Resultados de investigación

1 figure., A) Western blot showing presence of fusion proteins in extracts used in lipid blot experiments in Fig 5A. Arrows indicate locations of full-length SAUR63:YFP:HA and truncated SAUR6326-142:YFP:HA fusion proteins (upper arrow) and SAUR631-25:YFP:HA N-terminal domain fusion protein (lower arrow). B) Longer exposures of two lipid blots from Fig 5A. C) Mock experiment in which extracts were incubated for 70 minutes in protein extraction buffer at the indicated temperatures, and then run on a gel for western blots. For both SAUR63:YFP:HA and SAUR6326-142:YFP:HA, similar amounts of protein are present after incubation at -20 C or after incubation at 22 C, as during the lipid blot binding experiment., Peer reviewed

1 figure., A)PEST:SAUR63NAAIRS:CerFP:HA lines grown on plates with estradiol (all genotypes) or without estradiol (wild-type Columbia and PEST:SAUR63:CerFP:HA only). S2 Table indicates amino acids changed in each mutant and root tortuosity index measurement data from this and one replicate experiment. Scale bar, 1 cm. B) Western blots of total protein in estradiol-induced PEST:SAUR63NAAIRS:CerFP:HA lines using α-HA antibody., Peer reviewed

The supporting information: Figure S1: Sapogenin content (g/100 g) and extraction yield (%) of extracts obtained at different molarities of HCl by microwave hydrolysis at 140 °C for 30 min. Standard deviations are indicated by error bars. Mean values with different letters are significantly different (p ≤ 0.05); Figure S2: Lipase inhibition (%) and sapogenin content (g/100 g) of extracts obtained at different molarities of HCl by microwave hydrolysis at 140 °C for 30 min. Standard deviations are indicated by error bars. Mean values with different letters are significantly different (p ≤ 0.05); Figure S3: DPPH inhibition (%) and sapogenin content (g/100 g) of extracts obtained at different molarities of HCl by microwave hydrolysis at 140 °C for 30 min. Standard deviations are indicated by error bars. Mean values with different letters are significantly different (p ≤ 0.05); Table S1: Sapogenin profile of sapogenin-rich extracts produced from a commercial saponin-rich fenugreek extract under conventional hydrolysis (100 °C, 60 min) or microwave-assisted acid hydrolysis (MAAH, 30 min) at different temperatures (100, 120, 130 and 140 °C); Table S2: Sapogenin profile of sapogenin-rich extracts produced from a commercial saponin-rich fenugreek extract under conventional hydrolysis (100 °C, 60 min) or microwave-assisted acid hydrolysis (MAAH, 140 °C) at different times (10, 20, 30 and 40 min)., Peer reviewed

Supplementary data 1., Peer reviewed

1 figure., A-D)P35S:SAUR63:YFP:HA. E-H)P35S:SAUR6326-142:YFP:HA. I-L)P35S:SAUR63m9:YFP:HA. M-P)P35S:SAUR63m13:YFP:HA. Q-T)P35S:SAUR63m15:YFP:HA. Shown are fluorescence confocal microscopy images of YFP (green, A,E,I,M,Q), FM4-64 membrane staining (magenta, B,F,J,N,R), and both channels together (C,G,K,O,S) with vertical yellow lines indicating locations of quantitation of fluorescence intensity signals, scaled to the maximum signal along the line (D,H,L,P,T). Image color channel brightnesses were adjusted for visibility. Panels A-D are the same as in Fig 8. Scale bar, 20 μm., Peer reviewed

The supporting information: Table S1: Analysis of variance for TPC for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S2: Analysis of variance for AChE for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S3: Analysis of variance for LOX for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S4: Analysis of variance for ROS for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S5: PLE conditions, desirability, predicted response values at the optimum predicted by the model and experimental response values for the selected optimum (50% ethanol at 180 °C). Table S6: Multiple response optimization. Table S7: MS1 (m/z) and RT (min) of the internal standards used. Table S8: Abundance levels of tentatively identified metabolites in thinned peach PLE extracts. Figure S1: Response surfaces for each response variable and their corresponding standardized Pareto charts: A, TPC (mg GAE/mL); B, IC50 AChE (µg/mL); C, IC50 LOX (µg/mL); D, IC50 ROS (µg/mL). Figure S2: Desirability response surface used to optimize all response variables., Peer reviewed

Here are published: The data sets used for the analyses and the most divergent loci between S. rosae and S. sertorius., PID2019-107078GB-I00/AEI/10.13039/501100011033 (Spanish Agencia Estatal de Investigación) 2017-SGR-991 (Generalitat de Catalunya) BES-2017–080641 (MINECO/AEI/FSE), Peer reviewed

Results of quantitative real-time PCR after in vitro fermentation at 10 and 24 h and statistical test data., Peer reviewed

The supporting information: Figure S1: The role of EWH in NF-κB-induced impairment on acetylcholine (ACh) relaxation in MRA of DOCA-salt rats; Figure S2: The role of EWH in NO-mediated acetylcholine (ACh)-induced relaxation in the aorta of DOCA-salt rats; Figure S3: NO production is recovered by EWH treatment in aortic sections of DOCA-salt rats., Peer reviewed

The supporting information: Figure S1: (a) Systolic blood pressure from WKY, SHR-Ctrl and SHR-SYNB animals (Mean + S.E.M.; n = 11–14 animals each group) expressed in mm Hg. One-way ANOVA, followed by a Tukey post-hoc test was used as a statistical analysis. * p < 0.05 WKY vs. SHR-Ctrl; # p < 0.05 SHR-Ctrl vs. SHR-SYNB. + p < 0.05 WKY vs. SHR-SYNB. (b) EFS-induced NO release in mesenteric segments from WKY, SHR-Ctrl and SHR-SYNB animals (Mean + S.E.M.; n = 11–14 animals each group) expressed in arbitrary fluorescence units (A.F.U.)/mg tissue. One-way ANOVA, followed by a Tukey post-hoc test was used as a statistical analysis. * p < 0.05 WKY vs. SHR-Ctrl; # p < 0.05 SHR-Ctrl vs. SHR-SYNB. + p < 0.05 WKY vs. SHR-SYNB (c) Analysis of the functional role of neuronal NO on EFS-induced vasoconstriction by preincubation with the unspecific nitric oxide synthase (NOS) inhibitor L-NAME, in mesenteric arteries WKY rats. Results (mean ± S.E.M.) are expressed as a percentage of previous tone induced by KCl. n = 6 segments from different animals in each ex-perimental group., Peer reviewed