BUSCADOR RECOLECTA

ICOOP-CSIC, Peer reviewed

This dataset contains all data used in the analysis for the associated manuscript, as well as an R script which can be used to replicate the analysis.-- There are six files, including multispecies stable isotope data, cod sampling data, cod specific stable isotope data, cod meta data, cod positions and an R script for analysis, Foraging is a behavioural process and, therefore, individual behaviour and diet are theorized to covary. However, few comparisons of individual behaviour type and diet exist in the wild. We tested whether behaviour type and diet covary in a protected population of Atlantic cod, Gadus morhua. Working in a no-take marine reserve, we could collect data on natural behavioural variation and diet choice with minimal anthropogenic disturbance. We inferred behaviour using acoustic telemetry and diet from stable isotope compositions (expressed as δ13C and δ15N values). We further investigated whether behaviour and diet could have survival costs. We found cod with shorter diel vertical migration distances fed at higher trophic levels. Cod δ13C and δ15N values scaled positively with body size. Neither behaviour nor diet predicted survival, indicating phenotypic diversity is maintained without survival costs for cod in a protected ecosystem. The links between diet and diel vertical migration highlight that future work is needed to understand whether the shifts in this behaviour during environmental change (e.g. fishing or climate), could lead to trophic cascades, The Research Council of Norway, Award: CODSIZE 294926; Natural Sciences and Engineering Research Council; European Commission, Award: 793627; Spanish National Research Council, IF_ERC; Academy of Finland, Award: 317495; European Research Council, Award: 770884, file: cod_stable_isotopes.rdata; description: Cod specific stable isotopes data in the .rdata format.-- file: TVE2011Effort.csv; description: Sampling effort and locations in the Tvedestrand fjord.-- file: SI.rdata; description: stable isotope samples collected throughout the Tvedestrand foodweb.-- file: telem.rdata; description: Data collected during each cod sampling event, including recaptures.-- file: Allcodpositions.rdata; description: dataset "pos" contains a list of all cod positions used in the stable isotope anaylsis, Peer reviewed

[Descripción de los métodos utilizados para la recopilación/generación de datos] La evaluación de La Noche se realiza desde 2014 a través de una encuesta dirigida a asistentes e investigadores/as Para cada colectivo se ha diseñado un cuestionario específico, aunque algunas cuestiones son comunes. Ambos cuestionarios se han alojado en la web nocheinvestiga.es desarrollada íntegramente por IESA CSIC garantizando así la privacidad, seguridad y calidad de los datos. El contenido del cuestionario se ha mantenido durante todas las ediciones, si bien la técnica de recogida de datos se ha adaptado al tipo de actividad evaluada y los recursos disponibles. En el caso de los/as participantes distinguimos a continuación tres vías para recoger los cuestionarios según el tipo de actividad en el que han participado. - Micro-encuentros: se trata de aquellas actividades dirigidas a un número limitado de personas (microcharlas talleres, visitas guiadas, etc.) en las que los participantes facilitaron una dirección de correo electrónico para reservar plaza. A esa dirección se envió una invitación que da acceso online a la encuesta para evaluar la actividad. Entre el 5 y el 23 de octubre de 2023 se enviaron 1.202 invitaciones, con 3 recordatorios de respuesta, logrando una tasa de colaboración del 31,4% lo que se traduce en 371 cuestionarios. - Gran evento del 29 de septiembre: a los espacios que han concentrado gran cantidad de talleres y actividades durante La Noche del 29 de septiembre y han tenido más afluencia de público, se desplazó un equipo de encuestadores profesionales (2 por ciudad) que entrevistaron a 885 asistentes. - Actividades virtuales: la ficha explicativa de los vídeos alojados en la página web lanochedelosinvestiadores.es incluye un enlace a la encuesta online. Sin embargo, la colaboración de los asistentes por esta vía ha sido muy escasa y se han recogido solo 6 cuestionarios. En total, La Noche ha sido evaluada por 1.262 participantes de las ocho provincias andaluzas y por 1.334 investigadores e investigadoras pertenecientes a todas las instituciones que conforman el consorcio y a otras instituciones colaboradoras., [Modalidades de tratamiento de los datos] La grabación de datos es automática. En la depuración de los datos se han eliminado los casos que no habían cumplimentado el 75% del cuestionario, los casos con una duración de cumplimentación inferior a 2 minutos, los casos cumplimentados fuera Andalucía, Ceuta y Melilla. También se han eliminado los casos de participantes menores de 14 años en cumplimiento de la Ley 3/2018, de 5 de diciembre, de Protección de Datos Personales y garantía de los derechos digitales., El libro de códigos está disponible en español y en traducción al inglés., [ES] La Noche Europea de los Investigadores es un proyecto de divulgación científica en el que los investigadores/as muestran su trabajo a los ciudadanos de una forma lúdica así como su repercusión en la vida cotidiana. Se trata de una acción Marie Sklodowska Curie (MSCA), dentro del Programa Marco de Investigación e Innovación de la UE Horizonte Europa (2021-2027). Este evento se celebra simultáneamente en casi 400 ciudades europeas de 30 países desde 2005. En las ocho capitales andaluzas han participado más de 89.000 personas en las actividades realizadas en 2023 por más de 3.000 investigadores de las universidades públicas andaluzas y de los institutos del CSIC ubicados en la región. La evaluación de la Noche Europea de los Investigadores en Andalucía se ha realizado a través de una encuesta dirigida tanto a investigadores como al público en general. En total, han valorado el evento 1.262 asistentes y 1.334 investigadores/as., [EN] The European Researchers' Night is a public science outreach event, which displays the diversity of science and its impact on citizens' daily lives in fun, inspiring ways. It is a Marie Sklodowska Curie (MSCA) action, under the EU Framework Programme for Research and Innovation Horizon Europe (2021-2027). This event that takes place simultaneously in almost 400 European cities in 30 countries and has been held since 2005. In 2023, 89.000 visitors attended these events in the eight Andalusian cities. In addition, 3.000 researchers from Andalusian public universities and CSIC institutes located in the region have participated. The evaluation of The European Researchers' Night in Andalusia was carried out through a survey addressed to both researchers and the general public. In total, 1.262 attendees and 1.334 researchers evaluated the event., Proyecto financiado por el Programa Marco de Investigación Horizon Europe de la Comisión Europea, en el marco de las acciones Marie Sktodowska-Curie., Peer reviewed

Figure S1. 1H NMR spectra of urolithin D, urolithin C, urolithin M7, urolithin G, urolithin A, and isourolithin A in AcN-d3 and in DMSO-d6. *Signals from hydroxyl protons; Figure S2. 13C NMR spectrum (zoom from d = 110–115 ppm) of urolithin G in AcN-d3 and DMSO-d6. C2 and C7 were clearly separated in AcN-d3, while they appeared very close in the DMSO-d6 spectrum, Peer reviewed

12 files, This dataset consists of the GLODAPv2.2023 data product composed of data from 1108 scientific cruises covering the global ocean between 1972 and 2021. It includes full depth discrete bottle measurements of salinity, oxygen, nitrate, silicate, phosphate, dissolved inorganic carbon (TCO2), total alkalinity (TAlk), CO2 fugacity (fCO2), pH, chlorofluorocarbons (CFC-11, CFC-12, CFC-113, and CCl4), SF6, and various isotopes and organic compounds. It was created by appending data from 23 cruises to GLODAPv2.2022 (Lauvset et al., 2022, NCEI Accession 0257247). The data for salinity, oxygen, nitrate, silicate, phosphate, TCO2, TAlk, pH, CFC-11, CFC-12, CFC-113, CCl4, and SF6 were subjected to primary and secondary quality control. Severe biases in these data have been corrected for, and outliers removed. However, differences in data related to any known or likely time trends or variations have not been corrected for. These data are believed to be accurate to 0.005 in salinity, 1% in oxygen, 2% in nitrate, 2% in silicate, 2% in phosphate, 4 µmol kg-1 in TCO2, 4 µmol kg-1 in TAlk, and for the halogenated transient tracers and SF6: 5%, Funding Information: DOE, NOAA, NSF, EU agencies, Japan Climate Research Program, Peer reviewed

[EN] WETYDAS contains 12 TXT archives localized by their coordinates in NCAR grid; information include year, month, day and code of weathee type. [ES] WETYDAS consta de 12 archivos formato TXT geolocalizados por sus coordenadas en la malla NCAR; la información incluye el año, mes y día, así como el código del tipo de tiempo resultante., [EN] It has been applied the Jenkinson & Collison classification of Weather Types to Iberian Peninsula and Balearic Island by using the daily NCAR-NCEP grid surface pressure dataset (January-1950/December-2023). Grid resolution/2.5ºx2.5 lat/long) produces 12 series. Weather Types classification includes 8 directional pure, Anticyclonic and Cyclonic pure types, and combination of previous ones in the hybrid types. Non determines cases were spread homogeneously., [ES] Se ha aplicado la clasificación de tipos de tiempo (Weather Types) de Jenkinson y Collison a la malla de presiones diaria del reanálisis NCAR-NCEP (periodo Enero 1950-Diciembre 2023) correspondiente a la Península Ibérica y Baleares. Por la resolución de dicha malla (2.5º x 2.5º lat/long) el total de nodos de control es de 12. Los tipos de tiempo resultantes incluyen los 8 direccionales puros, Anticiclónico y Ciclónico puro, y la combinación de 8 tipos híbridos entre las categorías previas. Los casos indeterminados fueron distribuidos proporcionalmente entre las clases previas., No

