BUSCADOR RECOLECTA

S4 Fig. edem response to thapsigargin. (A) Quantitative RT-PCR measurements of edem mRNA levels in WT young animals under non stress (NS) and treatment with 5 μM thapsigargin (TG), (n = 3 independent experiments). (B) Percentage of eggs that developed into L4 larvae after 3 days on 10 μM thapsigargin. Each strain was scored in three independent experiments in triplicates. (C) Quantification of WT (EV) or RNAi-treated day one adults carrying Phsp-4::GFP transgene, treated or not with 5 μM thapsigargin. The worms were kept on RNAi plates for one generation before exposure to ER stress treatments. Values represent mean fluorescence/µm2 x 1000. The red bars indicate the average ±SEM. (D) Analysis of xbp-1 spliced/unspliced ratio from young adults of indicated strains. Total RNA was isolated, reverse transcribed and used for PCR analysis of xbp-1 splicing forms., Peer reviewed

S1 Table. Strains used in this study., Peer reviewed

S2 Table. Primers used in this study., Peer reviewed

S3 Table. Raw numerical data of all the figures., Peer reviewed

Table S1. Treatment allocation based on the molecular profile. Table S2. Median PFS and OS and parameters for exponential extrapolation. Table S3. Dosage and estimated monthly costs of the treatments included in the analysis. References only cited in the supplementary material., To assess the cost-effectiveness of using next-generation sequencing (NGS) compared to sequential single-testing (SST) for molecular diagnostic and treatment of patients with advanced non-small cell lung cancer (NSCLC) from a Spanish single-center perspective, the Hospital Universitario Virgen del Rocio (HUVR). A decision-tree model was developed to assess the alterations detection alterations and diagnostic cost in patients with advanced NSCLC, comparing NGS versus SST. Model inputs such as testing, positivity rates, or treatment allocation were obtained from the literature and the clinical practice of HUVR experts through consultation. Several sensitivity analyses were performed to test the robustness of the model. Using NGS for molecular diagnosis of a 100-patients hypothetical cohort, 30 more alterations could be detected and 3 more patients could be enrolled in clinical-trials than using SST. On the other hand, diagnostic costs were increased up to €20,072 using NGS instead of SST. Using NGS time-to-results would be reduced from 16.7 to 9 days. The implementation of NGS at HUVR for the diagnostic of patients with advanced NSCLC provides significant clinical benefits compared to SST in terms of alterations detected, treatment with targeted-therapies and clinical-trial enrollment, and could be considered a cost-effective strategy., This work was funded by Roche Farma S.A., Peer reviewed

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Intracellular trafficking through the secretory organelles depends on transient interactions between cargo proteins and transport machinery. Cytosolic coat protein complexes capture specific luminal cargo proteins for incorporation into transport vesicles by interacting with them indirectly through a transmembrane adaptor or cargo receptor. Due to their transient nature, it is difficult to study these specific ternary protein interactions just using conventional native co-immunoprecipitation. To overcome this technical challenge, we have applied a crosslinking assay to stabilize the transient and/or weak protein interactions. Here, we describe a protocol of protein cross-linking and co-immunoprecipitation, which was employed to prove the indirect interaction in the endoplasmic reticulum of a luminal secretory protein with a selective subunit of the cytosolic COPII coat through a specific transmembrane cargo receptor. This method can be extended to address other transient ternary interactions between cytosolic proteins and luminal or extracellular proteins through a transmembrane receptor within the endomembrane system., Peer reviewed

[How to contribute] For any problems downloading the data or for additional information, please send us an email and we will provide you with the necessary information. We would also appreciate your feedback regarding potential errors., [Ethics statement] All procedures were conducted according to relevant Spanish national (Decreto 105/2011, 19 de abril) and regional guidelines. Necessary permits for hoopoe manipulation were provided by Consejería de Medio Ambiente de la Junta de Andalucía, Spain (Ref: SGYB/FOA/AFR/CFS and SGMN/GyB/JMIF). All applicable guidelines for the care and use of animals were followed., The research group received funds from the projects PID2020-117429GB-C21 and PID2020-117429GB-C22, funded by the Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación/10.13039/501100011033 and by “Fondo Europeo de Desarrollo Regional, a way of making Europe”. We also benefited from facilities, including an apartment, provided by the city authorities of Guadix., Peer reviewed

16 pages. -- PDF file includes: 1.Characterization. -- 2. Electrocatalysis measurement. -- 3. Photocatalytic reduction of oxygen to hydrogen peroxide. -- 4. RRDE test. -- 5. Computational method. -- 6. Apparent quantum yield (AQY) calculations. -- 7. Structural characterization. -- 8. Elemental analysis. -- 9. Band structure. -- 10. Surface area and porosity. -- 11. Calibration for H2O2 quantification. -- 12. Photocatalytic activity. -- 13. Linear sweep voltammetry. -- 14. DFT calculation results. -- 15. Reaction mechanisms., Peer reviewed

