Resultados de investigación

NMR datasets used in https://doi.org/10.1021/acs.analchem.8b01196. In the corresponding study, we have performed a comparative chemometric analysis between untargeted 1H NMR and 1H-13C HSQC NMR analyses of metabolomics samples from Saccharomyces cerevisiae (yeast) extracts. Specifically, yeast was grown in two different liquid media and their metabolism was characterized at 8 different time-points of a 3-day period. The two media used, YPD (Yeast Peptone Dextrose) and YSC (Yeast nitrogen base Synthetic Complete), are broadly used in yeast lab routines, and results from this analysis should be of interest for improving lab methodologies involving yeast., Peer reviewed

The exposure protocol involved zebrafish embryos exposed in groups of 20 to various concentrations of chemical compounds, with five replicates per treatment. The concentrations ranged from the lowest observed effect concentrations (LOECs) to control levels. After exposure, embryos were collected, washed, frozen, and stored. Metabolites were extracted from individual embryo pools using methanol and methionine sulfone. The extraction process included vortexing, sonication, and centrifugation, followed by addition of water and chloroform. The aqueous fraction was dried and reconstituted using acetonitrile-water solution. Liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) was used for analysis. Chromatographic separations were carried out on a hydrophilic interaction liquid chromatography (HILIC) column. Mass spectrometry was performed using an Orbitrap mass spectrometer with electrospray ionization in positive and negative modes. The mass spectra were acquired at high resolution, and fragmentation scans were used for metabolite identification. The overall process aimed to analyze the metabolomic profile of zebrafish embryos exposed to different chemical concentrations., Peer reviewed

The dataset includes two Excel spreadsheets with the soil and water characteristics from samples obtained during wet (April) and dry (September) seasons in 2019, and at the end of the experiment, July 2020. The sampling was designed to characterize the soil and water in the context of the study of soil redox conditions using IRIS (Indicators of Reducing of Soils) devices. The selected sampling locations represent a gradient of water within the playa-lake: bare soil very frequently flooded (SAL-1); bare soil often flooded (SAL-2); and the plot colonized by annual and perennial halophytes (SAL-3). Soil data includes the main description of the soil layers per date in the three sampling plots, and their chemical and physical analytical data determined in the field and in the lab. We analyzed water from three different origins: i) surface water when present, ii) shallow groundwater from piezometers up to 80 cm depth, iii) suction probes installed at 20 and 40 cm depth, and iv) saturated paste extracts of soil samples. Photograph P1170434.jpg shows a view of the field setting of the experiment in 2019, April 17., The data corresponds to the Spanish study area, the Salineta inland saline wetland (41°28'55.34"N, 0° 9'23.59"W) located 60 km far from Zaragoza city, in one of the most arid areas of the Central Ebro Basin, NE Spain. In this project we studied the redox conditions of the hypersaline soils in Salineta playa-lake, which are subjected to intermittent flooding, along a period of 17 months, in 2019 and 2020., These data belong to the research project AQUASALT, EraNET that in Spain has been supported by grant PCI2018-09299 funded by MCIN/AEI/10.13039/50110 0 011033 and co-funded by the European Union., Peer reviewed

[Introduction]: Secreted mucins are highly O-glycosylated glycoproteins produced by goblet cells in mucosal epithelia. They constitute the protective viscous gel layer overlying the epithelia and are involved in pathogen recognition, adhesion and expulsion. The gill polyopisthocotylidan ectoparasite Sparicotyle chrysophrii, feeds on gilthead seabream (Sparus aurata) blood eliciting severe anemia., [Methods]: Control unexposed and recipient (R) gill samples of gilthead seabream experimentally infected with S. chrysophrii were obtained at six consecutive times (0, 11, 20, 32, 41, and 61 days post-exposure (dpe)). In histological samples, goblet cell numbers and their intensity of lectin labelling was registered. Expression of nine mucin genes (muc2, muc2a, muc2b, muc5a/c, muc4, muc13, muc18, muc19, imuc) and three regulatory factors involved in goblet cell differentiation (hes1, elf3, agr2) was studied by qPCR. In addition, differential expression of glycosyltransferases and glycosidases was analyzed in silico from previously obtained RNAseq datasets of S. chrysophrii-infected gilthead seabream gills with two different infection intensities., [Results and Discussion]: Increased goblet cell differentiation (up-regulated elf3 and agr2) leading to neutral goblet cell hyperplasia on gill lamellae of R fish gills was found from 32 dpe on, when adult parasite stages were first detected. At this time point, acute increased expression of both secreted (muc2a, muc2b, muc5a/c) and membrane-bound mucins (imuc, muc4, muc18) occurred in R gills. Mucins did not acidify during the course of infection, but their glycosylation pattern varied towards more complex glycoconjugates with sialylated, fucosylated and branched structures, according to lectin labelling and the shift of glycosyltransferase expression patterns. Gilthead seabream gill mucosal response against S. chrysophrii involved neutral mucus hypersecretion, which could contribute to worm expulsion and facilitate gas exchange to counterbalance parasite-induced hypoxia. Stress induced by the sparicotylosis condition seems to lead to changes in glycosylation characteristic of more structurally complex mucins., Peer reviewed

[Introduction]: Secreted mucins are highly O-glycosylated glycoproteins produced by goblet cells in mucosal epithelia. They constitute the protective viscous gel layer overlying the epithelia and are involved in pathogen recognition, adhesion and expulsion. The gill polyopisthocotylidan ectoparasite Sparicotyle chrysophrii, feeds on gilthead seabream (Sparus aurata) blood eliciting severe anemia., [Methods]: Control unexposed and recipient (R) gill samples of gilthead seabream experimentally infected with S. chrysophrii were obtained at six consecutive times (0, 11, 20, 32, 41, and 61 days post-exposure (dpe)). In histological samples, goblet cell numbers and their intensity of lectin labelling was registered. Expression of nine mucin genes (muc2, muc2a, muc2b, muc5a/c, muc4, muc13, muc18, muc19, imuc) and three regulatory factors involved in goblet cell differentiation (hes1, elf3, agr2) was studied by qPCR. In addition, differential expression of glycosyltransferases and glycosidases was analyzed in silico from previously obtained RNAseq datasets of S. chrysophrii-infected gilthead seabream gills with two different infection intensities., [Results and Discussion]: Increased goblet cell differentiation (up-regulated elf3 and agr2) leading to neutral goblet cell hyperplasia on gill lamellae of R fish gills was found from 32 dpe on, when adult parasite stages were first detected. At this time point, acute increased expression of both secreted (muc2a, muc2b, muc5a/c) and membrane-bound mucins (imuc, muc4, muc18) occurred in R gills. Mucins did not acidify during the course of infection, but their glycosylation pattern varied towards more complex glycoconjugates with sialylated, fucosylated and branched structures, according to lectin labelling and the shift of glycosyltransferase expression patterns. Gilthead seabream gill mucosal response against S. chrysophrii involved neutral mucus hypersecretion, which could contribute to worm expulsion and facilitate gas exchange to counterbalance parasite-induced hypoxia. Stress induced by the sparicotylosis condition seems to lead to changes in glycosylation characteristic of more structurally complex mucins., Peer reviewed

(A) CBK1 cbk1Δ cdc15-2 (YMF3869), (B) cbk1-6E cbk1Δ cdc15-2 (YMF3763), (C) cbk1-5E-E409S cbk1Δ cdc15-2 (YMF4279), (D) cbk1-5E-E574T cbk1Δ cdc15-2 (YMF4280), and (E) cbk1-9A cbk1Δ cdc15-2 (YMF3764) cells were grown in YPD and arrested in late anaphase by raising the temperature to 37 °C before rapamycin was added to half of the culture for 20 min. Subsequently, to allow progression through the cell cycle, cells were released in the absence (i) or presence (ii) of rapamycin. Samples were taken at the indicated times to study cell-cycle progression by flow cytometry. Schematic illustration of cbk1-9A mutant in which phosphosites containing serines or threonines followed by prolines were changed to alanine to block phosphorylations (iii). FACS graphs can be found in the supplementary FACS file (S1 File)., pbio.3002263.s006.pdf, Peer reviewed

(A) DSE4-6HA cdc15-2 (YMF4029) cells were grown in YPD and arrested in late anaphase by shifting the temperature to 37 °C before the addition of DMSO (−) or rapamycin (+) for 20 min. Subsequently, cells were released from the anaphase arrest in the absence (−) or presence (+) of rapamycin before protein extracts were prepared from shown time points and analysed by immunoblotting. Raw data for blot can be found in Supporting information (S6A Raw Images). (B) iHA-CTS1 cdc14-1 cells (YMF4088) were grown and processed as in A. Protein extracts were prepared from indicated time points and analysed by immunoblotting. Raw data for blot can be found in Supporting information (S6B Raw Images). (C) CTS1-GFPEnvy cdc14-1 cells (YMF4231) were grown as in C. Samples were taken at the indicated times to determine the proportion of cells with Cts1 at the division site in the presence (i) of rapamycin. Examples of cells are shown for the 60 min time point after the release at 24 °C in the presence (ii) of rapamycin. Scale bar indicates 5 μm. Underlying data for all the graphs can be found in S1 Data file., pbio.3002263.s007.pdf, Peer reviewed

Supporting_Information - updated – Supporting_Information.pdf – Table S1.xlsx – Table S2.xlsx – Table S3.xlsx, Peer reviewed

[Motivation]: The propensity of plant tissues to burn (i.e. their flammability) is a keytrait to understand fire regimes in many ecosystems across the globe. Measuring plantflammability under laboratory conditions allows us to improve both our understand-ing of plant evolutionary processes and modelling tools for simulating fire hazard andbehaviour. Plant flammability has been studied from different but complementary dis-ciplines (e.g. physics, chemistry, ecology, evolution, forestry). However, information isscattered and standardized terminology is lacking, which slows down the progress ofresearch on plant flammability. Here we provide an open access global database onplant flammability traits measured under laboratory conditions aiming to: (a) identifythe diversity of methodologies to measure plant flammability under laboratory condi-tions; (b) standardize the associated terminology; and (c) find geographical, ecological,and taxonomic gaps in our knowledge on plant flammability. We hope this databasewill stimulate transdisciplinary research and provide useful information to better copewith an increasingly flammable planet., [Main Types of Variables Contained]: The FLAMITS database contains 19,972 records of 40 flammability variables (classified according to the measured component of flammability). For each record, relevant details of the flammability experiment are given, such as the burning device, the ignition source, and the burnt plant part. In addition, FLAMITS compiles taxonomic and functional data of the studied species and information on the study site (i.e. locality, geographic coordinates, biome, biogeographic realm, and fire activity)., [Spatial Location and Grain]: We compiled data from 295 studies in 39 countries and distributed across 12 biomes worldwide., [Time Period and Grain]: The last 62.5 years (1961 to 15th May 2023)., [Major Taxa and Level of Measurement]: 1790 plant taxa from 186 families, 883 genera, and 1784 species., [Software Format]: Five text files (.csv), relationally linked., Agencia Nacional de Investigación yDesarrollo, Grant/Award Number:PIA/BASAL FB210006 and 21190817; Dirección de Investigación, Universidad Austral de Chile, Grant/Award Number:TD-2021- 01; Fondo Nacional de Desarrollo Científico y Tecnológico, Grant/Award Number: 1190999; Generalitat Valenciana, Grant/AwardNumber: Promteo/2021/040, Peer reviewed

1 -xlsx File (19.0 kB). Under Commons Attribution 4.0 International., Field data from four Mediterranean shrublands monitored during an extreme drought in 2014 (hydrological year) in SE Spain. Data are plant dieback of the total plant community and main plant biotypes (shrubs, subshrubs and grasses), vegetation structure attributes (phytovolume, plant height, LAI, and sizes of patch and inter-patch areas), soil properties (soil depth and soil surface cover types) and water availability indicators (soil moisture and environmental variables)., Peer reviewed