BUSCADOR RECOLECTA

Lápida situada en la Capilla Mayor. Mármol; texto en mitad superior; en la inferior capelo episcopal sin más elementos. Texto y capelo enmarcados por una línea simple., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

Capilla mayor. Inscripción de mármol., CSIC. Proyecto intramural: “Epigrafía latina inédita de los siglos XV al XVIII en monumentos civiles y eclesiásticos de la provincia de Cuenca (2006-2007). Referencia: 2006 | 0| 011., Peer reviewed

Peer reviewed

Supplementary Figure 1-6 Supplementary Table 1-4 Supplementary Material and Methods, Peer reviewed

6 pages. -- The following Supporting Information is available for this article: Fig. S1. Transcript abundance of the indicated genes in GFP and crtB samples at 96 hpi. -- Fig. S2. Changes in qP and qL during chromoplastogenesis in W50 and W500 samples. -- Table S1. Accessions of N. benthamiana genes encoding isoprenoid biosynthetic enzymes. -- Table S2. Accessions of N. benthamiana genes encoding plastid proteins. -- Table S3. Primers used in this work., Peer reviewed

Figures and tables. -- Supplementary Figure 1. Sequence analyses for CsALC. -- Supplementary Figure 2 Comparison of WT and Csalc mutants in plant growth, fruit length and pollen viability. -- Supplementary Figure 3 Comparison of pollen tube extension in WT and Csalc mutants. -- Supplementary Figure 4 Transcriptomic analyses of Csalc mutant and WT ovaries. -- Supplementary Figure 5 Phylogenetic and sequence analyses for CsRALF4 and CsRALF19 gene. -- Supplementary Figure 6 qPCR analysis of CsRALF4/19 and CsSPT in Csalc mutants. -- Supplementary Figure 7 CsALC promoted CsRALF4/19 expression in vivo. -- Supplementary Figure 8 The region surrounding CsALC gene was selected during cucumber domestication. -- Supplementary Figure 9 The comparison of pollen tube extension in Csspt and Csalc mutants. -- Supplementary Data 1: Primer information. -- Supplementary Data 2: SPT and ALC homologues among angiosperms. -- Supplementary Data 3: DEGs between Csalc mutants and WT. -- Supplementary Data 4: G-box distribution in Csalc DEG promoters., 41467_2023_35936_MOESM1_ESM.pdf, 41467_2023_35936_MOESM2_ESM.pdf, 41467_2023_35936_MOESM3_ESM.xlsx, 41467_2023_35936_MOESM4_ESM.xlsx, 41467_2023_35936_MOESM5_ESM.xlsx, 41467_2023_35936_MOESM6_ESM.xlsx, Peer reviewed

Overall design: Ovaries of wild type and Csalc mutant at 32 h after pollination were collected for RNA-seq analysis. Three biological replicates were performed. -- Organism: Cucumis sativus. -- Experiment type: Expression profiling by high throughput sequencing., Resources available on the publisher's site: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE182139, Cucumber is an important vegetable crop worldwide, which is mainly propagated by seed. The successful occurrence of double fertilization is the premise of seed production in angiosperms. Cucumber ALC homolog (CsALC) displayed a novel and positive function in pollen tube emergence process, and regulated female fertility by promoting CsRALF4/19 function during pollen tube guidance in cucumber., Peer reviewed

32 pages. -- Figures and tables. -- Figure S1. Analysis of chalcone synthase structure for identification of substrate binding sites (PDB ID: 1CGK). -- Figure S2. Sequence alignment (Sievers et al., 2011) between Chalcone synthase (CHS) from Medicago sativa and Petunia hybrida. -- Figure S3. Measurement of intra- and extracellular concentrations of chalcone and naringenin in cells. -- Figure S4. Dependence of naringenin concentration and fluorescence intensity on the expression level of chs. -- Figure S5. Evaluation of naringenin production of mutants of each cluster with higher fluorescence than the parental strain in multi-well plates. -- Figure S6. Evaluation of beneficial mutants of four clusters in 3-mL test tubes which produced higher naringenin concentrations than the parental strain in multi-well plates. -- Figure S7. Evaluation of mutant libraries with beneficial mutations of each cluster in multi-well plates. -- Figure S8. Evaluation of beneficial mutants in 3-mL test tubes which produced higher naringenin concentrations than the parental strain in multi-well plates. -- Figure S9. Evaluation of enriched mutants through FACS-based screening. -- Figure S10. Accumulation of acetic acid in the parental strain and the CHSopt. Acetic acid production of the parental strain. -- Figure S11. Docking interactions of naringenin in CHS enzyme. -- Figure S12. Analysis of variants with the mutations of cluster 4. -- Figure S13. Effects of the repression of fabHDG expression on cell growth and naringenin production. -- Figure S14. Validation of the naringenin-production capacity of Parental-FRU3, CHSopt-FRU1, 2, 4, and 5 in flask cultures. -- Table S1. Previous studies on naringenin biosynthesis in similar cultivation conditions. -- Table S2. Strains and plasmids used in this study. -- Table S3. Oligonucleotides used in this study. -- Table S4. Information of T7 mutant promoters used in Fig S4. -- Table S5. Rational design of mutant libraries for four clusters in chs gene based on conservation of amino acids in chs and information of chalcone synthase homologs. -- Table S6. Sequence information of screened mutants from each cluster in chs through plate-based screening in Fig. S4. -- Table S7. Sequence information and naringenin production of screened variants from mutant libraries with combinations of beneficial mutations of each cluster through plate-based screening in Fig. S8. -- Table S8. Analysis of sequence information of screened variants through FACS-based screening in Fig S9. -- Table S9. Kinetic parameters of T132G and T132S/S133C in the CHS mutants. -- Table S10. Docking simulations summary. -- Table S11. 5'-UTR sequences and predicted expression levels of UTR variants used in this study., Peer reviewed

Supplementary Figure S1. Respiration rate (RR) and ethylene production (EP) in Near-Isogenic Line SC3-5-13 (mean ± SE, n=3) during postharvest ripening at 21ºC and relative humidity of 66±6% during 10 days (season 2). -- Table S1. List of molecular markers used in this study and located in MELOGEN v4.0 (Castanera et al., 2020). -- Table S2. Melon graphical genotype of the NILs used in this study. -- Table S3. List of genes, description and properties obtained from MELOGEN database v4.0 that were covered by molecular markers in the region of the QTL of the climacteric ETHQB3.5. -- Table S4. Chemical and sensory attributes of the main melon fruit volatile compounds used for QTL mapping in melon chromosome 3 identified in the five melon Near-Isogenic Lines (NILs) and the parental control ‘Piel de Sapo’ (PS) as reported in Table 2. -- Table S5. Main fruit volatile compounds identified in the headspace from the non-climacteric Near-Isogenic Line SC3-5-7 and the parental line ‘Piel de Sapo’ (PS) (n=5 and n=21, respectively) for QTL mapping in melon chromosome 3. -- Table S6. Chemical and sensory attributes of each group of the individual volatile compounds obtained in the first five groups of the correlation network analysis (Fig. 4; Table 5), Dos-Santos Figure S1. RR EP SC3-5-13 final2.pdf, Dos-Santos Tables S1-S6 FOODSv20FINAL.xlsx, Peer reviewed

Bibliometric data from Scopus about the 800 prominent researchers in the study. Each folder corresponds to a field and contains two archives: - Scopus.csv : The name, Scopus ID, Affiliation, Total number of publications and Total number of collaborators of all the authors of the field - dfPapers.csv : The title, Number of citations, authors ids and year of publication of all the papers (non repeated) in the field, Peer reviewed