62 pages. -- PDF file includes: 1. Experimental section. -- Figure S1. XRD patterns of pure ZIF-67 (a), Co(OH)2 (b) and Co3O4 (c). -- Figure S2. SEM images of Co3O4 single-shelled nanocages and EDS chemical mapping. -- Figure S3. SEM images of and EDS chemical mapping of CoP-HS (a); CoP2-HS (b); CoP3-HS (c). -- Figure S4. Nitrogen absorption–desorption isotherms and pore size distributions of three cobalt phosphides, CoP-HS (a); CoP2-HS (b); CoP3-HS (c). -- Figure S5. XPS spectra of the XPS full scan for CoP-HS, CoP2-HS and CoP3-HS. -- Figure S6. The CV curves of CoP-HS, CoP2-HS, and CoP3-HS obtained at the 1st (a), 3rd (b), 5th (c), and 10th (d) cycles at a scan rate of 10 mV/s in a 1.0 M KOH solution. -- Figure S7. The OER activities of CoP-HS, CoP2-HS and CoP3-HS were tested by both forward and reverse scan. -- Figure S8. (a) The CV of the CoPx. (b) The double layer capacitance (CDL) was determined as the half of the slope from the plot of the capacitive current vs. scan rate plot. -- Figure S9. Chronopotentiometry responses of activity stabilized CoPx in 1.0 M KOH in the catalytic turnover region. -- Figure S10. (a-c) OER LSV curves with (red) and without (blue) 100% iR drop correction. (d) Corresponding Tafel lines. -- Figure S11. SEM images of CoP-HS (a), CoP2-HS (b) and CoP3-HS (c) single-shelled nanocages after 100 h OER stability measurement. (d) The changed ratio of Co:P before and after stability test. -- Figure S12.SEM of post-OER CoP (a) before HCl wash, (b) after HCl washed. -- Figure S13. The LSV curves of CoP-HS, CoP2-HS, CoP3-HS, Co3O4-HS and Co(OH)2-HS measured in 1.0 M KOH solution toward OER at a scan rate 10 mV/s after activation by 50 CV cycles between 0.0 V and 0.85 V (vs. Hg/HgO) at a scan rate 50 mV/s. -- Figure S14. (a) The LSV curves of carbon paper measured in 1.0 M KOH toward HER at scan rate 10 mV/s. (b) The data of CoP-HS, CoP2-HS and CoP3-HS test in 1.0 M KOH. -- Figure S15. Chronopotentiometry responses of activity stabilized CoPx in 1.0 M KOH in the catalytic turnover region. -- Figure S16. (a-c) HER LSV curves with (red) and without (blue) 100% iR drop correction. (d) Corresponding Tafel lines. -- Figure S17. The CV curves of CoP-HS, CoP2-HS and CoP3-HS measured in 1.0 M KOH solution for 1st (a), 3rd (b), 5th (c), and 10th (d) cycles at a scan rate 10 mV/s. -- Figure S18. (a) The XPS spectra, and (b) the SEM image and EDS chemical mapping of CoP-HS after 100 h HER stability measurement in 1 M KOH. -- Figure S19. TEM images of CoP-HS after HER stability test (a). Elements mapping and SAED of CoP-HS after HER stability test (b-f). -- Figure S20. (a) The LSV curves of CoP-HS, CoP2-HS, CoP3-HS and Pt/C measured in 0.5 M H2SO4 toward HER at scan rate 10 mV/s. (b) The corresponding Tafel plots for the samples in 0.5 M H2SO4. (c) Nyquist plots of CoP-HS, CoP2-HS, CoP3-HS in 0.5 M H2SO4. (All the tests were taken on carbon paper). -- Figure S21. (a) The LSV curves of carbon paper measured in 0.5 M H2SO4 toward HER at scan rate 10 mV/s. (b) The data of CoP-HS, CoP2-HS and CoP3-HS test in 0.5 M H2SO4. -- Figure S22. The CV curves of CoP-HS, CoP2-HS and CoP3-HS measured in 0.5 M H2SO4 solution for 1st (a), 3rd (b), 5th (c), and 10th (d) cycles at a scan rate 10 mV/s. -- Figure S23. (a) The chronopotentiometry curve of CoP at the current density of -20 mA cm-2 for 100 h in 0.5 M H2SO4. (b) The SEM image and EDS chemical mapping (d) of CoP single-shelled nanocages after 100 h HER stability measurement. -- Figure S24. Overall water splitting activities of CoP||CoP and Pt/C||IrO2. -- Figure S25. (a, b, c, d, e, f) Corresponding levels of oxygen and hydrogen gas generated at 0 s, 200 s, 400 s, 600 s, 800 s, 1000 s. -- Figure S26. Optimized configuration of CoP-HS adsorbed with H. -- Figure S27. Optimized configuration of CoP2-HS adsorbed with H. -- Figure S28. Optimized configurations of CoP3-HS adsorbed with H. -- Figure S29. HER free energy changes of CoP-HS, CoP2-HS and CoP3-HS at P-sites and Co-site. in 0.5 M H2SO4. (c) P(2p) XPS spectra of CoP-HS after 100 h HER stability. -- Figure S30. The normalized LSV curves of CoP-HS, CoP2-HS, and CoP3-HS. -- Figure S31. The correlation between the HER free energy changes based on Co-sites of CoPx-HS and the normalized overpotential as well as Tafel slope measurement. -- Table S1. Elemental composition of Co and P in the different cobalt phosphides. -- Table S2. Comparison of the alkaline OER efficiency of those cobalt phosphides with other reported advanced cathodic materials. -- Table S3. Comparison of the alkaline HER efficiency of this CoP with other reported advanced cathodic materials. -- Table S4. Comparison of the acidic HER efficiency of this CoP with other reported advanced cathodic materials. -- Table S5. Comparison of the alkaline overall water-splitting efficiency of this CoP with other reported advanced bifunctional catalysts., Peer reviewed