BUSCADOR RECOLECTA

Supplementary Information: Additional file 1: Supplementary Figure S1. Photorhabdus luminescens incubated with the KC-12 probe during 70 h. Supplementary Note 1. Taxonomic Appendix. Supplementary Figure S2. Hybridization of cells from nudibranch skin, gut, gills and gonads. Supplementary Figure S3. KEGG modules of Ca. D. californiensis and medium quality Ca. Tethybacterales MAGs from sponges. Supplementary Figure S4. Sulfur and nitrogen metabolism of Ca. D. californiensis and medium quality Ca. Tethybacterales MAGs from sponges. Supplementary Figure S5. ABC transporters of Ca. D. californiensis and medium quality Ca. Tethybacterales MAGs from sponges. Supplementary Figure S6. Phylogenetic tree of 16S rDNA gene sequences from public datasets. Supplementary Figure S7. Proportion of reads with >92% sequence similarity to Ca. D. californiensis in other nudibranchs. Supplementary Figure S8. Heatmap showing relative abundances of top ASVs across all 16S rDNA amplicon samples. Supplementary Figure S9. Core microbiome of D. fulva mantle. Supplementary Figure S10. ASVs determining the ordination of samples in the PCA anlaysis of nudibranch microbiome samples. Supplementary Figure S11. Phylogenetic tree of full length 16S rDNA gene sequences from nudibranch skin and mucus obtained by Sanger sequencing. Supplementary Figure S12. Ca. D. californiensis betalactone gene cluster compared to the best antiSMASH matches. Supplementary Figure S13. Similarity of the genes in the Ca. D. californiensis betalactone gene cluster with the 'nr' database of NCBI. Supplementary Figure S14. Similarity of the genes in the Ca. D. californiensis betalactone gene cluster with other members of the Ca. Tethybacterales order. Supplementary Figure S15. Biosynthetic pathway comparison to known betalactone natural product pathways. Supplementary Figure S16. NMR analysis of extracts from D. fulva nudibranchs. Supplementary Figure S17. Quantification of the dcbD expression in the CRAGE strains and nudibranch samples. Supplementary Figure S18. Testing of the CRAGE strain Aeromonas piscicola for betalactone expression induced by IPTG. Supplementary Figure S19. Feature-based molecular network identifying metabolite production in the A. piscicola DCB+ CRAGE strain. Additional file 2: Supplementary Table S1. Overview of the collected Doriopsilla fulva nudibranchs and methods applied for their analysis. Supplementary Table S2. Synthetic building blocks and PCR primer sequences used for engineering CRAGE strains. Supplementary Table S3. Details on CRAGE strains genomes. Supplementary Table S4. Assemblies of multi-cell sorts. Supplementary Table S5. SNPs and indels in the multi-cell sorts sequence data. Supplementary Table S6. 16S rDNA amplicon sequencing runs from IMNGS containing reads with >92% similarity to Ca. D. californiensis. Supplementary Table S7. 16S rDNA amplicon sequencing runs from previous nudibranchs microbiome studies containing reads with >92% similarity to Ca. D. californiensis. Supplementary Table S8. Collected nudibranch samples and the details on 16S rDNA amplicon Illumina sequencing runs. Additional file 3. Supplementary File S1. DCB antiSMASH output file., Peer reviewed

Inscripción en castellano situada en la fachada sur de la iglesia, a la derecha de la puerta. Grabada en un sillar, con caracteres regulares y bien trazados, más grandes para las tres primeras líneas, y más pequeñas en la última que contiene la datación., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

La inscripción recorre el friso de la portada principal de la iglesia, con un escudo en el centro. Hace referencia a la reconstrucción del edificio, con el año, ilegible, por Juan Gil de la Parra, su "rector"., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

Lápida central de un grupo de tres, en piedra, situada en el extremo derecho del atrio de la Iglesia de Nuestra Señora de la Asunción. Su ubicación original estaba en el prebisterio., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

Losa izquierda del grupo de tres que se encuentra en el extremo derecho del atrio de la Iglesia de la Asunción, Almodóvar del Campo., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

