BUSCADOR RECOLECTA

8 pages. -- The file includes 3 tables and 5 figures. -- Table S1. Mean and standard error of the concentrations (mg of tartaric acid equivalents/g DW of plant) and percentages of specialized compounds and nutritional traits. -- Table S2. Results of Kruskal Wallis and Dunn tests on C and N of adult seagrass leaves and flowers (n=6), and seedling leaves (n=10). SD indicates seedling leaves, Finflorescences, L1 young leaves and L3 old leaves. In Dunn test, parentheses indicate that these tissues are not statistically different to the ones next to them. -- Table S3 Loading values of PCA from figure 2. -- Figure S1. Concentration (mg chicoric acid equivalents/g DW of plant) of total phenolic compounds expressed as the set of specific phenolic compounds from the different tissues analyzed; Inflorescences (F), young leaves (L1) and old leaves (L3) from non-reproductive plants, and young leaves (RL1) and old leaves (RL3) from reproductive plants, and seedling leaves (SD). Compounds labelled as 1 (chicoric acid), 2 (caffeoylferuloyltartaric acid), 5 (di-p-coumaroyltartaric acid,), 4 (coutaric acid,), and 3 (p-coumaroylcaffeoyltartaric acid). Error bars indicate standard error of total content of phenolic compounds. For F, L1, L3, RL1, RL3 n=6 for SD n=10. -- Figure S2. Pairwise correlation matrix of total phenolic compounds (TPC), C/N, C, N, 1 (chicoric acid), 2 (caffeoylferuloyltartaric acid), 3 (p-coumaroycaffeoyltartaric acid), 4 (coutaric acid), and 5 (di-p-coumaroyltartaric acid) from all analyzed tissues (inflorescences, young leaves, old leaves and leaves from seedlings). Numbers indicate Pearson correlation coefficients. -- Figure S3. Scheme of (A) adult Posidonia oceanica in reproductive stage and (B) seedling showing the different parts.-- Figure S4. Extracted ion chromatograms for compounds (1-5) and their MS/MS spectra. -- Figure S5. Diagnostic MS/MS fragmentation for compounds (1-5), Peer reviewed

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

7 pages. -- The file includes 7 figures. -- Figure S1. Selection of the combination of Hs and Tp values used in the 1D SWASH numerical simulations. The density plot represents the frequency that a combination of Hs and Tp is given in the intertropical areas ( 25 latitude, region where most of the coral reef islands are found) of the CFSR hindcast CAWCR Global wind-wave data set[1]. The black contour represents the area containing the maximum frequency and enclosing 90% of the Hs and Tp combinations. Taking advantage of previous experience, the 60 different combinations used for the simulations (black dots) has been chosen to include the most frequent combination as well as the extremes following lines of constant Hs Tp (dotted contour lines). -- Figure S2. Dependence of the wave setup with: a) significant wave height multiplied by peak period; b) sea level; c) reef length; d) island height; and e) Manning’s roughness coefficient. The central value of each box plot represents the median value, the lower (upper) limit of the box is the 25% (75%) quantile and the whiskers show the range of values between the 5% and 95% quantiles. -- Figure S3. Sensitivity tests for adjusted wave setup parameters in equation 3. Each parameter is randomly changed by up to 5% of its value and the rest are adjusted. Histograms represent the results of the process repeated 10000 times. -- Figure S4. Dependence of the flooding with sea level and island height for incoming waves with Hs = 4m and Tp = 20s, a reef length of 500 m and a Manning friction coefficient of 0.1. The colorplot is a bi-linear interpolation from the 36 values (black dots) obtained from the numerical simulations. The horizontal thick black line indicates the flooding that an island with 1.65 m would suffer with sea-level rise is no action is taken. The dashed black line indicates the evolution of the island height with sea-level rise if present-day flooding is required to remain constant. Thin black line indicate the isoline of a useddefine flooding threshold (in this case 0.1m3/s). The intersection between this line and the horizontal line (that shows the island height) indicates the sea level at which the user defined flooding threshold is over-passed. -- Figure S5. Same as Fig. 4 but with a Manning friction coefficient of 0.05. -- Figure S6. Number of island wave-induced flooded by more than 0.1m3/s with 0.5 m of mean sea level under different return periods and for different Manning friction coefficient. The total number of flooded islands for each return period is highly dependent on the Manning coefficient. -- Figure S7. Comparison between the flooding results from our simulations (Manning friction coefficient fixes to 0.1) with the flooding threshold derived by [2] (black dashed line in each panel). Note that we used different mean sea levels while keeping constant to 1 m the reef depth, whereas [2] changed their reef depth (Dree f ) and kept mean sea level as a constant value. Thus, to perform the comparison we computed the different reef depths as 1 m plus the corresponding mean sea level value. Consequently, our island height is transformed as the original island height minus the sea level to be consistent. Colours (in logarithmic colour scale) indicate the volume of flooding (in m3/s per linear meter of coastline). Our flooding values above the threshold are always below 0.01 m2/s., Peer reviewed

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed

The PDF file includes: Supplementary Figs. S1 to S31. -- Tables S1 to S50 in the Excel files., Peer reviewed

Improved bioinformatics tools for annotating gene function are becoming increasingly available, but such information must be considered theoretical until further experimental evidence proves it. In the work reported here, the genes for the main components of the translocons of the outer membrane of chloroplasts (Toc) and mitochondria (Tom), including preprotein receptors and protein-conducting channels of N. benthamiana, were identified. Sequence identity searches and phylogenetic relationships with functionally annotated sequences such as those of A. thaliana revealed that N. benthamiana orthologs mainly exist as recently duplicated loci. Only a Toc34 ortholog was found (NbToc34), while Toc159 receptor family was composed of four orthologs but somewhat different from those of A. thaliana. Except for NbToc90, the rest (NbToc120, NbToc159A and NbToc159B) had a molecular weight of about 150 kDa and an acidic domain similar in length. Only two orthologs of the Tom20 receptors, NbTom20-1 and NbTom20-2, were found. The number of the Toc and Tom receptor isoforms in N. benthamiana was comparable to that previously reported in tomato and what we found in BLAST searches in other species in the genera Nicotiana and Solanum. After cloning, the subcellular localization of N. benthamiana orthologs was studied, resulting to be identical to that of A. thaliana receptors. Phenotype analysis after silencing together with relative expression analysis in roots, stems and leaves revealed that, except for the Toc and Tom channel-forming components (NbToc75 and NbTom40) and NbToc34, functional redundancy could be observed either among Toc159 or mitochondrial receptors. Finally, heterodimer formation between NbToc34 and the NbToc159 family receptors was confirmed by two alternative techniques indicating that different Toc complexes could be assembled. Additional work needs to be addressed to know if this results in a functional specialization of each Toc complex., Peer reviewed