Resultados de investigación

14 pages. -- Supplementary Note 1. Time lapse movie showing evaporation of the aluminum foil mask during 100 shots. -- Supplementary Note 2. Photoluminescence (PL) and Secondary Ion Mass Spectrometry (SIMS) data correlation to PL data. -- Supplementary Note 3. Details on energy deposition and heat calculations. -- Supplementary Note 4. Details on Nuclear Reaction Analysis (NRA). -- Supplementary Note 5. Details on channeling Rutherford Backscattering (ch-RBS). -- Supplementary Note 6. Supplemental material on Density Functional Theory (DFT) calculations of G and W-centers in silicon., Peer reviewed

5 pages. -- Supplementary I: Basics of carrier recombination dynamics and time-resolved photoluminescence. -- Supplementary II: Pump power dependent optical properties. -- Supplementary III: Details of First-principles calculations and modeling of ZPL distribution with strain vectors biased in z-direction. -- Supplementary IV: Dependence of the G-center triplet transition on disorder. -- Supplementary V: Effect of vacancies and interstitials on G-center ZPL at far distances. -- Supplementary VI: Details of the strain calculation., Peer reviewed

6 pages. -- 1 figure and 2 tables. -- Figure S1. Correlation between concentration of 129I in intestional content and muscle tissues of fish collected in Fukushima waterbody Suzuuchi pond and in Chernobyl waterbodies Glubokoye lake and Staukha lake. -- Table S1. Activity concentration of 137Cs, 129I and 127I, including atomic ratios of 129I with 127I and 137Cs in different tissues of crucian carp (Carassius auratus langsdorfiicarassius) from Suzuuchi pond (n=5). -- Table S2. Fish characteristics and concentration of 129I and 127I in muscle tissue (dry weight) of individual fish collected in Fukushima waterbodies and in Chernobyl waterbodies., Peer reviewed

This study addresses the long-term dynamics of humoral immunity to two viral pathogens of the European rabbit, an endangered keystone species of major ecological relevance. Such studies are particularly challenging in free-ranging species, and a combination of longitudinal capture-mark-recapture and semi-quantitative serology was used to address this question. Over 600 normalised absorbance ratios of indirect ELISA, obtained from 505 individual rabbits in 7 populations over five years were analysed using linear mixed models. The results support a lifelong acquired humoral immunity to myxoma virus and rabbit hemorrhagic disease virus upon natural infection and suggest the presence of maternal immunity to the latter in wild juvenile rabbits. These results contribute to understanding the epidemiology of two viral diseases threatening this keystone species and assist in developing conservation programs., Fundação para a Ciência e Tecnologia, Award: SFRH/BPD/116596/2016 European Commission, Award: NORTE-01-0246-FEDER-000063 Ministerio de Universidades, Award: Margarita Salas University of Castilla-La Mancha, Award: Plan Proprio, Peer reviewed

[Content of the file SUPRACHEM_Dataset_Reverse-micelles-I _xlsx/ods] The file contains sheets that have been named according to the data presented in figures in the research article. As an example, Figure 1 RM Ms I present the data in Figure 1 expressed as mean and standard deviation, as well as the figure featured in the article. In addition, Fig 1 SPPS includes the raw data in Figure 1 and the results of the statistical analysis provided by 29.0 IBM SPSS Statistics software program., Descripción de los contenidos de los ficheros: - Archivo Excel con nombre “SUPRACHEM_Dataset_Reverse-micelles-I_xlsx.xlsx”. - Hoja de cálculo de OpenDocument con nombre “SUPRACHEM_Dataset_Reverse-micelles-I_ods.ods”. - Archivo formato rtf o README file con nombre “SUPRACHEM_Dataset_Reverse-micelles-I _README_rtf.rtf”., Los datos han sido generados utilizando Excel Office 2019 e IBM SPSS Statistics versión 29.0. Los datos SPSS han sido exportados a Excel., This dataset corresponds to those obtained in a study on the influence of reverse micelles of yolk egg phosphatidylcholine on the antioxidant efficacy of α-Tocopherol and its polar counterpart Trolox in sunflower oil. In this work, we examined the experimental conditions applied in most studies in this field and compared them to a model that was closer to an edible oil. Specifically, we studied the low antioxidant concentrations typically used in these studies, along with the lipid substrate, which is typically a stripped vegetable oil diluted in medium-chain triacylglycerol (MCT) oil. The results obtained were contrasted to a model consisting of a stripped vegetable oil (sunflower oil) with no lipid dilution and antioxidant concentrations normally found in the neat or non-stripped oil. This study has recently been published in the Journal Antioxidants (Antioxidants 2023, 12, 1993. https://doi.org/10.3390/antiox12111993) and it was developed within the framework of the SUPRACHEM project, titled "New Insights into the Loss of Antioxidant Effectiveness of Phenolic Compounds in Vegetable Oils in the Presence of Phosphatidylcholine"., SUPRACHEM (Supramolecular Interactions of Natural Antioxidants to Prolong the Shelf-Life of Foods), funded by MCIN/AEI/10.13039/501100011033 through project with reference PID2020-119304RB-I00., The data set consists of: - a numerical quantitative file saved both in .xlsx and .ods formats (“SUPRACHEM_Dataset_Reverse-micelles-I_xlsx.xlsx” and “SUPRACHEM_Dataset_Reverse-micelles-I_ods.ods”) - a README file: “SUPRACHEM_Dataset_Reverse-micelles-I _README_rtf.rtf”., Peer reviewed

