast tumor chemically induced with 7,12-dimethylbenz(a)anthracene at the moment of sacrifice. treatment: hydroxytyrosol (0,5 mg/kg weight/day, 5 days/week, for 6 weeks) by gavage after trucut biopsy. sacrifice: 7th week post-biopsy sample: 51I1 (final), The aim of this study was to compare the gene expression profile changes of DMBA-induced rat breast tumors after treatment with hydroxytyrosol (a natural compound from virgin olive oil). To this end, a cDNA microarray experiment was performed (Affymetrix’s Rat Genome 230 2.0 array). This gene expression study was carried out on the tumor biopsy samples prior to hydroxytyrosol treatment, and compared with matched tumor biopsy samples after completion of the hydroxytyrosol treatment schedule. The result of this study was the identification of several genes related to apoptosis, cell cycle arrest, proliferation, differentiation, survival and transformation-related genes.

Open data and code for main analyses in "A test-retest assessment of the effects of mental load on ratings of affect, arousal and perceived exertion during submaximal cycling".

El documento sólo puede leerse con ayuda del programa "Atlas.ti", Documentos en formato "Atlas.ti" que contienen los corpus y el análisis correspondientes a las reseñas españolas y alemanas. Son los datos brutos correspondientes a la ponencia "Análisis cualitativo de textos con un programa CAQDAS", presentado en el coloquio Hermeneus, en Soria, 2014. Es muy probable que dicha ponencia se publique en un futuro próximo.

[Organism] Homo sapiens, [Experiment type] Expression profiling by array, [Overall design] Adipose tissue and blood samples were obtained from 27 children, 14 obese (BMI adjusted for age and sex z score > 2) and 13 non obese undergoing appendix surgery. About 400 mg of adipose tissue was taken and immediately immersed in RNAlater solution and stored at -80°C for gene expression analysis. Informed consent was obtained from all patients after the nature of the study was explained, and the experimental design was approved, from an ethical and scientific standpoint, by the Hospital’s Ethical Committee responsible for research., [Platforms (1)] GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array, [Relations] BioProject PRJNA103477, 1. GSM243215 Adipose tissue_423_control_rep1. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.45, 2. GSM243216 Adipose tissue_425_control_rep2. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: 0.49, 3. GSM243217 Adipose tissue_426_control_rep3. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: F age: 8 year z score bmi: 0.12, 4. GSM243218 Adipose tissue_427_control_rep4. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 10 year z score bmi: -1.81, 5. GSM243219 Adipose tissue_428_control_rep5. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.64, 6. GSM243220 Adipose tissue_429_control_rep6. [Source name] Omental adipose tissue, normal weight, child. [Characteristics] tissue: omental adipose group: normal weight gender: M age: 7 year z score bmi: -0.36, 7. GSM243225 Adipose tissue_418_obese_rep1. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 11 years z score bmi: 2, 8. GSM243226 Adipose tissue_419_obese_rep2. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25, 9. GSM243227 Adipose tissue_420_obese_rep3. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 12 years z score bmi: 2.36, 10. GSM243228 Adipose tissue_421_obese_rep4. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 9 years z score bmi: 4.25, 11. GSM243275 Adipose tissue_422_obese_rep5. [Source name] Omental adipose tissue, obese, child. [Characteristics] tissue: omental adipose group: obese gender: M age: 10 years z score bmi: 2.72, Characterization of genes associated with adipose tissue is key to understanding the pathogenesis of obesity and developing treatments for this disorder. Differential gene expression in the adipose tissue has been described in adulthood but none studies have been developed on childhood. The purpose of this study was to compare gene expression in omental adipose tissue from obese prepubertal and normal weight children. We selected 5 obese (BMI adjusted for age and sex z score >2) and 6 normal weight children. RNA was extracted from omental adipose tissue biopsies and cRNA was hybridizated on the human genome U133 Plus 2.0 Arrays (Affymetrix®). Microarray experiments were performed for each sample, and selected group of gene expression values were confirmed with real-time RT-PCR in 10 obese and 10 normal weigth prepubertal children. 1276 genes were found to be differentially expressed at P<0.05. Of those differential genes, 201 were upregulated (Fc>2) and 42 were downregulated (Fc<-2). Genes involved in metabolic and signalling pathways were altered in childhood obesity. Keywords: disease state analysis