Resultados de investigación

10 pages. -- Figures and tables. -- Figure S1: SEM image of (a) bulk g-C3N4 and (b) ultrathin g-C3N4, (c) N2 adsorption-desorption isotherms of bCN and uCN. -- Figure S2: FTIR spectra of OAC, OLMA and TiO2 before and after ligands remove. -- Figure S3: Zeta potential distribution spectrum of TiO2 after ligands removal (a) and uCN (b). -- Figure S4: SEM image and EDS compositional maps of a T1/uCN1 composite. -- Figure S5: SEM image of T1/uCN2 and corresponding EDS spectrum. -- Figure S6: SEM image of T1/uCN2 and corresponding EDS spectrum. -- Figure S7: SEM image of T1/uCN2 and corresponding EDS spectrum; Figure S8: Chromatogram plots for 0.5 ml of standard hydrogen injected every half hour. -- Table S1: Gas Chromatography Peak Processing Data based on figure S8. -- Figure S9: Standard hydrogen curve for gas chromatography. -- Table S2: Exponential decay-fitted parameters of fluorescence lifetime of uCN, TiO2 and T1/uCN1. -- Figure S10: Photocatalytic hydrogen generation amount on bCN, TiO2 and T1/bCN1 during 4 h under simulated solar light irradiation; Table S3: Photocatalytic hydrogen production about TiO2/g-C3N4 based catalysts. -- Table S4: The AQE values with different incident light wavelengths for T1/uCN1. -- Figure S11: (a) Stability cycles of the T1/uCN1 for H2 evolution under simulated solar light irradiation; (b) TEM image of T1/uCN1 after 20 h photocatalytic H2 evolution reaction and (c) XRD pattern of T1/uCN1 before and after 20 h photocatalytic H2O2 evolution reaction., CN2 is supported by the Severo Ochoa program from Spanish MINECO (Grant No. SEV-2017-0706) and is funded by the CERCAProgramme / Generalitat de Catalunya., Peer reviewed

A) Percentage of enriched AT-II cells co-expressing the entry factors ACE2 and CD147 (in blue), and ACE2 and TMPRSS2 (in purple). (B) t-distributed Stochastic Neighbor Embedding (tSNE) representation for AXL expression in CD45+ and CD45-EpCAM+ fractions from a representative lung tissue. Right graphs show the percentage of expression of the AXL entry factor in the different cell populations, which were identified as in Fig 1A. (C) Bar plots showing the percentage of viral entry inhibition on HLT cells in the presence of anti-ACE2 antibody (15μg/ml) or recombinant human AXL (50μg/ml) after cell challenge with VSV*ΔG(Luc)-Spike. Data were analyzed by one sample t-test; *p<0.05, **p<0.01. (D) Frequency of each subset relative to live cells at 0h and 24h with and without the presence of virus. All cell subsets were identified as shown in S2A Fig. (E) EC50 values in the HLT model obtained from 3 different lung donors and performed in replicates. (F) Cells from 1 donor were cultured with 20 μM of selected drugs for 48h, and cell toxicity was measured using the CellTiter-Glo Luminescent kit (Promega), following the manufacturer’s instructions. Data was normalized to the untreated control. Mean±SEM is shown for all graphs, Peer reviewed

Fig. S1. KSR1ΔCA1 localizes to the plasma membrane and binds BRAF. Fig. S2. 3D model of the mKSR1 kinase domain. Fig. S3. Purification of recombinant KSR1 protein. Fig. S4. RAS-independent proliferation in the absence of p53 does not involve KSR. Fig. S5. Western blot analysis of KSR1 expression levels in parental and resistant MIA PaCa-2 (A) as well as PDX-dc1 (B) cell lines. Fig. S6. Model of KSR-driven proliferation in RASless cells., Peer reviewed

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Supplementary Notes 1: Case Reports. Supplementary Notes 2: 3D modeling analysis of the identified Thyroid Peroxidase (TPO), Dual Oxidase 2 (DUOX2) and Iodothyrosine Deiodinase I IYD variants. Supplementary Table 1. Human thyroid peroxidase, dual oxidase 2 and iodothyrosine deiodinase missense variants identified and their analysis with amino acid substitution prediction tools. Supplementary Table 2. Identification of human Thyroid Peroxidase (TPO) variants by Next-Generation Sequencing (NGS). Supplementary Table 3. Identification of human Dual Oxidase 2 (DUOX2) variants by Next-Generation Sequencing (NGS). Supplementary Figure 1. Differential diagnosis in patients with thyroid dyshormonogenesis. Supplementary Figure 2. Partial protein alignment of the Homo sapiens, Camelus dromedarius, Rattus norvegicus, Mus musculus, Culex quinquefasciatus, Sus scrofa, Lynx Canadensis, Xenopus tropicalis and Canis lupus familiaris thyroid peroxidase (TPO) species. Supplementary Figure 3. Partial protein alignment of the Homo sapiens, Mustela erminea, Sus scrofa, Mus musculus, Lynx canadensis, Bos taurus, Gallus gallus, Pan troglodytes dual oxidase 2 (DUOX2) species., Peer reviewed

(A) General gating strategy used to identify different cell subsets in lung samples. A gate based on FSC vs. SSC was followed by doublet and dead cells exclusion. From live CD45- cells, endothelial cells (CD31+, purple) and epithelial cells (EpCAM+, grey) were gated, and within epithelial cells, AT-II cells (EpCAM+ and HLA-DR+, pink) were identified. Out of live CD45+ cells and based on FSC vs. SSC, we identified a lymphocyte population in which we distinguished between non-T lymphocytes (turquoise) and T cells (dark green) based on CD3 expression; and big cells, where we identified three major subsets based on their expression of CD11b and CD11c and, subsequently, CD14 and HLA-DR markers. We identified alveolar macrophages (blue), monocytes (violet), myeloid dendritic cells (mDCs, fuchsia) and neutrophils (orange)., Peer reviewed

(A) Representative tSNE maps showing concatenated flow cytometry standard files for three different protocols based on different digestion enzymes (collagenase, liberase or trypsin) from total live cells (upper), CD45+ cells (middle) and CD45- cells (lower). (B) Bar plots showing cell-type composition (count) analyzed by flow cytometry for each tissue protocol. (C) Representative flow cytometry plots showing Surfactant Protein C (SPC) staining and its respective fluorescence minus one (FMO) control. (D) Representative flow cytometry plots showing ACE2 staining and its respective fluorescence minus one (FMO) control., Peer reviewed

General gating strategy used to evaluate the expression of inflammatory molecules in lung samples. A gate based on FSC vs. SSC was followed by doublet and dead cells exclusion. From live CD45+ cells and based on FSC vs. SSC, we identified lymphocyte population and big cells, in which we identified two subsets based on their expression of CD11b and CD14: myeloid CD11b+CD14+ cells (blue-green) and myeloid CD11b+CD14- cells (orange) are shown, Peer reviewed

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