Resultados de investigación

(A) General gating strategy used to identify different cell subsets in lung samples. A gate based on FSC vs. SSC was followed by doublet and dead cells exclusion. From live CD45- cells, endothelial cells (CD31+, purple) and epithelial cells (EpCAM+, grey) were gated, and within epithelial cells, AT-II cells (EpCAM+ and HLA-DR+, pink) were identified. Out of live CD45+ cells and based on FSC vs. SSC, we identified a lymphocyte population in which we distinguished between non-T lymphocytes (turquoise) and T cells (dark green) based on CD3 expression; and big cells, where we identified three major subsets based on their expression of CD11b and CD11c and, subsequently, CD14 and HLA-DR markers. We identified alveolar macrophages (blue), monocytes (violet), myeloid dendritic cells (mDCs, fuchsia) and neutrophils (orange)., Peer reviewed

(A) Representative tSNE maps showing concatenated flow cytometry standard files for three different protocols based on different digestion enzymes (collagenase, liberase or trypsin) from total live cells (upper), CD45+ cells (middle) and CD45- cells (lower). (B) Bar plots showing cell-type composition (count) analyzed by flow cytometry for each tissue protocol. (C) Representative flow cytometry plots showing Surfactant Protein C (SPC) staining and its respective fluorescence minus one (FMO) control. (D) Representative flow cytometry plots showing ACE2 staining and its respective fluorescence minus one (FMO) control., Peer reviewed

General gating strategy used to evaluate the expression of inflammatory molecules in lung samples. A gate based on FSC vs. SSC was followed by doublet and dead cells exclusion. From live CD45+ cells and based on FSC vs. SSC, we identified lymphocyte population and big cells, in which we identified two subsets based on their expression of CD11b and CD14: myeloid CD11b+CD14+ cells (blue-green) and myeloid CD11b+CD14- cells (orange) are shown, Peer reviewed

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Peer reviewed

Zeolites synthesis, characterization data, and ptychography reconstruction process., Peer reviewed

The PDF file includes: • Materials and Methods • References • Supplementary Figures S1 – S7 • Supplementary Table S1, Peer reviewed

Document S1. Figures S1–S6 and Table S1. Document S2. Article plus supplemental information., Peer reviewed

3 pages. -- Supplementary tables. -- Table S1. NCBI gene accession numbers and sequences of the selected primers. -- Table S2. Additional Statistic data relative to Figure 4 Panel B. -- Table S3. Additional Statistic data relative to Figure 4 Panel B. -- Data are expressed as mean ± SD, n = 3, the significance was determined by the t-test student., Peer reviewed

Experimental procedures, protocols for the synthesis of nanoparticles, preparation of membrane electrode assemblies, cyclic voltammetry of NPs, acquisition of diffractograms, NEXAFS measurements under applied potentials, Co L- edge under reduction potentials, O K-edge under reduction and oxidation potentials, HRTEM microscopy of NPs before and after the application of a potential (oxidation or reduction)., Peer reviewed