BUSCADOR RECOLECTA

Additional structural (XRD and STEM) and chemical x-ray microanalysis, a detailed description of the STEM image analysis and calculation of polarization images, including the LSAT substrate, and further details on the experimental conditions are provided in the supplementary material., Peer reviewed

File 1 (.doc): 1Table - Mean (±SD) δ13C and δ15N values (‰) of the prey species used to analyze the diet of the common bottlenose dolphin in Madeiran waters, per season; 1 figure - Simulated mixing region calculated for each season (‘summer/autumn’- top; ‘winter/spring’- bottom) with the most likely prey (white crosses) based on bi-plot interpretation.-- File 2 (.xls), Supplementary material of the article Trophic ecology of common bottlenose dolphins in a pelagic insular environment inferred by stable isotopes, https://doi.org/10.1007/s10750-023-05294-4, Peer reviewed

The dataset includes: Microstructure profiles collected with a MSS Sea&Sun profiler during the three intensive samplings of the cruise (I01, I02, I03), Ocean currents measured with a bottom moored RD Instruments acoustic Doppler profiler (ADCP, 300Khz) for the duration of the cruise, Acoustic backscatter from a ship-borne echosounder Simrad EK80 for the frequencies 18, 38, 70, 120 and 200 KHz and the three intensive samplings of the cruise (I01, I02, I03), European anchovy (Engraulis encrasicolus) egg counts from plankton hauls samplings, This dataset includes data collected during the cruise REMEDIOS-TL in the Ría de Pontevedra (NW Iberia) at station P2 (42.357°N, 8.773°W) from 29 June to 18 July 2018 onboard of the Research Vessel Ramón Margalef belonging to the Spanish Institude of Oceanography. The archived data are described in a manuscript entitled "Intense upper ocean mixing due to large aggregations of spawning fish" by Fernández Castro et al. published in Nature Geoscience: Fernández Castro, B., Peña, M., Nogueira, E. et al. Intense upper ocean mixing due to large aggregations of spawning fish. Nat. Geosci. 15, 287–292 (2022). https://doi.org/10.1038/s41561-022-00916-3. The manuscript presents evidence that night-time aggregations of anchovies produce intense ocean turbulence and mixing. All the data needed to support the conclusions of the article are included in this dataset, The REMEDIOS project is funded by the Spanish Ministry of Economy and Innovation under the research project REMEDIOS (CTM2016-75451-C2-1-R), acoustic_backscatter_EK80_REMEDIOS_TL_I01.nc.-- acoustic_backscatter_EK80_REMEDIOS_TL_I02.nc.-- acoustic_backscatter_EK80_REMEDIOS_TL_I03.nc.-- bottom_moored_ADCP_REMEDIOS_TL.nc.-- egg_counts.xls.-- MSS_microstructure_REMEDIOS_TL_I01.nc.-- MSS_microstructure_REMEDIOS_TL_I02.nc.-- MSS_microstructure_REMEDIOS_TL_I03.nc, Peer reviewed

The N-terminal tails of eukaryotic histones are frequently posttranslationally modified. The role of these modifications in transcriptional regulation is well-documented. However, the extent to which the enzymatic processes of histone posttranslational modification might affect metabolic regulation is less clear. Here, we investigated how histone methylation might affect metabolism using metabolomics, proteomics, and RNA-seq data from cancer cell lines, primary tumour samples and healthy tissue samples. In cancer, the expression of histone methyltransferases (HMTs) was inversely correlated to the activity of NNMT, an enzyme previously characterised as a methyl sink that disposes of excess methyl groups carried by the universal methyl donor S-adenosyl methionine (SAM or AdoMet). In healthy tissues, histone methylation was inversely correlated to the levels of an alternative methyl sink, PEMT. These associations affected the levels of multiple histone marks on chromatin genome-wide but had no detectable impact on transcriptional regulation. We show that HMTs with a variety of different associations to transcription are co-regulated by the Retinoblastoma (Rb) tumour suppressor in human cells. Rb-mutant cancers show increased total HMT activity and down-regulation of NNMT. Together, our results suggest that the total activity of HMTs affects SAM metabolism, independent of transcriptional regulation., Peer reviewed