El epitafio propiamente dicho contiene dos inscripciones: una (A), en castellano en un cuadro rectangular de fondo negro, enmarcado por una moldura; otra, en latín (B), es un dístico elegíaco con el hexámetro dactílico en el lado superior de la moldura y el pentámetro en el inferior, en ambos casos con letra de menos tamaño que la inscripción en castellano y con texto inspirado en San Jerónimo, In Psalmum XXXI ad Neophytos, 51. el cuadro está colgado del muro occidental de la Capilla del Bautismo., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

11 pages. -- Fig. S1: Sample 4 (Si-He-RF/fl) was fabricated in a tilted geometry chamber for complementary experiments of ex-situ annealing under inert gas flow conditions. Deposition conditions and IBA characterization are summarized. -- Fig. S2: EBS For 4He depth profiling. -- Fig. S3a: ERDA for H depth profiling. -- Fig. S3b: RBS for Si depth profiling. -- Fig. S4: NRA for O and C depth profiling (except sample 3). -- Fig. S5: NRA for 3He, C and O depth profiling (in sample 3). -- Fig. S6: Summary of depth resolutions and detection limits. -- Fig. S7a: Study of silicon layers fabricated by magnetron sputtering (MS) with 4He in DC or RF operation mode. Pore (or trapped gas nanobuble) size and aspect ratio distribution histograms from TEM cross-sectional images. Sample 1: Si-He-RF (top). Sample 2: Si-He-DC (bottom). -- Fig. S7b: Study of silicon layers fabricated by magnetron sputtering (MS) with 3He in RF operation mode. Pore (or trapped gas nanobuble) size and aspect ratio distribution histograms from TEM cross-sectional images. Sample 3: Si-3He-RF. -- Fig. S8: Representative XRD (grazing incidence) diffractogram of sample 2 (Si-He-DC). Silicon is amorphous in all the as prepared Si-He samples. -- Fig. S9: TEM cross-section study of sample 1 (Si-He-RF). Low magnification image at bottom-left. High magnification images at indicated zones. A dense thin layer at the first stage of film growth is visualized at bottom-right., Peer reviewed

La inscripción recorre un friso situado en la reja de la capilla de la inmaculada, en el lado de la nave. La reja es obra de Juan Francés, rejero de la Catedral de Toledo. El texto latino recoge la última perícopa del Ave Regina Coelorum., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

Additional file 1: Supplementary Materials and Methods and Supplementary figures S1, S2, S3, S4, S5, S6, S7, S8. Additional file 2: Table S1. List of differentially expressed genes in bulk RNA-seq. Additional file 3: Table S2. Quantitative PCR validation of top differentially-expressed genes. Additional file 4: Table S3. Gene Ontology analysis for biological process for up- and downregulated genes in bulk RNA-seq. Additional file 5: Table S4. Gene Ontology analysis for cellular component for up- and downregulated genes in bulk RNA-seq. Additional file 6: Table S5. Clusters of protein-protein interactions (PPI) for the corresponding proteins of up- and downregulated genes in bulk RNA-seq. Additional file 7: Table S6. List of population marker genes used for supervised clustering analysis of snRNA-seq data. Additional file 8: Table S7. Quantification of cells belonging to each of the major population clusters in snRNA-seq data. Additional file 9: Table S8. List of differentially expressed genes in major sn-RNAseq clusters. Additional file 10: Table S9. Gene Ontology analysis for biological process for up- and downregulated genes in major sn-RNAseq clusters. Additional file 11: Table S10. Gene Ontology analysis for cellular component for up- and downregulated genes in major sn-RNAseq clusters. Additional file 12: Table S11. Number of reads for each biological replicate in bulk RNA-seq. Additional file 13: Table S12. Primer sequences for qPCR., Peer reviewed

Texto en latín grabado en la orla del escudo elíptico que corona la puerta de entrada a la capilla de San Pedro Y San Pablo desde la nave. El texto se lee en la dirección de las agujas del reloj, comienza en la cruz superior, en el escudo situado sobre la puerta de entrada a la capilla. No habla este escudo Andújar cuando trata de la Capilla de San Pedro y San Pablo. El escudo pertenecía a los León. El texto forma parte de una oración popular medieval que se encuentra en la leyenda Dorada, "De Sancto Andrea Apostolo", de Santiago de la Vorágine., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed