BUSCADOR RECOLECTA

En este manual se describe el procedimiento utilizado en el muestreo de suelos de las parcelas de la Red Española de NIVELII del programa ICP-Forests. Estas parcelas forman parte de la Red de Seguimiento Intensivo (Nivel II) del Programa de Cooperación internacional para la evaluación y seguimiento de los efectos de la contaminación atmosférica en los bosques (ICP Forests). En ella se establecieron a principio de los años 90 más de 860 parcelas de observación de los ecosistemas forestales más importantes de Europa. España contribuyó con 53 parcelas de seguimiento permanente repartidas por toda su geografía. La Red iniciada en España en 1993 está conformada por 53 parcelas localizadas en ecosistemas forestales representativos de nuestro país. El objetivo del muestreo inicial (1993-96) fue la caracterización físico-química y edáfica del suelo sobre el que se desarrolla la masa forestal, como punto de partida para la monitorización de los cambios temporales en sus propiedades., Convenio ICONA-INIA para prestar apoyo científico, experimental y metodológico a la creación y seguimiento de una Red de estaciones permanentes para evaluar el estado de vigor y conservación de nuestras masas forestales, mediante la vigilancia intensiva y continua de los daños ocasionados entre otros por la contaminación atmosférica (1993-97)., Peer reviewed

Una buena fotografía representa un trozo de la Naturaleza tal como estaba en el momento de ser tomada, en este caso constituye un documento científico, que conserva indefinidamente su valor, y cuya contemplación y análisis serán siempre convenientes cualquiera que sean las interpretaciones que puedan hacerse, cuando se observe ese trozo de una realidad pasada., Fototeca Forestal Española. Ministerio de Medio Ambiente., Peer reviewed

This data set contains information on the rumen microbiome of 340 dairy cows, sequenced within the METALGEN project (RTA2015-00022-C03).-- Sequenced with a MinION from Oxford Nanopore Technology., The purpose of this data set is to serve as a training exercise to predict a complex phenotype using metagenomic data within the workshop SUPREMET 2019 "Supercomputación para la predicción de enfermedades y caracteres complejos usando información del metagenoma"., METALGEN, Ministerio de Ciencia, Innovación y Universidades, RTA2015-00022-C03., Peer reviewed

Cereales de invierno 2020., Peer reviewed

[Methodological information] Velocity probability density functions (vpdfs) and breakthrough curves (BTC) for different saturation degrees (65, 70, 80, and 100) and molecular diffusion coefficents (D) were calculated from direct numerical simulation using COMSOL multiphysics as described in the paper "Solute Trapping and the Mechanisms of Non-Fickian Transport in Partially Saturated Porous Media", With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2018-000794-S)., I.B.N, M.D. acknowledge funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. HORIZON-MSCA-2021-PF-01 (USFT). I.B.N. , J.J.H. and M.D. acknowledge the support of the Spanish Research Agency (10.13039/501100011033), Spanish Ministry of Science and Innovation through grants CEX2018-000794-S and HydroPore PID2019-106887GB-C31. J.J.H. acknowledges the support of the Spanish Research Agency (10.13039/501100011033), the Spanish Ministry of Science and Innovation and the European Social Fund ``Investing in your future'' through the ``Ram\'on y Cajal'' fellowship (RYC-2017-22300). J.J.M. gratefully acknowledges the financial support from the Swiss National Science Foundation (SNF, grant nr. 200021 178986)., Peer reviewed

The temperature-size rule hypothesized that there is a negative relationship between the size (volume) of an organism and the temperature. This applies to both unicellular and pluricellular organisms. Here, we question this hypothesis for the particular case of protozoans, because in these organisms the volume is directly related to the consumption of prey, and on most of the occasions the true volume of the cell is unknown. To prove our arguments, we designed a series of experiments with the heterotrophic dinoflagellate O. marina, including functional and numerical responses, time-dependent acclimation responses, and estimation of the protozoan volume during long periods of starvation. Our data showed that, in fact, the observed temperature-size rule in unicellular grazers results from anabolic and catabolic imbalances, and that the relationship between size and temperature weakens after proper thermal adaptation. We also showed that once prey are fully digested, the protozoan’ size is the same irrespectively of the temperature. Finally, we set the basis for proper acclimation during short-term temperature experiments, which specifies that at least 3 days should be allowed for proper temperature acclimation. We also suggest that, for trustable experiments, the grazer should be incubated at the target prey concentration for at least 24h before conducting the experiments. The ecological implications of a lack of correlation between microzooplankton size and temperature are also discussed, This research was funded by Grant PID2020-118645RB-I00 by Ministerio de Ciencia e innovación (MCIN)/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. It is a contribution of the Marine Zooplankton Ecology Group (2017 SGR 87). With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), Para Oxyrrhis marina: Tasas de ingestion a diferentes concentraciones de alimento (presa/ind/d), Tasa crecimiento (µ 1/d), Volumen (µm3); para la presa (Rhodomonas salina): volume (µm3), Peer reviewed

