BUSCADOR RECOLECTA

[ES] El conjunto de datos está dividido en tres subcarpetas, dentro de las cuales los datos se encuentran agrupados por especies: Eggs: Proporciona más de 900 imágenes en formato RAW en el rango visual del espectro visible de puestas completas sobre fondo negro, correspondientes a 14 especies distintas. Nests: Proporciona más de 800 imágenes de nidos de 6 especies distintas, tomadas en su entorno desde 1 metro de distancia y a 70 cm de altura. En este segundo conjunto de imágenes, se incluyen imágenes de los nidos tanto en el rango visible como en ultravioleta cercano. Incubating Behavior: Proporciona más de 950,000 imágenes en formato jpg correspondientes a secuencias de vigilancia del comportamiento de incubación de 5 especies distintas, junto con información de las condiciones ambientales., [EN] The dataset is divided into three subfolders, within which the data is grouped by species: Eggs: Provides over 900 images in RAW format within the visible spectrum of complete clutches on a black background, corresponding to 14 different species. Nests: Provides over 800 images of nests from 6 different species, taken in their environment from 1 meter away and at a height of 70 cm. This set includes images of the nests in both the visible range and near-ultraviolet. Incubating Behavior: Provides over 950,000 jpg images corresponding to surveillance sequences of incubation behavior from 5 different species, along with information on environmental conditions., [ES] Este conjunto de datos, generado en el marco del proyecto CAMNID: CGL2017-83518-P, Plan Nacional I+D+i, contiene información visual sobre huevos, nidos y comportamiento durante la incubación de diferentes especies de aves limícolas (shorebirds). Permite la realización de análisis de coloración y moteado, entre otros, para las especies en estudio. Asimismo, se proporcionan secuencias de imágenes de monitorización de diversos nidos de estas especies a lo largo de 3 horas (entre las 12:00 y las 15:00) junto con las condiciones ambientales durante la vigilancia. Esto permite la realización de estudios de los comportamientos de estas aves durante la incubación, abriendo la posibilidad de estudiar diversos mecanismos de mitigación de estrés térmico o las alternancias parentales macho/hembra durante la incubación en función de las condiciones ambientales, [EN] This dataset, generated within the framework of the CAMNID project: CGL2017-83518-P, Plan Nacional I+D+i, contains visual information on eggs, nests, and incubation behavior of various shorebird species. It enables color and spotting pattern analyses, among other studies, for the species included. Additionally, it provides image sequences monitoring various nests of these species over a 3-hour period (from 12:00 to 15:00) along with the environmental conditions during the surveillance. This allows for studies of the birds' behavior during incubation, opening the possibility to study mechanisms of thermal stress mitigation and male/female parental alternation during incubation based on environmental conditions., CAMNID PROJECT: Grant: CGL2017-83518-P, Plan Nacional I+D+i: EFECTOS DE LAS INVASIONES GEOLOGICAS DE ORIGEN ANTROPICO SOBRE LAS ESTRATEGIAS DE CAMUFLAJE DE NIDOS EN EL CHORLITEJO PATINEGRO CHARADRIUS ALEXANDRINUS, The dataset is comprissed of three folders. Eggs, Nests, and BehavioralMonitoring. ** Folder: Eggs: Photographs of shorebird eggs from a zenital view in raw format. - INFORMATION_TABLE.xlsx: Excel file with information about each picture in the Eggs folder subset. Image name can be used as unique identifier. - Folders corresponding to eggs of 14 different species. Species name corresponding to folder name. - Inside each folder, a single RAW format image file for each egg is provided, ** Folder: Nests: Zenital pictures of shorebirds nests in natural environments both in the Vis and UV wavelength ranges. - INFORMATION_TABLE.xlsx: Excel file with information about each picture in the Nests folder subset. Image name can be used as unique identifier. - Folders corresponding to 6 different species. Species name corresponding to folder name. - Inside each folder, two images per nest are provided. One for the visible wavelength range ({ImageName}_VIS.ARW) and another one for the UV range ({ImageName}_UV.ARW), **Folder: BehavioralMonitoring: Pictures showing the incubating activity of shorebird species. Additionally, environmental conditions are also provided. - README.txt: Readme file providing the description of each folder in the dataset - Folders corresponding to 5 shorebirds species being monitored. - Inside each species folder: ---subfolder: DATE_OF_OBSERVATION: | |---subfolder: DATALOGGERCODE (DTXX (XX from 0 to 9)) | |____FRAME_XXX_STD.jpg: XXXth Picture taken under normal conditions | |____YYY_ACT.jpg: YYYth Picture corresponding to a special activitity condition | |____DATA_LOGGER.txt: csv file containing enviromental conditions during this experiment. Updated every 100s | |____DTXX.gpx: GPS position of this observation (gpx standard format) | |____DTXX_GPS.txt: GPS position for this observation in txt format (the gpx file is not always available), Peer reviewed