Figures S1-S11. -- Supplemental Data Set 1: Arabidopsis lines used in this study. -- Supplemental Data Set 2: List of plasmids used in this study. -- Supplemental Data Set 3: List of primers and synthetic sequences used in this study for genotyping and cloning. -- Supplemental Data Set 4. Yeast strains used in this study. -- Supplemental Data Set 5. Other materials used in this study. -- Supplemental Data Set 6. Statistical analysis., Supplementary figures_3rdSbmission.pdf, tpc.22.01238Supplemental Data Sets 1XXX6.xlsx, Peer reviewed

Script to perform the MNase-seq analysis on porcine sperm., Peer reviewed

18 pages. -- Fig. S1 DNA sequence alignment of SlG1 CRISPR mutants. -- Fig. S2 Protein alignments of SlG1 wild-type sequences with the selected CRISPR 20 mutants. -- Fig. S3 SlG1, SlG2, and SlG3 transcript levels in different tissues and developmental stages. -- Fig. S4 Expression levels of genes encoding GLS and GGPPS isoforms in leaves after Pseudomonas syringae infection. -- Fig. S5 SlG1-Myc is able to specifically bind to protein partners. Fig. S6 Root parameters in SL-defective mutants. -- Methods S1 Metabolite analyses. -- Methods S2 Gene expression analyses. -- Methods S3 Co-immunoprecipitation assays. -- Table S1 List of primers used in this work. -- Table S2 Constructs and cloning details. -- Table S3 Co-expression of tomato GGPPS paralogs (guide genes) with isoprenoid-related genes (query genes) in root tissue., Peer reviewed

-Data were generetaed from February to September 2023._ -vcab_single,xml and kTsweek.xml are the input files for VCAbuilder and kTsweep, executables of the almaBTE package. The folder 'TiSe2' contains the force constants computed with DFT and other files necessary to run the calculations. File of the type of TiSe2_25_25_1_200_200.Tsweep are the output of kTsweep._ -Data were generetaed as detailed in the related article._ -Files can be opened and read with a simple text editor. The almaBTE code (freely available at https://almabte.bitbucket.io/) is needed to carry out the thermal conductivity calcualtions._, In this project we computed the thermal conductivity of two phases of monolayer TiSe2. The 2x2 is the ground state that is stable at low temperature and that feature a charge density wave (CDW). The 1x1 is the undistorted phase that is stabilized by photoexcitation (which we effectively simulate with a larger broadening of the Fermi-Dirac distribution, i.e. 0.197895 vs 0.01). Each folder contains the files necessary to carry out a thermal conductivity calculations with almaBTE (freely available at https://almabte.bitbucket.io/). The force constants were obtained with the DFT package VASP, as detailed in the related article., With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000917-S)., Thermal conductivity of (a) the CDW 2x2 phase with a Fermi-Dirac broadening of 0.01; (b) the 1x1 phase with a Fermi-Dirac broadening of 0.50; (3) the R3m phase with a Fermi-Dirac broadening of 0.197895, Peer reviewed

(A, B) Co-immunoprecipitation analysis in cells simultaneously expressing 3HA-Bfa1 and Nup159-eGFP, both in a nup42Δ or in an otherwise wild-type background, as well as in cells from another strain that concurrently expresses 3HA-Bfa1 and Nup42-eGFP. Cells expressing only 3HA-Bfa1, Nup159-eGFP, or Nup42-eGFP, as well as the wild-type strain, were also included as controls. Stationary phase cultures in YPAD were diluted to OD600 = 0.2 in fresh medium and grown for 6 h at 26°C. Western blot gel images for 3HA-Bfa1, Nup159-eGFP, and/or Nup42-eGFP are shown for both the input (INPUT) and the immunoprecipitated (Co-IP) samples. The Co-IP efficiency for 3HA-Bfa1 relative to the corresponding control with untagged Nup159 (-) and referred to the strain used as a reference (×1.00) in (B) is indicated in each case. (C) Gray scale images for each of the individual fluorescent channels in Fig 1C, which displays a positive BiFC interaction between Bfa1-VC and Nup159-VN., Peer reviewed