Methods 20 μL of plasma samples were spiked with deuterated internal standards stock solution. Then proteins were precipitated and supernatants were dried and reconstituted in methanol:water (50:50, V/V). Besides, approximately 50 mg of each tissue were placed in 2 ml tubes containing CK14 ceramic beads (Precellys). For each 50 mg of tissue, 300 μl of methanol and the deuterated internal standards were added and tissues were homogenized in a Precellys 24 Dual system equipped with a Criolys cooler (Precellys). Samples were analyzed using an Acquity UPLC system (Waters, UK) equipped with an Acquity UPLC BEH C18 column (1.7μm, 2.1 x 100 mm; Waters). The MS analysis was performed using a Waters Xevo TQ-XS mass spectrometer (Waters) with an ESI source working in the negative-ion mode., Cyclooxygenase-2 (COX-2) is involved in different liver diseases, but little is known about the significance of COX-2 or its metabolites in cholestatic injury. This study was designed to elucidate the role of COX-2 expression during the pathogenesis of cholestasis. Thus, we investigated the mechanisms underlying the role of COX-2 and its derived prostaglandins in modulating cell survival, inflammation, oxidative stress status and the synthesis and excretion of bile acids (BA) in response to cholestatic liver injury. We used genetically modified mice constitutively expressing human COX-2 (hCOX-2-Tg) specifically in hepatocytes. Transgenic mice (hCOX-2-Tg) and their wild-type (Wt) littermates were either subjected to a common bile duct ligation (BDL) to establish an experimental model of obstructive cholestasis. We performed an exhaustive analysis of the different types of bile acids (total, primary, secondary, conjugated, non-conjugated and hydrophilic, α-, β- and ω-muricholic acid) in plasma and in liver tissue from Wt and h-COX-2 Tg mice. Samples were analyzed at Instituto de Investigación Sanitaria La Fe (Valencia, Spain) detecting a total of 31 analytes., Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación 10.13039/501100011033 (PID2019-108977RB-I00), No

[EN] These project improve our understanding of the microevolutionary drivers of phenotypic plasticity, a critical process for resilience of long-lived species under climate change, and support decision making in tree genetic improvement programs and seed transfer strategies. The dataset compiles the three common garden tests (Condon, Lubrecht and Little Wolf) established in western Montana (USA) followed a Population × Family structure. Seeds were collected from 115 open-pollinated, unrelated wild mother trees in the 23 populations. Every population was represented by 5 open-pollinated families and every family was planted in all three trials. One-year-old bare-root seedlings were planted in 1974 on a 3 × 3 m spacing using a randomized complete block design at the family level with 4-tree-row plots and 5 blocks in each site. Tree growth was measured as tree height at ages 2, 4, 5, 11, 16, 21 and 27, and diameter at breast height (DBH; 1.4 m above ground) at ages 5, 11, 16, 21 and 27. DBH at age 27 was chosen as the best predictor of genetic growth potential in these trials. Means and standard errors of DBH27 were compueted at the family level within each site., The Barrié Foundation provided postdoctoral support to Raúl de Mata (EDB-CSIC). The Inland Empire Tree Improvement Cooperative (Montana, USA) collected the data and Raúl de Mata (EDB-CSIC) processed the data. This project was partially funded by a McIntire-Stennis Cooperative Forestry Research Grant MONZ-1206 from the College of Forestry and Conservation of the University of Montana., No

The efficient conservation of vulnerable ecosystems in the face of global change requires a complete understanding of how plant communities respond to various environmental factors. We aim to demonstrate that a combined use of different approaches, traits, and indices representing each of the taxonomic and functional characteristics of plant communities will give complementary information on the factors driving vegetation assembly patterns. We analyzed variation across an environmental gradient in taxonomic and functional composition, richness, and diversity of the herb-layer of a temperate beech-oak forest that was located in northern Spain. We measured species cover and four functional traits: leaf dry matter content (LDMC), specific leaf area (SLA), leaf size, and plant height. We found that light is the most limiting resource influencing herb-layer vegetation. Taxonomic changes in richness are followed by equivalent functional changes in the diversity of leaf size but by opposite responses in the richness of SLA. Each functional index is related to different environmental factors even within a single trait (particularly for LDMC and leaf size). To conclude, each characteristic of a plant community is influenced by different and even contrasting factors or processes. Combining different approaches, traits, and indices simultaneously will help us understand how plant communities work., Fundación Caja Navarra 10833 (Program “Tú Eliges, Tú Decides”). Universidad de Navarra, project “Biodiversity Data Analytics and Environmental Quality”. Universidad de Navarra, project “Red de Observatorios de la Biodiversidad de Navarra (ROBIN)”. Departamento de Educación, Gobierno de Navarra, Ayudas predoctorales para la realización de programas de doctorado de interés para Navarra; Plan de Formación y de I + D 2018 Ministerio Español de Ciencia e Innovación. Consejo Superior de Investigaciones Científicas, PIE-20223AT003. EU “NextGenerationEU/PRTR”., Peer reviewed

1. Melanins are the most common pigments in vertebrates and, as such, fufill multiple adaptive functions, including honest signaling. This is the case of male pied flycatchers Ficedula hypoleuca, whose dorsal plumage, composed of black and grey feathers pigmented by eumelanin, is sexually selected by females regarding the proportion of black. 2. However, the basis of such mating preferences and other associations with life-history traits are unknown. 3. Here I take advantage of recent advances in Raman spectroscopy analysis to investigate the monomeric composition of eumelanin, constituted by 5,6-dihydroxyindole (DHI) and 5,6-dihydroxyindole-2-carboxylic acid (DHICA) subunits, in male pied flycatchers. 4. I found that plumage blackness (percentage of dorsal black feathers) increases with the DHICA:DHI ratio of the constituent black freathers, but not with that of grey feathers. The repeatability of DHICA:DHI measurements in black feathers is two times that in grey feathers. 5. Eumelanin chemistry may thus constitute the basis of signal honesty in pied flycatchers, as females preferentially mate with males with higher relative DHICA feather contents and this may be related to the lower pro-oxidant effect of DHICA. Given the the ubiquitous nature of melanin-based pigmentation phenotypes, the monomeric composition of eumelanin should therefore be considered, instead of eumelanin as a whole, for a better understanding of how and why phenotypes are linked to life-history traits in animals., Consejo Superior de Investigaciones Científicas, Award: 202030I023., Peer reviewed