BUSCADOR RECOLECTA

(A) Schematic drawings of an S9 egg chamber illustrating the mirrorGal4 pattern of expression (mirrGal4, pink) and the point of ablation in the basal side of FCs (blue bar). NCs are in gray, BCs in yellow, FCs in purple, and BM in blue. (B) Images of life S9 control mirrGal4 and mirr>AbiRNAi egg chambers expressing Resille-GFP before and after FCs bonds are ablated. Blue bars indicate points of ablation. (C) Quantification of initial velocity of vertex displacement of the indicated ablated bonds. (D) Schematic drawing of an S9 egg chamber illustrating the mirrGal4 pattern of expression (pink) and the point of ablation in the NCs (blue bar). (E) Images of life S9 egg chambers of the indicated genotypes before and after NC bonds are ablated. Blue bars indicate points of ablation. (F) Quantification of initial velocity of vertex displacement of the indicated ablated bonds. (G–H’) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. Discontinuous yellow lines demarcate the region between the anterior border of the egg chamber and the oocyte anterior membrane. (I, J) Quantification of BC migration speed in the area anterior to (I) and at the (J) mirr expressing region. The statistical significance of differences was assessed with a t test, * P value < 0.05, ** P value < 0.01, and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bar in B, E, and G–H’, 5 μm, 10 μm, and 20 μm, respectively. The raw data underlying panels C, F, I, and J are available in S1 Data. BC, border cell; BM, basement membrane; FC, follicle cell; GFP, green fluorescent protein; NC, nurse cell., Peer reviewed

