Resultados totales (Incluyendo duplicados): 46350
Encontrada(s) 4635 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361338
Dataset. 2023

THE DOMINANT-NEGATIVE ACTIVITY OF TELEOST AQP1AB1 AND -1AB2 SPLICE FORMS [DATASET]

  • Ferré, Alba
  • Chauvigné, François
  • Zapater, Cinta
  • Finn, Roderick N.
  • Cerdà, Joan
(A-C) Changes in Pf of X. laevis oocytes injected with water or expressing SaAqp1ab1-WT (A), HhAqp1ab1-WT (B) or SsAqp1ab2-WT (C) alone or in combination with different amounts of the isoforms of each paralog. For SsAqp1ab2-WT and splice forms, oocytes were co-injected with halibut YwhazLb and exposed to FSK. (D) Effect of the inhibition of the oocyte Pf by the different Aqp1ab1 splice forms, at a concentrations that produced approximately half-maximal reduction (as observed in A-C), in SaAqp1ab1 or HhAqp1ab1 expressing oocytes in the presence or absence of YwhazLa, and exposed to FSK. The percentage of Pf inhibition elicited by each isoform under the two conditions is indicated in each plot. In all panels, data are the mean ± SEM (n indicated above each bar) and were statistically analyzed by an unpaired Student’s t-test (*, P < 0.05: **, P < 0.01; ***, P < 0.001; with respect to oocytes injected with the WT form alone)., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361338
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361338
HANDLE: http://hdl.handle.net/10261/361338
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361338
PMID: http://hdl.handle.net/10261/361338
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361338
Ver en: http://hdl.handle.net/10261/361338
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361338

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361340
Dataset. 2018

SUPPLEMENTARY MATERIALS: 3D FRACTALS AS SERS ACTIVE PLATFORMS: PREPARATION AND EVALUATION FOR GAS PHASE DETECTION OF G-NERVE AGENTS

  • Lafuente, Marta
  • Berenschot, Erwin J. W.
  • Tiggelaar, Roald M.
  • Mallada, Reyes
  • Tas, Niels R.
  • Pina, María Pilar
Figure S1: UV-VIS spectra of the SERS substrates herein studied: Glass_ Ag, Glass_AuNPs, Glass_Ag_AuNPs, 1G_Ag_AuNPs, 3G_Ag_AuNPs; Figure S2: SERS spectra of 6 spots recorded on bottom and top of 1G_Ag_AuNPs sample upon exposure to 1.2 ppmV DMMP in gas phase., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361340
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361340
HANDLE: http://hdl.handle.net/10261/361340
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361340
PMID: http://hdl.handle.net/10261/361340
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361340
Ver en: http://hdl.handle.net/10261/361340
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361340

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361359
Dataset. 2013

SUPPORTING INFORMATION: ABERRATION-CORRECTED STEM ANALYSIS OF A CUBIC CD ARRAY ENCAPSULATED IN ZEOLITE A

  • Mayoral, Álvaro
  • Readman, Jennifer E.
  • Anderson, P. A.
Additional proposed models and more STEM images along different crystallographic orientations together with the simulated data are included., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361359
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361359
HANDLE: http://hdl.handle.net/10261/361359
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361359
PMID: http://hdl.handle.net/10261/361359
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361359
Ver en: http://hdl.handle.net/10261/361359
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361359

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361367
Dataset. 2023

TELEOST AQP1AB1 AND -1AB2 SPLICE FORMS GENERATE DISTINCT MECHANISMS OF DOMINANT-NEGATIVE REPRESSION [DATASET]

  • Ferré, Alba
  • Chauvigné, François
  • Zapater, Cinta
  • Finn, Roderick N.
  • Cerdà, Joan
(A-C) Double immunolocalization of SaAqp1ab1-WT, Flag-tagged HhAqp1ab1-WT or Flag-tagged SsAqp1ab2-WT (green) with the respective HA-tagged splice forms in X. laevis oocytes as indicated. Oocytes expressing SsAqp1ab2-WT and splice forms were co-injected with halibut YwhazLb and exposed to FSK. In all panels, the plasma membrane is indicated by an arrowhead, whereas the co-localization of WT aquaporins and splice forms is indicated by arrows. Scale bars, 10 μm. (D-F) Representative immunoblots of total (TM) and plasma (PM) membrane protein extracts from oocytes injected with water (W) or co-expressing the different aquaporin WT channels with increasing amounts of the splice forms as indicated. (G-I) Illustrative immunoblots of precipitated proteins from oocytes treated as in A-C using anti-SaAqp1ab1 or anti-Flag (for HhAqp1ab1 and SsAqp1ab2) antibodies, and revealed with antibodies against SaAqp1ab1, Flag or HA. In D-I, upper blots were probed with SaAqp1ab1 (D and G) or Flag (E, F, H and I) antibodies, whereas in all panels the lower blots were probed with HA antibodies. Molecular mass markers (kDa) are on the left. The arrowhead in F and I indicate potential post-translational modifications of the SsAqp1ab1_v2 isoform., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361367
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361367
HANDLE: http://hdl.handle.net/10261/361367
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361367
PMID: http://hdl.handle.net/10261/361367
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361367
Ver en: http://hdl.handle.net/10261/361367
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361367

