BUSCADOR RECOLECTA

Supporting Information includes: Supporting Methods.-- Supporting Results.-- Figures S1 to S7.-- Tables S1, S2.-- SI References, Copyright © 2023 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND)., Peer reviewed

[Description of methods used for collection/generation of data] Data were acquired through an RGB camera mounted on a UAV at an altitude of 11m. From the images obtained by the UAV, geomatic products were systematically generated for the creation of orthomosaics. This task was performed by incorporating information extracted from the RGB channels using Agisoft PhotoScan software (Agisoft LLC, St. Louis, MO). The resulting orthomosaics were subdivided into smaller sections. To identify and label weed species, malherbology experts carried out the task manually. This involved drawing bounding boxes around each plant and annotating various visible objects present in each divided image. This process was executed using the graphical tool labelImg open source software. The final result consists of a collection of individual images, each corresponding to a specific label, i.e., to each plant of each identified species., The dataset, titled 'Unmanned Aerial Vehicle Imagery for Early Stage Weed Classification and Detection in Maize and Tomato Crops,' comprises RGB images captured from an unmanned aerial vehicle flying at an altitude of 11 meters. These images were taken during the early growing season of two summer crops: Maize (BBCH14) at the CSIC experimental farm La Poveda in Arganda del Rey, Madrid, Spain, and Tomato (BBCH501) in commercial plots located in Santa Amalia, Badajoz, Spain. The dataset includes 33,467 labels representing weeds (Atriplex patula, Chenopodium album, Convolvulus arvensis, Cyperus rotundus, Lolium rigidum, Portulaca oleracea, Salsola kali, Solanum nigrum) and crops (maize, tomato). All images are saved in *.jpg format and have been labeled using partitions extracted from an orthomosaic. The final result consists of a collection of individual images, each corresponding to a specific label, i.e., to each plant of each identified species., This work was supported by the Spanish Research State Agency (AEI) through the Project PID2020-113229RB-C41/AEI/10.13039/501100011033. The lead author, G.A. Mesías-Ruiz has been a beneficiary of a FPI fellowship by the Spanish Ministry of Education and Professional Training (PRE2018-083227)., Peer reviewed

S1 Video. Correlation between cibarial pump movement and related EPG waveform (Xi). (MP4), The meadow spittlebug Philaenus spumarius plays a key role in the transmission of the bacterium Xylella fastidiosa to olive in Apulia (South Italy). Currently, available data on P. spumarius feeding behavior is limited, and a real-time observation of the different steps involved in stylet insertion, exploratory probes, and ingestion, has never been carried out. Therefore, we performed an EPG-assisted characterization of P. spumarius female feeding behavior on olive, in order to detect and analyze the main EPG waveforms describing their amplitude, frequency, voltage level, and electrical origin of the traces during stylet penetration in plant tissues. Thereafter, each of the main waveforms was correlated with specific biological activities, through video recording and analysis of excretion by adults and excretion/secretion by nymphs. Furthermore, the specific stylet tips position within the plant tissues during each of the waveforms observed was assessed by microcomputer tomography (micro-CT). Additional EPG-recordings were carried out with males of P. spumarius on olive, in order to assess possible sex-related differences. P. spumarius feeding behavior can be described by five main distinct waveforms: C (pathway), Xc (xylem contact/pre-ingestion), Xi (xylem sap ingestion), R (resting), N (interruption within xylem phase). Compared to males, females require shorter time to begin the first probe, and their Xi phase is significantly longer. Furthermore, considering the single waveform events, males on olive exhibit longer np and R compared to females., Peer reviewed

