Resultados de investigación

The consensus sequence logos were built using the online tool WebLogo 3 [49]. The reference sequences (RefSeq) were obtained from the NCBI database, and the number of RefSeq aligned per family is indicated. The conserved invariant amino acids arginine (R) and aspartic acid (D) are highlighted within yellow boxes, and the position of the sulfur-contain amino acid (cysteine or C, and methionine or M) is highlighted in a red box, Peer reviewed

Supplemental Figure 2. Proportion of articles that reported R and Python for the derivation of EML by year, Peer reviewed

Supplemental Figure 3. Diagram of the Microsoft Access database and its collected fields., Peer reviewed

Supplemental Reference 1: Articles reporting EML for medical screening of EHRs, included in the analysis., Peer reviewed

Supplemental Reference 2: Articles reporting on ML models for medical screening of EHRs but without EML, excluded from the analysis., Peer reviewed

Supplemental Table 1. Type of studies in the selected articles, Peer reviewed

The upper part of the panel shows the genomic organization of PepMV. RdRp: RNA dependent RNA polymerase gene; TGBs: Triple gene block protein-encoding genes; CP: coat protein gene. The lower part of the panel shows the alignment of the CP region including the Cys127 codon after genotyping a PepMV-infected plant after one passage (#9), and the 8 PepCPC127S-infected plants after one passage (#1–8). The Cys127 codon is framed with a red rectangle. The Cys127 replacement was stable in all the individual passages, Peer reviewed

Supplemental Table 2. Inclusion Exclusion criteria of diseases and their classification by medical specialties, Peer reviewed

Western blot of a protein extract from N. benthamiana leaves expressing PepCPWT. Protein extract aliquots were oxidized with CuCl2 (lanes 1 and 2), reduced with dithiothreitol (lanes 3 and 4), or not pre-treated with any of them (lanes 5–6). Protein extracts loaded in lanes 2, 4 and 6 were incubated with methyl-polyethylene glycol-maleimide (MM(PEG)24) as described by Pant et al. (2021). Reduced cysteines are alkylated with MM(PEG)24 and one conjugation increases the protein mass by 1.24 kDa. A double band was observed in lanes 2 and 4, indicating partial CP oxidation, reduction or alkylation in the controls. The membrane was stripped and re-probed with an anti-Actin antibody as the loading control, Peer reviewed

Western blot of a protein extract from N. benthamiana leaves expressing PepCPWT. Protein extract aliquots were oxidized with CuCl2 (lanes 1 and 2), reduced with dithiothreitol (lanes 3 and 4), or not pre-treated with any of them (lanes 5–6). Protein extracts loaded in lanes 2, 4 and 6 were incubated with methyl-polyethylene glycol-maleimide (MM(PEG)24) as described by Pant et al. (2021). Reduced cysteines are alkylated with MM(PEG)24 and one conjugation increases the protein mass by 1.24 kDa. A double band was observed in lanes 2 and 4, indicating partial CP oxidation, reduction or alkylation in the controls. The membrane was stripped and re-probed with an anti-Actin antibody as the loading control, Peer reviewed