10 files, f.MSV000089896/ccms_parameters/params.xml; f.MSV000089896/ccms_result/MCMMCM34021201_02_03_INK_BATCH_FDR_UNIGENE_PULPO_plus_Cephalopoda_Percolator.mzTab; f.MSV000089896/ccms_statistics/statistics.tsv; f.MSV000089896/peak/MCMMCM34021201.mzML; f.MSV000089896/peak/MCMMCM34021202.mzML; f.MSV000089896/peak/MCMMCM34021203.mzML; f.MSV000089896/raw/MCMMCM34021201.raw; f.MSV000089896/raw/MCMMCM34021202.raw; f.MSV000089896/raw/MCMMCM34021203.raw; f.MSV000089896/result/MCMMCM34021201_02_03_INK_BATCH_FDR_UNIGENE_PULPO_plus_Cephalopoda_Percolator.mzTab, Peer reviewed

7 páginas, 1 tabla, 5 figuras, Peer reviewed

Figure 1S. Comparison of total protein content in undigested Mexican and British chia (Salvia hispanica L.) ingredients and protein fractions. Abbreviations: DDF, degummed-defatted chia flour; PC, protein concentrate; Alb, albumin; Glo, globulin. Different lowercase letter within each sample group indicates statistical differences among the same chia samples from different locations (p < 0.05, Tukey test). Figure 2S. SEC profile at 214 nm for undigested Mexican and British chia (Salvia hispanica L.) samples and their digestates after gastric and intestinal digestion. A) degummed-defatted flour; B) protein concentrate; C) albumin, D) globulin. Abbreviations: M, Mexican chia samples; B, British chia samples; U, undigested; G, gastric phase; I, intestinal phase. Figure 3S. Kinetic evaluation of ROS production in Caco-2 cells and RAW264.7 macrophages in the presence of Mexican and British chia samples. A) ROS of Mexican chia samples in RAW264.7 Macrophages, B) ROS of British chia samples in RAW264.7 Macrophages, C) ROS of Mexican chia samples in Caco-2 cells D) ROS of British chia samples in Caco-2 cells. Abbreviations: DDF, degummed-defatted chia flour; PC, protein concentrate; Alb, albumin; Glo, globulin; M, Mexican chia samples; B, British chia samples., Table 1S. Peptide molecular weight distribution of chia (Salvia hispanica L.) samples and digestates., Peer reviewed

2 assembled transcriptomes and the respective raw reads: “Ovu1”: mRNA of almost hatched individuals (stage XX) and “Ovu2”: pooled mRNA of developmental stages VIII, XI, XV, XVIII, RNA-seq libraries were constructed with a Lexogen SENSE mRNA-Seq Library Prep Kit V2 and sequenced with an Illumina Hi-Seq 2500 generating paired-end, stranded 125 bp libraries resulting in 53,523,481 (ovu1) and 63,328,653 (ovu2) paired end reads. The overall transcriptome assembly follows the procedure performed by De Oliveira et al. (2016) [42]. The short‐read libraries were preprocessed using Trimmomatic v. 0.36 (ref. [43]) to remove known specific Illumina adapters from the paired‐end libraries (Illumina universal adapter). Filtering by quality and length was performed with a SLIDINGWINDOW:4:15 MINLEN:36. First and last nucleotides from reads with low quality score were clipped and the library file was converted into FASTA format using fq2fa from SeqKit version 0.11.0 (ref. [44]). Quality of the initial and filtered library was assessed with the software FastQC v.0.11.8 (ref. [45]) considering quality score of the bases, GC‐content, and read length. 13.33% (ovu1) and 16,23% (ovu2) of reads were excluded during the preprocessing procedure resulting in a total of 46,386,109 (ovu1) and 53,052,713 (ovu2) reads. The assemblies and all downstream analyses were conducted with a high‐quality and clean library. The filtered transcriptome was assembled into contiguous cDNA sequences with IDBA_tran v1.1.3 software (ref. [46]) using the default settings (except: −mink20 −maxk 80 −step5). The resulting assembly was assessed using the tool QUAST (available at: http://quast.bioinf.spbau.ru [47]). The number of contigs was 16,723 sequences (ovu1) and 18,551 (ovu2), Peer reviewed