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Relació d'ajuts concedits a autors de la Universitat de Barcelona per publicar en accés obert durant l'any 2014. Inclou en format CSV el DOI, el títol, els autors, el nom de la revista i l'import concedit

Podeu consultar l'informe a que corresponen les dades a: http://hdl.handle.net/2445/100167

Dades primàries associades a l'article publicat a PLoS Neglected Tropical Diseases, vol. 10, num. 10, p. e0005009 [http://dx.doi.org/10.1371/journal.pntd.0005009], P. vivax infection during pregnancy has been associated with poor outcomes such as anemia, low birth weight and congenital malaria, thus representing an important global health problem. However, no vaccine is currently available for its prevention. Vir genes were the first putative virulent factors associated with P. vivax infections, yet very few studies have examined their potential role as targets of immunity. We investigated the immunogenic properties of five VIR proteins and two long synthetic peptides containing conserved VIR sequences (PvLP1 and PvLP2) in the context of the PregVax cohort study including women from five malaria endemic countries: Brazil, Colombia, Guatemala, India and Papua New Guinea (PNG) at different timepoints during and after pregnancy. Antibody responses against all antigens were detected in all populations, with PNG women presenting the highest levels overall. P. vivax infection at sample collection time was positively associated with antibody levels against PvLP1 (fold-increase: 1.60 at recruitment -first antenatal visit-) and PvLP2 (fold-increase: 1.63 at delivery), and P. falciparum co-infection was found to increase those responses (for PvLP1 at recruitment, fold-increase: 2.25). Levels of IgG against two VIR proteins at delivery were associated with higher birth weight (27 g increase per duplicating antibody levels, p<0.5). Peripheral blood mononuclear cells from PNG uninfected pregnant women had significantly higher antigen-specific IFN-g TH1 responses (p=0.006) and secreted less pro-inflammatory cytokines TNF and IL-6 after PvLP2 stimulation than P. vivax-infected women (p<0.5). These data demonstrate that VIR antigens induce the natural acquisition of antibody and T cell memory responses that might be important in immunity to P. vivax during pregnancy in very diverse geographical settings.

Dades primàries associades a un article publicat a la revista PeerJ disponible a l'adreça http://dx.doi.org/10.7717/peerj.2725, Podeu consultar l'article a: http://hdl.handle.net/2445/104686, Background. White adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defence, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT by breaking up the matrix of collagen fibres, however, it is unclear to what extent adipocyte number in primary cultures correlates with their number in intact WAT, since recovery and viability are often unknown. Experimental design. Epididymal WAT of 4-6 young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of lactate, glycerol and NEFA production. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were also measured. The presence of non-nucleated cells (erythrocytes) was also estimated. Results. Cell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70-75%. Adipocytes were 7%, erythrocytes 68% and other stromal (nucleated cells) 24% of total WAT cells. However, their overall volumes were, 91%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 2.5% of WAT. Conclusions. The methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have found, also, that the "live cell mass" of adipose tissue is very small (about 25 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood). This fact, translates (with respect to the actual "live cytoplasm" size) into an extremely high metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.

Dades primàries associades a l'article publicat a BMC Pediatrics, vol. 17 [https://doi.org/10.1186/s12887-017-0816-x], Background: Young children bear the world’s highest prevalence of anaemia, the majority of which is of multifactorial aetiology, which in turn hampers its successful prevention. Even moderate degrees of anaemia are associated with increased mortality and morbidity. Despite this evidence, there is a lack of effective preventive programs and absence of consensus in the safety of iron supplementation in malaria areas, which reflects the poor understanding of the contribution of different aetiologies to anaemia. In order to reduce the anaemia burden in the most vulnerable population, a study to determine the aetiology of anaemia among pre-school Mozambican children was performed. Methods: We undertook a case-control study of 443 preschool hospitalized children with anaemia (haemoglobin concentration <11g/dl) and 289 community controls without anaemia. Inclusion criteria were: age 1-59 months, no blood transfusion in the previous month, residence in the study area and signed informed consent. Both univariable and multivariable logistic regression analyses were performed to identify factors associated with anaemia and adjusted attributable fractions (AAF) were estimated when appropriate. Results: Malaria (adjusted odds ratio (AOR)=8.39, p<0.0001; AAF=37%), underweight (AOR=8.10, p<0.0001; AAF=43%), prealbumin deficiency (AOR=7.11, p<0.0001; AAF=77%), albumin deficiency (AOR=4.29, p=0.0012; AAF=30%), HIV (AOR=5.73, p=0.0060; AAF=18%), and iron deficiency (AOR=4.05, p<0.0001; AAF=53%) were associated with anaemia. Vitamin A deficiency and α-thalassaemia were frequent (69% and 64%, respectively in cases) but not independently related to anaemia. Bacteraemia (odds ratio (OR)=8.49, p=0.004), Parvovirus-B19 (OR=6.05, p=0.017) and Epstein-Barr virus (OR=2.10, p=0.0015) infections were related to anaemia only in the unadjusted analysis. Neither vitamin B12 deficiency nor intestinal parasites were associated with anaemia. Folate deficiency was not observed. Conclusions: Undernutrition, iron deficiency, malaria, and HIV are main factors related to anaemia in hospitalised Mozambican preschool children. Effective programs and strategies for the prevention and management of these conditions need to be reinforced. Specifically, prevention of iron deficiency that accounted in this study for more than half of anaemia cases would have a high impact in reducing the burden of anaemia in children living under similar conditions. However this deficiency, a common preventable and treatable condition, remains neglected by the international public health community.