The origin of the FtsZ/tubulin protein family was extremely relevant for life since these proteins are present in nearly all organisms, carrying out essential functions such as cell division or forming a major part of the cytoskeleton in eukaryotes. Therefore, investigating the early evolution of the FtsZ/tubulin protein family could reveal crucial aspects of the diversification of the three domains of life. In this study, we revisited the phylogenies of the FtsZ/tubulin protein family in an extensive prokaryotic diversity, focusing on the main evolutionary events that occurred during its evolution. We found evidence of its early origin in the last universal common ancestor since FtsZ was present in the last common ancestor of Bacteria and Archaea. In bacteria, ftsZ genes are genomically associated with the bacterial division gene cluster, while in archaea, ftsZ duplicated prior to archaeal diversification, and one of the copies is associated with protein biosynthesis genes. Archaea have expanded the FtsZ/tubulin protein family with sequences closely related to eukaryotic tubulins. In addition, we report novel CetZ-like groups in Halobacterota and Asgardarchaeota. Investigating the C-termini of prokaryotic paralogs basal to eukaryotic tubulins, we show that archaeal CetZ, as well as the plasmidic TubZ from Firmicutes, most likely originated from archaeal FtsZ. Finally, prokaryotic tubulins are restricted to Odinarchaeaota and Prosthecobacter species, and they seem to belong to different molecular systems. However, their phylogenies suggest that they are closely related to α/β-tubulins pointing to a potential ancestrality of these eukaryotic paralogs of tubulins, Peer reviewed

able S1: Species, sample, coverage, non-conversion rate (%), and average DNA methylation levels (mCG/CG) for all datasets generated in this study. Table S2: Genomic location of all differentially methylated regions (DMRs) identified in the medaka genome. Table S3: DMR-linked genes in zebrafish and medaka genomes., Peer reviewed

Supplementary Figure 1. GFP+ cells engraftment and mFVIII production correlation in transplanted mice. Supplementary Figure 2. Bleeding assay in newborn HA following transplantation. Supplementary Figure 3. Bleeding assay in adult HA following transplantation. Supplementary table 1. List of antibodies used for immunofluorescence and flow cytometry. Supplementary table 2. Engraftment (% GFP+ cells) and correction (% mFVIII activity) levels in plasma of newborn mice according to BU dosage and cell transplantation., Peer reviewed

Circulating antibody-secreting cells are present in the peripheral blood of healthy individuals reflecting the continued activity of the humoral immune system. Antibody-secreting cells typically express CD27. Here we describe and characterize a small population of antibody-secreting class switched CD19+CD43+ B cells that lack expression of CD27 in the peripheral blood of healthy subjects. In this study, we characterized CD27-CD43+ cells. We demonstrate that class-switched CD27-CD43+ B cells possess characteristics of conventional plasmablasts as they spontaneously secrete antibodies, are morphologically similar to antibody-secreting cells, show downregulation of B cell differentiation markers, and have a gene expression profile related to conventional plasmablasts. Despite these similarities, we observed differences in IgA and IgG subclass distribution, expression of homing markers, replication history, frequency of somatic hypermutation, immunoglobulin repertoire, gene expression related to Toll-like receptors, cytokines, and cytokine receptors, and antibody response to vaccination. Their frequency is altered in immune-mediated disorders., Peer reviewed