[EN] The electrochemical behaviour of each layer deposited on CoCr surfaces was examined by electrochemical impedance spectroscopy (EIS) for 7 days in 3 g/L hyaluronic acid (HA) aqueous solution. Impedance tests were performed in a three-electrode electrochemical cell with the modified CoCr surfaces acting as the working electrode, a Pt wire acting as counter electrode, and Ag/AgCl (3 M KCl) as a reference electrode. The testing area of the working electrode for all electrochemical tests was 0.39 cm2. An Autolab32 potentiostat/galvanostat was used to generate a sinusoidal wave of 10 mV amplitude in the frequency range 100 kHz to 1 mHz, logarithmically spaced by 5 points/decade, which was applied at the corrosion potential Ecorr. Nova software was used for the data acquisition. All the tests were performed in duplicate. The dataset includes only the experimental data used in the paper. The following files for each surface modification and each immersion time are included: CoCr, CoCr-OH, CoCr-OH-Si45, CoCr-OH-Si45-GO12h, CoCr-OH-Si45-GO24h, CoCr-OH-Si75, CoCr-OH-Si75-GO12h, CoCr-OH-Si75-GO24h. Each file is composed of 5 columns: frequency, real impedance (Z'), imaginary impedance (Z''), Impedance modulus (Z) and phase angle (Phase). All files are in open extension such as .txt and are protected by a Creative Commons Attribution 4.0 International (CC BY 4.0) licence. [ES] El comportamiento electroquímico de cada capa depositada en superficies de CoCr fue examinado mediante espectroscopía de impedancia electroquímica (EIS) durante 7 días sumergidas en una solución acuosa de ácido hialurónico (HA) al 3 g/L. Las pruebas de impedancia se realizaron en una celda electroquímica de tres electrodos con las superficies modificadas de CoCr actuando como electrodo de trabajo, un alambre de Pt actuando como electrodo auxiliar y Ag/AgCl (3 M KCl) como electrodo de referencia. El área de ensayo del electrodo de trabajo para todas las pruebas electroquímicas fue de 0.39 cm2. Se utilizó un potenciostato/galvanostato Autolab32 para generar una onda sinusoidal de amplitud de 10 mV en el rango de frecuencia de 100 kHz a 1 mHz, espaciada logarítmicamente por 5 puntos/década, que se aplicó en el potencial de corrosión Ecorr. Se utilizó el software Nova para la adquisición de datos. Todas las pruebas se realizaron por duplicado. El conjunto de datos incluye solo los datos experimentales utilizados en el artículo. Los siguientes archivos para cada modificación de superficie y cada tiempo de inmersión están incluidos: CoCr, CoCr-OH, CoCr-OH-Si45, CoCr-OH-Si45-GO12h, CoCr-OH-Si45-GO24h, CoCr-OH-Si75, CoCr-OH-Si75-GO12h, CoCr-OH-Si75-GO24h. Cada archivo está compuesto por 5 columnas: frecuencia, impedancia real (Z'), impedancia imaginaria (Z''), módulo de impedancia (Z) y ángulo de fase (Fase). Todos los archivos están en extensión abierta como .txt y están protegidos por una licencia Creative Commons Attribution 4.0 International (CC BY 4.0)., [EN] This is the experimental dataset used in the paper “Barrier Graphene Oxide on a CoCr Alloy via Silane/GO Covalent Bonding and Its Electrochemical Behavior in a Simulated Synovial Fluid Electrolyte". The article includes the experimental procedure carried out for the deposition of graphene oxide on CoCr surfaces: CoCr was hydroxylated through alkalization in NaOH 5 M for 2 h, (sample named CoCr-OH). Subsequently, silanization was carried out using APTES. APTES was pre-mixed at 2% vol in isopropanol-water (200:1 v/v) and stirred for 1 h. After this, CoCr-OH samples were immersed in the APTES solution at room temperature for 1 min. The silane-coated samples were kept under curing conditions at 45 °C (CoCr-OH-Si45 sample) and 75 °C (CoCr-OH-Si75 sample) for 24 h and 30 min, respectively. Finally, CoCr-OH-Si45 and