BUSCADOR RECOLECTA

NMR spectra; crystallographic data; IR spectrum; and DFT-calculated coordinates., Peer reviewed

Deracemization reaction courses, IC50 determination plots, immobilization parameters, amino acid sequences of the enzymes used, 1H-NMR spectra, GC, and HPLC chromatograms, chromatographic retention times of the products, and reaction schemes from the work presented here., Peer reviewed

Figures S1–S19: 11B, 1H, 31P, 1H-31P-HMBC, 1H-1H-COSY NMR spectra for 1, and for the reaction mixtures with pyridine and dimethylphenylphosphine; Table S1: calculated Cartesian coordinates for PH3-ligated model compound [Rh(η2-B3H8(H)2(PH3)2]., Peer reviewed

For each sample of the two comparisons (35S-StSP6A versus Wild Type and StSP6A-RNAi versus Wild Type) two biological replicates were prepared. In the case of 35S-StSP6A and StSP6A-RNAi each replicate was composed by a pool of several transgenic lines.-- Experiment type: Expression profiling by array., Comparisons (35S-StSP6A versus Wild Type and StSP6A-RNAi versus Wild Type., Summary StSP6A is a protein with sequence homology to the Arabidopsis FLOWERING LOCUS T (FT). FT is a main component of the long range flowering promoting signal or florigen that induces photoperiodic flowering under long days (LDs). In potato, short days (SDs) promote tuber induction, and wild subspecies like Solanum tuberosum ssp andigena (wild type line 7540) are strictly dependent on SDs to tuberize. StSP6A transcription is induced in leaves and stolons at early stages upon transfer to SD inductive conditions, priotr to visible solon swelling. Transgenic andigena lines over-expressing the StSP6A gene under the control of the 35S promoter are able to tuberize under LD non-inductive conditions, whereas silencing of this gene by means of an specific RNAi construct are strongly delayed in tuber induction in SD inductive conditions. This data indicate that StSP6A plays a central role in the control of tuber induction., Peer reviewed

All collected stolons (LD; and 2d, 4d, 6d and 8d under SD) were processed as described. Hybridization scheme with a loop design was applied to compare one sample against the rest of the samples in the induction time course.-- Experiment type: Expression profiling by array., Expression profiling analyses of early stages of tuber induction, plants of Solanum tuberosum ssp andigena (7540)., For expression profiling analyses of early stages of tuber induction, plants of Solanum tuberosum ssp andigena (7540) were used. This wild subspecies is strictly dependent on photoperiod for tuberisation, such that short days (SD) inductive conditions are required in order to trigger tuber induction in the stolons. Andigena plants were grown in the greenhouse under LD non-inductive conditions until a 10-leaf stage. They were subsequently transferred to inductive SD conditions (8 h light/16 h dark), and sampled at 0, 2, 4, 6 and 8 days after transfer to SDs. Tuber swelling was visible approximately 6-8 days after transfer to inductive conditions. The apical region of the stolons (2 cm) was collected one hour before the beginning of the light period., Peer reviewed

This dataset reports for the classical rosette bottle data for the following Essential Ocean Variables: salinity, dissolved oxygen, total alkalinity, pH, nitrates and silicic acid. It also reports on the measurements of the CTD-O2 probe at the bottle levels: pressure, temperature, salinity and dissolved oxygen, The GEOVIDE cruise was carried out coast to coast between Portugal and Newfoundland via the south tip of Greenland, following the OVIDE line in the eastern part and crossing the Labrador Sea in the western part. The classical hydrographic rosette was cast 163 times at 78 different geographical positions called stations. While the CTD-O2 probe acquired continuous profiles of the “physical” variables (pressure, temperature, salinity and dissolved oxygen), 22 Niskin bottles were closed at different levels during the upcast to provide samples for biogeochemical analysis. After calibration, we find precisions for pressure, temperature, salinity and dissolved oxygen that fit the GO-SHIP international quality requirements. In parallel, but not simultaneously, a trace-metal rosette (TMR) was cast 53 times, also acquiring profiles of physical variables, and equipped with 24 Go-Flo bottles adapted for the sampling of trace metals. Depending on the number of operations, stations were identified as “Short” (one single CTD cast), “Large” (3 CTD casts), “XLarge” (up to 6) and “Super” (up to 11). All along the track of the ship, current magnitude and direction was measured by Ship Acoustic Doppler Current Profilers, down to 1000m depth, Spanish Ministry of Economy and Competitiveness through the BOCATS (CTM2013-41048-P) project, co-funded by the Fondo Europeo de Desarrollo Regional 2014–2020 (FEDER). CNRS and the AtlantOS H2020 project under grant number 633211. LABEX-Mer (French Government “Investissement d’Avenir” programme, ANR-10-LABX-19-01), No

Table S1. Hydrodynamic size and ζ-potential of magnetic nanoparticles. Table S2. Iron concentration located inside the cells. Figure S1. Sterility assay. Figure S2. MNP toxicity assay in 2D cell cultures. Figure S3. MNP toxicity evaluated in 3D models. Figure S4. Magnetic characterization of the 3D models., Peer reviewed

(Figure S1) Characterization of the MNP glucose functionalization efficacy; (Figure S2) cell viability studies; (Figure S3) hysteresis loop simulating the experimental conditions in water measurements; (Figure S4) hysteresis loops simulating the experimental conditions in vitro; (Computational details) extended description of the calculations performed for the analysis of the local termal effects occuring during the AMF exposure; and (Figure S5) Bid expression analysis., Peer reviewed

General information for the experimental section, structural analysis of complexes 3, 5, 9a, 9b, and 11a, computational details, energies of optimized structures, observed and calculated UV–vis spectra of 3 and a and b isomers of 9–12, analysis of computed UV–vis data, theoretical analysis of molecular orbitals, cyclic voltammograms of 3 and a and b isomers of 9–12, photophysical studies, and NMR spectra (PDF). Atomic coordinates of optimized complexes (XYZ)., Peer reviewed

[Background]: Promoter hypermethylation of tumour suppressor genes is frequently observed during the malignant transformation of colorectal cancer (CRC). However, whether this epigenetic mechanism is an actual driver of cancer or is a mere consequence of the carcinogenic process remains to be elucidated., [Results]: In this work we performed an integrative multi -omic approach to identify gene candidates with strong correlations between DNA methylation and gene expression in human CRC samples and a set of 8 colon cancer cell lines. As a proof of concept, we combined recent CRISPR-Cas9 epigenome editing tools (dCas9-TET1, dCas9-TET-IM) with a custom arrayed gRNA library to modulate the DNA methylation status of 56 promoters previously linked with strong epigenetic repression in CRC, and we monitored the potential functional consequences of such DNA methylation loss by means of a high-content cell proliferation screen. Overall, the epigenetic modulation of most of these DNA methylated regions had a mild impact in the reactivation of gene expression and in the viability of cancer cells. Interestingly, we found that epigenetic reactivation of RSPO2 in the tumour context was associated with a significant impairment in cell proliferation in p53-/- cancer cell lines and further validation with human samples demonstrated that the epigenetic silencing of RSPO2 is a mid-late event in the adenoma to carcinoma sequence., [Conclusions]: These results highlight the potential role of DNA methylation as a driver mechanism of CRC and open up the venue for the identification of novel therapeutic windows based on the epigenetic reactivation of certain tumour suppressor genes., Peer reviewed