Histological sections were incubated with Aqp1ab2-Nt and Aqp1ab2_v1 antisera preabsorbed with the immunizing peptides, indicating the specificity of the antibodies. Abbreviations: o, oocyte; y, yolk globule; gv, germinal vesicle; ve, vitelline envelope; cp, capillary; ca, cortical alveoli. Scale bars, 10 μm., Peer reviewed

Peer reviewed

[Introduction]: Drought-associated tree mortality has been increasing worldwide since the last decades, impacting structure and functioning of forest ecosystems, with implications for energy, carbon and water fluxes. However, the understanding of the individual vulnerability to drought-induced mortality is still limited., [Methods]: We aimed to identify the factors that triggered the mortality of the widely distributed Pinus sylvestris L. in an extensive forest area in central Spain. We compared radial growth patterns in pairs of alive and recently dead individuals that co-occur in close proximity and present similar age and size, thereby isolating the effects of size and environment from the mortality process. Temporal dynamics of growth, growth synchrony, and growth sensitivity to water availability (precipitation minus potential evapotranspiration) were compared between alive and recently dead trees., [Results and discussion]: Over the last 50 years, although we did not detect significant differences in growth between alive and dead trees, an increase in the growth synchrony and sensitivity to water availability (i.e. slope of the climatic water balance in the growth model) was observed in all trees as drought intensity increased. 20 years before mortality, dead individuals showed lower growth synchrony and growth sensitivity to water availability than alive ones, without significant differences in growth. Recorded reduction in growth synchrony and growth sensitivity to water availability in dead trees suggests a decoupling between tree growth and climate, which could increase the risk of hydraulic failure and/or carbon starvation under increasingly arid conditions. Thus, the use of reduced growth sensitivity to water availability as potential early-warning signal of tree mortality, together with reduced growth synchrony, should be further explored, particularly in pine species in seasonally dry areas., Peer reviewed

[Background]: Little is known about whether the overlap syndrome (OS) combining features of chronic obstructive pulmonary disease (COPD) and sleep apnea-hypopnea syndrome increases the risk of stroke associated with COPD itself., [Methods]: We prospectively studied 74 COPD patients and 32 subjects without lung disease. Spirometry and cardiorespiratory polygraphy were used to assess the pulmonary function of the study population and ultrasound measurements of intima media thickness (IMT) as well as the volume of plaques in both carotid arteries were also evaluated., [Results]: Polygraphic criteria of OS were met in 51% of COPD patients. We found that 79% of patients with OS and 50% of COPD patients without OS had atherosclerotic plaques in the left carotid artery (p = 0.0509). Interestingly, the mean volume of atherosclerotic plaques was significantly higher in the left carotid artery of COPD patients with OS (0.07 ± 0.02 ml) than in those without OS (0.04 ± 0.02 ml, p = 0.0305). However, regardless of the presence of OS, no significant differences were observed in both presence and volume of atherosclerotic plaques in the right carotid artery of COPD patients. Adjusted-multivariate linear regression revealed age, current smoking and the apnea/hypopnea index (OR = 4.54, p = 0.012) as independent predictors of left carotid atherosclerotic plaques in COPD patients., [Conclusions]: This study suggests that the presence of OS in COPD patients is associated with larger left carotid atherosclerotic plaques, indicating that OS might be screened in all COPD patients to identify those with higher risk of stroke., Peer reviewed

Additional file 13: Supplementary Table S4. tRNA fragment reads in Control (wt) and METTL1 KO cells., Consejo Superior de Investigaciones Científicas (España), Peer reviewed