[Descripción de los métodos utilizados para la recopilación/generación de datos] La evaluación de La Noche de l@s Investigador@s por parte de sus protagonistas se ha realizado a partir de tres cuestionarios online habilitados en la web nocheinvestiga.es adaptados a tres perfiles diferentes: 1) investigadores/as, 2) asistentes y 3) menores de 18 años. La aplicación de encuestas online ha sido desarrollada íntegramente por IESA CSIC sin apoyo de ningún software comercial, garantizando de este modo la privacidad, seguridad y calidad de los datos. El trabajo de campo se realizó entre el 23 de noviembre y el 20 de diciembre de 2020. La crisis sobrevenida ha implicado cambios y adaptaciones importantes. La realización online de la práctica totalidad de las actividades ha determinado cambios en la metodología de evaluación prevista, adaptándola a esta situación condicionante. Así, la evaluación dirigida a los asistentes se ha realizado totalmente online, en parte cumplimentando el cuestionario tras la realización de la actividad y en parte tras invitación dirigida al correo electrónico suministrado por el participante en el momento de inscribirse en la misma, en las actividades que así lo requerían. La evaluación dirigida a investigador@s y organizador@s se ha realizado igualmente online, tras invitación mediante correo electrónico. Por otro lado, en esta edición se ha optado por administrar un cuestionario específico a los y las participantes menores de 18 años, con preguntas orientadas a detectar sus potenciales vocaciones científicas. Por lo tanto, se ha dispuesto de tres cuestionarios diferentes. La metodología empleada en la evaluación de La Noche de l@s investigador@s ha permitido recabar casi 1.225 cuestionarios que se distribuyen por perfiles del siguiente modo: • Un cuestionario destinado a l@s participantes en las actividades mayores de 17 años, administrado y cumplimentado de manera online tras la realización de las actividades respectivas (acceso directo) o tras una invitación vía email. En total se ha cumplimentado el cuestionario por 475 personas, 330 en acceso directo y 145 por email tras invitación. • Un cuestionario dirigido a las personas que han realizado las actividades (investigador@s, técnic@s, voluntari@s, ...), cumplimentado igualmente de manera online. En concreto este cuestionario ha sido cumplimentado por 554 “investigadores”. • Un cuestionario destinado a participantes menores de 18 años, con preguntas orientadas a captar su potencial vocación científica, y administrado también directamente tras la realización de la actividad y tras invitación por email. En total se han cumplimentado 196 cuestionarios a menores., [Modalidades de tratamiento de los datos] La grabación de los datos fue automática y la codificación de preguntas abiertas se realizó manualmente. En la depuración de los datos se han eliminado los casos que no habían cumplimentado el 75% del cuestionario., El libro de códigos está disponible en español y en traducción al inglés., [ES] La Noche Europea de los Investigadores es un evento de divulgación científica de alcance europeo que muestra de forma divertida e inspiradora la diversidad de la ciencia y su repercusión en la vida cotidiana de los ciudadanos. El evento es una iniciativa de la Comisión Europea y se financia a través de las Acciones Marie Sklodowska Curie (MSCA) del Programa Marco de Investigación e Innovación de la UE. La novena edición se celebró en 371 ciudades europeas, entre ellas las ocho capitales andaluzas el 27 de noviembre de 2020. Esta edición de La Noche en Andalucía ha sido mayoritariamente online, debido igualmente a la crisis sanitaria, de modo que se ha podido disfrutar directamente desde la web. Han sido más de 650 actividades para acercar la ciencia y a las personas que investigan al público en general, llevadas a cabo por casi 3.000 investigadores. Este informe presenta datos significativos para medir el impacto de la Noche Europea de los Investigadores en Andalucía, así como la evaluación realizada por sus participantes: asistentes e investigadores., [EN] The European Researchers' Night is a Europe-wide scientific dissemination event that displays the diversity of science and its impact on citizens' daily lives in fun, inspiring ways. The event is an initiative of the European Commission and is funded through the Marie Sklodowska Curie Actions (MSCA) of the EU’s Framework-Programme for Research and Innovation. The ninth edition was held in 371 European cities, including the eight Andalusian capitals on November 27, 2020. This edition of La Noche en Andalucía was mostly online, also due to the health crisis, so it could be enjoyed directly from the web. There have been more than 650 activities to bring science and researchers closer to the general public, carried out by almost 3,000 researchers. This report presents significant data to measure the impact of the European Researchers' Night in Andalusia, as well as the evaluation conducted by its participants: attendees and researchers., Project funded by the European Commission’s Horizon Europe Research Framework Programme, in the framework of Marie Sktodowska-Curie actions., 1. Lista de archivos: - THE NIGHT 2020 Readme_EN.pdf - THE NIGHT 2020 Readme_SP.pdf A) Archivos para el dataset de Asistentes: - THE NIGHT 2020 (Attendees)_Data file.csv - THE NIGHT 2020 (Attendees)_Data file.sav - THE NIGHT 2020 (Attendees)_Codebook_EN.pdf - THE NIGHT 2020 (Attendees)_Codebook_SP.pdf - THE NIGHT 2020 (Attendees)_Questionnaire.pdf B) Archivos para el dataset de Investigadores - THE NIGHT 2020 (Researchers)_Data file.csv - THE NIGHT 2020 (Researchers)_Data file.sav - THE NIGHT 2020 (Researchers)_Codebook_EN.pdf - THE NIGHT 2020 (Researchers)_Codebook_SP.pdf - THE NIGHT 2020 (Researchers)_Questionnaire.pdf C) Archivos para el dataset de Asistentes jóvenes: - THE NIGHT 2020 (Young attendees)_Data file.csv - THE NIGHT 2020 (Young attendees)_Data file.sav - THE NIGHT 2020 (Young attendees)_Codebook_EN.pdf - THE NIGHT 2020 (Young attendees)_Codebook_SP.pdf - THE NIGHT 2020 (Young attendees)_Questionnaire.pdf, Peer reviewed

(A–A”) Schematic drawings of an S9 egg chamber illustrating the point of ablation (blue bar) on the basal (A’) and apical (A”) side of a FC. NCs are in gray, BCs in yellow, FCs in purple, and BM in blue. (B, E, H, K) Images of the basal (B, E) and apical (H, K) sides of life S9 egg chambers of the indicated genotypes before and after FC bonds are ablated. Blue bars indicate points of ablation. (C, F, I, L) Quantification over time of initial velocity of vertex displacement and (D, G, J, M) vertex displacement of the indicated ablated bonds. The statistical significance of differences was assessed with a t test, * P value < 0.05, ** P value < 0.01, and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in B, E, H, and K, 5 μm. The raw data underlying panels C, D, F, G, I, J, L, and M are available in S1 Data. BC, border cell; BM, basement membrane; FC, follicle cell; NC, nurse cell., Peer reviewed