Dades primàries associades a la publicació disponible a https://doi.org/10.1371/journal.pone.0178172, Podeu consultar l'article a: http://hdl.handle.net/2445/113259, Registre de ressonància magnètica funcional de subjectes diagnosticats de Diabetes Tipus I aparellats per edat i nivell educatiu amb un grups de controls. El registre de senyal cerebral està fet a partir de dues tasques vinculades amb llenguatge. Una d'elles amb components de working memory i l'altra no. Són dades analitzables amb eines específiques de R, MathLab, SMP o FSL.

Dades primàries associades a un article enviat a la revista PeerJ i pendent d'avaluació (maig 2017), Podeu consultar l'article a: http://hdl.handle.net/2445/115482, Background: Diet deeply affects the food selection and ingestion both in humans and rodents, often resulting in excess energy intake. Methods: We investigated this process comparing two different high-fat dietary approaches to induce obesity, in which all rats received about 40% of their energy intake as lipids. The main nutrient difference between the diets, when compared with controls fed standard lab chow, was the lipid content. Cafeteria diets (K) were devised to be tasty, and thus highly desirable to the rats, mainly for its diverse mix of tastes, particularly salty and sweet. This diet was compared with another high-fat (HF) potentially obesogenic diet, devised not to be as tasty as K, and prepared just supplementing standard chow pellets with fat. We also analysed the influence of sex on the effects of the diets. Results: K rats grew faster, especially the males, although females showed a higher proportion of body lipid, because of a high lipid, sugar and protein intake. HF weight change rates were not different from those of controls. In addition to high sugar, K rats also ingested large amounts of salt. With this study we have shown that the key factor eliciting the excess energy intake in a high-energy diet rat model was not solely or mainly their fat intake. The changes in body fat accrual were more a consequence of their appetence for the food. Conclusions: The results show that the significant presence of sugar and salt is a powerful factor promoting excess food intake, more effective than increasing diet lipid content. These effects were already observed after a relatively short treatment, additionally confirming the differential effects of sex on the hedonic and obesogenic response to diet.

Dades primàries associades l'article publicat a PLoS Neglected Tropical Diseases, vol. 11, num. 6, p. e0005606 [https://doi.org/10.1371/journal.pntd.0005606], P. vivax is a major parasite responsible for malaria in humans. While it is well known that pregnant women have an increased risk of P. falciparum infection and disease, less is known on the epidemiology and the impact of P. vivax in pregnancy. We conducted a health-facility based observational study in pregnant women from five endemic countries aimed to determine the burden of P. vivax infection in pregnancy and its impact on the mother and the newborn. We found that the prevalence of P. vivax malaria in pregnant women was overall low across sites, however submicroscopic infections were significantly higher in some areas. Pregnant women with clinical malaria experienced an increased risk of anaemia, which may have an impact on infant health. The results of this study can be useful to guide maternal health programs in settings where vivax malaria is endemic, and they highlight the importance to address a special population such as pregnant women while embracing malaria elimination in endemic countries.

Dades primàries associades a un article publicat a la revista PLoS ONE http://doi.org/10.1371/journal.pone.0184698, Podeu consultar l'article a: http://hdl.handle.net/2445/119911, Dades obtingudes en assajos on es presenta una pista audiovisual associada a un esdeveniment futur ('target') que apareix a la dreta o a l'esquerra de la pantalla, després d'un interval de temps curt o llarg, segons la pista. Els assajos estan codificats de tal manera que es pot saber què ha vist cada particpant en cada un dels assajos (pista E= Elmo / pista G = Big Birg; S = Slow (4 sec interval) / F = Fast (2 sec interval); R = Right (right location) / L = Left (left location). Per tots els participants es va associaciar E/S i G/F. L'associació d'espai (R-L) i temps (S-F) es va contrabalancejar entre els participants. Dades generades amb raw data eye tracking system (Tobii). Els arxius contenen informació de l'edat dels participants per poder separar els dos grups (12 mesos i 15 mesos), codi del participant i una inicial (per a comprovacions internes).

Relació d'ajuts concedits a autors de la Universitat de Barcelona per publicar en accés obert durant l'any 2016. Inclou en format CSV el DOI, el títol, els autors, el nom de la revista i l'import concedit