CoCr-OH-Si75 surfaces were immersed in 4 g/L GO aqueous suspension at 60 °C for 12 h and 24 h to obtain CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h and CoCr-OH-Si45-GO24h, and CoCr-OH-Si75-GO24h. This data set includes the results of the electrochemical impedance data obtained for each CoCr surface: CoCr (CoCr, CoCr-OH, CoCr-OHSi45, CoCr-OH-Si75 CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h, immersed in 3 g/L Hyaluronic acid solution for 7 days., [ES] Este es el conjunto de datos experimentales utilizado en el artículo "Barrera de Óxido de Grafeno en una Aleación CoCr mediante enlace covalente de Silano/GO y su comportamiento electroquímico en un electrolito de fluido sinovial simulado". El artículo incluye el procedimiento experimental llevado a cabo para la deposición de óxido de grafeno sobre superficies de CoCr: CoCr fue hidroxilado mediante alcalinización en NaOH 5 M durante 2 horas (muestra denominada CoCr-OH). Posteriormente, se llevó a cabo la silanización utilizando APTES. APTES fue premezclado al 2% en volumen en isopropanol-agua (200:1 v/v) y agitado durante 1 hora. Después, las muestras de CoCr-OH fueron sumergidas en la solución de APTES a temperatura ambiente durante 1 minuto. Las muestras recubiertas con silano se mantuvieron bajo condiciones de curado a 45 °C (muestra CoCr-OH-Si45) y 75 °C (muestra CoCr-OH-Si75) durante 24 horas y 30 minutos, respectivamente. Finalmente, las superficies CoCr-OH-Si45 y CoCr-OH-Si75 fueron sumergidas en suspensión acuosa de 4 g/L GO a 60 °C durante 12 y 24 horas obteniendo las superficies CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h. Este conjunto de datos incluye los resultados de los datos de impedancia electroquímica obtenidos para cada superficie de CoCr (CoCr, CoCr-OH, CoCr-OHSi45, CoCr-OH-Si75 CoCr-OH-Si45-GO12h, CoCr-OH-Si75-GO12h y CoCr-OH-Si45-GO24h, y CoCr-OH-Si75-GO24h sumergidas en una solución de 3 g/L ácido hialurónico durante 7 días., We acknowledge financial support from the Spanish Ministerio de Ciencia, Innovación y Univeesidades, “ERDF A way of making Europe” and “ESF Investing in your future” through projects RTI2018-101506-B-C31 and RTI2018-101506-B-C33, and PRE2019-090122., Peer reviewed

S2 Video. Video obtained from micro-CT showing P. spumarius stylets in xylem vessel. (MKV), The meadow spittlebug Philaenus spumarius plays a key role in the transmission of the bacterium Xylella fastidiosa to olive in Apulia (South Italy). Currently, available data on P. spumarius feeding behavior is limited, and a real-time observation of the different steps involved in stylet insertion, exploratory probes, and ingestion, has never been carried out. Therefore, we performed an EPG-assisted characterization of P. spumarius female feeding behavior on olive, in order to detect and analyze the main EPG waveforms describing their amplitude, frequency, voltage level, and electrical origin of the traces during stylet penetration in plant tissues. Thereafter, each of the main waveforms was correlated with specific biological activities, through video recording and analysis of excretion by adults and excretion/secretion by nymphs. Furthermore, the specific stylet tips position within the plant tissues during each of the waveforms observed was assessed by microcomputer tomography (micro-CT). Additional EPG-recordings were carried out with males of P. spumarius on olive, in order to assess possible sex-related differences. P. spumarius feeding behavior can be described by five main distinct waveforms: C (pathway), Xc (xylem contact/pre-ingestion), Xi (xylem sap ingestion), R (resting), N (interruption within xylem phase). Compared to males, females require shorter time to begin the first probe, and their Xi phase is significantly longer. Furthermore, considering the single waveform events, males on olive exhibit longer np and R compared to females., Peer reviewed

This study presents an exceptional collection of 54 Late Pleistocene human remains that correspond to at least three Neanderthal individuals from Simanya Gran, the main gallery of Cova Simanya, located in the northeastern Iberian Peninsula. The collection comprised 53 unpublished remains that were unearthed during the 1970s and an additional tooth discovered during 2021 excavations. The specimens represent an adult with a small stature, a periadolescent aged approximately 11.5 years, and an immature individual aged approximately 7.7 years, thus offering a more complete demographic perspective. The collection encompasses diverse anatomical parts including upper and lower dentition, mandible, vertebrae, and limb bones from both the upper and lower extremities. Attempts to extract aDNA were unsuccessful. Renewed archaeological investigations at Cova Simanya have facilitated the reevaluation of the original stratigraphic context of these remains, leading to the discovery of the additional tooth, aligning with the periadolescent individual. This assemblage is currently the most extensive Neanderthal collection from the northeastern Mediterranean Iberia, offering invaluable insights into the morphology and evolutionary trajectory of Late Pleistocene hominins. Hence, Simanya Neanderthals will enhance our understanding of Neanderthal demographics and evolution, paving the way for an in-depth examination of the morphological diversity and evolutionary context of Iberian Neanderthals., Peer reviewed

© 2023 The Authors. Global Change Biology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited., This supplementary appendix contains: 1. Supplementary Methods.-- 2. Supplementary Figures.-- 3. Supplementary References, Peer reviewed

Winter mortality and weight loss. File name: ‘raw_data_weights’ CSV file providing pre-wintering weight, percent weight loss and emergence success of O. cornuta females exposed to three simulated wintering treatments. Variables cc_treatment = wintering treatments (C = Current; NF = Near-future; DF = Distant-future) pre_wint_weight_mg = pre-wintering weight in mg weight_loss_per = proportion of body weight loss emergence_succ = 1 (success) or 0 (failure), Phototaxis test. File name: ‘raw_data_phototaxis’ CSV file providing the results of the Phototaxis test for O. cornuta females exposed to three wintering treatments and three doses of the insecticide sulfoxaflor. Variables cc_treatment = wintering treatments (C = Current; NF = Near-future; DF = Distant-future) pest_treatment = pesticide treatments (CONTROL = 0 dose; LD = Low dose; HD = High dose) phototaxis = 1 (i.e. postive, the bee walked to the light source within 60 seconds) or 0 (i.e. negative, the bee walked but did not reach the light source)., Syrup consumption and survival. File name: ‘raw_data_longevity’ CSV files providing the daily syrup consumption and longevity of O. cornuta females exposed to three wintering treatments and three doses of the insecticide sulfoxaflor. Variables cc_treatment = wintering treatments (C = Current; NF = Near-future; DF = Distant-future) pest_treatment = pesticide treatments (CONTROL = 0 dose; LD = Low dose; HD = High dose) longevity = bee longevity (days) event = column required for synergism analysis daily_consumption_ul = daily consumption of the feeding solution (ul), Synergism analysis File name: 'synergism_analysis.R' (R file) Description: Full R script for the analysis of synergism between climate change and insecticide exposure on survival., Bee populations are exposed to multiple stressors, including land-use change, biological invasions, climate change and pesticide exposure, that may interact synergistically. We analyze the combined effects of climate warming and sublethal insecticide exposure in the solitary bee Osmia cornuta. Previous Osmia studies show that warm wintering temperatures cause body weight loss, lipid consumption and fat body depletion. Because the fat body plays a key role in xenobiotic detoxification, we expected that bees exposed to climate warming scenarios would be more sensitive to pesticides. We exposed O. cornuta females to three wintering treatments: current scenario (2007-2012 temperatures), near-future (2021-2050 projected temperatures), and distant-future (2051-2080). Upon emergence in spring, bees were orally exposed to three sublethal doses of an insecticide (Closer, a.i. sulfoxaflor; 0, 4.55 and 11.64 ng a.i./bee). We measured the combined effects of wintering and insecticide exposure on phototactic response, syrup consumption, and longevity. Wintering treatment by itself did not affect winter mortality, but body weight loss increased with increasing wintering temperatures. Similarly, wintering treatment by itself hardly influenced phototactic response or syrup consumption. However, bees wintered at the warmest temperatures had shorter longevity, a strong fecundity predictor in Osmia. Insecticide exposure, especially at the high dose, impaired the ability of bees to respond to light, and resulted in reduced syrup consumption and longevity. The combination of the warmest winter and the high insecticide dose resulted in a 70% longevity decrease. Smaller bees, resulting from smaller pollen-nectar provisions, had shorter longevity suggesting nutritional stress may further compromise fecundity in O. cornuta. Our results show a synergistic interaction between two major drivers of bee declines, and indicate that bees will become more sensitive to pesticides under the current global warming scenario. Our findings have important implications for pesticide regulation and underscore the need to consider multiple stressors to understand bee declines., Funding: Ministerio de Ciencia e Innovación y Ministerio de Universidades., Peer reviewed

Table A.1. Daily syrup consumption (µl day−1) in Osmia bicornis females orally exposed to various pesticide treatments. CTRL: chronic exposure to regular syrup; FUNG: chronic exposure to regular syrup with a pulse of tebuconazole (185 mg L-1) on day 3; INS: chronic exposure to syrup with imidacloprid (5 µg L-1); MIX: chronic exposure to syrup with imidacloprid (5 µg L-1) + a pulse of tebuconazole (185 mg L-1) on day 3. Pre-pulse: first 2 days of exposure; Pulse: day 3; Post-pulse: after the 3rd up to the date of 50% mortality within each treatment; TOTAL: Daily syrup consumption from the beginning of exposure until the 50% mortality within each treatment.-- Table A.2. Results of Mann-Whitney U Test (One-sided tests*) when syrup consumption is compared between pre- pulse and post pulse- time.-- Table A.3. Results of the Kernel regression analysis (dependent variable is daily syrup consumption).-- Table A.4. Results of Fleming-Harrington test for equality of survivor functions (middle differences).-- Table A.5. Results of Cox regression model., © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/., Peer reviewed

[EN] CoCrGO and CoCrGOHA surfaces were characterized by Raman spectroscopy and electrochemical impedance spectroscopy (EIS). Raman spectra were collected with a Raman spectrometer B&W TEK (BTC675N) using a 532 nm wavelength laser. Exposure time was set at 100 ms per scan. Spectral data were processed using BWSpec4 software with dark-frame substraction. Spectra were collected from 50 cm-1 to 4000 cm-1 with integration times of 100 s for CoCrGO and CoCrGOH surfaces and 300 s for graphene oxide (GO) reference sample and 20 s for hyaluronic acid (HA) reference sample. EIS data for CoCrGOHA surfaces were recorded up to 23 days of immersion in 3 g/L HA aqueous electrolyte. A three-electrode electrochemical cell consisting of CoCrGO or CoCrGOHA surfaces as working electrodes, a Pt wire as counter electrode and Ag/AgCl (3 M KCl) as reference electrode were used. The testing area of the working electrode for all electrochemical tests was 0.39 cm2. An Autolab32 potentiostat/galvanostat was used to generate a sinusoidal wave of 10 mV amplitude applied at the corrosion potential Ecorr in the 100 kHz- 1 mHz frequency range logarithmically spaced by 5 points/decade. Nova software was used for the data acquisition. All the tests were performed in duplicate. The dataset includes only the experimental data used in the paper. The following folders are included: RamanCoCrGO+CoCrGOHA and EISCoCrGO+CoCrGOHA. Inside Raman spectra folder, Raman files corresponding to CoCrGO and CoCrGOHA surfaces and GO and HA as reference are added. Each Raman file is composed of 8 columns, corresponding to pixel, wavelength, wavenumber, Ramanshift, Reference, Rawdata and Dark substracted. Each file of EIS data is composed of 5 columns: frequency, real impedance (Z’), imaginary impedance (imag Z), Impedance modulus (Z) and phase angle (Phase). All files are in open extension such as .txt and are protected by a Creative Commons Attribution 4.0 International (CC BY 4.0) licence. [ES] Las superficies de CoCrGO y CoCrGOHA fueron caracterizadas mediante espectroscopía Raman y espectroscopía de impedancia electroquímica (EIS). Los espectros Raman fueron recogidos con un espectrómetro Raman B&W TEK (BTC675N) utilizando un láser de longitud de onda de 532 nm. El tiempo de exposición se estableció en 100 ms para cada barrido. Los datos espectrales fueron procesados utilizando el software BWSpec4 con substracción de marco oscuro. Los espectros fueron registrados desde 50 cm-1 hasta 4000 cm-1 con tiempos de integración de 100 s para las superficies de CoCrGO y CoCrGOHA, y de 300 s para la muestra de referencia de GO y de 20 s para la muestra referencia de ácido hialurónico (HA). Los datos de EIS para las superficies CoCrGOHA fueron registrados hasta 23 días de inmersión en electrolito acuoso de 3 g/L HA. Se utilizó una celda electroquímica de tres electrodos consistente en las superficies CoCrGO o CoCrGOHA como electrodos de trabajo, un alambre de Pt como electrodo auxiliar y Ag/AgCl (3 M KCl) como electrodo de referencia. El área de ensayo del electrodo de trabajo para todas las pruebas electroquímicas fue de 0.39 cm2. Se utilizó un potenciostato/galvanostato Autolab32 para generar una onda sinusoidal de amplitud de 10 mV aplicada sobre el potencial de corrosión Ecorr en el rango de frecuencia de 100 kHz a 1 mHz espaciado logarítmicamente 5 puntos/década. Se utilizó el software Nova para la adquisición de datos. Todas las pruebas se realizaron por duplicado. El conjunto de datos incluye únicamente los datos experimentales utilizados en el artículo. Las siguientes carpetas están incluidas: RamanCoCrGO+CoCrGOHA y EISCoCrGO+CoCrGOHA. Dentro de la carpeta de espectros Raman, se agregan archivos Raman correspondientes a las superficies CoCrGO y CoCrGOHA, y las referencias de GO y HA. Dentro de la carpeta de impedancia (EIS) se han añadido los archivos de las superficies de CoCrGO y CoCrGOHA. Cada archivo Raman está compuesto por 8 columnas, correspondientes a píxel, longitud de onda, número de onda, desplazamiento Raman, referencia, datos en crudo y sustracción de fondo. Cada archivo de EIS está compuesto por 5 columnas: frecuencia, impedancia real (Z’), impedancia imaginaria (imag Z), módulo de impedancia (Z) y ángulo de fase (Fase). Todos los archivos están en formato de texto (.txt) y están protegidos por una licencia Creative Commons Attribution 4.0 International (CC BY 4.0)., [EN] This is the experimental dataset used in the paper “Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment". The article includes the experimental procedure carried out for the deposition of graphene oxide and posterior immobilization of hyaluronic acid on CoCr surfaces. CoCr was hydroxylated through alkalization in NaOH 5 M for 2 h. Subsequently, silanization was carried out using APTES. APTES was pre-mixed at 2% vol in isopropanol-water (200:1 v/v) and stirred for 1 h. After this, CoCr-OH samples were immersed in the APTES solution at room temperature for 1 min. The silane-coated