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361372
Dataset. 2020

SUPPLEMENTARY MATERIAL BIOCONVERSION OF FISH DISCARDS THROUGH THE PRODUCTION OF LACTIC ACID BACTERIA AND METABOLITES: SUSTAINABLE APPLICATION OF FISH PEPTONES IN NUTRITIVE FERMENTATION MEDIA

  • Vázquez, José Antonio
  • Durán, Ana
  • Menduiña, Araceli
  • Nogueira, Margarita
  • Gomes, Ana
  • Antunes, Joana
  • Freitas, Ana Cristina
  • Dagá, Esther
  • Dagá, Paula
  • Valcárcel Barros, Jesús
1 file, Supplementary material for the article https://doi.org/10.3390/foods9091239, Figure S1: Culture kinetics of Lb 1.-- Figure S2: Culture kinetics of Ln.-- Figure S3: Culture kinetics of Lb 3.-- Figure S4: Economical evaluation of Lb 2 bioproduction costs.-- Figure S5: Economical evaluation of Ln bioproduction costs.-- Figure S6: Economical evaluation of Lb 3 bioproduction costs.-- Table S1: Parameter kinetics of Lb 2 cultures.-- Table S2: Continuation of Table S2.-- Table S3: Parameter kinetics of Ln cultures.-- Table S4: Continuation of Table S3.-- Table S5: Parameter kinetics of Lb 3 cultures.-- Table S6: Continuation of Table S5.-- Table S7: Ranges of productive yields.-- Table S8: Amino acids content of fish discard peptones.-- Table S9: Continuation of Table S8, Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361372
HANDLE: http://hdl.handle.net/10261/361372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361372
PMID: http://hdl.handle.net/10261/361372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361372
Ver en: http://hdl.handle.net/10261/361372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361372

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361374
Dataset. 2023

PRODUCTION OF SENEGALESE SOLE TOTAL AQP1AB2 AND AQP1AB2_V1 SPECIFIC ANTISERA AND IMMUNOBLOT ANALYSIS OF AQP1AB2 SPLICE VARIANTS PROTEIN EXPRESSION IN DEVELOPING OVARIAN FOLLICLES [DATASET]

  • Ferré, Alba
  • Chauvigné, François
  • Zapater, Cinta
  • Finn, Roderick N.
  • Cerdà, Joan
(A) Amino acid sequence alignment of SsAqp1ab2-WT, SsAqp1ab2_v1 and SsAqp1ab2_v2 splice forms highlighting the sequences employed for the production of the sole-specific Aqp1ab2 and Aqp1ab2_v1 antibodies (α-Aqp1ab2-Nt and α-Aqp1ab2_v1, in red and blue color, respectively). The N- and C-termini of the WT channel, the six transmembrane helices (TM1-TM6) and the two conserved NPA motifs are also indicated. (B) Immunoblots of X. laevis oocytes injected with water or expressing Aqp1ab2-WT, SsAqp1ab2_v1 or SsAqp1ab2_v2 and probed with the α-Aqp1ab2-Nt or α-Aqp1ab2_v1 antisera separately. (C) Representative photomicrographs of sole ovarian follicles at different developmental stages during oocyte growth and maturation. PG, primary growth stage; CA, cortical alveoli stage. Scale bars, 50 and 250 μm. (D) Immunoblots of Aqp1ab2-WT, Aqp1ab2_v1 and Aqp1ab2_v2 in protein extracts from previtellogenic (Pv), vitellogenic (Vt), hydrating (H) and mature (Mt) follicle-enclosed oocytes, as well as from ovulated oocytes (Ov), using the generated antisera. Alpha tubulin (Tuba) was used as loading control. Duplicated blots (right) were run in parallel and incubated with the primary antibodies preadsorbed by the antigenic peptide to test for specificity. The brackets indicate potential post-translational modifications. (E) Hoechst staining of intact vitellogenic follicles and defolliculated oocytes. Scale bar, 100 μm. (F) Immunoblots of Aqp1ab2 isoforms in total protein extracts from intact or defolliculated vitellogenic follicles (+/- follicle cells, Fc). In B, D and E, the arrowheads indicate aquaporin monomers, whereas the asterisks in D and E indicate cross-reactive polypeptides revealed with the Aqp1ab2_v1 antiserum. In all blots, molecular mass markers (kDa) are on the left., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361374
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361374
HANDLE: http://hdl.handle.net/10261/361374
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361374
PMID: http://hdl.handle.net/10261/361374
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361374
Ver en: http://hdl.handle.net/10261/361374
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361374

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361381
Dataset. 2023

IMMUNOLOCALIZATION OF TOTAL AQP1AB2 AND THE AQP1AB2_V1 ISOFORM IN DEVELOPING SENEGALESE SOLE OVARIAN FOLLICLES [DATASET]

  • Ferré, Alba
  • Chauvigné, François
  • Zapater, Cinta
(A, D, G. J) Representative histological sections of follicle-enclosed oocytes at previtellogenesis, including the primary growth (A) and cortical alveoli (D) stages, vitellogenic (G) and maturing and hydrating (J) stage stained with hematoxylin and eosin. (B-C, E-F, H-I, K-L) Bright field (BF) images and immunostaining of total Aqp1ab2 or Aqp1ab2_v1 (red) in follicles in each of the different stages using the sole-specific α-Aqp1ab2-Nt and α-Aqp1ab2_v1 antisera. Sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue) and wheat germ agglutinin (WGA; green). Control sections incubated with preabsorbed antisera were negative (S1 Fig). The arrows and arrowheads, indicate granulosa and theca cells, respectively, surrounding the oocyte. Abbreviations: o, oocyte; y, yolk globule; gv, germinal vesicle; ve, vitelline envelope; cp, capillary; ca, cortical alveoli. Scale bars, 10 μm (A-D, H, I, L, K, and inset in I and L), 50 μm (G, J), 20 μm (E, F), 5 μm (inset in E and F)., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361381
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361381
HANDLE: http://hdl.handle.net/10261/361381
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361381
PMID: http://hdl.handle.net/10261/361381
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361381
Ver en: http://hdl.handle.net/10261/361381
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361381

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361389
Dataset. 2024

SUPPLEMENTARY MATERIALS. A GENERALIZED VECTOR-FIELD FRAMEWORK FOR MOBILITY

  • Liu, Erjian
  • Mazzoli, Mattia
  • Yan, Xiao Yong
  • Ramasco, José J.
Supplementary Note 1: Mobility data.-- Supplementary Note 2: The statistical description of empirical trajectories.-- Supplementary Note 3: The geographical city center as RP.-- Supplementary Note 4: Relationship between ρ and the field.-- Supplementary Note 5: Unbalance of empirical trajectories in NYC and further Chinese cities.-- Supplementary Note 6: Rand model flux results.-- Supplementary Note 7: Sensitivity analysis with the d-rand model for different D(r).-- Supplementary Note 8: d-rand-d model ρ results.-- Supplementary Note 9: Sensitivity analysis for the d-mix model for R and L.-- Supplementary Note 10: The empirical mobility field.-- Supplementary Note 11: Features of mobility field with Foursquare check-ins data.-- Supplementary Note 12: Fit of the average distance ℓc for the d-mix model., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361389
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361389
HANDLE: http://hdl.handle.net/10261/361389
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361389
PMID: http://hdl.handle.net/10261/361389
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361389
Ver en: http://hdl.handle.net/10261/361389
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361389

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361390
Dataset. 2013

SUPPORTING INFORMATION: ACCELERATING THE CONTROLLED SYNTHESIS OF METAL-ORGANIC FRAMEWORKS BY A MICROFLUIDIC APPROACH: A NANOLITER CONTINUOUS REACTOR

  • Paseta, Lorena
  • Seoane, Beatriz
  • Julve, Daniel
  • Sebastián, Víctor
  • Téllez, Carlos
  • Coronas, Joaquín
Summary of synthesis conditions and yields. Crystallographic parameters of selected samples. Complementary XRD patterns for selected Fe-MIL-88B-NH2 samples obtained at different residence times. Comparison between continuous and discontinuous synthesis results. Cumulative particle size distribution of Fe-MIL-88B-NH2 at 55, 75, and 95 °C. XRD patterns of materials after moisture exposure. TGA curves of as-made and activated materials., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361390
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361390
HANDLE: http://hdl.handle.net/10261/361390
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361390
PMID: http://hdl.handle.net/10261/361390
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361390
Ver en: http://hdl.handle.net/10261/361390
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361390

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361396
Dataset. 2023

DIFFERENTIAL REGULATION OF AQP1AB2 SPLICE VARIANTS DURING OVARIAN FOLLICLE MATURATION AND HYDRATION IN VIVO [DATASET]

  • Ferré, Alba
  • Chauvigné, François
  • Zapater, Cinta
  • Finn, Roderick N.
  • Cerdà, Joan
(A) Representative immunoblots of protein extracts from follicles at the previtellogenic, vitellogenic), hydrating, and mature stage (2.5 follicle equivalents/lane) using the α-Aqp1ab2-Nt antisera. The blots show extracts from three different pools of follicles collected from three different females. The arrowheads indicate the Aqp1ab2-WT, Aqp1ab2_v1 and Aqp1ab2_v2 proteins according to their apparent molecular masses. In all blots, molecular mass markers (kDa) are on the left. (B) Relative amount of the Aqp1ab2_v1 (left) and Aqp1ab2_v2 (right) isoforms with respect to the Aqp1ab2-WT at the different development stages based on the blots shown in A. Bars (mean ± SEM; n = 3 fish) with different superscript are statistically significant (one-way ANOVA; P < 0.05)., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/361396
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361396
HANDLE: http://hdl.handle.net/10261/361396
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361396
PMID: http://hdl.handle.net/10261/361396
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361396
Ver en: http://hdl.handle.net/10261/361396
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361396

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