Resultados de investigación

1 -xlsx File (19.0 kB). Under Commons Attribution 4.0 International., Field data from four Mediterranean shrublands monitored during an extreme drought in 2014 (hydrological year) in SE Spain. Data are plant dieback of the total plant community and main plant biotypes (shrubs, subshrubs and grasses), vegetation structure attributes (phytovolume, plant height, LAI, and sizes of patch and inter-patch areas), soil properties (soil depth and soil surface cover types) and water availability indicators (soil moisture and environmental variables)., Peer reviewed

(A) CBK1-GFP cdc15-2 (YMF3566) and CBK1-GFP TOR1-1 cdc15-2 (YMF3565) cells were grown in YPD and arrested in late anaphase by shifting the temperature to 37 °C before the addition of rapamycin for 20 min to both strains. In addition, DMSO was added to one half of CBK1-GFP cdc15-2. Untagged cdc15-2 (YMF3580) and TOR1-1 cdc15-2 cells (YMF3578) were used as controls. Cell extracts were prepared before the immunoprecipitation of Cbk1-GFP on ChromoTek GFP-Trap Magnetic beads. Isolated material was subjected to analysis by mass spectrometry. Numbers represent spectral average of key Cbk1 functional-related interactors in 3 independent replicates of the experiment. The average is calculated as the sum of all spectral counts for the 3 replicates divided by 3. We found Cbk1 interactions previously described: Mob2 [48] or components of the RAM pathway such as Tao3 [97], Sog2 [98], and Kic1 [99]. Besides, Cbk1 negative regulators Lre1 and Fir1 were also identified [5,43]. Besides, MS found Myo1, a component of the actomyosin ring [43]. Our results showed that Cbk1 interacts with the transcription factor Ace2 [47,48]. Moreover, Cbk1 was bound to Ssd1, an RNA-binding protein that represses the translation of cell wall remodelling proteins until Ssd1 is phosphorylated by Cbk1 [48,100]. Interestingly, TORC1 and Ssd1 have been described to collaborate to maintain cellular integrity [73]. We found interactions with paralogs serine/threonine kinases Kin1 and Kin2 [74,75]. Finally, we detected protein Yol036w of unknown function interacting with Cbk1 [40,74]. Details of other known Cbk1 interactors are shown in S2 Table. (B) CBK1-5FLAG ACE2-HA cdc15-2 (YMF3585) and control cells (YMF3587) (i), CBK1-5FLAG MOB2-9MYC cdc15-2 (YMF3838) and control cells (YMF3835) (ii), or CBK1-TAP SOG2-6HA cdc15-2 (YMF4235) and control cells (YMF4236) cells (iii) were grown in YPD and arrested in late anaphase by shifting to 37 °C before the addition of rapamycin for 20 min when indicated. Subsequently, protein extracts were prepared and immunoprecipitations of Cbk1 on FLAG-beads or IgG beads (as denoted) were performed before the detection of the indicated proteins by immunoblotting. Raw data for blots can be found in Supporting information (S7 Raw Images). (C) Components of TORC1 were found in the same mass spectrometry analysis as in A. Numbers represent spectral average of 3 independent replicates of the experiment., pbio.3002263.s008.pdf, Peer reviewed