samples were kept under curing conditions at 45 °C for 24 h. Then, silanized CoCr surfaces were immersed in 4 g/L GO aqueous suspension at 60 °C for 24 h. Carboxylic groups of graphene oxide in GO/CoCr samples were activated by immersing in distilled water (50 mL) with N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC, 250 mg) and N-hydroxysuccinimide (NHS, 750 mg) during 24 h under mild orbital saking. After that, the GO/CoCr surfaces with activated carboxyl groups were immersed in distilled water (100 mL) with dissolved adipic dihydrazide (ADH, 1.04 g) and let to react for another 24 h under mild orbital shaking. Pre-activated HA-(EDC/NHS) solution was obtained by dissolving HA (100 mg), EDC (253 mg) and NHS (754 mg) in 100 mL phosphate buffer saline (PBS) and stirred for 24h to activate the carboxylic acids of HA. After that, ADH/GO/CoCr surfaces were immersed in pre-activated HA-(EDC/NHS) solution and let to react for 24h under mild orbital motion (samples named HA/GO/CoCr). This data set includes the results of the final surface by Raman Spectroscopy and Electrochemical Impedance Spectroscopy. Raman was obtained from CoCr/GO/HA surfaces in comparison with GO/CoCr surfaces and HA powder. Electrochemical impedance data of CoCr/GO/HA were obtained under immersion in 3 g/L Hyaluronic acid solution up to 23 days., [ES] Este es el conjunto de datos experimentales utilizado en el artículo " Macrophage Proteomic Analysis of Covalent Immobilization of Hyaluronic Acid and Graphene Oxide on CoCr Alloy in a Tribocorrosive Environment". El artículo incluye el procedimiento experimental realizado para la deposición de óxido de grafeno y la posterior inmovilización de ácido hialurónico sobre superficies de CoCr. Las superficies de CoCr fueron hidroxiladas mediante alcalinización en NaOH 5 M durante 2 h. Posteriormente, se realizó la silanización utilizando APTES premezclado al 2% en volumen en isopropanol-agua (200:1 v/v) agitada durante 1 h. Las muestras de CoCr hidroxiladas se sumergieron en la solución de APTES a temperatura ambiente durante 1 minuto. Posteriormente, se mantuvieron en condiciones de curado a 45 °C durante 24 h. Transcurrido este tiempo, las superficies de CoCr silanizadas se sumergieron en una suspensión acuosa de 4g/L GO a 60 °C durante 24 h. Los grupos carboxílicos del óxido de grafeno en las muestras GO/CoCr se activaron mediante inmersión en 50 mL agua destilada que contenía hidrocloruro de N-(3-dimetilaminopropil)-N-etilcarbodiimida (EDC, 250 mg) y N-hidroxisuccinimida (NHS, 750 mg), durante 24 h en agitación orbital suave. Las superficies de GO/CoCr con grupos carboxílicos activados se sumergieron en 100 mL de agua destilada con dihidracida adipídica disuelta (ADH, 1.04 g) y se dejaron reaccionar durante otras 24 h en agitación orbital suave. La solución de HA preactivada (EDC/NHS) se obtuvo disolviendo HA (100 mg), EDC (253 mg) y NHS (754 mg) en 100 mL de solución salina de fosfato (PBS) y agitando durante 24 h para activar los ácidos carboxílicos de la HA. Después, las superficies de ADH/GO/CoCr se sumergieron en la solución de HA preactivada (EDC/NHS) y se dejaron reaccionar durante 24 h en movimiento orbital suave (muestras denominadas HA/GO/CoCr). Este conjunto de datos incluye los resultados de la caracterización superficial final mediante espectroscopía Raman y espectroscopia de impedancia electroquímica. Se obtuvieron espectros Raman de superficies de HA/GO/CoCr, GO/CoCr, y se compararon con GO y ácido hialurónico, utilizados como referencia. Los datos de impedancia electroquímica de CoCr/GO/HA se obtuvieron en inmersión en solución de 3 g/L ácido hialurónico hasta 23 días., We acknowledge financial support from the Ministerio de Ciencia, Innovación y Universidades (MICIN), "ERDF A way of making Europe” and “ESF Investing in your future" through projects RTI2018-101506-B-C31 and RTI2018-101506-B-C31 and the predoctoral grant PRE2019-090122., Peer reviewed