Resultados totales (Incluyendo duplicados): 33785
Encontrada(s) 3379 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311662
Dataset. 2022

IMAGE_2_MOLECULAR CHARACTERIZATION, TARGETING AND EXPRESSION ANALYSIS OF CHLOROPLAST AND MITOCHONDRION PROTEIN IMPORT COMPONENTS IN NICOTIANA BENTHAMIANA.TIF

  • Saiz-Bonilla, María
  • Martín Merchán, Andrea
  • Pallás, Vicente
  • Navarro, José A.
Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311662
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311662
HANDLE: http://hdl.handle.net/10261/311662
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311662
PMID: http://hdl.handle.net/10261/311662
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311662
Ver en: http://hdl.handle.net/10261/311662
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311662

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311663
Dataset. 2022

SUPPORTING INFORMATION FOR SENSORIMOTOR EFFECTS OF PLASTICITY-INDUCING PERCUTANEOUS PERIPHERAL NERVE STIMULATION PROTOCOLS: A BLINDED, RANDOMIZED CLINICAL TRIAL

  • Beltrá, P.
  • Ruiz-del-Portal, I.
  • Ortega, F. J.
  • Valdesuso, R.
  • Delicado-Miralles, Miguel
  • Velasco, Enrique
Supporting Information: Figures and Tables. Supplementary Material., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311663
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311663
HANDLE: http://hdl.handle.net/10261/311663
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311663
PMID: http://hdl.handle.net/10261/311663
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311663
Ver en: http://hdl.handle.net/10261/311663
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311663

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311664
Dataset. 2022

IMAGE_1_MOLECULAR CHARACTERIZATION, TARGETING AND EXPRESSION ANALYSIS OF CHLOROPLAST AND MITOCHONDRION PROTEIN IMPORT COMPONENTS IN NICOTIANA BENTHAMIANA.TIF

  • Saiz-Bonilla, María
  • Martín Merchán, Andrea
  • Pallás, Vicente
  • Navarro, José A.
Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311664
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311664
HANDLE: http://hdl.handle.net/10261/311664
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311664
PMID: http://hdl.handle.net/10261/311664
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311664
Ver en: http://hdl.handle.net/10261/311664
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311664

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311665
Dataset. 2022

DATASHEET_1_MOLECULAR CHARACTERIZATION, TARGETING AND EXPRESSION ANALYSIS OF CHLOROPLAST AND MITOCHONDRION PROTEIN IMPORT COMPONENTS IN NICOTIANA BENTHAMIANA.DOCX

  • Saiz-Bonilla, María
  • Martín Merchán, Andrea
  • Pallás, Vicente
  • Navarro, José A.
Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311665
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311665
HANDLE: http://hdl.handle.net/10261/311665
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311665
PMID: http://hdl.handle.net/10261/311665
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311665
Ver en: http://hdl.handle.net/10261/311665
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311665

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668
Dataset. 2022

GLIAL-SECRETED NETRINS REGULATE ROBO1/RAC1-CDC42 SIGNALING THRESHOLD LEVELS DURING DROSOPHILA ASYMMETRIC NEURAL STEM AND PROGENITOR CELL DIVISION [DATASET]

  • Torres Jurado, Ana de
  • Manzanero-Ortiz, Sandra
  • Carmena, Ana
Figures, original raw data, of the above paper., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668
HANDLE: http://hdl.handle.net/10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668
PMID: http://hdl.handle.net/10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668
Ver en: http://hdl.handle.net/10261/311668
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311668

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311672
Dataset. 2022

SUPPLEMENTARY MATERIAL FROM NETWORK-LEVEL CONTAINMENT OF SINGLE-SPECIES BIOENGINEERING

  • Maull, Victor
  • Solé, Ricard V.
Ecological systems are facing major diversity losses in this century due to Anthropogenic effects. Habitat loss, overexploitation of resources, invasion and pollution are rapidly jeopardizing the survival of whole communities. It has been recently suggested that a potential approach to flatten the curve of species extinction and prevent catastrophic shifts would involve the engineering of one selected species within one of these communities. Such possibility has started to become part of potential intervention scenarios to preserve biodiversity. Despite its potential, very little is known about the actual dynamic responses of complex ecological networks to the introduction of a synthetic strains derived from a resident species. In this paper, we address this problem by modelling the response of a community to the addition of a synthetic strain derived from a member of a stable ecosystem. We show that the community interaction matrix largely limits the spread of the engineered strain, thus suggesting that species diversity acts as an ecological firewall. The implications for future scenarios of ecosystem engineering are outlined.This article is part of the theme issue ‘Ecological complexity and the biosphere: the next 30 years’., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311672
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311672
HANDLE: http://hdl.handle.net/10261/311672
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311672
PMID: http://hdl.handle.net/10261/311672
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311672
Ver en: http://hdl.handle.net/10261/311672
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311672