GTEx NNMT-HMT correlation all tissues full labelled plots., Peer reviewed

(A) Schematic drawings of an S9 egg chamber illustrating the mirrorGal4 pattern of expression (mirrGal4, pink) and the point of ablation in the basal side of FCs (blue bar). NCs are in gray, BCs in yellow, FCs in purple, and BM in blue. (B) Images of life S9 control mirrGal4 and mirr>AbiRNAi egg chambers expressing Resille-GFP before and after FCs bonds are ablated. Blue bars indicate points of ablation. (C) Quantification of initial velocity of vertex displacement of the indicated ablated bonds. (D) Schematic drawing of an S9 egg chamber illustrating the mirrGal4 pattern of expression (pink) and the point of ablation in the NCs (blue bar). (E) Images of life S9 egg chambers of the indicated genotypes before and after NC bonds are ablated. Blue bars indicate points of ablation. (F) Quantification of initial velocity of vertex displacement of the indicated ablated bonds. (G–H’) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. Discontinuous yellow lines demarcate the region between the anterior border of the egg chamber and the oocyte anterior membrane. (I, J) Quantification of BC migration speed in the area anterior to (I) and at the (J) mirr expressing region. The statistical significance of differences was assessed with a t test, * P value < 0.05, ** P value < 0.01, and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bar in B, E, and G–H’, 5 μm, 10 μm, and 20 μm, respectively. The raw data underlying panels C, F, I, and J are available in S1 Data. BC, border cell; BM, basement membrane; FC, follicle cell; GFP, green fluorescent protein; NC, nurse cell., Peer reviewed

The basement membrane (BM) is a specialized extracellular matrix (ECM), which underlies or encases developing tissues. Mechanical properties of encasing BMs have been shown to profoundly influence the shaping of associated tissues. Here, we use the migration of the border cells (BCs) of the Drosophila egg chamber to unravel a new role of encasing BMs in cell migration. BCs move between a group of cells, the nurse cells (NCs), that are enclosed by a monolayer of follicle cells (FCs), which is, in turn, surrounded by a BM, the follicle BM. We show that increasing or reducing the stiffness of the follicle BM, by altering laminins or type IV collagen levels, conversely affects BC migration speed and alters migration mode and dynamics. Follicle BM stiffness also controls pairwise NC and FC cortical tension. We propose that constraints imposed by the follicle BM influence NC and FC cortical tension, which, in turn, regulate BC migration. Encasing BMs emerge as key players in the regulation of collective cell migration during morphogenesis., Peer reviewed