(A) Orthologues of S. cerevisiae Sec3 in the indicated fungal species were identified by PSI-BLAST searches, and a CLUSTAL O multiple sequence alignment was performed. Conserved NDR/LATS phosphorylation consensus is marked with a red square following consensus in S. cerevisiae [47] and in S. pombe [54]. Putative Cbk1 phosphorylation sites were identified in Sec3 by scanning the sequence (S18, S32, S43, and S66). (B) To confirm that Sec3 was essential in the budding yeast strain used in this work, we deleted SEC3 in diploid cells (YMF4159). Spores that lack Sec3 were able to grow extremely deficiently, although they formed tiny colonies (i). To conditionally inactivate Sec3, we planned to follow the same strategy as we did for the study of Cbk1 mutants that is explained in detail in S2E Fig. We added the “auxin inducible degron” (“aid”) cassette [38] to the C-terminal end of SEC3. Then, control cells (ADH-TIR1; YJW15) and sec3-aid ADH-TIR1 cells (YMF4612) were grown at 24 °C on YPD medium before serial dilutions of 50,000, 5,000, 500, and 50 cells were plated on the indicated media and incubated for 3 days (ii). sec3-aid ADH-TIR1 cells were able to grow under restrictive conditions at 24 °C (ii), which showed that Sec3 depletion was not completely effective. This prevented us from the use of sec3-aid mutant, in combination with cdc15-2 to inactivate Cdc15 function at 37 °C, and test whether phosphomimetic version of Sec3 (Sec3-4E, S18E, S32E, S43E, and S66E) was able to rescue cell separation defects associated to cdc15-2 cells. (C) To be able to follow version of t-SNARE Sso1 that lacks N-terminal autoinhibition domain, we deleted DNA sequence that corresponds to amino acids 1–146. At the same time, we fused the tag 5xFLAG at the N-terminal of the truncated Sso1 to be able to follow the protein dynamics. As control, we tagged the N-terminal end of wt Sso1 with 5xFLAG. To determine whether 5xFLAG-Sso1 and 5xFLAG-Sso1Δ1–146 were functional, we combined 5xFLAG-SSO1 (i) or 5xFLAG-sso1Δ1–146 (ii) with sso2Δ. In both cases, harbouring 5xFLAG version of Sso1 and sso2Δ was lethal, which indicates that addition of 5xFLAG disturbed Sso1 function. Individual deletions of SSO1 and SSO2 are alive, but inactivation of both t-SNARE Sso proteins is lethal., pbio.3002263.s009.pdf, Peer reviewed

1 file, Supplementary information for the article https://doi.org/10.1038/s41598-020-57501-0, Table S1. Primer sequences used for qPCR.-- Supplementary Figure S1. Antiviral activity of the CRP1-7-depleted supernatants.-- Supplementary Figure S2. Assessment of the ability of CRP-mix to induce the transcription of ifnphi1 and ifnphi2 in vitro.-- Supplementary Figure S3. Assessment of the ability of IL-6 to induce the expression of crp1-7 transcripts in vivo.-- Supplementary Figure S4. Transcriptional modulation of autophagy by SVCV in the ZF4 cells.-- Supplementary Figure S5. Effect of the addition of exogenous cholesterol on intracellular autophagosome levels and SVCV infection in vitro, Peer reviewed

Documents: Chesas_Thin_sections_scans.zip Chesas_Fig.captions1991-01.xlsx, We show data of soils developed on the outcropping gypseous nucleus of the Barbastro-Balaguer anticline, NE Spain. Chesas is the local name for the lands of this outcrop that stand out by their whitish tones. The data collected here come from soils of the municipalities —from West to East— of Peraltilla, Almunia de San Juan, Tamarite, Torá, and Iborra. The analytical methods plus details of the landscape and sampling sites can be found in: (i) the book entitled “Morfología y génesis de suelos sobre yesos” (http://hdl.handle.net/10261/84695), and (ii) the article entitled “Salada Farrachuela, a saline wetland in Tamarite de Litera, Spain” (DOI: 10.29077/bol.114.ce05.herrero). Here we present a compressed zip file with 302 TIFF images corresponding to 151 thin sections of these soils. Most of the sections —with the prevalent size of 13.5 × 5.7 cm— were manufactured by the first author in the Institut National Agronomique de Paris-Grignon under the technical supervision of Mr. P. Guilloré, in the context of a scholarship granted by the Government of France for working in the lab of Dr. N. Fédoroff. The surviving thin sections from chesas are archived and scanned at the EEAD-CSIC in Zaragoza, Spain. Their scans are compiled in the file “Chesas_Thin_sections_scans.zip”. Several dozen micrographs of the chesas thin sections appeared in the abovementioned book published in 1991 (http://hdl.handle.net/10261/84695). The file “Chesas_Fig.captions1991-01.xlsx” presents a tentative English translation of the 154 Figure captions for the micrographs and the other figures in the book., We acknowledge the grant PID2021-127170OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”, and the grant TED2021-130303B-I00 funded by MCIN/AEI/10.13039/501100011033 and by the “European Union NextGeneration EU/PRTR”., Peer reviewed

1 .docx File., The Monegros region, located in the Central Ebro Valley (CEB) expands on parts of the conterminous provinces of Huesca and Zaragoza, Spain. Outstanding traits of Monegros are the aridity and the abundance of gypsum in the soils. Moreover, the Miocene lutites contain other salts more soluble than gypsum. The salts often are evapoconcentrated in closed depressions, locally named saladas in the municipalities of Sástago and Bujaraloz. The waters at these athalassohaline playa-lakes and other small depressions use to be more saline than the sea, and their soils are both gypseous and hypersaline. Withal, the saladas are threatened shelters of biodiversity that have been studied by naturalists from years ago. We present a dataset of disperse references gathering a bunch of natural traits of these saladas., PID2021-127170OB-100 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”, and the grant TED2021-130303B-I00 funded by MCIN/AEI/10.13039/501100011033 and by the “European Union NextGeneration EU/PRTR”., Peer reviewed

This dataset contains structural data of the southern sector of the Sierra de Reyes Antiform along the Andean front in the Neuquén Basin, Argentina. This structural data consist of bedding dips and their strike as well as Remote Sensing Mapping of the main units outcropping in the area. Location of calcite shells within the Vaca Muerta-Quintuco System and Mulichinco Formation for Sr isotopic dating is also included., This work was funded by a collaborative knowledge transfer project between the CSIC and Equinor Research Center in Bergen (Norway), MAPA project (PIE–CSIC–202430E005), and the Grup de Recerca Reconegut per la Generalitat de Catalunya “Modelització Geodinàmica de la Litosfera” (2021 SGR 00410), Neuquén Basin.KMZ, Peer reviewed

102 pages, Supplementary information for the article https://doi.org/10.1093/bioinformatics/bty736.-- Computational settings. Histograms and dispersion plots of the multi-starts of local searches (MS). Convergence curves of eSS and MS. Tables and figures. Appendix: adjoint vs forward sensitivities, Peer reviewed

3 files, Additional file 1. Remarks on the eSS optimisation solver and detailed results for the Goodwin Oscillator problem and additional ENSO contour plots. This file describes our modifications to the eSS global optimisation solver. It also contains tables and figures showing the detailed results for the Goodwin Oscillator problem, and additional contour plots for the ENSO example.-- Additional file 2. Critical comparison of optimisation solvers. In GEARS, the optimisation problems are solved using the hybrid metaheuristic eSS. A comparison of the eSS global optimisation solver used in GEARS with other competitive local and global optimisation solvers.-- Additional file 3. Detailed results for the Fitzhugh-Nagumo, Repressilator and Enzymatic Oscillator problems. This file contains tables and figures showing the detailed results for the Fitzhugh-Nagumo, Repressilator and Enzymatic Oscillator problems, Peer reviewed

(A) Our findings suggest that TORC1 blocks Cbk1 kinase activity in the absence of Cdc15 function while cells are arrested in late anaphase at 37 °C, which would promote a defect in the following cell separation as secretory vesicle transporting hydrolases would be unable to fuse into the plasma membrane. (B) Rapamycin inhibits TORC1 and would induce the accumulation of a hypophosphorylated version of Cbk1 that would be able to phosphorylate its key substrates as the exocyct component Sec3, which regulates the function of the SNARE complex at the site of division to promote fusion of secretory vesicle at the plasma membrane. Hydrolases contained in secretory vesicle are released to promote cell separation., Peer reviewed