[Description of methods used for collection/generation of data] Individual capture hsitories were built from long-term individual capture-recapture data of adult Scopoli’s shearwater Calonectris diomedea breeding in Pantaleu islet. Some of these individuals were also equipped with geoloctaors, that when recovered may provide information of individual wintering areas. All the information about the deployment of geolocators devices and wintering areas visited are encoded in the individual capture histories.The file inlcudes 23 annual encounter occasions, which translate into 45 coded encounter occasions, with the ‘inter’ encounter occasions encoding information about presence in the colony and migration decisions, and the ‘intra’ occasions encoding information about whether a geolocator had been deployed or not at the end of the season., No utilizar sin el previo consentimiento de los propietarios., Este fichero contiene información de las historias de captura individuales de Pardela cenicienta criando en el islote de Pantaleu, Mallorca, durante el período 2000-2022. En estas historias de captura anuales se ha incluido información de la colocación de dispositivos geolocalizadores en algunos de estos individuos, y la información que se obtuvo de las zonas de invernada visitadas por estos individuos., Peer reviewed

(A) Schematic drawings of an S9 egg chamber illustrating the point of ablation (blue bar). NCs are in gray, BCs in yellow, FCs in purple, and BM in blue. (B) Images of life S9 control (tjGal4) and tj>LanB1RNAi egg chambers expressing Resille-GFP before and after NC bonds are ablated. Blue bars indicate points of ablation. (C) Quantification of initial velocity of vertex displacement and (D) vertex displacement over time of the indicated ablated bonds. (E) Schematic drawing of an S9 egg chamber, similar to the one shown in A, illustrating the mirrorGal4 pattern of expression (mirrGal4, pink). (F, I) Life images of NCs located in the area anterior to (F) and at the (I) the mirr expressing region of S9 control (mirrGal4) and mirr>EHBP1mCh egg chambers expressing Resille-GFP before and after NC bonds are ablated. Blue bars indicate points of ablation. (G, J) Quantification over time of initial velocity of vertex displacement and (H, K) vertex displacement over time of the indicated ablated bonds. The statistical significance of differences was assessed with a t test, * P value < 0.05 and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in B, F, and I, 10 μm. The raw data underlying panels C, D, G, H, J, and K are available in S1 Data. BC, border cell; BM, basement membrane; FC, follicle cell; GFP, green fluorescent protein; NC, nurse cell., Peer reviewed

(A–D) Stills taken from live imaging of BCs from egg chambers of the designated genotypes. The blue circles indicate the BC cluster bodies. (E, F) Mean number of protrusions observed per frame (snapshots) in each direction from BC clusters of egg chambers of the indicated genotypes. (G–J’) Stills taken from live imaging of BC clusters from egg chambers of the designated genotypes. The pink asterisks mark the BC being followed. White arrows indicate the direction of the movement. (K–M) Quantification of BC cluster rotation. The statistical significance of differences was assessed with a t test, * P value < 0.05. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A–D and G–J, 10 μm. The raw data underlying panels E, F and K–M are available in S1 Data. BC, border cell; BM, basement membrane., Peer reviewed

(A–B”) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. (C, D) Quantification of BC migration speed in egg chambers of the indicated genotypes in the area anterior to (C) and at the (D) mirr expressing region. (E) Quantification of egg chambers with complete BC migration. The statistical significance of differences was assessed with a t test, * P value < 0.05. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A” and B”, 20 μm. The raw data underlying panels C–E are available in S1 Data. BC, border cell; FC, follicle cell., Peer reviewed