[ES] El conjunto de datos está dividido en tres subcarpetas, dentro de las cuales los datos se encuentran agrupados por especies: Eggs: Proporciona más de 900 imágenes en formato RAW en el rango visual del espectro visible de puestas completas sobre fondo negro, correspondientes a 14 especies distintas. Nests: Proporciona más de 800 imágenes de nidos de 6 especies distintas, tomadas en su entorno desde 1 metro de distancia y a 70 cm de altura. En este segundo conjunto de imágenes, se incluyen imágenes de los nidos tanto en el rango visible como en ultravioleta cercano. Incubating Behavior: Proporciona más de 950,000 imágenes en formato jpg correspondientes a secuencias de vigilancia del comportamiento de incubación de 5 especies distintas, junto con información de las condiciones ambientales., [EN] The dataset is divided into three subfolders, within which the data is grouped by species: Eggs: Provides over 900 images in RAW format within the visible spectrum of complete clutches on a black background, corresponding to 14 different species. Nests: Provides over 800 images of nests from 6 different species, taken in their environment from 1 meter away and at a height of 70 cm. This set includes images of the nests in both the visible range and near-ultraviolet. Incubating Behavior: Provides over 950,000 jpg images corresponding to surveillance sequences of incubation behavior from 5 different species, along with information on environmental conditions., [ES] Este conjunto de datos, generado en el marco del proyecto CAMNID: CGL2017-83518-P, Plan Nacional I+D+i, contiene información visual sobre huevos, nidos y comportamiento durante la incubación de diferentes especies de aves limícolas (shorebirds). Permite la realización de análisis de coloración y moteado, entre otros, para las especies en estudio. Asimismo, se proporcionan secuencias de imágenes de monitorización de diversos nidos de estas especies a lo largo de 3 horas (entre las 12:00 y las 15:00) junto con las condiciones ambientales durante la vigilancia. Esto permite la realización de estudios de los comportamientos de estas aves durante la incubación, abriendo la posibilidad de estudiar diversos mecanismos de mitigación de estrés térmico o las alternancias parentales macho/hembra durante la incubación en función de las condiciones ambientales, [EN] This dataset, generated within the framework of the CAMNID project: CGL2017-83518-P, Plan Nacional I+D+i, contains visual information on eggs, nests, and incubation behavior of various shorebird species. It enables color and spotting pattern analyses, among other studies, for the species included. Additionally, it provides image sequences monitoring various nests of these species over a 3-hour period (from 12:00 to 15:00) along with the environmental conditions during the surveillance. This allows for studies of the birds' behavior during incubation, opening the possibility to study mechanisms of thermal stress mitigation and male/female parental alternation during incubation based on environmental conditions., CAMNID PROJECT: Grant: CGL2017-83518-P, Plan Nacional I+D+i: EFECTOS DE LAS INVASIONES GEOLOGICAS DE ORIGEN ANTROPICO SOBRE LAS ESTRATEGIAS DE CAMUFLAJE DE NIDOS EN EL CHORLITEJO PATINEGRO CHARADRIUS ALEXANDRINUS, The dataset is comprissed of three folders. Eggs, Nests, and BehavioralMonitoring. ** Folder: Eggs: Photographs of shorebird eggs from a zenital view in raw format. - INFORMATION_TABLE.xlsx: Excel file with information about each picture in the Eggs folder subset. Image name can be used as unique identifier. - Folders corresponding to eggs of 14 different species. Species name corresponding to folder name. - Inside each folder, a single RAW format image file for each egg is provided, ** Folder: Nests: Zenital pictures of shorebirds nests in natural environments both in the Vis and UV wavelength ranges. - INFORMATION_TABLE.xlsx: Excel file with information about each picture in the Nests folder subset. Image name can be used as unique identifier. - Folders corresponding to 6 different species. Species name corresponding to folder name. - Inside each folder, two images per nest are provided. One for the visible wavelength range ({ImageName}_VIS.ARW) and another one for the UV range ({ImageName}_UV.ARW), **Folder: BehavioralMonitoring: Pictures showing the incubating activity of shorebird species. Additionally, environmental conditions are also provided. - README.txt: Readme file providing the description of each folder in the dataset - Folders corresponding to 5 shorebirds species being monitored. - Inside each species folder: ---subfolder: DATE_OF_OBSERVATION: | |---subfolder: DATALOGGERCODE (DTXX (XX from 0 to 9)) | |____FRAME_XXX_STD.jpg: XXXth Picture taken under normal conditions | |____YYY_ACT.jpg: YYYth Picture corresponding to a special activitity condition | |____DATA_LOGGER.txt: csv file containing enviromental conditions during this experiment. Updated every 100s | |____DTXX.gpx: GPS position of this observation (gpx standard format) | |____DTXX_GPS.txt: GPS position for this observation in txt format (the gpx file is not always available), Peer reviewed

The origin of the FtsZ/tubulin protein family was extremely relevant for life since these proteins are present in nearly all organisms, carrying out essential functions such as cell division or forming a major part of the cytoskeleton in eukaryotes. Therefore, investigating the early evolution of the FtsZ/tubulin protein family could reveal crucial aspects of the diversification of the three domains of life. In this study, we revisited the phylogenies of the FtsZ/tubulin protein family in an extensive prokaryotic diversity, focusing on the main evolutionary events that occurred during its evolution. We found evidence of its early origin in the last universal common ancestor since FtsZ was present in the last common ancestor of Bacteria and Archaea. In bacteria, ftsZ genes are genomically associated with the bacterial division gene cluster, while in archaea, ftsZ duplicated prior to archaeal diversification, and one of the copies is associated with protein biosynthesis genes. Archaea have expanded the FtsZ/tubulin protein family with sequences closely related to eukaryotic tubulins. In addition, we report novel CetZ-like groups in Halobacterota and Asgardarchaeota. Investigating the C-termini of prokaryotic paralogs basal to eukaryotic tubulins, we show that archaeal CetZ, as well as the plasmidic TubZ from Firmicutes, most likely originated from archaeal FtsZ. Finally, prokaryotic tubulins are restricted to Odinarchaeaota and Prosthecobacter species, and they seem to belong to different molecular systems. However, their phylogenies suggest that they are closely related to α/β-tubulins pointing to a potential ancestrality of these eukaryotic paralogs of tubulins, Peer reviewed

able S1: Species, sample, coverage, non-conversion rate (%), and average DNA methylation levels (mCG/CG) for all datasets generated in this study. Table S2: Genomic location of all differentially methylated regions (DMRs) identified in the medaka genome. Table S3: DMR-linked genes in zebrafish and medaka genomes., Peer reviewed