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311674
Dataset. 2022

SUPPORTING INFORMATION FOR UNCOUPLING AXON GUIDANCE AND NEURONAL MIGRATION IN ROBO3-DEFICIENT INFERIOR OLIVARY NEURONS

  • Moreno-Bravo, Juan Antonio
  • Rappeneau, Quentin
  • Roig-Puiggros, Sergi
  • Sotelo, Constantino
Supplementary movie S1: 3D time-course analysis of IO neuron migration in wildtype mice. Supplementary movie S2: 3D time-course analysis of IO neuron migration in Robo3 knockout mice. Supplementary movie S3: Morphology of Tomato+/Foxp2+ IO neurons in a E13 Ptf1a:CreERT2 ;RosaTom embryo. Supplementary movie S4: Morphology of Tomato+/Foxp2+ IO neurons in a E13 Ptf1a:CreERT2; Robo3-/- ;RosaTom embryo., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311674
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311674
HANDLE: http://hdl.handle.net/10261/311674
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311674
PMID: http://hdl.handle.net/10261/311674
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311674
Ver en: http://hdl.handle.net/10261/311674
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311674

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311691
Dataset. 2022

RAPID FLUCTUATIONS OF THE SUBSURFACE CHLOROPHYLL MAXIMUM IN RESPONSE TO WIND FORCING IN A LONG, NARROW BAY - SUPPLEMENTARY INFORMATION

  • Broullón, Esperanza
  • Franks, Peter J. S.
  • Fernández-Castro, B.
  • Gilcoto, Miguel
  • Fuentes-Lema, A.
  • Mouriño-Carballido, Beatriz
  • Pérez-Lorenzo, María
  • Fernández, Emilio
Dataset used for analyses in the publication "Rapid Fluctuations of the Subsurface Chlorophyll Maximum in Response to Wind Forcing in a Long, Narrow Bay", Project REMEDIOS (CTM2016-75451-C2-1-R) to BM-C from the Spanish Ministry of Economy and Competitiveness, No

DOI: http://hdl.handle.net/10261/311691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311691
HANDLE: http://hdl.handle.net/10261/311691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311691
PMID: http://hdl.handle.net/10261/311691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311691
Ver en: http://hdl.handle.net/10261/311691
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311691

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311692
Dataset. 2022

SUPPLEMENTAL INFORMATION FOR ACTIVATION-INDUCED CHROMATIN REORGANIZATION IN NEURONS DEPENDS ON HDAC1 ACTIVITY

  • Grabowska, Agnieszka
  • Sas-Nowosielska, Hanna
  • Wojtas, Bartosz
  • Holm-Kaczmarek, Dagmara
  • Januszewicz, Elzbieta
  • Yushkevich, Yana
  • Czaban, Iwona
  • Trzaskoma, Pawel
  • Krawczyk, Katarzyna
  • Gielniewski, Bartlomiej
  • Martin-Gonzalez, Ana M.
  • Filipkowski, Robert Kuba
  • Olszyński, Krzysztof Hubert
  • Bernas, Tytus
  • Szczepankiewicz, Andrzej A.
  • Śliwińska, Małgorzata Alicja
  • Kanhema, Tambudzai
  • Bramham, Clive R.
  • Bokota, Grzegorz
  • Plewczynski, Dariusz
  • Wilczyński, Grzegorz Marek
  • Magalska, Adriana
Supplemental information: Document S1. Figures S1–S7. Table S1. GO analysis. Table S2. KEGG cLTP down-regulated. Table S3. KEGG cLTP up-regulated. Document S2. Article plus supplemental information., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311692
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311692
HANDLE: http://hdl.handle.net/10261/311692
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311692
PMID: http://hdl.handle.net/10261/311692
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311692
Ver en: http://hdl.handle.net/10261/311692
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311692

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311707
Dataset. 2022

DATA AND CODE FOR: CENTRALIZED ADMISSIONS, AFFIRMATIVE ACTION AND ACCESS OF LOW-INCOME STUDENTS TO HIGHER EDUCATION

  • Mello, Úrsula
These files contain the programs and the public data for the journal article: "Centralized Admissions, Affirmative Action and Access of Low-income Students to Higher Education", American Economic Journal: Economic Policy., Barcelona Graduate School of Economics (Severo Ochoa Program for Centers of Excellence in RD - SEV2015-0563, CEX2019-000915-S); Institute for Economic Analysis (2017 SGR 1359 Secretaria d’Universitats i Recerca-Generalitat de Catalunya), With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2019-000915-S)., Peer reviewed

DOI: http://hdl.handle.net/10261/311707
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311707
HANDLE: http://hdl.handle.net/10261/311707
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311707
PMID: http://hdl.handle.net/10261/311707
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311707
Ver en: http://hdl.handle.net/10261/311707
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311707

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