(A) Position of ovaries within a Drosophila female (left) and schematic of Drosophila ovaries showing an ovariole and its egg chambers (right). BC, border cells; Oo, oocyte; NCs, nurse cells; FCs, follicle cells; StFCs, stretch FCs; BM, basement membrane. (B) Diagram of an ovariole showing stages 8, 9, and 10 egg chambers (S8–S10). Anterior is left, posterior right. (C) Three-dimensional diagram of a S9 egg chamber showing the migration of the BCs between the NCs. (D–G’) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. Discontinuous yellow lines demarcate the region between the anterior border of the egg chamber and the oocyte anterior membrane. (H–J) Quantification of BC migration speed during the whole migratory process (H) and during the early (I) and late (J) phases in egg chambers of the indicated genotypes. The statistical significance of differences was assessed with a t test, ** P value < 0.01. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A’, B’, C’ and D’, 20 μm. The raw data underlying panels H–J are available in S1 Data., Peer reviewed

Suppl. Figure 1. Percentage of FES patients in symptomatic remission throughout the study., Suppl. Figure 2. Percentage of FES patients in functional remission throughout the study., Suppl. Figure 3. Clustered boxplot displaying the mean depression scores per time point (measured using the Hamilton Depression Rating Scale) in the subgroup meeting criteria for a depressive episode at baseline (assessed through the SCID-I) versus the subgroup without a depressive episode at baseline., Suppl. Table 1. Baseline demographics and clinical characteristics of study completers and drop-outs., Suppl. Table 2. Number of patients in symptomatic and functional remission throughout the period of follow-up., Suppl. Table 3. Generalized linear mixed models: pairwise contrasts for symptomatic and functional remission., Suppl. Table 4. Linear mixed models: estimated marginal means for PANSS, GAF, SOFAS, CGI, GF-S, GF-R and HAM-D., Suppl. Table 5. Linear mixed models: estimates of fixed effect time on PANSS, GAF, SOFAS, CGI, GF-S, GF-R and HAM-D., Suppl. Table 6. Number of patients admitted to the hospital since the previous visit due to psychotic symptoms and due to psychiatric reasons in general., Suppl. Table 7. Substances used at least once in the past three months (WHO-ASSIST)., Suppl. Table 8. Number of patients reporting daily or almost daily substance use in the past three months, separated per time point and substance category (WHO-ASSIST)., Suppl. Table 9. Number of patients reporting daily or almost daily substance use in the past three months, separated for the group of symptomatic remitters and non-remitters at month 12., Suppl. Table 10. Baseline demographics and clinical characteristics of symptomatic remitters and non-remitters at month 12., Suppl. Table 11. Summary of sociodemographics and baseline clinical characteristics of FES participants, separated per country., Suppl. Table 12. Number of FES patients in symptomatic remission throughout the period of follow-up, separated per country., Suppl. Table 13. Number of FES patients in functional remission throughout the period of follow-up, separated per country., Suppl. Table 14. Number of FES patients admitted to the hospital for psychiatric reasons throughout the period of follow-up, separated per country., Suppl. Table 15. Number of FES patients admitted to the hospital for psychosis throughout the period of follow-up, separated per country., Suppl. Table 16. Number of FES patients using antipsychotic medication throughout the period of follow-up, separated per country., Suppl. Table 17. Overview of subjects not meeting eligibility criteria., Suppl. Table 18. Number of patients in symptomatic and functional remission throughout the period of follow-up when using alternative definitions., Suppl. Table 19. Generalized linear mixed models: pairwise contrasts for symptomatic and functional remission when using alternative definitions., Peer reviewed

The basement membrane (BM) is a specialized extracellular matrix (ECM), which underlies or encases developing tissues. Mechanical properties of encasing BMs have been shown to profoundly influence the shaping of associated tissues. Here, we use the migration of the border cells (BCs) of the Drosophila egg chamber to unravel a new role of encasing BMs in cell migration. BCs move between a group of cells, the nurse cells (NCs), that are enclosed by a monolayer of follicle cells (FCs), which is, in turn, surrounded by a BM, the follicle BM. We show that increasing or reducing the stiffness of the follicle BM, by altering laminins or type IV collagen levels, conversely affects BC migration speed and alters migration mode and dynamics. Follicle BM stiffness also controls pairwise NC and FC cortical tension. We propose that constraints imposed by the follicle BM influence NC and FC cortical tension, which, in turn, regulate BC migration. Encasing BMs emerge as key players in the regulation of collective cell migration during morphogenesis., Peer reviewed