Resultados de investigación

1 table., Additional file 6: Table S4. Deviance information criterion (DIC) values computed for each microbial trait with the normal linear mixed model and the zero-inflated Poisson model., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Horizon 2020 Framework Programme Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

1 table., Additional file 7: Table S5. Mean (standard deviation) of Bayes factors and cage variance ratio estimates for OTU that are influenced by cage effects and adjusted with the normal LMM. Table S6. Mean (standard deviation) of Bayes factor and cage variance ratio estimates for OTU that are influenced by cage effect and adjusted with the ZIP model. Tables S7. Bayes factors, marginal posterior means (standard deviations) of cage variance ratio for genera and alpha-diversity indices that are influenced by cage effects., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Horizon 2020 Framework Programme Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

1 table., Additional file 8: Table S8. Bayes factors, marginal posterior means and standard deviations of heritability estimates for OTU under genetic control together with the associated probability of these estimates being higher than 0.10. The frequency among the rabbit samples and the taxonomic assignment are also provided for each OTU., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Horizon 2020 Framework Programme Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Additional file 9: Table S9. Bayes factors, marginal posterior means and standard deviations of litter variance ratio for OTU influenced by litter effects together with the associated probability of these estimates being higher than 0.10. The frequency among the rabbit samples and the taxonomic assignment are also provided for each OTU., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Horizon 2020 Framework Programme Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Peer reviewed

1 table., Additional file 1: Table S1. Posterior means, posterior medians, 95% highest posterior density intervals (HPD95%) structural coefficients different from zero (in g/d, CSS-normalized OTU units) that had an effect on the average daily gain of growing rabbits fed ad libitum and their assignment at the lowest taxonomic level. Table S2. Posterior means, posterior medians, 95% highest posterior density intervals (HPD95%) structural coefficients different from zero (in g/d, CSS-normalized OTU units) that had an effect on the average daily gain of growing rabbits under restricted feeding and their assignment at the lowest taxonomic level., Peer reviewed

28 days old root system were collected from soil, quickly washed in water and flash-frozen. Experiment with a bifactorial design. Factor one is the genotype, which include WT (Col-0) and 35S:BRL3. Factor two is the condition, which include control (Properly watered) and 5 days of drought (water-hold) conditions. 3 Biological replicates were collected per each genotype and condition. -- Organism: Arabidopsis thaliana. -- Experiment type: Expression profiling by high throughput sequencing., Resources available on the publisher's site: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE119382, Drought is the primary cause of global agricultural losses and represents a major threat to worldwide food security. Currently, plant biotechnology stands out as the most promising strategy to increase crop growth in rain-fed conditions. The main mechanisms underlying drought resistance have been uncovered by studies of plant physiology and by engineering crops with drought-resistant genes. However, plants with enhanced drought resistance usually display lower levels of growth, highlighting the need to search for novel strategies capable of uncoupling drought resistance from growth. Here, we show that the brassinosteroid family of receptors, in addition to promoting growth, guides phenotypic adaptation to a great variety of drought stress traits analyzed herein. Whilst mutations in the ubiquitously localized BRI1 receptor pathway show an enhanced drought resistance at the expense of plant growth, we found that vascular-enriched BRL3 receptors confer drought tolerance without penalizing overall growth. Systematic analyses reveal that upon drought stress the BRL3 receptor pathway triggers the synthesis and mobilization of osmoprotectant metabolites, mainly proline and sugars. This preferentially occurs in the vascular tissues of the roots and favors overall plant growth. Altogether, our results uncover a new role for the spatial control of BR signaling in drought tolerance, and offer a novel strategy to address food security issues in an increasingly water-limited climate., Peer reviewed

8 pages. -- Table S1: passport information for all the samples analyzed in the study, including accession number and sample name from the reference publication. The field “group” is assigned based on the DAPC analyses. -- Table S2: Patterson’s D (ABBA-BABA), all tested triplets. Significant results are shown in bold. As per D-suite, the populations are always ordered to infer gene flow between P2 and P3 (D>0 ). -- Fig S1: Principal Component Analysis of the modern (whole genome) dataset, prior to group determination: two outliers are identified and excluded from further analysis, while major groups are identified. -- Fig S2: xValDAPC showing the best number of PCs to retain for DAPC analysis, based on the highest proportion of successful outcome prediction (0-1). -- Fig S3: Neighbor Joining analysis with 100 bootstrap replicates of the modern (whole genome) dataset. Bootstrap values are not shown (all nodes have bootstrap more than 90). -- Fig S4: Admixture plots from K=2 to K=6, modern whole genome dataset. -- Fig S5 (a-d): TreeMix residuals., Peer reviewed

1 figure., The causal agent of bacterial wilt, Ralstonia pseudosolanacearum, can cause significant economic losses during tobacco production. Metabolic analyses are a useful tool for the comprehensive identification of plant defense response metabolites. In this study, a gas chromatography-mass spectrometry (GC-MS) approach was used to identify metabolites differences in tobacco xylem sap in response to R. pseudosolanacearum CQPS-1 in two tobacco cultivars: Yunyan87 (susceptible to R. pseudosolanacearum) and K326 (quantitatively resistant). Metabolite profiling 7 days post inoculation with R. pseudosolanacearum identified 88 known compounds, 42 of them enriched and 6 depleted in the susceptible cultivar Yunyan87, while almost no changes occurred in quantitatively resistant cultivar K326. Putrescine was the most enriched compound (12-fold) in infected susceptible tobacco xylem, followed by methyl-alpha-d-glucopyranoside (9-fold) and arabinitol (6-fold). Other sugars, amino acids, and organic acids were also enriched upon infection. Collectively, these metabolites can promote R. pseudosolanacearum growth, as shown by the increased growth of bacterial cultures supplemented with xylem sap from infected tobacco plants. Comparison with previous metabolic data showed that beta-alanine, phenylalanine, and leucine were enriched during bacterial wilt in both tobacco and tomato xylem., Peer reviewed

1. IR spectra S1 2. NMR spectra S2 3. Multistage mass spectrometry (MSn) S5 4. TGA/DTA and thermolytic results S9 5. Crystal data, Tables and Figures S10 6. Further computational details S16 7. Reference S21, Peer reviewed